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MYO5A (NM_000259) Human Tagged ORF Clone
CAT＃: RG218817
- TrueORF®
MYO5A (tGFP-tagged) - Human myosin VA (heavy chain 12, myoxin) (MYO5A), transcript variant 1
ORF Plasmid: DDK
"NM_000259" in other vectors (2)
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CNY 20,330.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GS1; MYH12; MYO5; MYR12 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218817 representing NM_000259
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGTCGGAGCTCTACACAAAGTTTGCCAGGGTTTGGATACCTGATCCAGAGGAAGTCTGGAAGT CAGCAGAGCTGCTCAAAGATTATAAGCCAGGAGATAAAGTCCTCCTGCTTCACCTCGAGGAAGGAAAGGA TTTGGAATACCATCTAGATCCAAAGACCAAGGAGCTGCCTCACTTACGAAATCCTGACATACTTGTTGGT GAAAATGACCTCACAGCCCTCAGCTATCTTCATGAGCCTGCTGTGCTCCATAATCTCAGAGTCCGCTTTA TTGATTCCAAACTTATTTATACGTATTGTGGTATAGTCCTAGTAGCTATAAATCCCTATGAACAGCTGCC TATTTATGGAGAAGATATTATTAATGCATACAGTGGTCAGAACATGGGTGATATGGATCCACATATCTTT GCAGTAGCTGAAGAAGCTTACAAGCAAATGGCCAGAGATGAACGAAATCAGTCCATCATCGTAAGTGGAG AGTCTGGGGCAGGAAAAACAGTCTCAGCTAAGTATGCCATGCGATACTTTGCAACTGTGAGTGGTTCTGC CAGTGAGGCCAATGTGGAGGAAAAGGTCTTGACCTCCAACCCCATCATGGAGTCCATTGGAAATGCTAAA ACAACCAGGAATGATAATAGCAGCCGTTTTGGGAAGTATATTGAGATTGGTTTTGATAAGAGATATCGAA TCATTGGTGCCAATATGAGAACTTATCTTTTAGAGAAATCCAGAGTGGTATTCCAGGCAGAAGAGGAGAG AAACTATCATATCTTCTATCAGCTTTGTGCCTCAGCAAAGTTACCTGAATTTAAAATGCTACGATTAGGA AATGCAGATAACTTTAATTACACAAAACAAGGAGGCAGTCCTGTGATTGAAGGAGTGGATGATGCAAAGG AGATGGCACATACTAGGCAGGCCTGCACTTTGCTAGGAATTAGTGAATCTCATCAAATGGGAATTTTCCG AATACTTGCTGGCATCCTTCACTTAGGCAATGTTGGATTTACATCCCGAGATGCAGACAGCTGCACAATA CCTCCCAAGCATGAACCTCTCTGCATCTTCTGTGACCTCATGGGTGTGGACTATGAGGAGATGTGTCACT GGCTCTGCCATCGGAAACTGGCTACTGCCACAGAGACATACATCAAGCCCATCTCCAAGCTGCAGGCCAC GAATGCCCGCGATGCTTTGGCCAAACACATCTATGCCAAGCTCTTTAACTGGATTGTAGATAATGTCAAT CAGGCTCTCCATTCTGCTGTCAAACAGCACTCTTTTATTGGTGTGCTAGACATTTACGGATTTGAAACAT TTGAGATAAATAGTTTTGAACAGTTTTGCATAAATTATGCAAATGAAAAACTACAGCAACAATTCAATAT GCATGTCTTCAAATTGGAGCAAGAAGAATATATGAAGGAACAAATTCCATGGACACTCATAGATTTTTAT GATAATCAGCCTTGTATTAATCTTATAGAATCAAAACTAGGCATTCTAGATTTACTGGATGAGGAATGCA AGATGCCTAAAGGCACAGATGACACCTGGGCCCAAAAATTGTACAACACACATTTGAACAAATGTGCACT