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PARP2 (NM_005484) Human Tagged ORF Clone
CAT＃: RG223373
- TrueORF®
PARP2 (tGFP-tagged) - Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1
ORF Plasmid: DDK
"NM_005484" in other vectors (5)
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CNY 5,610.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADPRT2; ADPRTL2; ADPRTL3; ARTD2; pADPRT-2; PARP-2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223373 representing NM_005484
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCGGCGGCGACGGAGCACCGGCGGCGGCAGGGCGAGAGCATTAAATGAAAGCAAAAGAGTTA ATAATGGCAACACGGCTCCAGAAGACTCTTCCCCTGCCAAGAAAACTCGTAGATGCCAGAGACAGGAGTC GAAAAAGATGCCTGTGGCTGGAGGAAAAGCTAATAAGGACAGGACAGAAGACAAGCAAGATGGTATGCCA GGAAGGTCATGGGCCAGCAAAAGGGTCTCTGAATCTGTGAAGGCCTTGCTGTTAAAGGGCAAAGCTCCTG TGGACCCAGAGTGTACAGCCAAGGTGGGGAAGGCTCATGTGTATTGTGAAGGAAATGATGTCTATGATGT CATGCTAAATCAGACCAATCTCCAGTTCAACAACAACAAGTACTATCTGATTCAGCTATTAGAAGATGAT GCCCAGAGGAACTTCAGTGTTTGGATGAGATGGGGCCGAGTTGGGAAAATGGGACAGCACAGCCTGGTGG CTTGTTCAGGCAATCTCAACAAGGCCAAGGAAATCTTTCAGAAGAAATTCCTTGACAAAACGAAAAACAA TTGGGAAGATCGAGAAAAGTTTGAGAAGGTGCCTGGAAAATATGATATGCTACAGATGGACTATGCCACC AATACTCAGGATGAAGAGGAAACAAAGAAAGAGGAATCTCTTAAATCTCCCTTGAAGCCAGAGTCACAGC TAGATCTTCGGGTACAGGAGTTAATAAAGTTGATCTGTAATGTTCAGGCCATGGAAGAAATGATGATGGA AATGAAGTATAATACCAAGAAAGCCCCACTTGGGAAGCTGACAGTGGCACAAATCAAGGCAGGTTACCAG TCTCTTAAGAAGATTGAGGATTGTATTCGGGCTGGCCAGCATGGACGAGCTCTCATGGAAGCATGCAATG AATTCTACACCAGGATTCCGCATGACTTTGGACTCCGTACTCCTCCACTAATCCGGACACAGAAGGAACT GTCAGAAAAAATACAATTACTAGAGGCTTTGGGAGACATTGAAATTGCTATTAAGCTGGTGAAAACAGAG CTACAAAGCCCAGAACACCCATTGGACCAACACTATAGAAACCTACATTGTGCCTTGCGCCCCCTTGACC ATGAAAGTTATGAGTTCAAAGTGATTTCCCAGTACCTACAATCTACCCATGCTCCCACACACAGCGACTA TACCATGACCTTGCTGGATTTGTTTGAAGTGGAGAAGGATGGTGAGAAAGAAGCCTTCAGAGAGGACCTT CATAACAGGATGCTTCTATGGCATGGTTCCAGGATGAGTAACTGGGTGGGAATCTTGAGCCATGGGCTTC GAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGTTTGGGAAAGGAATCTACTTTGCTGACATGTC TTCCAAGAGTGCCAATTACTGCTTTGCCTCTCGCCTAAAGAATACAGGACTGCTGCTCTTATCAGAGGTA GCTCTAGGTCAGTGTAATGAACTACTAGAGGCCAATCCTAAGGCCGAAGGATTGCTTCAAGGTAAACATA GCACCAAGGGGCTGGGCAAGATGGCTCCCAGTTCTGCCCACTTCGTCACCCTGAATGGGAGTACAGTGCC ATTAGGACCAGCAAGTGACACAGGAATTCTGAATCCAGATGGTTATACCCTCAACTACAATGAATATATT GTATATAACCCCAACCAGGTCCGTATGCGGTACCTTTTAAAGGTTCAGTTTAATTTCCTTCAGCTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223373 representing NM_005484
Red=Cloning site Green=Tags(s) MAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMPVAGGKANKDRTEDKQDGMP GRSWASKRVSESVKALLLKGKAPVDPECTAKVGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDD AQRNFSVWMRWGRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKYDMLQMDYAT NTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAMEEMMMEMKYNTKKAPLGKLTVAQIKAGYQ SLKKIEDCIRAGQHGRALMEACNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTE LQSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTLLDLFEVEKDGEKEAFREDL HNRMLLWHGSRMSNWVGILSHGLRIAPPEAPITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEV ALGQCNELLEANPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDGYTLNYNEYI VYNPNQVRMRYLLKVQFNFLQLW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005484 |
| ORF Size | 1749 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005484.4 |
| RefSeq Size | 1904 bp |
| RefSeq ORF | 1752 bp |
| Locus ID | 10038 |
| UniProt ID | Q9UGN5 |
| Domains | PARP, PARP_reg |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair |
| Gene Summary | This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223373 | PARP2 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1 |
CNY 6,512.00 |
|
| RC223373L3 | Lenti-ORF clone of PARP2 (Myc-DDK-tagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1 |
CNY 7,030.00 |
|
| RC223373L4 | Lenti-ORF clone of PARP2 (mGFP-tagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1 |
CNY 7,030.00 |
|
| SC308977 | PARP2 (untagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1 |
CNY 5,976.00 |
|
| SC318002 | PARP2 (untagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1 |
CNY 9,500.00 |
