NLRX1 (NM_001282144) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CLR11.3; DLNB26; NOD5; NOD9; NOD26
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239839 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTGGGGCCACCATTTGCCCAGGGCCTCTTGGGGCTCTGGTTTTAGAAGAGCACTCCAGCGACCAG ATGATCGTATCCCCTTCCTGATCCACTGGAGTTGGCCCCTTCAAGGGGAGCGTCCCTTTGGGCCCCCTAG GGCCTTTATACGCCACCACGGAAGCTCGGTAGATAGCGCTCCCCCACCCGGGAGGCATGGACGGCTGTTC CCCAGCGCCTCTGCAACTGAAGCTATACAGCGGCACCGCCGGAACCTGGCTGAGTGGTTCAGCCGGCTGC CCAGGGAGGAGCGCCAGTTTGGCCCAACCTTTGCCCTAGACACGGTCCACGTTGACCCTGTGATCCGCGA GAGTACCCCTGATGAGCTACTTCGCCCACCCGCGGAGCTGGCCCTGGAGCATCAGCCACCCCAGGCCGGG CTCCCCCCACTGGCCTTGTCTCAGCTCTTTAACCCGGATGCCTGTGGGCGCCGGGTGCAGACAGTGGTGC TGTATGGGACAGTGGGCACAGGCAAGAGCACGCTGGTGCGCAAGATGGTTCTGGACTGGTGTTATGGGCG GCTGCCGGCCTTCGAGCTGCTCATCCCCTTCTCCTGTGAGGACCTGTCATCCCTGGGCCCTGCCCCAGCC TCCCTGTGCCAACTTGTGGCCCAGCGCTACACGCCCCTGAAGGAGGTTCTGCCCCTGATGGCTGCTGCTG GGTCCCACCTCCTCTTTGTGCTCCATGGCTTAGAGCATCTCAACCTCGACTTCCGGCTGGCAGGCACGGG ACTTTGTAGTGACCCGGAGGAACCGCAGGAACCAGCTGCTATCATCGTCAACCTGCTGCGCAAATACATG CTGCCTCAGGCCAGCATTCTGGTGACCACTCGGCCCTCTGCCATTGGCCGTATCCCCAGCAAGTACGTGG GCCGCTATGGTGAGATCTGCGGTTTCTCTGATACCAACCTGCAGAAGCTCTACTTCCAGCTCCGCCTCAA CCAGCCGTACTGCGGGTATGCCGTTGGCGGTTCAGGTGTCTCTGCCACACCAGCTCAGCGTGACCACCTG GTGCAGATGCTCTCCCGGAACCTGGAGGGGCACCACCAGATAGCCGCTGCCTGCTTCCTGCCGTCCTATT GCTGGCTCGTTTGTGCCACCTTGCACTTCCTGCATGCCCCCACGCCTGCTGGGCAGACCCTTACAAGCAT CTATACCAGCTTCCTGCGCCTCAACTTCAGCGGGGAAACCCTGGACAGCACTGACCCCTCCAATTTGTCC CTGATGGCCTATGCAGCCCGAACCATGGGCAAGTTGGCCTATGAGGGGGTGTCCTCCCGCAAGACCTACT TCTCTGAAGAGGATGTCTGTGGCTGCCTGGAGGCTGGCATCAGGACGGAGGAGGAGTTTCAGCTGCTGCA CATCTTCCGTCGGGATGCCCTGAGGTTTTTCCTGGCCCCATGTGTGGAGCCAGGGCGTGCAGGCACCTTC GTGTTCACCGTGCCCGCCATGCAGGAATACCTGGCTGCCCTCTACATTGTGCTGGGTTTGCGCAAGACGA CCCTGCAAAAGGTGGGCAAGGAAGTGGCTGAGCTCGTGGGCCGTGTTGGGGAGGACGTCAGCCTGGTACT GGGCATCATGGCCAAGCTGCTGCCTCTGCGGGCTCTGCCTCTGCTCTTCAACCTGATCAAGGTGGTTCCA CGAGTGTTTGGGCGCATGGTGGGTAAAAGCCGGGAGGCGGTGGCTCAGGCCATGGTGCTGGAGATGTTTC GAGAGGAGGACTACTACAACGATGATGTTCTGGACCAGATGGGCGCCAGTATCCTGGGCGTGGAGGGCCC CCGGCGCCACCCAGATGAGCCCCCTGAGGATGAAGTCTTCGAGCTCTTCCCCATGTTCATGGGGGGGCTT CTCTCTGCCCACAACCGAGCTGTGCTAGCTCAGCTTGGCTGCCCCATCAAGAACCTGGATGCCCTGGAGA ATGCCCAGGCCATCAAGAAGAAGCTGGGCAAGCTGGGCCGGCAGGTGCTGCCCCCATCAGAGCTCCTTGA CCACCTCTTCTTCCACTATGAGTTCCAGAACCAGCGCTTCTCCGCTGAGGTGCTCAGCTCCCTGCGTCAG