CIAO2B (NM_016062) Human Tagged ORF Clone

CAT#: RG202825

  • TrueORF®

FAM96B (tGFP-tagged) - Human family with sequence similarity 96, member B (FAM96B), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_016062" in other vectors (6)

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CNY 2,800.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


CIAO2B Antibody - N-terminal region
    • 100 ul

CNY 4,628.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms CGI-128; CIA2B; FAM96B; MIP18
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202825 representing NM_016062
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAGGCGGCGGCGGGGTCGGCGGCGGCCTCCTGGAGAATGCCAACCCCCTCATCTACCAGCGCTCTG
GGGAGCGGCCTGTGACGGCAGGCGAGGAGGACGAGCAGGTTCCCGACAGCATCGACGCACGCGAGATCTT
CGATCTGATTCGCTCCATCAATGACCCGGAGCATCCACTGACGCTAGAGGAGTTGAACGTAGTAGAGCAG
GTGCGGGTTCAGGTTAGCGACCCCGAGAGTACAGTGGCTGTGGCTTTCACACCAACCATTCCGCACTGCA
GCATGGCCACCCTTATTGGTCTGTCCATCAAGGTCAAGCTTCTGCGCTCCCTTCCTCAGCGTTTCAAGAT
GGACGTGCACATTACTCCGGGGACCCATGCCTCAGAGCATGCAGTGAACAAGCAACTTGCAGATAAGGAG
CGGGTGGCAGCTGCCCTGGAGAACACCCACCTCTTGGAGGTTGTGAATCAGTGCCTGTCAGCCCGCTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202825 representing NM_016062
Red=Cloning site Green=Tags(s)

MVGGGGVGGGLLENANPLIYQRSGERPVTAGEEDEQVPDSIDAREIFDLIRSINDPEHPLTLEELNVVEQ
VRVQVSDPESTVAVAFTPTIPHCSMATLIGLSIKVKLLRSLPQRFKMDVHITPGTHASEHAVNKQLADKE
RVAAALENTHLLEVVNQCLSARS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016062
ORF Size 489 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_016062.4
RefSeq Size 670 bp
RefSeq ORF 492 bp
Locus ID 51647
UniProt ID Q9Y3D0
Gene Summary Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins (PubMed:23891004, PubMed:22678362, PubMed:22678361). As a CIA complex component and in collaboration with CIAO1 and MMS19, binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins (PubMed:23891004). As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD (PubMed:20797633).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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