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MRI1 (NM_001031727) Human Tagged ORF Clone
CAT＃: RG200993
- TrueORF®
MRI1 (tGFP-tagged) - Human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1
ORF Plasmid: DDK
"NM_001031727" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | M1Pi; MRDI; MTNA; Ypr118w |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG200993 representing NM_001031727
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCTGGAGGCGATCCGCTACTCGCGGGGCTCCCTGCAGATCCTAGACCAGCTGCTGCTGCCCAAGC AGAGCCGCTACGAGGCGGTGGGCTCGGTGCACCAGGCCTGGGAGGCCATCCGCGCCATGAAGGTGCGGGG CGCCCCGGCCATAGCCCTGGTGGGCTGTCTCAGCCTCGCCGTGGAGCTGCAGGCGGGCGCCGGGGGACCG GGACTCGCCGCGCTCGTGGCCTTCGTGCGCGACAAGCTGAGCTTCCTCGTCACCGCCCGGCCCACCGCTG TCAACATGGCCCGCGCCGCCCGCGACCTGGCTGATGTTGCAGCCCGGGAGGCCGAACGGGAGGGCGCTAC GGAAGAGGCGGTCCGGGAGAGAGTGATCTGCTGCACCGAGGACATGCTGGAGAAAGACCTCAGAGACAAC CGAAGCATTGGGGACCTAGGAGCCCGCCACCTCCTGGAGCGGGTGGCCCCCAGCGGTGGCAAGGTGACTG TGCTGACCCACTGTAACACTGGTGCTCTGGCCACCGCTGGCTATGGTACAGCCCTAGGTGTGATTCGCTC ACTGCACAGCCTGGGCCGCCTGGAGCATGCCTTCTGCACAGAGACCCGGCCCTACAACCAGGGAGCCCGG CTGACGGCCTTTGAGCTGGTCTATGAGCAGATCCCCGCCACCCTTATCACCGACAGCATGGTGGCTGCTG CCATGGCCCATAGGGGCGTGTCAGCTGTGGTCGTGGGAGCTGACCGCGTGGTTGCCAACGGCGACACAGC CAACAAGGTGGGCACCTACCAGCTGGCCATTGTCGCCAAGCACCATGGCATTCCCTTCTACGTGGCTGCC CCCAGCTCTTCATGTGACCTCCGTCTGGAGACCGGCAAGGAGATCATTATTGAAGAGCGACCGGGCCAGG AGCTGACCGATGTTAATGGGGTCCGGATTGCAGCACCTGGGATTGGAGTTTGGAATCCTGCCTTCGATGT CACCCCCCACGACCTCATCACTGGTGGCATCATCACAGAACTGGGGGTCTTTGCCCCTGAGGAGCTCCGG ACAGCCCTAACCACCACCATCTCTTCCAGGGATGGAACCCTAGATGGACCCCAGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200993 representing NM_001031727
Red=Cloning site Green=Tags(s) MTLEAIRYSRGSLQILDQLLLPKQSRYEAVGSVHQAWEAIRAMKVRGAPAIALVGCLSLAVELQAGAGGP GLAALVAFVRDKLSFLVTARPTAVNMARAARDLADVAAREAEREGATEEAVRERVICCTEDMLEKDLRDN RSIGDLGARHLLERVAPSGGKVTVLTHCNTGALATAGYGTALGVIRSLHSLGRLEHAFCTETRPYNQGAR LTAFELVYEQIPATLITDSMVAAAMAHRGVSAVVVGADRVVANGDTANKVGTYQLAIVAKHHGIPFYVAA PSSSCDLRLETGKEIIIEERPGQELTDVNGVRIAAPGIGVWNPAFDVTPHDLITGGIITELGVFAPEELR TALTTTISSRDGTLDGPQM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001031727 |
| ORF Size | 1107 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001031727.4 |
| RefSeq Size | 3177 bp |
| RefSeq ORF | 1110 bp |
| Locus ID | 84245 |
| UniProt ID | Q9BV20 |
| Gene Summary | This enzyme functions in the methionine salvage pathway by catalyzing the interconversion of methylthioribose-1-phosphate and methythioribulose-1-phosphate. Elevated expression of the encoded protein is associated with metastatic melanoma and this protein promotes melanoma cell invasion independent of its enzymatic activity. Mutations in this gene may be associated with vanishing white matter disease (VMWD). [provided by RefSeq, Jul 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC200993 | MRI1 (Myc-DDK-tagged)-Human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1 |
CNY 3,656.00 |
|
| RC200993L3 | Lenti ORF clone of Human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC200993L4 | Lenti ORF clone of Human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC319380 | MRI1 (untagged)-Human methylthioribose-1-phosphate isomerase homolog (S. cerevisiae) (MRI1), transcript variant 1 |
CNY 3,656.00 |
