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UBE4B (NM_001105562) Human Tagged ORF Clone
CAT＃: RG226378
- TrueORF®
UBE4B (tGFP-tagged) - Human ubiquitination factor E4B (UBE4B), transcript variant 1
ORF Plasmid: DDK
"NM_001105562" in other vectors (4)
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CNY 14,350.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E4; HDNB1; UBOX3; UFD2; UFD2A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG226378 representing NM_001105562
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCTGAGCGCTGATGAGATTCGACGGAGGCGCCTTGCACGACTTGCTGGTGGACAGACCTCTC AGCCAACCACCCCACTCACCTCTCCCCAGAGGGAGAACCCTCCGGGGCCTCCCATAGCGGCATCAGCCCC AGGACCCTCTCAGAGTCTTGGTCTCAATGTCCACAACATGACCCCAGCTACCTCCCCAATAGGTGCATCA GGAGTAGCCCATCGAAGCCAGAGCAGTGAAGGAGTCAGTTCTCTCAGCAGCTCGCCCTCTAATAGCCTTG AAACGCAATCTCAGTCTCTCTCACGTTCCCAGAGCATGGATATCGATGGTGTCTCATGTGAGAAAAGCAT GTCCCAGGTGGATGTGGATTCAGGAATTGAAAACATGGAGGTTGATGAAAATGATCGAAGAGAAAAGCGG AGCCTCAGTGATAAGGAGCCTTCCTCGGGCCCTGAAGTGTCTGAAGAGCAGGCCTTACAGCTGGTCTGTA AGATCTTCCGTGTCTCTTGGAAGGACCGGGACAGAGATGTCATCTTTCTTTCTTCTCTTTCTGCACAGTT TAAGCAGAACCCAAAAGAAGTATTCTCCGATTTTAAGGACTTGATTGGCCAGATTTTAATGGAAGTGCTA ATGATGTCCACTCAGACCAGAGATGAAAACCCATTTGCCAGTCTGACAGCCACATCACAGCCAATTGCTG CAGCAGCACGGTCACCAGACAGAAATCTCTTGCTAAACACTGGCTCCAATCCAGGAACAAGCCCCATGTT CTGCAGCGTGGCTTCCTTTGGTGCCAGCTCTTTGTCTAGCCTCTATGAAAGTAGTCCGGCTCCCACTCCC AGTTTCTGGAGCTCTGTTCCCGTGATGGGCCCGTCTCTTGCCTCACCTTCCCGTGCAGCCAGCCAGTTGG CTGTGCCTTCCACTCCCCTCAGTCCTCACAGTGCAGCCTCTGGAACTGCTGCGGGAAGCCAGCCTTCATC CCCGCGGTATCGCCCCTACACTGTCACTCACCCATGGGCGTCCTCAGGCGTCTCCATTCTGTCGAGCTCC CCAAGTCCCCCTGCCCTCGCCAGTAGCCCCCAAGCAGTGCCCGCCAGCAGTTCCAGACAGAGGCCCAGCA GCACGGGTCCACCCCTACCACCCGCCTCACCCAGTGCCACGAGCAGACGCCCCTCCTCCCTGAGGATCTC TCCTAGTTTGGGAGCCTCTGGTGGAGCAAGTAATTGGGATTCCTACAGTGACCATTTCACCATTGAAACC TGCAAAGAGACAGATATGCTGAACTACCTCATCGAGTGTTTCGACCGAGTTGGAATAGAGGAAAAAAAAG CACCAAAGATGTGCAGCCAGCCAGCAGTCAGCCAGCTTCTGAGCAACATCCGCTCACAGTGCATATCCCA TACTGCTTTAGTACTACAAGGCTCCCTAACACAGCCCAGGTCCTTGCAGCAGCCGTCCTTCCTAGTGCCG TATATGCTGTGTAGGAATCTCCCATATGGCTTCATTCAGGAACTGGTGAGAACCACTCACCAGGATGAAG AAGTGTTCAAGCAGATATTTATCCCCATTTTACAAGGCCTGGCTCTTGCTGCCAAAGAGTGCTCCCTCGA CAGTGACTACTTTAAATACCCCCTCATGGCACTAGGTGAGCTCTGTGAAACCAAGTTTGGGAAGACACAC CCTGTGTGCAATTTGGTTGCTTCTTTGCGGTTGTGGTTGCCGAAATCCTTAAGTCCTGGCTGTGGGCGGG AGCTGCAGAGACTCTCTTACTTAGGGGCTTTCTTTAGCTTCTCAGTCTTTGCAGAAGATGATGTTAAAGT GGTTGAAAAATACTTCTCAGGGCCTGCCATTACCCTGGAAAACACTCGTGTGGTTAGCCAATCATTGCAG CATTACTTAGAGCTCGGAAGGCAAGAGCTTTTTAAGATTCTGCATAGTATTTTGTTAAATGGCGAAACCC GTGAGGCTGCTCTCAGTTACATGGCGGCTGTCGTCAATGCCAATATGAAGAAAGCACAGATGCAGACAGA TGATAGATTGGTGTCTACAGATGGATTTATGCTGAATTTCCTTTGGGTACTGCAGCAGCTAAGTACAAAA ATCAAGTTAGAAACAGTTGATCCCACGTATATTTTTCACCCAAGATGTCGGATTACTCTTCCCAATGATG AGACGCGTGTGAATGCAACGATGGAAGATGTGAATGACTGGCTGACTGAACTCTATGGCGATCAGCCTCC ATTTTCTGAGCCGAAATTCCCTACGGAGTGCTTCTTTCTCACCCTGCATGCTCACCACCTCTCTATTCTG CCTAGTTGCCGTCGCTATATCCGCAGACTCCGGGCTATCCGGGAGCTCAATAGAACTGTAGAAGATTTGA AAAATAATGAAAGCCAATGGAAAGATTCCCCACTGGCAACTAGACACCGCGAAATGCTGAAGCGCTGTAA AACTCAGCTTAAGAAACTGGTACGGTGCAAGGCCTGTGCTGATGCTGGCCTACTTGACGAGAGCTTCCTG AGAAGATGTCTGAATTTTTATGGCCTTCTCATTCAGCTGCTGCTCCGCATCCTGGACCCCGCATATCCCG ATATAACACTGCCTTTAAATTCAGATGTCCCCAAGGTATTTGCAGCGTTGCCTGAGTTTTATGTAGAAGA