Angiomotin like 1 (AMOTL1) (NM_001301007) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	JEAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239759 representing NM_001301007 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGTTGAAGATCCTCTTTGTAACT TCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAAC AGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCC ATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACC TCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACACCAGGT GGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGAACTGCCCACT TACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAGGGGGCGG TGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCCACTGTGAA CCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCG CTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCCGGCTCGGGGA ATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGCTGGACCCTCG GGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAGACCCAGGAGCAC GGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTACCCTGCTCCTCAGC CTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGAGCCGCCCATG CCAACTTCCGTTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACCTCTTCCGCCAGCGGG CCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCCCCGCCTGCCG CCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAATGGTGGAGAT ATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGACAATGCCGACAAGCTCCAC AAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTGGTCAAGTCTACCACCAAGCGAG AATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGCGAGATTAGAAGACTTCATGATTTCAACAGAGA CCTCCGAGATCGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAATACGAAGGGCATGAAGACAAA GCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTAGAAATGGAGT TAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATCCTGGACCAGGCTTTGAGCAA CGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAGCAAGCATATGTTGAGAAAGTTGAG AAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAGCGAGAACAGATGGAGCGGAGACTGC GGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACCCAGCAGAAACATGGAAATGGCCAGCCAGCCAA CATGCCGGAATACAATGCCCCAGCCCTCCTGGAACTTGTGCGGGAGAAGGAGGAGCGGATCCTGGCCCTG GAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTTGCCATGAATG CCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCACGGAATGGCAGCTACGGAGA GAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAGGTGGTGCAGGCCAACAGAAGGTGTCAGGAC ATGGAATACACTATTAAAAATCTCCATGCCAAAATCATAGAGAAAGATGCTATGATTAAGGTCCTGCAGC AGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCCAGCCTACGTCCTGCCCGCTCCGTTCCATCCAT AGCAGCAGCTACTGGGACACACTCTCGCCAGACCTCTCTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAG GAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCATGCCTCTGCCC CACTGCTGCCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACTACGGCAGCCAGCAGTGCCCA CGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGACAAGAGTGCCGAGCTCTTCTGGCCCAGCATG GCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACGACCCCTGCTCACAGCCCCGTCCTGAAACACCCAGCGG CCAAAGGGACCGCAGAGAAACTGGAGAACTCTCCTGGCCATGGGAAGTCGCCTGACCACAGAGGCCGGGT CAGCAGCTTGCTGCACAAGCCCGAGTTCCCTGATGGAGAGATGATGGAAGTCCTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239759 representing NM_001301007 Red=Cloning site Green=Tags(s) MWRAKLRRGTCEPAVKVEDPLCNFHSPNFLRISEVEMRGSEDAAAGTVLQRLIQEQLRYGTPTENMNLLA IQHQATGSAGPAHPTNNFSSTENLTQEDPQMVYQSARQEPQGQEHQVDNTVMEKQVRSTQPQQNNEELPT YEEAKAQSQFFRGQQQQQQQQGAVGHGYYMAGGTSQKSRTEGRPTVNRANSGQAHKDEALKELKQGHVRS LSERIMQLSLERNGAKQHLPGSGNGKGFKVGGGPSPAQPAGKVLDPRGPPPEYPFKTKQMMSPVSKTQEH GLFYGDQHPGMLHEMVKPYPAPQPVRTDVAVLRYQPPPEYGVTSRPCQLPFPSTMQQHSPMSSQTSSASG PLHSVSLPLPLPMALGAPQPPPAASPSQQLGPDAFAIVERAQQMVEILTEENRVLHQELQGYYDNADKLH KFEKELQRISEAYESLVKSTTKRESLDKAMRNKLEGEIRRLHDFNRDLRDRLETANRQLSSREYEGHEDK AAEGHYASQNKEFLKEKEKLEMELAAVRTASEDHRRHIEILDQALSNAQARVIKLEEELREKQAYVEKVE KLQQALTQLQSACEKREQMERRLRTWLERELDALRTQQKHGNGQPANMPEYNAPALLELVREKEERILAL EADMTKWEQKYLEESTIRHFAMNAAATAAAERDTTIINHSRNGSYGESSLEAHIWQEEEEVVQANRRCQD MEYTIKNLHAKIIEKDAMIKVLQQRSRKDAGKTDSSSLRPARSVPSIAAATGTHSRQTSLTSSQLAEEKK EEKTWKGSIGLLLGKEHHEHASAPLLPPPPTSALSSIASTTAASSAHAKTGSKDSSTQTDKSAELFWPSM ASLPSRGRLSTTPAHSPVLKHPAAKGTAEKLENSPGHGKSPDHRGRVSSLLHKPEFPDGEMMEVLI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301007
ORF Size	2718 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301007.2
RefSeq Size	8849 bp
RefSeq ORF	2721 bp
Locus ID	154810
UniProt ID	Q8IY63
Protein Pathways	Tight junction
MW	101.6 kDa
Gene Summary	The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

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