SIRT7 (NM_016538) Human Tagged ORF Clone
CAT＃: RG205658
- TrueORF®
SIRT7 (tGFP-tagged) - Human sirtuin 7 (SIRT7)
ORF Plasmid: DDK
"NM_016538" in other vectors (6)
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CNY 7088.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SIR2L7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205658 representing NM_016538
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGGGGGTCTGAGCCGCTCCGAGCGCAAAGCGGCGGAGCGGGTCCGGAGGTTGCGGGAGGAGC AGCAGAGGGAGCGCCTCCGCCAGGTGTCGCGCATCCTGAGGAAGGCGGCGGCGGAGCGCAGCGCCGAGGA GGGCCGGCTGCTGGCCGAGAGCGCGGACCTGGTAACGGAGCTGCAGGGCCGGAGCCGGCGGCGCGAGGGC CTGAAGCGGCGGCAGGAGGAGGTGTGCGACGACCCGGAGGAGCTGCGGGGGAAGGTCCGGGAGCTGGCCA GCGCCGTCCGGAACGCCAAATACTTGGTCGTCTACACAGGCGCGGGAATCAGCACGGCAGCGTCTATCCC AGACTACCGGGGCCCTAATGGAGTGTGGACACTGCTTCAGAAAGGGAGAAGCGTTAGTGCTGCCGACCTG AGCGAGGCCGAGCCAACCCTCACCCACATGAGCATCACCCGTCTGCATGAGCAGAAGCTGGTGCAGCATG TGGTGTCTCAGAACTGTGACGGGCTCCACCTGAGGAGTGGGCTGCCGCGCACGGCCATCTCCGAGCTCCA CGGGAACATGTACATTGAAGTCTGTACCTCCTGCGTTCCCAACAGGGAGTACGTGCGGGTGTTCGATGTG ACGGAGCGCACTGCCCTCCACAGACACCAGACAGGCCGGACCTGCCACAAGTGTGGGACCCAGCTGCGGG ACACCATTGTGCACTTTGGGGAGAGGGGGACGTTGGGGCAGCCTCTGAACTGGGAAGCGGCGACCGAGGC TGCCAGCAGAGCAGACACCATCCTGTGTCTAGGGTCCAGCCTGAAGGTTCTAAAGAAGTACCCACGCCTC TGGTGCATGACCAAGCCCCCTAGCCGGCGGCCGAAGCTTTACATCGTGAACCTGCAGTGGACCCCGAAGG ATGACTGGGCTGCCCTGAAGCTACATGGGAAGTGTGATGACGTCATGCGGCTCCTCATGGCCGAGCTGGG CTTGGAGATCCCCGCCTATAGCAGGTGGCAGGATCCCATTTTCTCACTGGCGACTCCCCTGCGTGCTGGT GAAGAAGGCAGCCACAGTCGGAAGTCGCTGTGCAGAAGCAGAGAGGAGGCCCCGCCTGGGGACCGGGGTG CACCGCTTAGCTCGGCCCCCATCCTAGGGGGCTGGTTTGGCAGGGGCTGCACAAAACGCACAAAAAGGAA GAAAGTGACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205658 representing NM_016538
Red=Cloning site Green=Tags(s) MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREG LKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADL SEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDV TERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRL WCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAG EEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016538 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016538.1, NP_057622.1 |
| RefSeq Size | 1718 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 51547 |
| UniProt ID | Q9NRC8 |
| Domains | SIR2 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SirT7 auto-ADP-ribosylation regulates glucose starvation response through mH2A1
,Simonet, N;Thackray, J;Vazquez, B;Ianni, A;Espinosa-Alcantud, M;Morales-Sanfrutos, J;Hurtado-Bagès, S;Sabidó, E;Buschbeck, M;Tischfield, J;De La Torre, C;Esteller, M;Braun, T;Olivella, M;Serrano, L;Vaquero, A;,
Sci. Adv.
,PubMed ID 32832656
[SIRT7]
|
|
Sirtuin 7 is Decreased in Pulmonary Fibrosis and Regulates the Fibrotic Phenotype of Lung Fibroblasts
,Wyman, AE;Noor, Z;Fishelevich, R;Lockatell, V;Shah, NG;Todd, NW;Atamas, SP;,
Am. J. Physiol. Lung Cell Mol. Physiol.
,PubMed ID 28385812
[SIRT7]
|
|
SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair
,Vazquez, BN;Thackray, JK;Simonet, NG;Kane-Goldsmith, N;Martinez-Redondo, P;Nguyen, T;Bunting, S;Vaquero, A;Tischfield, JA;Serrano, L;,
EMBO J.
,PubMed ID 27225932
[SIRT7]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205658 | SIRT7 (Myc-DDK-tagged)-Human sirtuin 7 (SIRT7) |
CNY 5488.00 |
|
| RC205658L1 | Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged |
CNY 7888.00 |
|
| RC205658L2 | Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged |
CNY 5890.00 |
|
| RC205658L3 | Lenti ORF clone of Human sirtuin 7 (SIRT7), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205658L4 | Lenti ORF clone of Human sirtuin 7 (SIRT7), mGFP tagged |
CNY 5890.00 |
|
| SC319613 | SIRT7 (untagged)-Human sirtuin 7 (SIRT7) |
CNY 5488.00 |
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
Mouse monoclonal turboGFP antibody, clone OTI2H8
