United States
Germany
Japan
United Kingdom
China
ITPKB (NM_002221) Human Tagged ORF Clone
CAT＃: RG223964
- TrueORF®
ITPKB (tGFP-tagged) - Human inositol-trisphosphate 3-kinase B (ITPKB)
ORF Plasmid: DDK
"NM_002221" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IP3-3KB; IP3K; IP3K-B; IP3KB; PIG37 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG223964 representing NM_002221
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGTACTGCTATGCGCTCAATAGCCTGGTGATCATGAATAGCGCCAACGAGATGAAGAGCGGCG GCGGCCCGGGGCCCAGTGGCAGCGAGACGCCCCCGCCCCCGAGGAGGGCAGTGCTGAGCCCCGGCAGCGT TTTCAGCCCCGGGAGAGGCGCCTCTTTCCTCTTCCCCCCAGCCGAGTCGCTGTCCCCCGAGGAGCCCCGG AGCCCCGGGGGCTGGCGGAGCGGCCGGCGCAGGCTGAATAGTAGCAGCGGCAGTGGCAGCGGCAGCAGCG GCAGTAGCGTGAGCAGCCCAAGTTGGGCTGGTCGCCTGCGAGGGGACCGGCAGCAGGTGGTGGCAGCCGG TACCCTCTCCCCGCCAGGGCCGGAGGAGGCCAAGAGGAAGCTGCGGATCTTGCAGCGCGAGTTGCAGAAC GTGCAGGTGAACCAGAAAGTGGGCATGTTTGAGGCGCACATCCAGGCACAGAGCTCCGCCATTCAAGCGC CCCGCAGCCCGCGTTTGGGCAGGGCTCGCTCGCCCTCCCCGTGCCCCTTCCGCAGCAGCAGTCAGCCCCC TGGAAGGGTCCTGGTTCAGGGCGCCCGGAGCGAGGAACGGAGGACAAAGTCCTGGGGGGAGCAATGTCCA GAGACTTCAGGAACCGACTCCGGGAGGAAAGGAGGGCCCAGCCTATGCTCCTCGCAGGTGAAGAAAGGAA TGCCACCTCTTCCCGGCCGGGCTGCCCCTACAGGATCAGAGGCTCAGGGTCCATCCGCTTTTGTAAGGAT GGAGAAGGGTATCCCTGCCAGTCCCCGCTGTGGCTCACCCACAGCTATGGAAATTGACAAAAGGGGCTCT CCTACCCCGGGAACTCGGAGCTGCCTAGCTCCCTCATTGGGGCTGTTCGGAGCTAGCTTAACGATGGCCA CGGAAGTGGCAGCGAGAGTTACATCCACTGGGCCACACCGTCCACAGGATCTTGCCCTCACTGAGCCGTC TGGGAGAGCCCGTGAGCTTGAGGACCTGCAGCCCCCAGAGGCCCTGGTGGAGAGGCAGGGGCAGTTTCTG GGCAGTGAGACAAGCCCAGCCCCAGAAAGGGGCGGGCCCCGCGATGGAGAACCCCCTGGGAAGATGGGGA AAGGATATCTGCCCTGTGGCATGCCGGGCTCTGGGGAGCCTGAAGTGGGCAAAAGGCCAGAGGAGACGAC TGTGAGCGTGCAAAGCGCAGAGTCCTCTGATTCCCTGAGCTGGTCCAGGCTGCCCAGGGCCCTGGCCTCC GTAGGCCCTGAGGAGGCCCGAAGTGGGGCCCCCGTGGGCGGGGGGCGTTGGCAGCTCTCCGACAGAGTGG AGGGAGGGTCCCCAACGCTGGGCTTGCTTGGGGGCAGCCCCTCAGCACAGCCGGGGACCGGGAATGTGGA GGCGGGAATTCCTTCTGGCAGAATGCTGGAGCCTTTGCCCTGTTGGGACGCTGCGAAAGATCTGAAAGAA CCTCAGTGCCCTCCTGGGGACAGGGTGGGTGTGCAGCCTGGGAACTCCAGGGTTTGGCAGGGCACCATGG AGAAAGCCGGTTTGGCTTGGACGCGTGGCACAGGGGTGCAATCAGAGGGGACTTGGGAAAGCCAGCGGCA GGACAGTGATGCCCTCCCAAGTCCGGAGCTGCTACCCCAAGATCAGGACAAGCCTTTCCTGAGGAAGGCC TGCAGCCCCAGCAACATACCTGCTGTCATCATTACAGACATGGGCACCCAGGAGGATGGGGCCTTGGAGG AGACGCAGGGAAGCCCTCGGGGCAACCTGCCCCTGAGGAAACTGTCCTCTTCCTCGGCCTCCTCCACGGG CTTCTCCTCATCCTACGAAGACTCAGAGGAGGACATCTCCAGTGACCCTGAGCGCACCCTGGACCCCAAC TCAGCCTTCCTGCATACCCTGGACCAGCAGAAACCTAGAGTGAGCAAATCATGGAGGAAGATAAAAAACA TGGTGCACTGGTCTCCCTTCGTCATGTCCTTCAAGAAGAAGTACCCCTGGATCCAGCTGGCAGGACACGC AGGGAGTTTCAAGGCAGCTGCCAATGGCAGGATCCTGAAGAAGCACTGTGAGTCAGAGCAGCGCTGCCTG GACCGGCTGATGGTGGATGTGCTGAGGCCCTTCGTACCTGCCTACCATGGGGATGTGGTGAAGGACGGGG AGCGCTACAACCAGATGGACGACCTGCTGGCCGACTTCGACTCGCCCTGTGTGATGGACTGCAAGATGGG AATCAGGACCTACCTGGAGGAGGAGCTCACGAAGGCCCGGAAGAAGCCCAGCCTGCGGAAGGACATGTAC CAGAAGATGATCGAGGTGGACCCCGAGGCCCCCACCGAGGAGGAAAAAGCACAGCGGGCTGTGACCAAGC