CACNA1E (NM_001205293) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BII; CACH6; CACNL1A6; Cav2.3; DEE69; EIEE69; gm139
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235385 representing NM_001205293 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGCTTCGGGGAGGCGGTGGTCGCCAGGCCAGGGTCCGGCGATGGAGACTCGGACCAGAGCAGGA ACCGGCAAGGAACCCCCGTGCCGGCCTCGGGGCAGGCGGCCGCCTACAAGCAGACGAAAGCACAGAGGGC GCGGACTATGGCTTTGTACAACCCCATTCCCGTCCGGCAGAACTGTTTCACCGTCAACAGATCCCTGTTC ATCTTCGGAGAAGATAACATTGTCAGGAAATATGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGTACA TGATCCTGGCCACCATCATTGCCAACTGCATCGTCCTGGCCCTGGAGCAGCATCTTCCTGAGGATGACAA GACCCCCATGTCCCGAAGACTGGAGAAGACAGAACCTTATTTCATTGGGATCTTTTGCTTTGAAGCTGGG ATCAAAATTGTGGCCCTGGGGTTCATCTTCCATAAGGGCTCTTACCTCCGCAATGGCTGGAATGTCATGG ACTTCATCGTGGTCCTCAGTGGCATCCTGGCCACTGCAGGAACCCACTTCAATACTCACGTGGACCTGAG GACCCTCCGGGCTGTGCGTGTCCTGCGGCCTTTGAAGCTCGTGTCAGGGATACCTAGCCTGCAGATTGTG TTGAAGTCCATCATGAAGGCCATGGTACCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCCTGA TGTTTGCTATCATTGGTTTGGAGTTCTACAGTGGCAAGTTACATCGAGCATGCTTCATGAACAATTCAGG TATTCTAGAAGGATTTGACCCCCCTCACCCATGTGGTGTGCAGGGCTGCCCAGCTGGTTATGAATGCAAG GACTGGATCGGCCCCAATGATGGGATCACCCAGTTTGATAACATCCTTTTTGCTGTGCTGACTGTCTTCC AGTGCATCACCATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTGGAA TTGGCTGTACTTCATCCCCCTCATCATCATTGGATCCTTCTTTGTTCTCAACCTAGTCCTGGGAGTGCTT TCCGGGGAATTTGCCAAAGAGAGAGAGAGAGTGGAGAACCGAAGGGCTTTCATGAAGCTGCGGCGCCAGC AGCAGATTGAGCGTGAGCTGAATGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTCGCTGA AGAAAATAAAAATGCTGGAACATCCGCCTTAGAAGTGCTTCGAAGGGCAACCATCAAGAGGAGCCGGACA GAGGCCATGACTCGAGACTCCAGTGATGAGCACTGTGTTGATATCTCCTCTGTGGGCACACCTCTGGCCC GAGCCAGTATCAAAAGTGCAAAGGTAGACGGGGTCTCTTATTTCCGGCACAAGGAAAGGCTTCTGCGCAT CTCCATTCGCCACATGGTTAAATCCCAGGTGTTTTACTGGATTGTGCTGAGCCTTGTGGCACTCAACACT GCCTGTGTGGCCATTGTCCATCACAACCAGCCCCAGTGGCTCACCCACCTCCTCTACTATGCAGAATTTC TGTTTCTGGGACTCTTCCTCTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCTCGCCTTTATTTTCA CTCTTCATTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGTATCTTTGAAGTGGTCTGGGCAATCTTC AGACCTGGTACGTCTTTTGGAATCAGTGTCTTGCGAGCCCTCCGGCTTCTAAGAATATTTAAAATAACCA AGTATTGGGCTTCCCTACGGAATTTGGTGGTCTCCTTGATGAGCTCAATGAAGTCTATCATCAGTTTGCT TTTCCTCCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTAGGAATGCAGTTATTTGGAGGCAGGTTTAAC TTTAATGATGGGACTCCTTCGGCAAATTTTGATACCTTCCCTGCAGCCATCATGACTGTGTTCCAGATCC TGACGGGTGAGGACTGGAATGAGGTGATGTACAATGGGATCCGCTCCCAGGGTGGGGTCAGCTCAGGCAT GTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGCAACTACACGCTACTGAATGTGTTCTTGGCT ATCGCTGTGGATAATCTCGCCAACGCCCAGGAACTGACCAAGGATGAACAGGAGGAAGAAGAGGCCTTCA ACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCACCCAACATGCCTTCGATCGA AAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGGAGG CGCCGGCACCACATGTCCGTGTGGGAGCAGCGTACCAGCCAGCTGAGGAAGCACATGCAGATGTCCAGCC AGGAGGCCCTCAACAGAGAGGAGGCGCCGACCATGAACCCGCTCAACCCCCTCAACCCGCTCAGCTCCCT CAACCCGCTCAATGCCCACCCCAGCCTTTATCGGCGACCCAGGGCCATTGAGGGCCTGGCCCTGGGCCTG GCCCTGGAGAAGTTCGAGGAGGAGCGCATCAGCCGTGGGGGGTCCCTCAAGGGGGATGGAGGGGACCGAT CCAGTGCCCTGGACAACCAGAGGACCCCTTTGTCCCTGGGCCAGCGGGAGCCACCATGGCTGGCCAGGCC CTGTCATGGAAACTGTGACCCGACTCAGCAGGAGGCAGGGGGAGGAGAGGCTGTGGTGACCTTTGAGGAC CGGGCCAGGCACAGGCAGAGCCAACGGCGCAGCCGGCATCGCCGCGTCAGGACAGAAGGCAAGGAGTCCT CTTCAGCCTCCCGGAGCAGGTCTGCCAGCCAGGAACGCAGTCTGGATGAAGCCATGCCCACTGAAGGGGA GAAGGACCATGAGCTCAGGGGCAACCATGGTGCCAAGGAGCCAACGATCCAAGAAGAGAGAGCCCAGGAT TTAAGGAGGACCAACAGTCTGATGGTGTCCAGAGGCTCCGGGCTGGCAGGAGGCCTTGATGAGGCTGACA CCCCCCTAGTCCTGCCCCATCCTGAGCTGGAAGTGGGGAAGCACGTGGTGCTGACGGAGCAGGAGCCAGA AGGCAGCAGTGAGCAGGCCCTGCTGGGGAATGTGCAGCTAGACATGGGCCGGGTCATCAGCCAGAGCGAG CCTGACCTCTCCTGCATCACGGCCAACACGGACAAGGCCACCACCGAGAGCACCAGCGTCACCGTCGCCA TCCCCGACGTGGACCCCTTGGTGGACTCAACCGTGGTGCACATTAGCAACAAGACGGATGGGGAAGCCAG TCCCTTGAAGGAGGCAGAGATCAGAGAGGATGAGGAGGAGGTGGAGAAGAAGAAGCAGAAGAAGGAGAAG CGTGAGACAGGCAAAGCCATGGTGCCCCACAGCTCAATGTTCATCTTCAGCACCACCAACCCGATCCGGA GGGCCTGCCACTACATCGTGAACCTGCGCTACTTTGAGATGTGCATCCTCCTGGTGATTGCAGCCAGCAG CATCGCCCTGGCGGCAGAGGACCCCGTCCTGACCAACTCGGAGCGCAACAAAGTCCTGAGGTATTTTGAC TATGTGTTCACGGGCGTGTTCACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGATCCTGCAGG ATGGGTCCTACTTCCGAGACTTGTGGAACATCCTGGACTTTGTGGTGGTCGTTGGCGCATTGGTGGCCTT TGCTCTGGCGAACGCTTTGGGAACCAACAAAGGACGGGACATCAAGACCATCAAGTCTCTGCGGGTGCTC CGAGTTCTAAGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTCAAGGCCGTCTTCGACTGCGTAGTGA CCTCCTTGAAGAATGTCTTCAACATACTCATTGTGTACAAGCTCTTCATGTTCATCTTTGCTGTCATCGC AGTTCAGCTCTTCAAGGGAAAGTTCTTTTATTGCACGGACAGTTCCAAGGACACAGAGAAGGAGTGCATA GGCAACTATGTAGATCATGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGCCATGAATTCC ACTACGACAACATTATCTGGGCCCTGCTGACCCTCTTCACCGTCTCCACAGGGGAAGGATGGCCTCAAGT TCTGCAGCACTCTGTAGATGTGACAGAGGAAGACCGAGGCCCAAGCCGCAGCAACCGCATGGAGATGTCT ATCTTTTATGTAGTCTACTTTGTGGTCTTCCCCTTCTTCTTTGTCAATATCTTTGTGGCTCTCATCATCA TCACCTTCCAGGAGCAAGGGGATAAGATGATGGAGGAGTGCAGCCTGGAGAAGAATGAGAGGGCGTGCAT CGACTTCGCCATCAGCGCCAAACCTCTCACCCGCTACATGCCGCAGAACAGACACACCTTCCAGTACCGC GTGTGGCACTTTGTGGTGTCTCCGTCCTTTGAGTACACCATTATGGCCATGATCGCCTTGAATACTGTTG TGCTGATGATGAAGTATTATTCTGCTCCCTGTACCTATGAGCTGGCCCTGAAGTACCTGAATATCGCCTT CACCATGGTGTTTTCCCTGGAATGTGTCCTGAAGGTCATCGCTTTTGGCTTTTTGAACTATTTCCGAGAC