OBSL1 (NM_001173408) Human Tagged ORF Clone
CAT＃: RG230623
- TrueORF®
OBSL1 (tGFP-tagged) - Human obscurin-like 1 (OBSL1), transcript variant 3
ORF Plasmid: DDK
"NM_001173408" in other vectors (2)
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CNY 11310.00
货期*
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG230623 representing NM_001173408
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCGAGCTCGGGGGATCAGGGGAGCCCCCCGTGCTTCCTGCGCTTCCCGCGGCCTGTGCGGGTGG TAAGTGGCGCCGAGGCCGAGCTCAAGTGCGTGGTCCTGGGGGAGCCGCCGCCTGTAGTGGTGTGGGAGAA GGGCGGGCAGCAGCTGGCGGCCTCGGAACGCCTGAGCTTCCCGGCGGACGGCGCGGAGCACGGCCTGCTG CTGACCGCCGCACTGCCCACCGACGCGGGGGTCTACGTGTGCCGCGCCCGCAACGCGGCCGGCGAGGCCT ACGCGGCGGCCGCCGTCACCGTGCTGGAGCCGCCGGCCTCCGACCCCGAGCTGCAGCCCGCCGAGCGCCC GCTGCCATCGCCGGGGTCCGGGGAGGGCGCCCCGGTCTTCCTCACGGGGCCTCGATCCCAGTGGGTGCTG CGGGGGGCGGAGGTGGTGCTGACGTGCCGGGCGGGGGGCCTCCCCGAGCCCACACTGTACTGGGAGAAGG ACGGGATGGCCCTGGACGAAGTGTGGGACAGCAGCCACTTCGCGCTCCAGCCGGGCCGCGCCGAGGACGG CCCCGGCGCGAGCCTGGCACTGCGCATCCTGGCGGCTCGGCTGCCGGATTCCGGCGTCTACGTGTGCCAC GCCCGCAACGCGCACGGCCACGCGCAGGCGGGGGCGCTGCTCCAGGTGCACCAGCCCCCCGAGAGCCCGC CCGCGGACCCCGACGAGGCCCCCGCGCCGGTGGTGGAGCCGCTCAAGTGCGCGCCTAAGACCTTCTGGGT GAACGAGGGCAAGCACGCCAAGTTCCGCTGCTACGTGATGGGCAAGCCCGAGCCCGAGATCGAATGGCAC TGGGAGGGCCGCCCGCTGCTCCCGGACCGCCGCCGCCTCATGTACCGCGACCGCGACGGCGGCTTCGTGC TCAAGGTGCTTTACTGCCAGGCCAAGGATCGTGGGCTCTACGTCTGCGCCGCGCGCAACTCGGCGGGCCA GACGCTCAGTGCCGTGCAGCTGCACGTGAAAGAGCCCCGCCTCCGGTTCACACGGCCCCTGCAGGACGTG GAGGGCCGTGAGCACGGGATTGCCGTGCTGGAATGTAAAGTACCCAACTCCCGCATCCCCACGGCCTGGT TCCGTGAGGACCAGCGGCTGCTGCCCTGCCGCAAGTACGAGCAGATCGAAGAGGGCACTGTCCGGCGCCT CATCATCCACAGGCTGAAGGCAGACGATGATGGTATCTACCTGTGCGAGATGCGGGGCCGGGTGCGCACC GTGGCCAACGTCACAGTCAAAGGGCCCATCCTGAAGCGCCTGCCCCGGAAGCTCGACGTCCTGGAAGGAG AGAATGCTGTGCTGCTAGTGGAAACTCTAGAGGCCGGGGTCGAGGGACGCTGGAGCCGTGATGGGGAGGA GCTGCCGGTCATCTGCCAGAGCAGCTCAGGCCACATGCATGCCCTGGTCCTTCCAGGGGTCACCCGAGAG GATGCTGGCGAGGTCACCTTTAGCCTGGGCAACTCCCGTACCACTACGCTTCTCAGAGTAAAATGTGTCA AGCACAGTCCCCCAGGACCCCCCATATTGGCAGAGATGTTCAAGGGCCACAAGAACACGGTCCTGTTGAC CTGGAAGCCTCCCGAGCCAGCTCCCGAGACCCCATTCATCTACCGGCTGGAGCGGCAGGAAGTGGGCTCT GAAGACTGGATTCAGTGCTTCAGCATCGAGAAAGCCGGAGCCGTGGAGGTGCCGGGCGACTGTGTGCCCT CCGAGGGTGACTACCGCTTCCGCATCTGCACAGTCAGCGGACATGGCCGTAGTCCCCACGTGGTGTTCCA CGGTTCTGCTCACCTTGTGCCCACAGCTCGCCTGGTGGCAGGTCTGGAGGATGTGCAGGTATACGACGGG GAAGATGCCGTCTTCTCCCTCGATCTCTCCACCATCATCCAGGGTACCTGGTTCCTTAATGGGGAAGAGC TCAAGAGTAACGAGCCGGAGGGCCAGGTGGAACCTGGGGCCCTGCGGTACCGTATAGAGCAGAAGGGTCT GCAGCACAGACTCATCCTGCATGCCGTCAAGCACCAGGACAGCGGTGCCCTGGTCGGCTTCAGCTGCCCC GGCGTGCAGGACTCAGCTGCCCTCACAATCCAAGAGAGCCCGGTGCACATCCTGAGCCCCCAGGACAGGG TGTCGTTGACCTTCACAACCTCAGAGCGGGTGGTGCTGACTTGTGAGCTCTCAAGGGTGGACTTCCCGGC AACCTGGTACAAGGATGGGCAGAAGGTGGAGGAGAGCGAGTTGCTGGTGGTGAAGATGGATGGGCGCAAA