KLHL15 (NM_030624) Human Tagged ORF Clone

CAT＃: RG213520

TrueORF^®

KLHL15 (tGFP-tagged) - Human kelch-like 15 (Drosophila) (KLHL15)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_030624" in other vectors (6)

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CNY 5,040.00

货期*

4周

规格

Cited in 1 publication.

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA306799

Rabbit Polyclonal KLHL15 Antibody

CNY 5,808.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	HEL-S-305
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG213520 representing NM_030624 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGGGACGTGGAAGGATTCTGTTCCTCCATCCACGACACCAGTGTCTCTGCTGGGTTCAGAGCAC TGTATGAGGAGGGATTGCTTCTTGATGTCACTCTGGTTATTGAAGATCATCAGTTCCAGGCCCATAAAGC ACTCTTGGCCACCCAGAGTGATTACTTCAGAATTATGTTTACTGCAGACATGAGGGAACGAGATCAGGAC AAAATTCATTTAAAAGGTCTAACAGCTACCGGTTTCAGCCATGTCCTGCAATTTATGTACTATGGAACTA TAGAGCTGAGTATGAATACCGTTCATGAGATTCTTCAGGCTGCCATGTATGTTCAACTTATAGAAGTGGT GAAGTTCTGCTGCTCTTTTCTCTTAGCGAAGATCTGCCTAGAAAATTGTGCAGAAATTATGAGACTCTTA GATGATTTCGGCGTAAACATCGAGGGAGTCAGGGAGAAGTTAGACACCTTTCTGCTAGACAACTTTGTGC CACTCATGTCTAGGCCTGACTTTCTGTCCTATCTGAGCTTTGAGAAGCTCATGTCTTACTTGGATAATGA TCATCTGAGCAGGTTCCCAGAGATAGAGCTGTACGAGGCTGTGCAGTCTTGGCTGCGGCATGATAGAAGA CGCTGGAGACATACCGATACCATCATTCAGAATATCCGGTTTTGCTTGATGACCCCAACCAGCGTTTTTG AGAAGGTTAAGACATCAGAATTTTATAGATACTCCCGACAGCTCCGTTACGAAGTTGACCAAGCATTGAA TTACTTTCAGAATGTTCACCAGCAGCCTTTGTTGGATATGAAGTCAAGCCGCATCCGTTCTGCAAAACCG CAAACTACAGTATTTCGAGGAATGATTGGACATAGCATGGTTAACAGTGAAATACTTCTCTTAAAGAAAC CAAGAGTCTGGTGGGAGCTAGAAGGCCCACAAGTACCTCTGCGACCTGACTGCCTTGCTATCGTCAATAA TTTTGTGTTCCTGTTAGGCGGGGAAGAGCTGGGCCCGGATGGTGAATTCCATGCTTCTTCCAAAGTATTC AGGTATGACCCGAGACAGAACTCCTGGCTGCAGATGGCAGATATGTCTGTACCACGCTCTGAATTTGCTG TAGGTGTTATTGGGAAGTTTATTTACGCCGTAGCAGGCAGAACCAGAGATGAGACTTTCTATTCAACTGA GAGATATGACATCACCAACGATAAATGGGAATTTGTGGATCCTTATCCAGTTAACAAATATGGACATGAG GGGACAGTGCTCAATAACAAATTGTTTATCACCGGTGGAATCACCTCATCTTCCACCTCCAAGCAAGTGT GCGTGTTTGACCCCAGCAAAGAAGGGACCATAGAACAACGGACCAGGAGAACTCAAGTGGTTACCAACTG TTGGGAGAATAAGAGCAAGATGAATTACGCGAGATGCTTTCACAAGATGATTTCTTACAATGGCAAGCTT TATGTCTTCGGTGGTGTCTGTGTGATCTTGAGGGCCTCTTTCGAATCTCAGGGATGCCCTTCTACAGAAG TATACAACCCAGAGACTGATCAGTGGACCATCTTGGCATCCATGCCGATTGGTAGAAGTGGCCATGGTGT GACTGTGCTGGACAAACAAATAATGGTTCTTGGAGGCCTTTGTTATAATGGTCATTACAGCGATTCCATC CTCACTTTTGATCCGGATGAAAACAAGTGGAAGGAAGATGAGTACCCTCGGATGCCCTGCAAGCTGGATG GTTTACAAGTATGCAACCTGCATTTTCCGGACTATGTACTGGATGAGGTCAGGCGTTGCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG213520 representing NM_030624 Red=Cloning site Green=Tags(s) MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQD KIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLL DDFGVNIEGVREKLDTFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRR RWRHTDTIIQNIRFCLMTPTSVFEKVKTSEFYRYSRQLRYEVDQALNYFQNVHQQPLLDMKSSRIRSAKP QTTVFRGMIGHSMVNSEILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVF RYDPRQNSWLQMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHE GTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCFHKMISYNGKL YVFGGVCVILRASFESQGCPSTEVYNPETDQWTILASMPIGRSGHGVTVLDKQIMVLGGLCYNGHYSDSI LTFDPDENKWKEDEYPRMPCKLDGLQVCNLHFPDYVLDEVRRCN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_030624
ORF Size	1812 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_030624.1, NP_085127.1
RefSeq Size	2146 bp
RefSeq ORF	1815 bp
Locus ID	80311
UniProt ID	Q96M94
Gene Summary	This gene encodes a member of the kelch-like family of proteins that share a common domain structure consisting of an N-terminal broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger domain and C-terminal kelch repeat motifs. The encoded protein may be involved in protein ubiquitination and cytoskeletal organization. [provided by RefSeq, Apr 2009]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection ,null, Nature Communications ,PubMed ID 27561354 [KLHL15]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC213520	KLHL15 (Myc-DDK-tagged)-Human kelch-like 15 (Drosophila) (KLHL15)	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,504.00 CNY 4,560.00
RC213520L1	Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC213520L2	Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC213520L3	Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC213520L4	Lenti ORF clone of Human kelch-like 15 (Drosophila) (KLHL15), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
SC305271	KLHL15 (untagged)-Human kelch-like 15 (Drosophila) (KLHL15)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,790.00

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