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AP4B1 (NM_001253853) Human Tagged ORF Clone
CAT＃: RG234632
- TrueORF®
AP4B1 (tGFP-tagged) - Homo sapiens adaptor-related protein complex 4, beta 1 subunit (AP4B1), transcript variant 3
ORF Plasmid: DDK
"NM_001253853" in other vectors (2)
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CNY 7,030.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BETA-4; CPSQ5; SPG47 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234632 representing NM_001253853
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCGAGGGCTGGCGTTACGGAGCATGTGTAGCCTCAGGATGCCTGGTGTGCAGGAGTATATACAAC AGCCTATTCTCAATGGTCTGCGGGATAAGGCTTCATATGTCAGGAGAGTGGCAGTCCTTGGATGTGCCAA GATGCATAATCTTCATGGAGACTCTGAAGTAGATGGTGCCCTGGTAAATGAATTATACAGTTTGCTGCGT GACCAGGATCCAATTGTAGTTGTGAACTGCTTGAGGTCTCTAGAGGAAATTCTGAAACAGGAAGGAGGCG TTGTCATCAATAAGCCCATTGCTCACCATCTCTTAAATCGAATGTCAAAACTGGACCAATGGGGCCAGGC TGAAGTATTGAACTTTCTGCTACGCTACCAACCCCGCAGTGAGGAAGAACTATTTGACATTCTCAATCTG TTGGATAGTTTCCTCAAGAGCAGTAGCCCAGGTGTGGTGATGGGAGCTACCAAACTTTTTCTGATCTTGG CAAAAATGTTTCCCCACGTACAAACTGATGTCCTTGTGCGGGTCAAGGGACCTTTGCTAGCTGCCTGTTC TTCAGAGAGCCGTGAGCTCTGTTTTGTTGCTCTTTGTCATGTACGCCAGATCTTGCATAGTTTACCAGGT CACTTTAGCAGCCACTACAAAAAGTTTTTTTGCTCCTACTCGGAGCCCCACTACATCAAACTACAGAAAG TGGAGGTGCTGTGTGAACTGGTGAACGATGAGAATGTGCAGCAGGTGCTAGAGGAGCTTCGAGGGTACTG CACGGATGTGTCTGCGGACTTTGCACAGGCTGCCATCTTTGCCATAGGTGGCATTGCCAGGACTTACACA GATCAATGTGTTCAGATTTTAACAGAGTTGCTGGGTCTTCGACAAGAGCACATTACCACAGTGGTGGTGC AGACTTTCCGAGACCTGGTTTGGTTGTGTCCTCAGTGTACTGAAGCTGTATGTCAGGCCCTGCCCGGCTG TGAAGAGAACATTCAAGATAGTGAGGGGAAGCAAGCACTTATTTGGCTACTTGGTGTCCATGGGGAAAGA ATTCCTAATGCTCCTTATGTGTTAGAGGACTTTGTTGAGAATGTGAAGTCGGAAACATTTCCAGCTGTTA AGATGGAGCTGCTCACTGCTTTGCTGCGCCTTTTCCTCTCCCGACCTGCTGAGTGCCAGGACATGCTAGG ACGTTTGTTGTATTACTGCATAGAGGAAGAAAAAGATATGGCTGTACGGGACCGAGGTCTCTTCTATTAT CGCCTCCTCTTAGTTGGCATTGATGAAGTTAAGCGGATTCTGTGTAGCCCTAAATCTGACCCTACTCTTG GACTTTTGGAGGATCCGGCAGAAAGACCTGTGAATAGCTGGGCCTCAGACTTCAACACACTGGTGCCAGT GTATGGCAAAGCCCACTGGGCAACTATCTCTAAATGCCAGGGGGCAGAGCGTTGTGACCCAGAGCTTCCT AAAACTTCATCCTTTGCCGCATCAGGACCCTTGATTCCTGAAGAGAACAAGGAGAGGGTACAAGAACTCC CTGATTCTGGAGCCCTCATGCTAGTCCCCAATCGCCAGCTTACTGCTGATTATTTTGAGAAAACTTGGCT TAGCCTTAAAGTTGCTCATCAGCAAGTGTTGCCTTGGCGGGGAGAATTCCATCCTGACACCCTCCAGATG GCTCTTCAAGTAGTGAACATCCAGACCATCGCAATGAGTAGGGCTGGGTCTCGGCCATGGAAAGCATACC TCAGTGCTCAGGATGATACTGGCTGTCTGTTCTTAACAGAACTGCTATTGGAGCCTGGAAACTCAGAAAT GCAGATCTCTGTGAAACAAAATGAAGCAAGAACGGAGACGCTGAATAGTTTTATTTCTGTATTAGAAACT GTGATTGGAACAATTGAAGAAATAAAATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234632 representing NM_001253853
Red=Cloning site Green=Tags(s) MVRGLALRSMCSLRMPGVQEYIQQPILNGLRDKASYVRRVAVLGCAKMHNLHGDSEVDGALVNELYSLLR DQDPIVVVNCLRSLEEILKQEGGVVINKPIAHHLLNRMSKLDQWGQAEVLNFLLRYQPRSEEELFDILNL LDSFLKSSSPGVVMGATKLFLILAKMFPHVQTDVLVRVKGPLLAACSSESRELCFVALCHVRQILHSLPG HFSSHYKKFFCSYSEPHYIKLQKVEVLCELVNDENVQQVLEELRGYCTDVSADFAQAAIFAIGGIARTYT DQCVQILTELLGLRQEHITTVVVQTFRDLVWLCPQCTEAVCQALPGCEENIQDSEGKQALIWLLGVHGER IPNAPYVLEDFVENVKSETFPAVKMELLTALLRLFLSRPAECQDMLGRLLYYCIEEEKDMAVRDRGLFYY RLLLVGIDEVKRILCSPKSDPTLGLLEDPAERPVNSWASDFNTLVPVYGKAHWATISKCQGAERCDPELP KTSSFAASGPLIPEENKERVQELPDSGALMLVPNRQLTADYFEKTWLSLKVAHQQVLPWRGEFHPDTLQM ALQVVNIQTIAMSRAGSRPWKAYLSAQDDTGCLFLTELLLEPGNSEMQISVKQNEARTETLNSFISVLET VIGTIEEIKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001253853 |
| ORF Size | 1920 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001253853.3 |
| RefSeq Size | 2663 bp |
| RefSeq ORF | 1923 bp |
| Locus ID | 10717 |
| UniProt ID | Q9Y6B7 |
| Protein Pathways | Lysosome |
| Gene Summary | This gene encodes a subunit of a heterotetrameric adapter-like complex 4 that is involved in targeting proteins from the trans-Golgi network to the endosomal-lysosomal system. Mutations in this gene are associated with cerebral palsy spastic quadriplegic type 5 (CPSQ5) disorder. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC234632 | AP4B1 (Myc-DDK tagged) - Homo sapiens adaptor-related protein complex 4, beta 1 subunit (AP4B1), transcript variant 3 |
CNY 6,370.00 |
|
| SC332244 | AP4B1 (untagged) - Homo sapiens adaptor-related protein complex 4, beta 1 subunit (AP4B1), transcript variant 3 |
CNY 6,270.00 |