TGTTGCAGAATTTTTATTTTTTATTGTACAATACTCTCCCCAGGCGCTTTATGAGCCCTGTACTCAGGAT ATTGTGATGTTCCTTGTTGTGATGTTGTGCAACCAGAACTACATCCGAAACCCATATTTGGTGGCCAAAC TGGTAGAAGTCATGTTTATGACCAACCCTGCTGTTCAGCCACGAACCCAGAAGTTTTTTGAAATGATTGA GAACCATCCTCTCTCCACCAAGTTGTTGGTACCTTCCCTGATGAAGTTTTATACAGATGTTGAGCATACC GGAGCCACCAGTGAGTTTTATGACAAGTTCACAATTCGCTATCATATTAGCACCATTTTTAAAAGCCTTT GGCAAAACATAGCTCACCATGGCACCTTTATGGAGGAGTTCAACTCCGGGAAGCAGTTTGTTCGCTATAT AAACATGTTGATAAACGACACGACGTTTTTGCTCGATGAAAGTCTGGAGTCTCTGAAGCGAATCCATGAA GTGCAGGAAGAGATGAAGAACAAAGAACAGTGGGACCAGTTGCCCCGGGATCAGCAGCAGGCTCGTCAGT CTCAGCTTGCTCAGGATGAGCGTGTGTCCCGCTCTTACCTCGCCCTGGCCACCGAAACCGTGGACATGTT CCACATCCTCACGAAGCAGGTCCAGAAGCCCTTCCTCAGACCGGAGCTTGGACCCCGATTGGCTGCAATG CTGAACTTTAATCTTCAGCAACTTTGTGGCCCCAAGTGCCGTGACCTGAAAGTTGAAAACCCTGAGAAAT ACGGCTTTGAACCAAAGAAGCTGTTGGACCAACTGACGGATATTTACTTACAGCTGGACTGTGCTCGGTT CGCGAAAGCCATTGCTGACGACCAGAGATCCTACAGTAAGGAATTGTTTGAAGAAGTTATTTCAAAGATG CGGAAGGCAGGGATCAAATCCACAATAGCAATAGAAAAATTTAAGCTGCTCGCCGAGAAAGTGGAGGAGA TAGTGGCCAAGAACGCACGCGCAGAAATCGACTACAGCGACGCTCCTGATGAGTTCAGAGACCCTCTGAT GGACACCCTCATGACAGACCCCGTGCGGCTGCCCTCTGGCACCATCATGGACCGCTCCATCATCCTGCGG CACCTGCTCAACTCCCCCACGGACCCCTTCAACCGGCAGACGCTGACAGAGAGCATGCTGGAACCAGTGC CAGAACTGAAAGAGCAGATTCAGGCGTGGATGAGAGAGAAACAGAACAGCGATCAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226378 representing NM_001105562
Red=Cloning site Green=Tags(s) MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGAS GVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKR SLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVL MMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTP SFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSS PSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIET CKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVP YMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTH PVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQ HYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTK IKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSIL PSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFL RRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQD IVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHT GATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHE VQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAM LNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKM RKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILR HLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001105562 |
| ORF Size | 3906 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001105562.3 |
| RefSeq Size | 5943 bp |
| RefSeq ORF | 3909 bp |
| Locus ID | 10277 |
| UniProt ID | O95155 |
| Protein Pathways | Ubiquitin mediated proteolysis |
| Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226378 | UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 13,600.00 |
|
| RC226378L3 | Lenti-ORF clone of UBE4B (Myc-DDK-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 14,920.00 |
|
| RC226378L4 | Lenti-ORF clone of UBE4B (mGFP-tagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 14,920.00 |
|
| SC317163 | UBE4B (untagged)-Human ubiquitination factor E4B (UBE4B), transcript variant 1 |
CNY 25,180.00 |
