INPP5B (NM_001297434) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5PTase
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239478 representing NM_001297434 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGCCGCCGCCGGCTCTCGGGAGCGCAACACTGCAGGTGGTTCTAACTTTGATGGTTTGAGACCAA ATGGGAAGGGAGTGCCTATGGACCAAAGCTCCAGGGGTCAAGATAAACCAGAAAGCTTGCAACCAAGACA GAATAAATCCAAGTCCGAAATTACTGACATGGTTCGCTCCTCCACTATCACAGTGTCGGACAAGGCTCAT ATTTTATCCATGCAGAAGTTTGGACTGCGAGATACAATTGTGAAATCACATCTACTACAGAAAGAAGAGG ATTACACCTATATCCAGAACTTCAGGTTTTTTGCGGGAACATACAATGTAAATGGGCAGTCCCCCAAAGA ATGCCTCCGGCTGTGGCTGAGCAATGGTATCCAGGCCCCAGATGTCTATTGTGTAGGGTTCCAGGAGCTT GATCTGAGTAAGGAAGCTTTTTTCTTTCACGATACCCCAAAGGAGGAAGAGTGGTTCAAAGCTGTGTCAG AGGGTCTTCATCCAGATGCCAAATATGCAAAGGTGAAGCTTATCCGACTGGTTGGGATTATGCTGCTGTT ATATGTCAAACAGGAGCATGCAGCTTATATCTCAGAAGTGGAAGCCGAGACTGTGGGGACAGGAATCATG GGGAGGATGGGCAACAAGGGAGGCGTGGCGATCAGGTTCCAGTTCCACAACACCAGCATCTGCGTTGTGA ATTCTCACTTGGCAGCCCACATTGAAGAGTATGAGAGGAGGAACCAGGACTATAAGGACATTTGTTCTCG AATGCAGTTTTGTCAGCCTGACCCAAGCCTTCCCCCTCTCACCATCAGCAACCATGATGTGATCTTGTGG CTGGGGGACCTCAACTACAGGATAGAAGAGCTGGATGTGGAAAAAGTGAAAAAGCTCATCGAAGAGAAGG ACTTTCAAATGCTGTATGCATATGATCAGCTGAAAATTCAGGTGGCCGCAAAGACTGTCTTTGAAGGCTT CACAGAGGGTGAGCTCACATTCCAGCCTACTTACAAGTATGATACGGGCTCTGACGACTGGGATACCAGT GAGAAGTGCCGTGCTCCTGCCTGGTGTGATCGGATTCTCTGGAAAGGGAAGAACATCACTCAGCTGAGTT ACCAGAGCCACATGGCCCTGAAGACCAGTGACCACAAGCCTGTCAGCTCAGTGTTTGACATCGGGGTGAG GGTCGTAAATGACGAGCTTTACCGGAAGACACTGGAGGAAATTGTTCGCTCCCTGGATAAGATGGAAAAT GCCAACATTCCTTCTGTGTCCCTGTCCAAGCGAGAGTTCTGTTTTCAGAATGTGAAGTACATGCAATTGA AAGTAGAATCCTTTACAATTCATAATGGACAAGTACCCTGTCATTTTGAATTCATCAACAAGCCTGATGA AGAGTCTTACTGTAAGCAGTGGCTGAATGCCAACCCCAGCAGAGGCTTCCTCCTGCCAGATTCTGATGTT GAGATTGACTTGGAGCTCTTCGTAAATAAGATGACAGCTACAAAGCTCAACTCGGGTGAAGACAAAATTG AGGACATTCTGGTTCTGCACTTGGACAGGGGAAAGGATTACTTTTTGTCTGTGTCTGGGAACTACCTGCC CAGCTGTTTTGGGTCTCCCATTCATACACTGTGTTACATGAGAGAGCCAATCTTGGACCTACCACTTGAA ACCATTAGTGAGCTGACTCTGATGCCAGTATGGACTGGAGATGATGGGAGCCAGTTGGATAGCCCCATGG AAATCCCCAAAGAGCTCTGGATGATGGTTGATTACCTGTACCGAAATGCTGTCCAGCAGGAAGATCTGTT TCAGCAACCAGGCCTGAGGTCAGAATTTGAACATATCAGGGACTGCTTGGATACTGGAATGATTGATAAC CTCTCTGCCAGCAATCATTCTGTAGCCGAAGCCCTGCTGCTTTTCCTGGAGAGCCTTCCAGAGCCTGTCA TCTGTTACAGCACCTACCATAACTGCTTGGAGTGTTCTGGCAACTACACAGCAAGCAAACAGGTCATTTC TACTCTCCCCATATTCCACAAAAATGTCTTCCACTACTTGATGGCGTTTTTGCGAGAACTGCTGAAAAAT TCAGCAAAAAATCATTTGGATGAGAATATTCTAGCTAGCATATTTGGCAGCTTATTGCTTCGAAACCCAG CTGGTCACCAAAAGCTTGATATGACAGAGAAGAAGAAGGCTCAAGAATTTATTCACCAGTTCCTCTGCAA CCCACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239478 representing NM_001297434 Red=Cloning site Green=Tags(s) MSAAAGSRERNTAGGSNFDGLRPNGKGVPMDQSSRGQDKPESLQPRQNKSKSEITDMVRSSTITVSDKAH ILSMQKFGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQEL DLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIM GRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILW LGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTS EKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMEN ANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDV EIDLELFVNKMTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLE TISELTLMPVWTGDDGSQLDSPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRSEFEHIRDCLDTGMIDN LSASNHSVAEALLLFLESLPEPVICYSTYHNCLECSGNYTASKQVISTLPIFHKNVFHYLMAFLRELLKN SAKNHLDENILASIFGSLLLRNPAGHQKLDMTEKKKAQEFIHQFLCNPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297434
ORF Size	2247 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297434.2
RefSeq Size	3962 bp
RefSeq ORF	2250 bp
Locus ID	3633
UniProt ID	P32019
Protein Families	Druggable Genome
Protein Pathways	Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
MW	86.1 kDa
Gene Summary	This gene encodes a member of a family of inositol polyphosphate-5-phosphatases. These enzymes function in the regulation of calcium signaling by inactivating inositol phosphates. The encoded protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jul 2014]

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