SUV420h1 (KMT5B) (NM_001300907) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CGI-85; CGI85; MRD51; SUV420H1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239387 representing NM_001300907 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAGAAATTATTCAAAGAACATGTATTTATTTATTTGCGAATGTTTGCAACTGACAGTGGATTTG AAATATTGCCATGTAATAGATACTCATCAGAACAAAATGGAGCCAAAATAGTTGCAACAAAAGAGTGGAA ACGAAATGACAAAATAGAATTACTGGTGGGTTGTATTGCCGAACTTTCAGAAATTGAGGAGAACATGCTA CTTAGACATGGAGAAAACGACTTCAGTGTCATGTACTCCACAAGGAAAAACTGTGCTCAACTCTGGCTGG GTCCTGCTGCGTTTATAAACCATGATTGCAGACCTAATTGTAAGTTTGTGTCAACTGGTCGAGATACAGC ATGTGTGAAGGCTCTAAGAGACATTGAACCTGGAGAAGAAATTTCTTGTTATTATGGAGATGGGTTCTTT GGAGAAAATAATGAGTTCTGCGAGTGTTACACTTGCGAAAGACGGGGCACTGGTGCTTTTAAATCCAGAG TGGGACTGCCTGCGCCTGCTCCTGTTATCAATAGCAAATATGGACTCAGAGAAACAGATAAACGTTTAAA TAGGCTTAAAAAGTTAGGTGACAGCAGCAAAAATTCAGACAGTCAATCTGTCAGCTCTAACACTGATGCA GATACCACTCAGGAAAAAAACAATGCAACTTCTAACCGAAAATCTTCAGTTGGCGTAAAAAAGAATAGCA AGAGCAGAACGTTAACGAGGCAATCTATGTCAAGAATTCCAGCTTCTTCCAACTCTACCTCATCTAAGCT AACTCATATAAATAATTCCAGGGTACCAAAGAAACTGAAGAAGCCTGCAAAGCCTTTACTTTCAAAGATA AAATTGAGAAATCATTGCAAGCGGCTGGAGCAAAAGAATGCTTCAAGAAAACTCGAAATGGGAAACTTAG TACTGAAAGAGCCTAAAGTAGTTCTGTATAAAAATTTGCCCATTAAAAAAGATAAGGAGCCAGAGGGACC AGCCCAAGCCGCAGTTGCCAGCGGGTGCTTGACTAGACACGCGGCGAGAGAACACAGACAGAATCCTGTG AGAGGTGCTCATTCGCAGGGGGAGAGCTCGCCCTGCACCTACATAACTCGGCGGTCAGTGAGGACAAGAA CAAATCTGAAGGAGGCCTCTGACATCAAGCTTGAACCAAATACGTTGAATGGCTATAAAAGCAGTGTGAC GGAACCTTGCCCCGACAGTGGTGAACAGCTGCAGCCAGCTCCTGTGCTGCAGGAGGAAGAACTGGCTCAT GAGACTGCACAAAAAGGGGAGGCAAAGTGTCATAAGAGTGACACAGGCATGTCCAAAAAGAAGTCACGAC AAGGAAAACTTGTGAAACAGTTTGCAAAAATAGAGGAATCTACTCCAGTGCACGATTCTCCTGGAAAAGA CGACGCGGTACCAGATTTGATGGGTCCCCATTCTGACCAGGGTGAGCACAGTGGCACTGTGGGCGTGCCT GTGAGCTACACAGACTGTGCTCCTTCACCCGTCGGTTGTTCAGTTGTGACATCAGATAGCTTCAAAACAA AAGACAGCTTTAGAACTGCAAAAAGTAAAAAGAAGAGGCGAATCACAAGGTATGATGCACAGTTAATCCT AGAAAATAACTCTGGGATTCCCAAATTGACTCTTCGTAGGCGTCATGATAGCAGCAGCAAAACAAATGAC CAAGAGAATGATGGAATGAACTCTTCCAAAATAAGCATCAAGTTAAGCAAAGACCATGACAACGATAACA ATCTCTATGTAGCAAAGCTTAATAATGGATTTAACTCAGGATCAGGCAGTAGTTCTACAAAATTAAAAAT CCAGCTAAAACGAGATGAGGAAAATAGGGGGTCTTATACAGAGGGGCTTCATGAAAATGGGGTGTGCTGC AGTGATCCTCTTTCTCTCTTGGAGTCTCGAATGGAGGTGGATGACTATAGTCAGTATGAGGAAGAAAGTA CAGATGATTCCTCCTCTTCTGAGGGCGATGAAGAGGAGGATGACTATGATGATGACTTTGAAGACGATTT TATTCCTCTTCCTCCAGCTAAGCGCTTGAGGTTAATAGTTGGAAAAGACTCTATAGATATTGACATTTCT TCAAGGAGAAGAGAAGATCAGTCTTTAAGGCTTAATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239387 representing NM_001300907 Red=Cloning site Green=Tags(s) MQEKLFKEHVFIYLRMFATDSGFEILPCNRYSSEQNGAKIVATKEWKRNDKIELLVGCIAELSEIEENML LRHGENDFSVMYSTRKNCAQLWLGPAAFINHDCRPNCKFVSTGRDTACVKALRDIEPGEEISCYYGDGFF GENNEFCECYTCERRGTGAFKSRVGLPAPAPVINSKYGLRETDKRLNRLKKLGDSSKNSDSQSVSSNTDA DTTQEKNNATSNRKSSVGVKKNSKSRTLTRQSMSRIPASSNSTSSKLTHINNSRVPKKLKKPAKPLLSKI KLRNHCKRLEQKNASRKLEMGNLVLKEPKVVLYKNLPIKKDKEPEGPAQAAVASGCLTRHAAREHRQNPV RGAHSQGESSPCTYITRRSVRTRTNLKEASDIKLEPNTLNGYKSSVTEPCPDSGEQLQPAPVLQEEELAH ETAQKGEAKCHKSDTGMSKKKSRQGKLVKQFAKIEESTPVHDSPGKDDAVPDLMGPHSDQGEHSGTVGVP VSYTDCAPSPVGCSVVTSDSFKTKDSFRTAKSKKKRRITRYDAQLILENNSGIPKLTLRRRHDSSSKTND QENDGMNSSKISIKLSKDHDNDNNLYVAKLNNGFNSGSGSSSTKLKIQLKRDEENRGSYTEGLHENGVCC SDPLSLLESRMEVDDYSQYEEESTDDSSSSEGDEEEDDYDDDFEDDFIPLPPAKRLRLIVGKDSIDIDIS SRRREDQSLRLNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001300907
ORF Size	2139 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300907.1, NP_001287836.1
RefSeq Size	5835 bp
RefSeq ORF	2142 bp
Locus ID	51111
Protein Families	Druggable Genome
Protein Pathways	Lysine degradation
MW	80 kDa
Gene Summary	This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

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