Nrap (NM_001107443) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200097 representing NM_001107443 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATGCAGGCCTGCTCTAGGTGTGGCTATGGGGTTTACCCTGCTGAGAAGGTCAGCTGCATAGACC AGACATGGCACAAAGCATGTTTTCACTGCGAGGTCTGCAAGATGATGCTTTCTGTTAATAACTTCGTGAG TCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACGTTCACTAGTGTCTACCGCACA CCTTTAAATCTGACCTTGAAGAATTCTGTGGCAGCCATGGGTGGGACCGACGGCAAAGAAGACGGCGAAC AGTTTAAATCCGTTCTCCATTGGGACATGAAGTCCAAGGCCGGAGCGGGGGCGGCTAGCCGGCTGATGAA CGAGAGAGATTACTGGCCAGGGTATGGAGAAGGGAACACTTGGTGTCCAGGAGCTCTTCCAGACCCTGAG ATTGTAAGGATGGTTGAAGCTCGACAGTCTCTCCGTGAGGGGTACACAGAAGATGGTGAGCAACCGCAAG GCAAAGGGAGCTTCCCAGCCATGATCACCCCTGCCTACCAACGGGCCAAGGCAGCCAACCAGCTGGCCAG CCAGGTGCAATACAAGAGAGGCCACGATGAACGGGTCTCCACGTTCACTCCGGTGGCAGACACTCCCGAG CTGCTACGGGCCAATGCTGGGGGACAGCTTCAAAATGATGTGAGATACACGGAAGACGGAGGGCAGCAGA GAGGGAAAGGCAGTTTCCCTGCTATGATCACACCAGCGTATCAGATAGCCAAAAGAGCCGCCGAGCTGGC CAGCGATGTGAGGTACCATCAACAATATCACAGAGAAATGAAGGGAGTGGCTAGTCCTGTCGGAGCTGAG GGCACGATGACAAAGGACTCTATAGACCGATGTGGCCAGACTTACTCAGAAGAGTGTGAGGAACATAGGG GAAAGGGCAGCTTCCCGGCCATGATCACCCCAGCTTATCAGAACGCCAAGAAGGCCAACGAACTTGCTAG TGACATAAAGTACAGGCAGGACTTCCATAAGATGAAGGGCGCGGCACATTTTCACTCCCTTGCAGCCCAG GACAACTTGGTTCTCCAGCGGGCTCAGAATGTGAGCAAGCTTGTGAGTGAGAATAAGTATAAAGAAAACT ATCAGACTCACCTGAGAGGCCACTATGAAGGAGTCGGCATGGACAGACGCATGCTCCACGCTCTCAAAGT CGGCAGCCTGGCCAGCAATGTTGCCTACAAGGCTGACTATAAACACGACGTTGTGGATTACAACTACCCA GCCACTGCCACTCCGTTCTACCAAACCACGATGAAGCTGGCTCCCCTGAAGGATGTCAACTACAGGCAGA ACATCGACAGACTGAAGTTCAGCTCGGTGACAAACACGCCACAGATTGTTCAAGCTAAAATCAACGCCCA GCAGCTGAGCCATGTGAATTACCGAGCTGATTACGAGAGAAACAAGCTGAACTACACATTGCCCCAGGAC GCTCCTCAGATGCTGAAGGCGAAGGCCAACGCGCAACTGTTCAGCGAGGTGAAGTACAAAGAAGGCTGGC AGAAGACAAAGGGGAAAGGATTTGAGATGAAGCTGGACGCCATGTCTCTGTTGGCCGCCAAAGCCTCGGG GGAGCTTGCTAGCAGCATTAAATACAAAGAAGAGTATGAGAAAACAAAGGGCAGAGTCCTGGGAACCACC GACTCCAAGCTTCTGCATTCTCTGCAGGTGGCCAAGATGAGCAGCGAGGTGGAATATAAGAAAGGGTTTG AGGAGAGTAAAACCCACTTCCACTTGCCCATGGACATGGTGAACCTCAGGCATGCTAAGAAGGCCCAGGC CCTCGCCAGCGACCTCGACTACAGAAAGAAACTGCATGACTACACAGTGCTGCCTGAAGATCTGAAAACT CAGTGGGCCAAGAAAGCCCACGGGCTCCAGAGTGAGCTGCAGTACAAAGCTGACCTGGCATGGATGAAGG GTGTCGGGTGGCTGACGGAGGGGAGTCTCAACCTGGAGCAGGCGAAGAAGGCTGGACAGCTCGTCAGCGA GAAACACTACCGGCAGAAGGTTGACGAGCTAAAGTTCACCAGTGTGACTGACAGCTCCCAGATGGAGCAC GCCAAGAAGAGCCAGGAGCTGCAGAATGCGGTGGCCTACAAGGCAGGAAACGAGCAGTCCGTCCATCAGT ACACCATCAGCAAAGACGAACCCTTCTTCCTACGAGCCCGGGCCAATGCTGCTCAACTCAGCGAGACCCT GTACAAGAGCAGCTGGGAAAAGCAGAAGGCAAAAGGATTTGAGCTGAGACTTGACTCCTTGACATTCCTG ACAGCCAAAGCCAAGAGAGACCTGGCTAGTGAGGTGAAGTACAAGGAGGACTATGAAAGGTCCAGAGGGA AACTCATCGGGGCGAAGAGCGCACAGGGGGATTCGCAGATGAGCCACTCGCTGCAGATGTCTAAGCTACA GAGTGAACTGGAGTACAAGAAGGGCTTTGAAGATACCAGATCTCAGTACCACGTCTCCCTCGACATGATT CACCTCGCCCATGCTCGCCAGGCGCAGCACTTAGCCACAGATGTAGGCTACAGGACGGCGTCGCACTGTT