United States
Germany
Japan
United Kingdom
China
ROCK1 (NM_005406) Human Tagged ORF Clone
CAT＃: RG211547
- TrueORF®
ROCK1 (tGFP-tagged) - Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)
ORF Plasmid: DDK
"NM_005406" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 14,820.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | P160ROCK; ROCK-I |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211547 representing NM_005406
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACTGGGGACAGTTTTGAGACTCGATTTGAAAAAATGGACAACCTGCTGCGGGATCCCAAATCGG AAGTGAATTCGGATTGTTTGCTGGATGGATTGGATGCTTTGGTATATGATTTGGATTTTCCTGCCTTAAG AAAAAACAAAAATATTGACAACTTTTTAAGCAGATATAAAGACACAATAAATAAAATCAGAGATTTACGA ATGAAAGCTGAAGATTATGAAGTAGTGAAGGTGATTGGTAGAGGTGCATTTGGAGAAGTTCAATTGGTAA GGCATAAATCCACCAGGAAGGTATATGCTATGAAGCTTCTCAGCAAATTTGAAATGATAAAGAGATCTGA TTCTGCTTTTTTCTGGGAAGAAAGGGACATCATGGCTTTTGCCAACAGTCCTTGGGTTGTTCAGCTTTTT TATGCATTCCAAGATGATCGTTATCTCTACATGGTGATGGAATACATGCCTGGTGGAGATCTTGTAAACT TAATGAGCAACTATGATGTGCCTGAAAAATGGGCACGATTCTATACTGCAGAAGTAGTTCTTGCATTGGA TGCAATCCATTCCATGGGTTTTATTCACAGAGATGTGAAGCCTGATAACATGCTGCTGGATAAATCTGGA CATTTGAAGTTAGCAGATTTTGGTACTTGTATGAAGATGAATAAGGAAGGCATGGTACGATGTGATACAG CGGTTGGAACACCTGATTATATTTCCCCTGAAGTATTAAAATCCCAAGGTGGTGATGGTTATTATGGAAG AGAATGTGACTGGTGGTCGGTTGGGGTATTTTTATACGAAATGCTTGTAGGTGATACACCTTTTTATGCA GATTCTTTGGTTGGAACTTACAGTAAAATTATGAACCATAAAAATTCACTTACCTTTCCTGATGATAATG ACATATCAAAAGAAGCAAAAAACCTTATTTGTGCCTTCCTTACTGACAGGGAAGTGAGGTTAGGGCGAAA TGGTGTAGAAGAAATCAAACGACATCTCTTCTTCAAAAATGACCAGTGGGCTTGGGAAACGCTCCGAGAC ACTGTAGCACCAGTTGTACCCGATTTAAGTAGTGACATTGATACTAGTAATTTTGATGACTTGGAAGAAG ATAAAGGAGAGGAAGAAACATTCCCTATTCCTAAAGCTTTCGTTGGCAATCAACTACCTTTTGTAGGATT TACATATTATAGCAATCGTAGATACTTATCTTCAGCAAATCCTAATGATAACAGAACTAGCTCCAATGCA GATAAAAGCTTGCAGGAAAGTTTGCAAAAAACAATCTATAAGCTGGAAGAACAGCTGCATAATGAAATGC AGTTAAAAGATGAAATGGAGCAGAAGTGCAGAACCTCAAACATAAAACTAGACAAGATAATGAAAGAATT GGATGAAGAGGGAAATCAAAGAAGAAATCTAGAATCTACAGTGTCTCAGATTGAGAAGGAGAAAATGTTG CTACAGCATAGAATTAATGAGTACCAAAGAAAAGCTGAACAGGAAAATGAGAAGAGAAGAAATGTAGAAA ATGAAGTTTCTACATTAAAGGATCAGTTGGAAGACTTAAAGAAAGTCAGTCAGAATTCACAGCTTGCTAA TGAGAAGCTGTCCCAGTTACAAAAGCAGCTAGAAGAAGCCAATGACTTACTTAGGACAGAATCGGACACA GCTGTAAGATTGAGGAAGAGTCACACAGAGATGAGCAAGTCAATTAGTCAGTTAGAGTCCCTGAACAGAG AGTTGCAAGAGAGAAATCGAATTTTAGAGAATTCTAAGTCACAAACAGACAAAGATTATTACCAGCTGCA AGCTATATTAGAAGCTGAACGAAGAGACAGAGGTCATGATTCTGAGATGATTGGAGACCTTCAAGCTCGA ATTACATCTTTACAAGAGGAGGTGAAGCATCTCAAACATAATCTCGAAAAAGTGGAAGGAGAAAGAAAAG AGGCTCAAGACATGCTTAATCACTCAGAAAAGGAAAAGAATAATTTAGAGATAGATTTAAACTACAAACT TAAATCATTACAACAACGGTTAGAACAAGAGGTAAATGAACACAAAGTAACCAAAGCTCGTTTAACTGAC AAACATCAATCTATTGAAGAGGCAAAGTCTGTGGCAATGTGTGAGATGGAAAAAAAGCTGAAAGAAGAAA GAGAAGCTCGAGAGAAGGCTGAAAATCGGGTTGTTCAGATTGAGAAACAGTGTTCCATGCTAGACGTTGA TCTGAAGCAATCTCAGCAGAAACTAGAACATTTGACTGGAAATAAAGAAAGGATGGAGGATGAAGTTAAG AATCTAACCCTGCAACTGGAGCAGGAATCAAATAAGCGGCTGTTGTTACAAAATGAATTGAAGACTCAAG CATTTGAGGCAGACAATTTAAAAGGTTTAGAAAAGCAGATGAAACAGGAAATAAATACTTTATTGGAAGC AAAGAGATTATTAGAATTTGAGTTAGCTCAGCTTACGAAACAGTATAGAGGAAATGAAGGACAGATGCGG GAGCTACAAGATCAGCTTGAAGCTGAGCAATATTTCTCGACACTTTATAAAACCCAGGTAAAGGAACTTA AAGAAGAAATTGAAGAAAAAAACAGAGAAAATTTAAAGAAAATACAGGAACTACAAAATGAAAAAGAAAC TCTTGCTACTCAGTTGGATCTAGCAGAAACAAAAGCTGAGTCTGAGCAGTTGGCGCGAGGCCTTCTGGAA GAACAGTATTTTGAATTGACGCAAGAAAGCAAGAAAGCTGCTTCAAGAAATAGACAAGAGATTACAGATA AAGATCACACTGTTAGTCGGCTTGAAGAAGCAAACAGCATGCTAACCAAAGATATTGAAATATTAAGAAG AGAGAATGAAGAGCTAACAGAGAAAATGAAGAAGGCAGAGGAAGAATATAAACTGGAGAAGGAGGAGGAG