Apolipoprotein L 1 (APOL1) (NM_001136540) Human Tagged ORF Clone
CAT#: RG226566
- TrueORF®
APOL1 (tGFP-tagged) - Human apolipoprotein L, 1 (APOL1), transcript variant 3
ORF Plasmid: DDK
"NM_001136540" in other vectors (4)
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CNY 4,370.00
Cited in 2 publications. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | APO-L; APOL; APOL-I; FSGS4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG226566 representing NM_001136540
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTCCTTGGTGTGG GAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGTGGGACAGATACTGGAGATCC TCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATGGACCCAGAGAGCAGTATCTTTATTGAGGAT GCCATTAAGTATTTCAAGGAAAAAGTGAGCACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCT GGAACGGATTCGTGGCTGCTGCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAA CCTTGCAAGACAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTT CTGAAAGAGTTTCCTCGGTTGAAAAGTAAGCTTGAGGATAACATAAGAAGGCTCCGTGCCCTTGCAGATG GGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGCTCTCTCAGCATTTCCTCTGG CATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACAGAGGGAGGCAGCCTTGTACTCTTGGAACCT GGGATGGAGTTGGGAATCACAGCAGCTTTGACCGGGATTACCAGCAGTACCATAGACTACGGAAAGAAGT GGTGGACACAAGCCCAAGCCCACGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAAGGAGTT TTTGGGTGAGAACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGG AAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCAGCCTCACGCC CCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGAGTTAATGAACCCAGCATCCT GGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCTGTAAGCTTCTTTCTTGTGCTGGATGTAGTC TACCTCGTGTACGAATCAAAGCACTTACATGAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGG TGGCTCAGGAGCTGGAGGAGAAGCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGA ACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG226566 representing NM_001136540
Red=Cloning site Green=Tags(s) MEGAALLRVSVLCIWMSALFLGVGVRAEEAGARVQQNVPSGTDTGDPQSKPLGDWAAGTMDPESSIFIED AIKYFKEKVSTQNLLLLLTDNEAWNGFVAAAELPRNEADELRKALDNLARQMIMKDKNWHDKGQQYRNWF LKEFPRLKSKLEDNIRRLRALADGVQKVHKGTTIANVVSGSLSISSGILTLVGMGLAPFTEGGSLVLLEP GMELGITAALTGITSSTIDYGKKWWTQAQAHDLVIKSLDKLKEVKEFLGENISNFLSLAGNTYQLTRGIG KDIRALRRARANLQSVPHASASRPRVTEPISAESGEQVERVNEPSILEMSRGVKLTDVAPVSFFLVLDVV YLVYESKHLHEGAKSETAEELKKVAQELEEKLNILNNNYKILQADQEL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001136540 |
ORF Size | 1194 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001136540.1, NP_001130012.1 |
RefSeq Size | 2924 bp |
RefSeq ORF | 1197 bp |
Locus ID | 8542 |
UniProt ID | O14791 |
Protein Families | Secreted Protein, Transmembrane |
Gene Summary | This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Blocking the 5′ splice site of exon 4 by a morpholino oligomer triggers APOL1 protein isoform switch
,null,
Scientific Reports
,PubMed ID 29880816
[APOL1]
|
The Innate Immune Factor Apolipoprotein L1 Restricts HIV-1 Infection
,null,
Journal of Virology
,PubMed ID 24173214
[APOL1]
|
Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226566 | APOL1 (Myc-DDK-tagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 3 |
CNY 3,656.00 |
|
RC226566L3 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC226566L4 | Lenti ORF clone of Human apolipoprotein L, 1 (APOL1), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
SC325047 | APOL1 (untagged)-Human apolipoprotein L, 1 (APOL1), transcript variant 3 |
CNY 7,220.00 |
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