TCACAGCTCTGCCCACAGACATGAAGGTGGAGTGGGCCAAGAAGGCCTACAGCCTGCAGAGTGATAACCA ATACAGGGCAGACATGAAGTGGATGAAAGGCACAGGCTGGGTGGCCACCGGGTCATTAAATGTGGAGCAG GCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAGTACCGTCAGCATCCAGATGCTTTGAAGTTTACCA GTATTAAAGACACTCCGGAGATGGTCCAGGCCAGGATTAGTTATACCCAAGCAGTGGATAGACTATACAG GGAGCAAGGAGAGAACTTAAAGCACCATTACACGCAGACTGTAGACTTGCCCGAGGTCCTGCTGGCCAAG CTGAACGCCATGAACATCAGCGAGACTCGCTATAAGGAGTCTTGGAGCCGGCTTCGAGACGGCGGGTATA AGCTGAGGCTGGATGCCCTTCCGTTCCAGGCAGCAAAGGCGTCCAGTGAAGTTATAAGTGACTACAAATA CAAAGAAGCATTTGAGAGAATGAAAGGGCAGCTGCTCGGCTCCCGGAGCTTGGAAGATGACCTCAGCCTT GCACATTCAGTACACGCGACCTCACTGCAGAGTGATGTGAATTATAAGAAAGGCTTTGAACACTCAAAGG CTCACTTCCATCTGCCGCTGGATATGGTAACCTTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAA CCAGGACTACAGACACCCGCTCCCCCAACACACATCCTTGGCAGAAGACCTGAGGCTGCGCTGTGCCAAG AAAGCTCACAAGTTACAGAGTGAGAATCTGTACCGATCGGACTTGAACTTCATGCGTGGCGTTCCCTGTG TCATTCCTGGAGCACTGGAGATTGAAGGAAGAAAGAAAGCCTCTGAGCTCATCAGTGAGAGTAAATACCG CCAGCATCCTGGGTCATTCAAGTACACAGCTGTGACAGACACTCCCAACCTTCTGCATGCCAAGTTCAGC AACCAGATCACCAATGAGCGTCTCTATAAAGCAGCTGGTGAGGATGCGCGGCACCAATACACGATGACCC TTGGTCTGCCTGAATTCATCCGAGCCAAAACAAACGCAGCCAACCTGAGCGAGGCAAAATACAAGGAGTC TTGGCATAATCTTCGTGCTCAAGGCTACAAGCTAACGATAGACGCCCTCCCTTTCCAGGCTGCGCGGGCC TCTGGAGACATAGCCAGTGACTTTCTCTACAGGCACGAGTTTGTGAAGGAGCGCGGGCAGCTCATTGGGG TGCGGAGTGTGAGTGACGACCCACGGCTGCGGCACTGCCAACGGATGGGCCAGCTGCAGAGTGAGAATTG GTACAGGAAGGAGGCGGCCAGCAGCCAAGCTCAGTACCACCTGCCCATGGATATGGTGCCCCTGGTCCAT GCCAGGAAGGCCCAGGCCCTGGCTAGTGACCACGACTACCGGACACAGTGCCATGAGTTCACCGCGCTGC CTGAGGATCTGAAGATGGCCTGGGCCAAGAAAGCGCATGCCCTGCAGAGTGAGTTTCGCTATAAAGCGGA CTTGATGGGCATGAAAGGAAGTGGATGGCTGGCTCTGCAATCTCCACAGATAGAGAGTGCAAAGAAGGCT GGAGAGCTCATCAGTGAGACCAAGTACCGTGAAAAACCAGACAGCATCAAGTTCACTACGGTGGTTGATT CTCCAGACCTCGTTCACGCCAGGAAGAGCTACGAGCACTGTAACGAGCGCCTGTACAGGTTGGGAGATGC AGACTCTCTACATAGGTACACCCCAATCGCAGACCATCCCGATTTCACCCGAGCCCGCTTGAACGCAATG CATCTGAGTGACAAAGTCTACAGAAATTCCTGGGAGAAGAACCGGGCTGGCGGCTATGACTTCAGGCTGG ATGCTATCCCGTTCCAGACTGCACGGGCATCCAGGGATATTGCCAGTGATTTCCGGTACAAAGAGGCATT TCTGAGGGACCGGGGCCTACAGATTGGGTATCGCAGCGTCAATGACGACCCAAGGATGAGACATTTCCTC CGAGTCGGCAGGCTCCAAAGTGACAATGAGTACAGAAAGGACTTCGCCAAAGGTCGCTCCCAGTTCCACA GTCGTCCTGACCAACCTGGCTTCCTCCAGGCCAAGAGGAGCCAGCAACTGGCCAGTGATGTGCTCTACAG GCAGCCACTGCCCCAGCACACCAGCGACCCAGAAGAGCTGGGCCTGAAGCACGCCCGGAAGGCTCATCAG CTACAGAGTGATGTCAAGTACAAATCTGACTTGAACCTGACTCGAGGTGTTGGCTGGACCCCTCCTGGCT CCTACAAAGTGGAAATGGCTCGTCGGGCTGCGGAACTGGCCAACAGGAGGGGTCTGGGGATCCAGGGGGC TTCTGTGGAAACAGAGGCAGTGGAGCTTGGAGATCATCGGAGCAGGGGAGTGAACCCTGATGCCTCGGAG ATCCTACATATCAACAGGAAGAAGACTCTGCTGATGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107443
Insert Size	5079 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107443.2, NP_001100913.2
RefSeq Size	5378 bp
RefSeq ORF	5079 bp
Locus ID	307982

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