CTCAACCTGGCAGGTGTGCGCATGACACCAGTCAAGTGCACAGTGGTGGCAGCTGTGCTGGGCAGCGGAA GGCATGCCCTGGATGAGGTGAACTTGGCCTCCTGCCAGCTAGATCCTGCTGGGCTGCGCACACTCCTGCC TGTCTTCCTGCGTGCCCGGAAGCTGGGCTTGCAACTCAACAGCCTGGGCCCTGAGGCCTGCAAGGACCTC CGAGACCTGTTGCTGCATGACCAGTGCCAAATTACCACACTGCGGCTGTCCAACAACCCGCTGACGGCGG CAGGTGTTGCCGTGCTAATGGAGGGGCTGGCAGGAAACACCTCAGTGACGCACCTGTCCCTGCTGCACAC GGGCCTTGGGGACGAAGGCCTGGAGCTGCTGGCTGCCCAGCTGGACCGCAACCGGCAGCTGCAGGAGCTG AACGTGGCGTACAACGGTGCTGGTGACACAGCGGCCCTGGCCCTGGCCAGAGCTGCCCGGGAGCACCCTT CCCTGGAACTGCTACACCTCTACTTCAATGAGCTGAGCTCAGAGGGCCGCCAGGTCTTGCGAGACTTGGG GGGTGCTGCTGAAGGTGGTGCCCGGGTGGTGGTGTCACTGACAGAGGGGACGGCGGTGTCAGAATACTGG TCAGTGATCCTCAGTGAAGTCCAGCGGAACCTCAATAGCTGGGATCGGGCCCGGGTTCAGCGACACCTTG AGCTCCTACTGCGGGATCTGGAAGATAGCCGGGGTGCCACCCTTAATCCTTGGCGCAAGGCCCAGCTGCT GCGAGTGGAGGGCGAGGTCAGGGCCCTCCTGGAGCAGCTGGGAAGCTCTGGAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239839 protein sequence Red=Cloning site Green=Tags(s) MRWGHHLPRASWGSGFRRALQRPDDRIPFLIHWSWPLQGERPFGPPRAFIRHHGSSVDSAPPPGRHGRLF PSASATEAIQRHRRNLAEWFSRLPREERQFGPTFALDTVHVDPVIRESTPDELLRPPAELALEHQPPQAG LPPLALSQLFNPDACGRRVQTVVLYGTVGTGKSTLVRKMVLDWCYGRLPAFELLIPFSCEDLSSLGPAPA SLCQLVAQRYTPLKEVLPLMAAAGSHLLFVLHGLEHLNLDFRLAGTGLCSDPEEPQEPAAIIVNLLRKYM LPQASILVTTRPSAIGRIPSKYVGRYGEICGFSDTNLQKLYFQLRLNQPYCGYAVGGSGVSATPAQRDHL VQMLSRNLEGHHQIAAACFLPSYCWLVCATLHFLHAPTPAGQTLTSIYTSFLRLNFSGETLDSTDPSNLS LMAYAARTMGKLAYEGVSSRKTYFSEEDVCGCLEAGIRTEEEFQLLHIFRRDALRFFLAPCVEPGRAGTF VFTVPAMQEYLAALYIVLGLRKTTLQKVGKEVAELVGRVGEDVSLVLGIMAKLLPLRALPLLFNLIKVVP RVFGRMVGKSREAVAQAMVLEMFREEDYYNDDVLDQMGASILGVEGPRRHPDEPPEDEVFELFPMFMGGL LSAHNRAVLAQLGCPIKNLDALENAQAIKKKLGKLGRQVLPPSELLDHLFFHYEFQNQRFSAEVLSSLRQ LNLAGVRMTPVKCTVVAAVLGSGRHALDEVNLASCQLDPAGLRTLLPVFLRARKLGLQLNSLGPEACKDL RDLLLHDQCQITTLRLSNNPLTAAGVAVLMEGLAGNTSVTHLSLLHTGLGDEGLELLAAQLDRNRQLQEL NVAYNGAGDTAALALARAAREHPSLELLHLYFNELSSEGRQVLRDLGGAAEGGARVVVSLTEGTAVSEYW SVILSEVQRNLNSWDRARVQRHLELLLRDLEDSRGATLNPWRKAQLLRVEGEVRALLEQLGSSGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282144
ORF Size	2925 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282144.2
RefSeq Size	4158 bp
RefSeq ORF	2928 bp
Locus ID	79671
UniProt ID	Q86UT6
Protein Pathways	RIG-I-like receptor signaling pathway
MW	107.6 kDa
Gene Summary	The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]

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