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PPP2R1B (NM_181699) Human Tagged ORF Clone
CAT＃: RG205346
- TrueORF®
PPP2R1B (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2
ORF Plasmid: DDK
"NM_181699" in other vectors (6)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PP2A-Abeta; PR65B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205346 representing NM_181699
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCATCAGAGCTCGGGACCGGCCCAGGAGCAGCGGGTGGAGATGGAGATGATTCGCTATACC CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAAGACGTGCAGCTCCGACTCAACAGTATTAAGAAGTT ATCAACAATTGCCCTAGCACTTGGAGTAGAAAGGACCCGAAGTGAATTGTTGCCATTTCTTACAGATACA ATTTATGATGAAGATGAGGTACTATTAGCTCTTGCTGAGCAGCTGGGAAATTTCACTGGCCTAGTGGGAG GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAATCTGGCAACTGTGGAAGAGACTGTTGTTCG TGACAAGGCTGTGGAGTCCCTGAGACAGATCTCCCAGGAGCATACTCCTGTTGCTCTGGAAGCTTATTTT GTACCTCTGGTGAAACGCTTAGCAAGTGGGGATTGGTTCACCTCTCGCACATCTGCATGTGGTTTGTTCA GCGTTTGCTATCCCAGGGCATCAAATGCTGTTAAAGCAGAAATCAGACAGCAATTCCGTTCCTTGTGCTC AGATGACACACCAATGGTACGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA GACAGTGTGAAAAGTGAAATTGTTCCACTGTTCACTAGTCTAGCTTCAGATGAACAGGATTCAGTGCGCC TCCTTGCTGTGGAAGCTTGTGTCAGTATTGCCCAGTTATTGTCTCAGGATGACCTTGAGACTTTGGTGAT GCCTACACTTCGACAAGCAGCAGAAGATAAATCTTGGCGCGTTCGCTATATGGTGGCTGACAGATTTTCA GAGCTCCAGAAAGCCATGGGTCCTAAAATCACCCTAAATGACCTCATCCCCGCCTTTCAGAACCTACTTA AAGACTGTGAAGCTGAAGTCCGGGCTGCTGCTGCCCACAAAGTAAAAGAACTTGGTGAGAACTTGCCCAT TGAAGATAGAGAGACCATAATTATGAATCAAATTCTGCCTTATATAAAGGAATTAGTATCCGATACCAAT CAACATGTCAAATCGGCTCTAGCTTCTGTAATTATGGGATTGTCTACTATTTTGGGCAAAGAAAATACCA TTGAACATCTTCTACCTCTTTTCTTAGCTCAGTTAAAGGATGAGTGTCCTGACGTTCGTTTGAATATCAT CTCCAATTTGGATTGTGTAAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCTGCCATA GTGGAGCTGGCAGAAGATGCCAAATGGAGGGTCCGCCTGGCCATCATTGAGTATATGCCGCTGCTGGCAG GCCAGCTGGGTGTGGAATTCTTTGATGAAAAGCTGAATTCTTTATGTATGGCTTGGCTCGTGGACCATGT ATACGCCATCCGAGAAGCTGCCACCAACAACCTCATGAAACTAGTTCAGAAGTTTGGTACAGAGTGGGCC CAAAATACTATTGTTCCCAAAGTGTTAGTAATGGCAAATGATCCTAATTACTTGCATAGAATGACCACTT TATTCTGCATTAATGCACTGTCTGAGGCCTGTGGTCAGGAAATAACTACTAAGCAAATGCTGCCCATCGT ATTAAAAATGGCAGGAGACCAAGTAGCAAATGTTCGCTTCAATGTGGCCAAATCTCTACAAAAGATTGGA CCAATTCTAGATACCAATGCTTTACAGGGAGAAGTGAAGCCAGTACTACAGAAGTTAGGTCAAGATGAAG ACATGGATGTCAAATACTTTGCACAGGAAGCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGAATT TCCTGTGAAGGACAGTGGAGAACCCAGTGTCCCTCGGGCTGACAAGAACCACTTCCCAAGACCCACAGTG CCTGGAGAGGACATGGGGAAGGGACCAGTGTATCAGTTGCGTGGAGATACTAGAGACACACTTGCCCAGC TGGGAATTGCAGAGCTAGTGCATTTCTCCCAAAGCACAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205346 representing NM_181699
Red=Cloning site Green=Tags(s) MAGASELGTGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDT IYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLENLATVEETVVRDKAVESLRQISQEHTPVALEAYF VPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQQFRSLCSDDTPMVRRAAASKLGEFAKVLEL DSVKSEIVPLFTSLASDEQDSVRLLAVEACVSIAQLLSQDDLETLVMPTLRQAAEDKSWRVRYMVADRFS ELQKAMGPKITLNDLIPAFQNLLKDCEAEVRAAAAHKVKELGENLPIEDRETIIMNQILPYIKELVSDTN QHVKSALASVIMGLSTILGKENTIEHLLPLFLAQLKDECPDVRLNIISNLDCVNEVIGIRQLSQSLLPAI VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWA QNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGQEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIG PILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLEFPVKDSGEPSVPRADKNHFPRPTV PGEDMGKGPVYQLRGDTRDTLAQLGIAELVHFSQSTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181699 |
| ORF Size | 2001 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181699.1, NP_859050.1 |
| RefSeq Size | 2139 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 5519 |
| UniProt ID | P30154 |
| Protein Families | Druggable Genome, Phosphatase, Transcription Factors |
| Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
| Gene Summary | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205346 | PPP2R1B (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2 |
CNY 3,990.00 |
|
| RC205346L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205346L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC205346L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205346L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC123371 | PPP2R1B (untagged)-Human protein phosphatase 2, regulatory subunit A, beta (PPP2R1B), transcript variant 2 |
CNY 2,950.00 |
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