CACCGTCTGATCCTGCCTGAGGCCAAAGTCCAGGACAGTGGCGAGTTTGAGTGCAGGACAGAAGGGGTCT CGGCCTTCTTCGGCGTCACTGTCCAAGATCCTCCCGTGCACATCGTGGACCCCCGAGAACATGTGTTCGT GCATGCCATAACTTCCGAGTGTGTCATGCTGGCCTGTGAGGTGGACCGAGAGGACGCCCCTGTGCGTTGG TACAAGGACGGGCAGGAGGTGGAGGAGAGTGACTTCGTGGTGCTGGAGAATGAGGGGCCCCATCGCCGCC TGGTGCTGCCCGCCACCCAGCCCTCAGACGGGGGCGAGTTTCAGTGCGTCGCTGGAGATGAGTGTGCCTA CTTCACTGTCACCATCACAGACGTCTCCTCGTGGATCGTGTATCCCAGCGGCAAGGTGTATGTGGCAGCC GTGCGCCTGGAGCGTGTGGTGCTGACCTGTGAGCTATGCCGGCCCTGGGCAGAGGTGCGCTGGACCAAGG ATGGAGAGGAGGTGGTGGAGAGCCCCGCGCTGCTCCTGCAGAAGGAAGACACTGTCCGCCGCCTGGTGCT GCCCGCTGTCCAGCTCGAGGACTCCGGCGAGTACTTGTGTGAAATTGACGATGAGTCGGCCTCCTTCACT GTCACCGTCACAGAGTCTTACCAAAGTCAGGACAGTTCAAATAACAATCCGGAGTTATGCGTCCTCTTGA AAAAGCCGAAGACCCGGCGGCTCTGGTCCCGCTTCCCCCCATGGCGACGAACAGCTGGCACTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG230623 representing NM_001173408
Red=Cloning site Green=Tags(s) MKASSGDQGSPPCFLRFPRPVRVVSGAEAELKCVVLGEPPPVVVWEKGGQQLAASERLSFPADGAEHGLL LTAALPTDAGVYVCRARNAAGEAYAAAAVTVLEPPASDPELQPAERPLPSPGSGEGAPVFLTGPRSQWVL RGAEVVLTCRAGGLPEPTLYWEKDGMALDEVWDSSHFALQPGRAEDGPGASLALRILAARLPDSGVYVCH ARNAHGHAQAGALLQVHQPPESPPADPDEAPAPVVEPLKCAPKTFWVNEGKHAKFRCYVMGKPEPEIEWH WEGRPLLPDRRRLMYRDRDGGFVLKVLYCQAKDRGLYVCAARNSAGQTLSAVQLHVKEPRLRFTRPLQDV EGREHGIAVLECKVPNSRIPTAWFREDQRLLPCRKYEQIEEGTVRRLIIHRLKADDDGIYLCEMRGRVRT VANVTVKGPILKRLPRKLDVLEGENAVLLVETLEAGVEGRWSRDGEELPVICQSSSGHMHALVLPGVTRE DAGEVTFSLGNSRTTTLLRVKCVKHSPPGPPILAEMFKGHKNTVLLTWKPPEPAPETPFIYRLERQEVGS EDWIQCFSIEKAGAVEVPGDCVPSEGDYRFRICTVSGHGRSPHVVFHGSAHLVPTARLVAGLEDVQVYDG EDAVFSLDLSTIIQGTWFLNGEELKSNEPEGQVEPGALRYRIEQKGLQHRLILHAVKHQDSGALVGFSCP GVQDSAALTIQESPVHILSPQDRVSLTFTTSERVVLTCELSRVDFPATWYKDGQKVEESELLVVKMDGRK HRLILPEAKVQDSGEFECRTEGVSAFFGVTVQDPPVHIVDPREHVFVHAITSECVMLACEVDREDAPVRW YKDGQEVEESDFVVLENEGPHRRLVLPATQPSDGGEFQCVAGDECAYFTVTITDVSSWIVYPSGKVYVAA VRLERVVLTCELCRPWAEVRWTKDGEEVVESPALLLQKEDTVRRLVLPAVQLEDSGEYLCEIDDESASFT VTVTESYQSQDSSNNNPELCVLLKKPKTRRLWSRFPPWRRTAGTE TRTRPLE - GFP Tag - V |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NM_001173408 |
| ORF Size | 3075 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001173408.2 |
| RefSeq Size | 3578 bp |
| RefSeq ORF | 3078 bp |
| Locus ID | 23363 |
| UniProt ID | O75147 |
| Gene Summary | Cytoskeletal adaptor proteins function in linking the internal cytoskeleton of cells to the cell membrane. This gene encodes a cytoskeletal adaptor protein, which is a member of the Unc-89/obscurin family. The protein contains multiple N- and C-terminal immunoglobulin (Ig)-like domains and a central fibronectin type 3 domain. Mutations in this gene cause 3M syndrome type 2. Alternatively spliced transcript variants encoding different isoforms have been found in this gene. [provided by RefSeq, Mar 2010] |
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