United States
Germany
Japan
United Kingdom
China
WIPI2 (NM_001033520) Human Tagged ORF Clone
CAT＃: RG216506
- TrueORF®
WIPI2 (tGFP-tagged) - Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5
ORF Plasmid: DDK
"NM_001033520" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ATG18B; Atg21; CGI-50; IDDSSA; WIPI-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216506 representing NM_001033520
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCACGCATGTACTTCCCTTTGTGATTTCAGCCGATACGGAAGATGTGTGCATTGTAGAGAGATTGT TCTCCAGCAGCCTAGTGGCCATCGTCAGCCTTAAAGCACCAAGGAAGCTAAAGGTTTGCCACTTTAAGAA GGGAACTGAGATCTGCAACTACAGCTACTCCAACACGATTCTGGCTGTGAAGCTCAACAGGCAGAGGCTG ATAGTATGCCTGGAGGAGTCCCTGTACATCCACAACATTCGGGACATGAAGGTGCTGCATACGATCAGGG AGACGCCTCCAAACCCTGCAGGCCTGTGTGCGCTGTCAATCAACAACGACAACTGCTACTTGGCGTACCC AGGGAGCGCGACCATCGGAGAGGTGCAGGTCTTCGATACCATTAATTTGAGAGCTGCAAACATGATTCCG GCTCACGACAGTCCTTTAGCGGCACTGGCCTTTGACGCAAGTGGAACTAAACTTGCCACGGCTTCGGAGA AGGGGACCGTGATTAGGGTATTTTCCATTCCAGAAGGACAAAAACTCTTTGAGTTTCGGAGAGGAGTAAA GAGGTGCGTGAGCATCTGCTCCCTGGCCTTCAGCATGGACGGCATGTTCCTCTCCGCCTCCAGCAACACT GAGACCGTGCACATCTTCAAACTCGAGACTGTGAAAGAAAAACCCCCAGAGGAGCCCACCACCTGGACCG GGTACTTCGGGAAAGTGCTCATGGCCTCCACCAGCTACCTGCCTTCCCAAGTGACAGAAATGTTCAACCA GGGCAGAGCCTTCGCCACGGTCCGCCTGCCATTCTGCGGCCACAAAAACATCTGCTCGCTAGCCACAATT CAGAAGATCCCGCGGTTGTTGGTGGGTGCCGCCGACGGGTACCTGTACATGTACAACCTGGACCCCCAGG AGGGCGGCGAGTGTGCCCTGATGAAGCAGCACCGGCTGGACGGCAGTCTGGAAACGACCAATGAGATCTT GGACTCTGCCTCTCACGACTGCCCCTTAGTCACTCAGACATACGGCGCAGCTGCAGGAAAAGCCTACACA GACGACCTGGGTGCTGTGGGTGGCGCCTGCCTGGAGGACGAGGCCAGCGCCCTGCGCCTGGATGAGGACA GCGAGCACCCGCCCATGATTCTTCGGACTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216506 representing NM_001033520
Red=Cloning site Green=Tags(s) MFTHVLPFVISADTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRL IVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIP AHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNT ETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATI QKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKAYT DDLGAVGGACLEDEASALRLDEDSEHPPMILRTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033520 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033520.1, NP_001028692.1 |
| RefSeq Size | 4295 bp |
| RefSeq ORF | 1155 bp |
| Locus ID | 26100 |
| UniProt ID | Q9Y4P8 |
| Gene Summary | WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216506 | WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 |
CNY 3,656.00 |
|
| RC216506L3 | Lenti-ORF clone of WIPI2 (Myc-DDK-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 |
CNY 5,890.00 |
|
| RC216506L4 | Lenti-ORF clone of WIPI2 (mGFP-tagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 |
CNY 5,890.00 |
|
| SC302717 | WIPI2 (untagged)-Human WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5 |
CNY 6,270.00 |