CACGGTACATGCAGTGGCGGGAGACCATCAGCTCCACGGCCACCCTGGGGTTCAGGATCGAGGGAATCAA GAAAGAAGACGGCACCGTGAACCGGGACTTCAAGAAGACCAAAACGAGGGAGCAGGTCACCGAGGCCTTC AGAGAGTTCACTAAAGGAAACCATAACATCCTGATCGCCTATCGGGACCGGCTGAAGGCCATTCGAACCA CTCTAGAAGTTTCTCCCTTCTTCAAGTGCCACGAGGTCATTGGCAGCTCCCTCCTCTTCATCCACGACAA GAAGGAACAGGCCAAAGTGTGGATGATCGACTTTGGGAAAACCACGCCCCTGCCTGAGGGCCAGACCCTG CAGCATGACGTCCCCTGGCAGGAGGGGAACCGGGAGGATGGCTACCTCTCGGGGCTCAATAACCTCGTCG ACATCCTGACCGAGATGTCCCAGGATGCCCCACTCGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG223964 representing NM_002221
Red=Cloning site Green=Tags(s) MAVYCYALNSLVIMNSANEMKSGGGPGPSGSETPPPPRRAVLSPGSVFSPGRGASFLFPPAESLSPEEPR SPGGWRSGRRRLNSSSGSGSGSSGSSVSSPSWAGRLRGDRQQVVAAGTLSPPGPEEAKRKLRILQRELQN VQVNQKVGMFEAHIQAQSSAIQAPRSPRLGRARSPSPCPFRSSSQPPGRVLVQGARSEERRTKSWGEQCP ETSGTDSGRKGGPSLCSSQVKKGMPPLPGRAAPTGSEAQGPSAFVRMEKGIPASPRCGSPTAMEIDKRGS PTPGTRSCLAPSLGLFGASLTMATEVAARVTSTGPHRPQDLALTEPSGRARELEDLQPPEALVERQGQFL GSETSPAPERGGPRDGEPPGKMGKGYLPCGMPGSGEPEVGKRPEETTVSVQSAESSDSLSWSRLPRALAS VGPEEARSGAPVGGGRWQLSDRVEGGSPTLGLLGGSPSAQPGTGNVEAGIPSGRMLEPLPCWDAAKDLKE PQCPPGDRVGVQPGNSRVWQGTMEKAGLAWTRGTGVQSEGTWESQRQDSDALPSPELLPQDQDKPFLRKA CSPSNIPAVIITDMGTQEDGALEETQGSPRGNLPLRKLSSSSASSTGFSSSYEDSEEDISSDPERTLDPN SAFLHTLDQQKPRVSKSWRKIKNMVHWSPFVMSFKKKYPWIQLAGHAGSFKAAANGRILKKHCESEQRCL DRLMVDVLRPFVPAYHGDVVKDGERYNQMDDLLADFDSPCVMDCKMGIRTYLEEELTKARKKPSLRKDMY QKMIEVDPEAPTEEEKAQRAVTKPRYMQWRETISSTATLGFRIEGIKKEDGTVNRDFKKTKTREQVTEAF REFTKGNHNILIAYRDRLKAIRTTLEVSPFFKCHEVIGSSLLFIHDKKEQAKVWMIDFGKTTPLPEGQTL QHDVPWQEGNREDGYLSGLNNLVDILTEMSQDAPLA SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002221 |
| ORF Size | 2838 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002221.2, NP_002212.2 |
| RefSeq Size | 5875 bp |
| RefSeq ORF | 2841 bp |
| Locus ID | 3707 |
| UniProt ID | P27987 |
| Protein Families | Druggable Genome |
| Protein Pathways | Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | The protein encoded by this protein regulates inositol phosphate metabolism by phosphorylation of second messenger inositol 1,4,5-trisphosphate to Ins(1,3,4,5)P4. The activity of this encoded protein is responsible for regulating the levels of a large number of inositol polyphosphates that are important in cellular signaling. Both calcium/calmodulin and protein phosphorylation mechanisms control its activity. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223964 | ITPKB (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase B (ITPKB) |
CNY 6,928.00 |
|
| RC223964L3 | Lenti ORF clone of Human inositol-trisphosphate 3-kinase B (ITPKB), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC223964L4 | Lenti ORF clone of Human inositol-trisphosphate 3-kinase B (ITPKB), mGFP tagged |
CNY 9,030.00 |
|
| SC303151 | ITPKB (untagged)-Human inositol-trisphosphate 3-kinase B (ITPKB) |
CNY 18,240.00 |