CTTTGAAAAGCCTCGTCTATCAAACAAAGCTTTCATCATCCAACATTTTGCTGACAAAGTGGAATACCAG TGTGAAGGATTTCTCGAAAAGAATAAAGACACCGTTTTTGAAGAACAAATTAAAGTTCTTAAATCAAGCA AGTTTAAGATGCTACCAGAACTATTTCAAGATGATGAGAAGGCCATCAGTCCAACTTCAGCCACCTCCTC AGGGCGCACACCCCTCACACGAACTCCTGCAAAGCCCACCAAAGGCAGACCAGGCCAAATGGCCAAAGAG CACAAGAAAACAGTGGGGCATCAGTTCAGAAACTCCCTGCACCTGCTTATGGAGACACTCAATGCCACTA CCCCTCACTATGTGCGCTGTATCAAGCCTAATGACTTCAAGCTCCCATTCACGTTTGATGAGAAGAGGGC AGTGCAGCAGCTGAGAGCATGTGGTGTCCTGGAAACCATCCGAATCAGTGCGGCCGGTTTCCCCTCACGG TGGACTTACCAAGAATTTTTCAGCCGCTACCGTGTCCTAATGAAGCAGAAAGATGTGCTGAGTGACAGAA AGCAAACATGCAAGAATGTGTTAGAGAAACTGATACTGGACAAGGACAAATACCAGTTTGGTAAGACAAA GATCTTTTTCCGTGCCGGTCAAGTGGCCTATCTAGAAAAATTGAGAGCTGACAAACTGAGAGCTGCCTGC ATCCGGATCCAGAAGACCATCCGAGGGTGGCTGCTGAGAAAGAAGTACCTACGCATGCGGAAGGCAGCCA TCACCATGCAGAGATACGTGCGGGGCTACCAGGCCCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC AGCAACCATCATTCAAAAGTACTGGCGCATGTATGTGGTCCGCAGGAGGTACAAGATTAGACGAGCTGCC ACTATTGTTCTTCAGTCTTACTTGCGAGGCTTCTTGGCCAGAAATAGGTATCGCAAGATACTCCGTGGGC ACAAAGCAGTCATCATTCAGAAGCGAGTCCGGGGCTGGCTGGCCCGCACACACTACAAGAGGAGCATGCA TGCCATCATCTACCTTCAGTGCTGCTTCAGGCGGATGATGGCCAAGCGTGAGCTAAAGAAGCTCAAAATC GAGGCTCGCTCAGTGGAGCGCTATAAGAAGCTGCGCATCGGCATGGAGAACAAGATCATGCAGCTGCAGC GCAAAGTTGATGAGCAGAACAAAGACTACAAATGCCTTGTGGAGAAACTAACCAATCTGGAAGGAATATA CAACTCTGAGACTGAGAAACTACGAAGTGACTTAGAACGTCTTCAACTAAGTGAAGAGGAAGCGAAAGTT GCCACTGGGCGGGTCCTTAGTCTGCAGGAGGAAATTGCCAAGCTCCGGAAAGACCTGGAGCAAACTCGTT CAGAGAAAAAATGCATTGAGGAACATGCAGATCGATACAAACAAGAAACAGAGCAGCTGGTATCAAATCT GAAGGAAGAAAATACTTTGCTGAAGCAAGAAAAAGAAGCCCTCAATCACCGCATCGTGCAGCAGGCTAAG GAGATGACAGAAACTATGGAGAAGAAGTTAGTAGAAGAAACGAAACAACTGGAACTCGACCTTAATGATG AAAGGCTGAGATATCAGAACCTTCTGAATGAGTTCAGTCGCCTGGAAGAAAGATATGATGACCTCAAGGA AGAGATGACCCTTATGGTGCATGTGCCTAAGCCTGGACACAAGAGAACAGACTCCACCCACAGCAGCAAC GAGTCTGAATATATCTTTAGCTCTGAAATTGCAGAAATGGAAGACATTCCATCAAGGACAGAGGAACCAA GTGAGAAGAAGGTACCTCTGGACATGTCATTGTTCCTTAAGCTCCAGAAGCGGGTCACAGAGCTGGAGCA GGAGAAGCAGGTGATGCAGGATGAGCTGGACCGCAAGGAGGAGCAGGTGCTCCGCAGCAAGGCCAAGGAA GAAGAAAGACCACAAATTAGAGGTGCAGAACTGGAATATGAGTCACTCAAGCGTCAAGAACTAGAATCAG AAAACAAAAAACTGAAGAATGAGCTAAATGAGTTGCGCAAGGCCCTCAGTGAGAAAAGTGCCCCAGAGGT