ACCTGGAATATCTTTGACTTCATCACCGTGATTGGCAGTATCACAGAAATTATCCTGACAGACAGCAAGC TGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCCCGCCTCATAAAGCTCCT GCGTCAGGGCTATACCATACGCATTTTGCTGTGGACCTTTGTGCAGTCCTTTAAGGCCCTCCCTTATGTC TGCCTTTTAATTGCCATGCTTTTCTTCATTTATGCCATCATTGGGATGCAGGTATTTGGAAACATAAAAT TAGACGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGGTCCCTAATGCTACTCTT CAGGAGTGCCACAGGTGAGGCCTGGCAGGAGATTATGCTGTCATGCCTTGGGGAGAAGGGCTGTGAGCCT GACACCACCGCACCATCAGGGCAGAACGAGAACGAACGCTGCGGCACCGATCTGGCCTACGTGTACTTTG TCTCCTTCATCTTCTTCTGCTCCTTCTTGATGCTCAACCTGTTTGTGGCCGTCATCATGGACAACTTTGA GTACCTGACTCGGGACTCCTCCATCCTGGGGCCTCACCACTTGGACGAGTTTGTCCGCGTCTGGGCAGAA TATGACCGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCATGTCACCTC CGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCATATAAGAGGTTGGTCCTGATGAACATGCCAGT AGCTGAGGACATGACGGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTGGACATTAAA ATTGCCAAAGGTGGTGCAGACAGGCAGCAGCTAGACTCAGAGCTACAAAAGGAGACCCTAGCCATCTGGC CTCACCTATCCCAGAAGATGCTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGACTGTGGGCAA AATCTATGCAGCAATGATGATCATGGACTACTATAAGCAGAGTAAGGTGAAGAAGCAGAGGCAGCAGCTG GAGGAACAGAAAAATGCCCCCATGTTCCAGCGCATGGAGCCTTCATCTCTGCCTCAGGAGATCATTGCTA ATGCCAAAGCCCTGCCTTACCTCCAGCAGGACCCCGTTTCAGGCCTGAGTGGCCGGAGTGGATACCCTTC GATGAGTCCACTCTCTCCCCAGGATATATTCCAGTTGGCTTGTATGGACCCCGCCGATGACGGACAGTTC CAAGAACGGCAGTCTCTGGAGCCAGAGGTTAGTGAATTAAAAAGCGTGCAGCCCTCTAACCATGGCATCT ACCTTCCTTCGGACACCCAGGAGCATGCGGGATCTGGGAGGGCATCTTCTATGCCACGTCTGACTGTGGA TCCCCAGGTGGTGACAGACCCTAGCTCCATGAGACGTTCATTTTCCACTATTCGGGATAAGCGTTCAAAT TCCTCGTGGTTGGAGGAATTCTCCATGGAGCGAAGCAGTGAAAATACCTACAAGTCCCGTCGCCGGAGTT ACCACTCCTCCTTGCGGCTGTCAGCCCACCGCCTGAACTCTGATTCAGGCCACAAGTCTGACACTCACCG CTCAGGGGGCAGGGAGCGGGGACGATCAAAAGAGCGAAAGCATCTTCTCTCTCCTGATGTCTCCCGCTGC AATTCAGAAGAGCGAGGGACCCAGGCTGACTGGGAGTCCCCAGAGCGCCGTCAATCCAGGTCACCCAGTG AGGGCAGGTCACAGACGCCCAACAGACAGGGCACAGGTTCCCTAAGTGAGAGCTCCATCCCCTCTGTCTC TGACACCAGCACCCCAAGAAGAAGTCGTCGGCAGCTCCCACCCGTCCCGCCAAAGCCCCGGCCCCTCCTT TCCTACAGCTCCCTGATTCGACACGCGGGCAGCATCTCTCCACCTGCTGATGGAAGCGAGGAGGGCTCCC CGCTGACCTCCCAAGCTCTGGAGAGCAACAATGCTTGCCTGACCGAGTCTTCCAACTCTCCGCACCCCCA GCAGAGCCAACATGCCTCCCCACAGCGCTACATCTCCGAGCCCTACTTGGCCCTGCACGAAGACTCCCAC GCCTCAGACTGTGGTGAGGAGGAGACGCTCACTTTCGAAGCAGCCGTGGCTACTAGCCTGGGCCGTTCCA ACACCATCGGCTCAGCCCCACCCCTGCGGCATAGCTGGCAGATGCCCAACGGGCACTATCGGCGGCGGAG GCGCGGGGGGCCTGGGCCAGGCATGATGTGTGGGGCTGTCAACAACCTGCTAAGTGACACGGAAGAAGAT GACAAATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235385 representing NM_001205293 Red=Cloning site Green=Tags(s) MARFGEAVVARPGSGDGDSDQSRNRQGTPVPASGQAAAYKQTKAQRARTMALYNPIPVRQNCFTVNRSLF IFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAG IKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIV LKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECK DWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVL SGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRT EAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGVSYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNT ACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIF RPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFN FNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLA IAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERR RRHHMSVWEQRTSQLRKHMQMSSQEALNREEAPTMNPLNPLNPLSSLNPLNAHPSLYRRPRAIEGLALGL ALEKFEEERISRGGSLKGDGGDRSSALDNQRTPLSLGQREPPWLARPCHGNCDPTQQEAGGGEAVVTFED RARHRQSQRRSRHRRVRTEGKESSSASRSRSASQERSLDEAMPTEGEKDHELRGNHGAKEPTIQEERAQD LRRTNSLMVSRGSGLAGGLDEADTPLVLPHPELEVGKHVVLTEQEPEGSSEQALLGNVQLDMGRVISQSE PDLSCITANTDKATTESTSVTVAIPDVDPLVDSTVVHISNKTDGEASPLKEAEIREDEEEVEKKKQKKEK RETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFD YVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVL RVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECI GNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMS IFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYR VWHFVVSPSFEYTIMAMIALNTVVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKVIAFGFLNYFRD TWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYV CLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEP DTTAPSGQNENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAE YDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIK IAKGGADRQQLDSELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQL EEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGRSGYPSMSPLSPQDIFQLACMDPADDGQF QERQSLEPEVSELKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSN SSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRC NSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLL SYSSLIRHAGSISPPADGSEEGSPLTSQALESNNACLTESSNSPHPQQSQHASPQRYISEPYLALHEDSH ASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGMMCGAVNNLLSDTEED DKC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205293
ORF Size	6939 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205293.3
RefSeq Size	15101 bp
RefSeq ORF	6942 bp
Locus ID	777
UniProt ID	Q15878
Protein Families	Druggable Genome, Ion Channels: Calcium, Transmembrane
Protein Pathways	Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus
MW	262.2 kDa
Gene Summary	Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]

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