NOLC1 (NM_001284388) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NOPP130; NOPP140; NS5ATP13; P130; Srp40
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239378 representing NM_001284388 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGTGCTCGGCTTCCTGCGCG ATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGACAGGAGCTACACAGCAGGATGCCAATGC CTCTTCCCTCTTAGACATCTATAGCTTCTGGCTCAAGTCTGCCAAGGTCCCAGAGCGAAAGTTACAGGCA AATGGACCAGTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAGGAGG AGGAGGAAGTTCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCTCCTGG GAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGATGAGGAGGAC CAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGCCAAAGCTCCTCCTAAGAAGG CCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGATGAGCCACCAAAGAACCAGAAGCCAAAGAT AACACCTGTGACAGTTAAAGCTCAGACTAAAGCCCCTCCCAAACCAGCTCGAGCAGCACCTAAAATAGCC AATGGTAAAGCAGCCAGTAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCAGAGGAGGAGA AGGCAGCAGCCACCCCCAAGAAGGTCTGGACCATAACTTCTGTCAGGGCAGAGACTGTACCTAAAAAGCA AGTTGTGGCCAAGGCCCCAGTGAAAGCAGCTACCACCCCTACCCGGAAGAGTTCTAGCAGTGAGGATTCC TCCAGTGACGAGGAAGAGGAGCAAAAAAAACCCATGAAAAATAAACCAGGTCCCTACAGTTCAGTCCCCC CGCCTTCTGCTCCCCCACCAAAGAAGTCTCTGGGAACCCAGCCTCCCAAGAAGGCTGTGGAGAAGCAGCA GCCTGTGGAAAGCAGTGAAGACAGCAGTGATGAGTCTGATTCAAGTTCTGAAGAAGAGAAGAAACCCCCA ACTAAGGCAGTAGTCTCTAAAGCAACCACTAAACCACCTCCAGCAAAGAAAGCAGCAGAGAGCTCTTCAG ACAGCTCAGACTCTGACAGCTCTGAGGATGATGAAGCTCCTTCTAAGCCAGCTGGTACCACCAAGAATTC TTCAAATAAGCCAGCTGTCACCACCAAGTCACCTGCAGTGAAGCCAGCTGCAGCCCCCAAGCAACCTGTG GGCGGTGGCCAGAAGCTTCTGACGAGAAAGGCTGACAGCAGCTCCAGTGAGGAAGAGAGCAGCTCCAGTG AGGAGGAGAAGACAAAGAAGATGGTGGCCACCACTAAGCCCAAGGCGACTGCCAAAGCAGCTCTATCTCT GCCTGCCAAGCAGGCTCCTCAGGGTAGTAGGGACAGCAGCTCTGATTCAGACAGCTCCAGCAGTGAGGAG GAGGAAGAGAAGACATCTAAGTCTGCAGTTAAGAAGAAGCCACAGAAGGTAGCAGGAGGTGCAGCCCCTT CCAAGCCAGCCTCTGCAAAGAAAGGAAAGGCTGAGAGCAGCAACAGTTCTTCTTCTGATGACTCCAGTGA GGAAGAGGAAGAGAAGCTCAAGGGCAAGGGCTCTCCAAGACCACAAGCCCCCAAGGCCAATGGCACCTCT GCACTGACTGCCCAGAATGGAAAAGCAGCTAAGAACAGTGAGGAGGAGGAAGAAGAAAAGAAAAAGGCGG CAGTGGTAGTTTCCAAATCAGGTTCATTAAAGAAGCGGAAGCAGAATGAGGCTGCCAAGGAGGCAGAGAC TCCTCAGGCCAAGAAGATAAAGCTTCAGACCCCTAACACATTTCCAAAAAGGAAGAAAGGAGAAAAAAGG GCATCATCCCCATTCCGAAGGGTCAGGGAGGAGGAAATTGAGGTGGATTCACGAGTTGCGGACAACTCCT TTGATGCCAAGCGAGGTGCAGCCGGAGACTGGGGAGAGCGAGCCAATCAGGTTTTGAAGTTCACCAAAGG CAAGTCCTTTCGGCATGAGAAAACCAAGAAGAAGCGGGGCAGCTACCGGGGAGGCTCAATCTCTGTCCAG GTCAATTCTATTAAGTTTGACAGCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239378 representing NM_001284388 Red=Cloning site Green=Tags(s) MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKSAKVPERKLQA NGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEED QKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKIA NGKAASSSSSSSSSSSSDDSEEEKAAATPKKVWTITSVRAETVPKKQVVAKAPVKAATTPTRKSSSSEDS SSDEEEEQKKPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPP TKAVVSKATTKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPV GGGQKLLTRKADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEE EEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTS ALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKR ASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQ VNSIKFDSE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NM_001284388
ORF Size	2127 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284388.1, NP_001271317.1
RefSeq Size	3977 bp
RefSeq ORF	2130 bp
Locus ID	9221
UniProt ID	Q14978
Protein Families	Stem cell - Pluripotency
MW	75.2 kDa
Gene Summary	Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function]

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