ATCAGTAATCTTAAGGCTGCCTTTGAAAAGAATATCAACACTGAACGAACCCTTAAAACACAGGCTGTTA ACAAATTGGCAGAAATAATGAATCGAAAAGATTTTAAAATTGATAGAAAGAAAGCTAATACACAAGATTT GAGAAAGAAAGAAAAGGAAAATCGAAAGCTGCAACTGGAACTCAACCAAGAAAGAGAGAAATTCAACCAG ATGGTAGTGAAACATCAGAAGGAACTGAATGACATGCAAGCGCAATTGGTAGAAGAATGTGCACATAGGA ATGAGCTTCAGATGCAGTTGGCCAGCAAAGAGAGTGATATTGAGCAATTGCGTGCTAAACTTTTGGACCT CTCGGATTCTACAAGTGTTGCTAGTTTTCCTAGTGCTGATGAAACTGATGGTAACCTCCCAGAGTCAAGA ATTGAAGGTTGGCTTTCAGTACCAAATAGAGGAAATATCAAACGATATGGCTGGAAGAAACAGTATGTTG TGGTAAGCAGCAAAAAAATTTTGTTCTATAATGACGAACAAGATAAGGAGCAATCCAATCCATCTATGGT ATTGGACATAGATAAACTGTTTCACGTTAGACCTGTAACCCAAGGAGATGTGTATAGAGCTGAAACTGAA GAAATTCCTAAAATATTCCAGATACTATATGCAAATGAAGGTGAATGTAGAAAAGATGTAGAGATGGAAC CAGTACAACAAGCTGAAAAAACTAATTTCCAAAATCACAAAGGCCATGAGTTTATTCCTACACTCTACCA CTTTCCTGCCAATTGTGATGCCTGTGCCAAACCTCTCTGGCATGTTTTTAAGCCACCCCCTGCCCTAGAG TGTCGAAGATGCCATGTTAAGTGCCACAGAGATCACTTAGATAAGAAAGAGGACTTAATTTGTCCATGTA AAGTAAGTTATGATGTAACATCAGCAAGAGATATGCTGCTGTTAGCATGTTCTCAGGATGAACAAAAAAA ATGGGTAACTCATTTAGTAAAGAAAATCCCTAAGAATCCACCATCTGGTTTTGTTCGTGCTTCCCCTCGA ACGCTTTCTACAAGATCCACTGCAAATCAGTCTTTCCGGAAAGTGGTCAAAAATACATCTGGAAAAACTA GT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211547 representing NM_005406
Red=Cloning site Green=Tags(s) MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLR MKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLF YAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSG HLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYA DSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRD TVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNA DKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKML LQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDT AVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQAR ITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTD KHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVK NLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMR ELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLE EQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEE ISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQ MVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESR IEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETE EIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALE CRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPR TLSTRSTANQSFRKVVKNTSGKTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005406 |
| ORF Size | 4062 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005406.3 |
| RefSeq Size | 6650 bp |
| RefSeq ORF | 4065 bp |
| Locus ID | 6093 |
| UniProt ID | Q13464 |
| Domains | pkinase, HR1, PH |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway |
| Gene Summary | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211547 | ROCK1 (Myc-DDK-tagged)-Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) |
CNY 14,824.00 |
|
| RC211547L1 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), Myc-DDK-tagged |
CNY 15,390.00 |
|
| RC211547L2 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mGFP tagged |
CNY 15,390.00 |
|
| RC211547L3 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), Myc-DDK-tagged |
CNY 15,390.00 |
|
| RC211547L4 | Lenti ORF clone of Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1), mGFP tagged |
CNY 15,390.00 |
|
| SC124118 | ROCK1 (untagged)-Human Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) |
CNY 13,904.00 |
