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Bile salt activated lipase (CEL) (NM_001807) Human Tagged ORF Clone
CAT＃: RG234842
- TrueORF®
CEL (tGFP-tagged) - Homo sapiens carboxyl ester lipase (CEL)
ORF Plasmid: DDK
"NM_001807" in other vectors (2)
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CNY 6,370.00
货期*
5周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BAL; BSDL; BSSL; CEase; CELL; FAP; FAPP; LIPA; MODY8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG234842 representing NM_001807
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCACCATGGGGCGCCTGCAACTGGTTGTGTTGGGCCTCACCTGCTGCTGGGCAGTGGCGAGTGCCG CGAAGCTGGGCGCCGTGTACACAGAAGGTGGGTTCGTGGAAGGCGTCAATAAGAAGCTCGGCCTCCTGGG TGACTCTGTGGACATCTTCAAGGGCATCCCCTTCGCAGCTCCCACCAAGGCCCTGGAAAATCCTCAGCCA CATCCTGGCTGGCAAGGGACCCTGAAGGCCAAGAACTTCAAGAAGAGATGCCTGCAGGCCACCATCACCC AGGACAGCACCTACGGGGATGAAGACTGCCTGTACCTCAACATTTGGGTGCCCCAGGGCAGGAAGCAAGT CTCCCGGGACCTGCCCGTTATGATCTGGATCTATGGAGGCGCCTTCCTCATGGGGTCCGGCCATGGGGCC AACTTCCTCAACAACTACCTGTATGACGGCGAGGAGATCGCCACACGCGGAAACGTCATCGTGGTCACCT TCAACTACCGTGTCGGCCCCCTTGGGTTCCTCAGCACTGGGGACGCCAATCTGCCAGGTAACTATGGCCT TCGGGATCAGCACATGGCCATTGCTTGGGTGAAGAGGAATATCGCGGCCTTCGGGGGGGACCCCAACAAC ATCACGCTCTTCGGGGAGTCTGCTGGAGGTGCCAGCGTCTCTCTGCAGACCCTCTCCCCCTACAACAAGG GCCTCATCCGGCGAGCCATCAGCCAGAGCGGCGTGGCCCTGAGTCCCTGGGTCATCCAGAAAAACCCACT CTTCTGGGCCAAAAAGGTGGCTGAGAAGGTGGGTTGCCCTGTGGGTGATGCCGCCAGGATGGCCCAGTGT CTGAAGGTTACTGATCCCCGAGCCCTGACGCTGGCCTATAAGGTGCCGCTGGCAGGCCTGGAGTACCCCA TGCTGCACTATGTGGGCTTCGTCCCTGTCATTGATGGAGACTTCATCCCCGCTGACCCGATCAACCTGTA CGCCAACGCCGCCGACATCGACTATATAGCAGGCACCAACAACATGGACGGCCACATCTTCGCCAGCATC GACATGCCTGCCATCAACAAGGGCAACAAGAAAGTCACGGAGGAGGACTTCTACAAGCTGGTCAGTGAGT TCACAATCACCAAGGGGCTCAGAGGCGCCAAGACGACCTTTGATGTCTACACCGAGTCCTGGGCCCAGGA CCCATCCCAGGAGAATAAGAAGAAGACTGTGGTGGACTTTGAGACCGATGTCCTCTTCCTGGTGCCCACC GAGATTGCCCTAGCCCAGCACAGAGCCAATGCCAAGAGTGCCAAGACCTACGCCTACCTGTTTTCCCATC CCTCTCGGATGCCCGTCTACCCCAAATGGGTGGGGGCCGACCATGCAGATGACATTCAGTACGTTTTCGG GAAGCCCTTCGCCACCCCCACGGGCTACCGGCCCCAAGACAGGACAGTCTCTAAGGCCATGATCGCCTAC TGGACCAACTTTGCCAAAACAGGGGACCCCAACATGGGCGACTCGGCTGTGCCCACACACTGGGAACCCT ACACTACGGAAAACAGCGGCTACCTGGAGATCACCAAGAAGATGGGCAGCAGCTCCATGAAGCGGAGCCT GAGAACCAACTTCCTGCGCTACTGGACCCTCACCTATCTGGCGCTGCCCACAGTGACCGACCAGGAGGCC ACCCCTGTGCCCCCCACAGGGGACTCCGAGGCCACTCCCGTGCCCCCCACGGGTGACTCCGAGACCGCCC CCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGT GCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCG CCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCA CGGGTGACTCCGGGGCCCCCCCCGTGCCGCCCACGGGTGACTCCGGCGCCCCCCCCGTGCCGCCCACGGG TGACGCCGGGCCCCCCCCCGTGCCGCCCACGGGTGACTCCGGCGCCCCCCCCGTGCCGCCCACGGGTGAC TCCGGGGCCCCCCCCGTGACCCCCACGGGTGACTCCGAGACCGCCCCCGTGCCGCCCACGGGTGACTCCG GGGCCCCCCCTGTGCCCCCCACGGGTGACTCTGAGGCTGCCCCTGTGCCCCCCACAGATGACTCCAAGGA AGCTCAGATGCCTGCAGTCATTAGGTTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG234842 representing NM_001807
Red=Cloning site Green=Tags(s) MLTMGRLQLVVLGLTCCWAVASAAKLGAVYTEGGFVEGVNKKLGLLGDSVDIFKGIPFAAPTKALENPQP HPGWQGTLKAKNFKKRCLQATITQDSTYGDEDCLYLNIWVPQGRKQVSRDLPVMIWIYGGAFLMGSGHGA NFLNNYLYDGEEIATRGNVIVVTFNYRVGPLGFLSTGDANLPGNYGLRDQHMAIAWVKRNIAAFGGDPNN ITLFGESAGGASVSLQTLSPYNKGLIRRAISQSGVALSPWVIQKNPLFWAKKVAEKVGCPVGDAARMAQC LKVTDPRALTLAYKVPLAGLEYPMLHYVGFVPVIDGDFIPADPINLYANAADIDYIAGTNNMDGHIFASI DMPAINKGNKKVTEEDFYKLVSEFTITKGLRGAKTTFDVYTESWAQDPSQENKKKTVVDFETDVLFLVPT EIALAQHRANAKSAKTYAYLFSHPSRMPVYPKWVGADHADDIQYVFGKPFATPTGYRPQDRTVSKAMIAY WTNFAKTGDPNMGDSAVPTHWEPYTTENSGYLEITKKMGSSSMKRSLRTNFLRYWTLTYLALPTVTDQEA TPVPPTGDSEATPVPPTGDSETAPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVP PTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDSGAPPVPPTGDAGPPPVPPTGDSGAPPVPPTGD SGAPPVTPTGDSETAPVPPTGDSGAPPVPPTGDSEAAPVPPTDDSKEAQMPAVIRF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001807 |
| ORF Size | 2268 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001807.4, NP_001798.2 |
| RefSeq Size | 2386 bp |
| RefSeq ORF | 2262 bp |
| Locus ID | 1056 |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycerolipid metabolism, Metabolic pathways, Steroid biosynthesis |
| Gene Summary | The protein encoded by this gene is a glycoprotein secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk. The physiological role of this protein is in cholesterol and lipid-soluble vitamin ester hydrolysis and absorption. This encoded protein promotes large chylomicron production in the intestine. Also its presence in plasma suggests its interactions with cholesterol and oxidized lipoproteins to modulate the progression of atherosclerosis. In pancreatic tumoral cells, this encoded protein is thought to be sequestrated within the Golgi compartment and is probably not secreted. This gene contains a variable number of tandem repeat (VNTR) polymorphism in the coding region that may influence the function of the encoded protein. [provided by RefSeq, Jul 2008] |
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