GACCGCCCCAGGTGCACCTGCCTACCGTGTCCTCATGGAGCAGCTGACCTCTGTGAGCGAGGAGCTTGAT GTCCGCAAGGAGGAAGTCCTCATCTTAAGGTCTCAACTGGTGAGCCAGAAAGAGGCCATCCAACCCAAGG ATGACAAGAATACAATGACAGATTCCACAATACTTTTGGAAGATGTACAAAAAATGAAAGATAAAGGTGA AATAGCACAAGCATACATTGGTTTGAAAGAAACAAATAGATCATCTGCTCTGGATTACCATGAGTTGAAT GAGGATGGAGAGCTGTGGCTGGTTTATGAAGGGTTAAAACAAGCCAATAGGCTCCTGGAATCCCAGCTGC AGTCACAGAAGAGGAGCCATGAGAATGAGGCCGAGGCCCTCCGTGGGGAGATCCAGAGCCTGAAGGAGGA GAACAACCGACAGCAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCAGAGGCCCGCATTGAGGCCAGC CTGCAGCACGAGATCACCCGGCTGACCAACGAAAACTTGGATTTGATGGAACAACTTGAAAAACAGGATA AGACGGTCCGTAAACTGAAAAAACAACTGAAAGTATTTGCCAAAAAAATTGGCGAACTAGAAGTGGGCCA GATGGAGAACATATCCCCAGGACAGATCATTGATGAACCCATCCGACCAGTCAACATTCCCAGGAAAGAA AAGGATTTCCAAGGGATGCTGGAATACAAGAAGGAGGATGAGCAAAAACTTGTTAAGAACCTGATTCTGG AACTGAAGCCACGTGGTGTAGCAGTCAATTTGATTCCAGGATTACCGGCATATATCCTGTTCATGTGTGT TCGACATGCTGACTACCTGAATGATGATCAGAAAGTAAGGTCGTTGCTAACATCAACAATTAACAGCATC AAAAAAGTATTGAAGAAAAGAGGTGATGATTTTGAAACCGTCTCCTTCTGGCTCTCTAACACATGCCGAT TTTTGCACTGCTTGAAACAGTACAGTGGAGAAGAGGGCTTTATGAAGCACAACACATCTCGCCAGAATGA ACACTGCCTCACCAATTTTGACCTGGCTGAGTATCGGCAGGTGCTGAGTGACTTGGCCATTCAGATCTAC CAGCAGCTCGTGCGGGTGTTAGAGAACATCCTTCAGCCAATGATTGTCTCAGGCATGCTGGAACATGAAA CGATTCAGGGCGTGTCTGGGGTGAAGCCCACAGGGTTGAGAAAGCGAACCTCCAGTATCGCCGATGAGGG CACCTACACACTGGACTCCATCCTCCGGCAGCTCAACTCCTTCCACTCGGTCATGTGTCAGCATGGCATG GACCCTGAACTGATCAAGCAGGTGGTCAAGCAGATGTTCTACATCATAGGGGCCATCACCCTGAACAACC TTCTCCTGCGGAAGGACATGTGCTCCTGGAGTAAAGGCATGCAGATCAGGTACAATGTCAGTCAACTGGA AGAATGGCTGCGTGACAAGAATCTGATGAATAGTGGGGCTAAAGAAACCCTGGAACCTCTCATTCAGGCT GCTCAACTTTTGCAAGTGAAAAAGAAAACAGATGATGATGCAGAAGCCATTTGTTCTATGTGCAATGCTT TAACTACTGCCCAGATTGTGAAAGTGTTGAATTTGTATACTCCAGTTAATGAGTTTGAAGAAAGAGTCTC TGTGTCGTTCATTCGTACTATACAGATGCGTTTACGAGACAGGAAAGACTCTCCCCAGCTGCTCATGGAT GCTAAACACATCTTTCCTGTCACCTTTCCTTTCAACCCATCTTCCCTCGCACTAGAAACCATCCAGATTC CAGCCAGCCTCGGCCTGGGCTTTATTTCACGGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218817 representing NM_000259
Red=Cloning site Green=Tags(s) MAASELYTKFARVWIPDPEEVWKSAELLKDYKPGDKVLLLHLEEGKDLEYHLDPKTKELPHLRNPDILVG ENDLTALSYLHEPAVLHNLRVRFIDSKLIYTYCGIVLVAINPYEQLPIYGEDIINAYSGQNMGDMDPHIF AVAEEAYKQMARDERNQSIIVSGESGAGKTVSAKYAMRYFATVSGSASEANVEEKVLTSNPIMESIGNAK TTRNDNSSRFGKYIEIGFDKRYRIIGANMRTYLLEKSRVVFQAEEERNYHIFYQLCASAKLPEFKMLRLG NADNFNYTKQGGSPVIEGVDDAKEMAHTRQACTLLGISESHQMGIFRILAGILHLGNVGFTSRDADSCTI PPKHEPLCIFCDLMGVDYEEMCHWLCHRKLATATETYIKPISKLQATNARDALAKHIYAKLFNWIVDNVN QALHSAVKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNMHVFKLEQEEYMKEQIPWTLIDFY DNQPCINLIESKLGILDLLDEECKMPKGTDDTWAQKLYNTHLNKCALFEKPRLSNKAFIIQHFADKVEYQ CEGFLEKNKDTVFEEQIKVLKSSKFKMLPELFQDDEKAISPTSATSSGRTPLTRTPAKPTKGRPGQMAKE HKKTVGHQFRNSLHLLMETLNATTPHYVRCIKPNDFKLPFTFDEKRAVQQLRACGVLETIRISAAGFPSR WTYQEFFSRYRVLMKQKDVLSDRKQTCKNVLEKLILDKDKYQFGKTKIFFRAGQVAYLEKLRADKLRAAC IRIQKTIRGWLLRKKYLRMRKAAITMQRYVRGYQARCYAKFLRRTKAATIIQKYWRMYVVRRRYKIRRAA TIVLQSYLRGFLARNRYRKILRGHKAVIIQKRVRGWLARTHYKRSMHAIIYLQCCFRRMMAKRELKKLKI EARSVERYKKLRIGMENKIMQLQRKVDEQNKDYKCLVEKLTNLEGIYNSETEKLRSDLERLQLSEEEAKV ATGRVLSLQEEIAKLRKDLEQTRSEKKCIEEHADRYKQETEQLVSNLKEENTLLKQEKEALNHRIVQQAK EMTETMEKKLVEETKQLELDLNDERLRYQNLLNEFSRLEERYDDLKEEMTLMVHVPKPGHKRTDSTHSSN ESEYIFSSEIAEMEDIPSRTEEPSEKKVPLDMSLFLKLQKRVTELEQEKQVMQDELDRKEEQVLRSKAKE EERPQIRGAELEYESLKRQELESENKKLKNELNELRKALSEKSAPEVTAPGAPAYRVLMEQLTSVSEELD VRKEEVLILRSQLVSQKEAIQPKDDKNTMTDSTILLEDVQKMKDKGEIAQAYIGLKETNRSSALDYHELN EDGELWLVYEGLKQANRLLESQLQSQKRSHENEAEALRGEIQSLKEENNRQQQLLAQNLQLPPEARIEAS LQHEITRLTNENLDLMEQLEKQDKTVRKLKKQLKVFAKKIGELEVGQMENISPGQIIDEPIRPVNIPRKE KDFQGMLEYKKEDEQKLVKNLILELKPRGVAVNLIPGLPAYILFMCVRHADYLNDDQKVRSLLTSTINSI KKVLKKRGDDFETVSFWLSNTCRFLHCLKQYSGEEGFMKHNTSRQNEHCLTNFDLAEYRQVLSDLAIQIY QQLVRVLENILQPMIVSGMLEHETIQGVSGVKPTGLRKRTSSIADEGTYTLDSILRQLNSFHSVMCQHGM DPELIKQVVKQMFYIIGAITLNNLLLRKDMCSWSKGMQIRYNVSQLEEWLRDKNLMNSGAKETLEPLIQA AQLLQVKKKTDDDAEAICSMCNALTTAQIVKVLNLYTPVNEFEERVSVSFIRTIQMRLRDRKDSPQLLMD AKHIFPVTFPFNPSSLALETIQIPASLGLGFISRV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000259 |
| ORF Size | 5565 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000259.1, NP_000250.1 |
| RefSeq Size | 6401 bp |
| RefSeq ORF | 5568 bp |
| Locus ID | 4644 |
| UniProt ID | Q9Y4I1 |
| Gene Summary | This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008] |
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