Cgnl1 (NM_001108164) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1304623
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200642 representing NM_001108164 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTATTTTGGTGAATATCAACACGTGCAGCAGGAATACGGTGTCCACCTGAGACTTGCCAGTG ATGGCACTCCAAAGCCAAGGAATTCCCAGCCCTCAAAGGCAGGCTCCTATGGTGTCAGTATCAGAGTCCA GGGAATTGATGGCCACCCTTACATAGTCCTGAATAACACTGAACGGTGTCTAGCAGGCATACCTTTCCCA GAAAACAGGCCGTTTCCATCTCCGATGATAAATAACTTGTCCCTACATCCAAGCAATGGAACCGTGTTGA AGGAGAACACGCCAGAGGAACTGCAACTTCCAGAAAACCCATACCTGCAAGCCAGCCCGGTAAGCGGCCA GAAGCAGTTCTCCTTCCACGAGGGCAGGAATGGAGTTCTAGAACGCAAAGACGGGCCCACAAAACTACCC CACGTGCTCAACTTCCAGAGGCACCCCGAGCTTCTGCAGCCCTACGACCCAGAAAAGAATGAGGTGAACT CAAAGAAGCATCACCCACCGGAGAGTCCCTGGCTGAGAAACGCGACAGAGGAAGGGACCAACTGTAAGAT GTCCCGGAACTGCTTTCCCAAACCCTGCGGTTCCCAGCCCAACAGCCCTACTCCAGAAGACTTAGCCAAG ACCAACATGACAGCTATCCGTCTCTGCAGCTCCGTGGTCATAGAAGACCCCCAAAAGCAGACCTCGGTGT GTGTGAATGTTCAGAGGTGTGCCAAGGAAGGGGTGGGAGAGGAGACCCTTTCCCCTCGACGGAAATCCCC AACAGCCCCCAGCCCACAGGCCTACTCCGAAACCAAGAAAAGCAGGCCAGATGTACTACCCTTTAGACGA CAAGATTCTGCAGGACCCATCCTGGATGGAGCTCGGTCACGAAGGTCCTCTTCGTCATCCACGACTCCCA CTTCAGCCACTTCCTTGTACAAATTTTTACTTGATGATCAGGAATGTGCCATCCACGCTGACAGTGTCAA CCGCCATGAAAACCGAAGGTATATTCCCTTCTTGCCAGGAACTGGGCGGGACATCGACACCTGCTCAATT CCTGGTGTGGACCAGTTAATTGAAAAGTTTGACCAAAAGCCTGGCCAGAGAAGAGGAAGGTCTGGGAGGA GAAACAGGATCAATGCAGACGACAGGAAAAGATCCCGAAGTGTAGATAGTGCCTTTCCATTTGGTCTCCA GGGAAATACAGAATACCTCACAGAGTTCAGCAGGAATCTGGGCAAATCTAGTGAACACCTCCTTCGGCCA TCCCAGGTCTTCCTGCAGAGGTCAGTGGCCCAGGAGCACCGTGGGAAGCACAGTCCCATCAGCACCCTTG CAAAACTGCAAGGTGCCCACCCCAAGCCTCCCCTGCAGAACAAAGACGGGAAAGTTCTGAACAAAGGAAG TCAGGAAAGCATAGGGGCAGGTGCCCCCTCCCTTCCAGCACAGAATAAAAAAGAGGAAGAAATCAAAATA GCCACCGCCACGTTGATGTTACAGAACCGGGCTGTGGCTGCCACATCTGATTCTGGTGCCAAGAAAATTT CAGTGAAGACATTTCCTTCCGACTCGAGTACTCAGGCCACGCCAGATCTCTTAAAGGGCCAGCAGGAGCT CACACAACAAACCAACGAGGAGACCGCCAAGCAGATCCTTTACAATTACCTCAGAGAAGGAGGCACAGAT AACGAGGATGCCACCAAAAGGAAGGTGAACCTGGTCTTTGAGAAAATCCAGACTTTGAAGTCCAGAGCAA CAGGGAGTGCCCAAGGAAACAATCAAGCTCCGAATTCTTCCTCTGAAGGCAACGATCTATTGGATCAGAA GAACAAATTGATCCTAGAAGTGTCTGAACTACAGCAGCAACTGCAGTTGGAGATGAAGAACCATCAGAAC ATCAAGGAGGAGAGAGAAAGGATGAGAGAGGATTTAGAAGAGCTGCGAGTCCGGCACCAGAGCCAAGTGG AGGAGACGGCCACCCTGCAGAGACGGCTGGAGGAAAGTGAAGAAGAGCTGAGGAAGAACTTGGAAGAGCT GTTCCAAGTGAAGATAGAGCGAGAACAGCACCAGACTGAGATCAGAGACCTGCAGGACCAGCTGTCAGAA ATGCACGACGAACTCGACAGCACCAAGCGATCCGAGGACCAGGAGAAGGGAGCCCTGATCGAGGAGCTCC TGCAGGCCAAGCAGGACCTTCAAGACCTGCTCATTGCCAAAGAGGAACAGGAAGATCTCTTGAGGAAGCG GGAGAGGGAGCTGACTGCCTTGAAGGGTGCCTTGAAGGAGGAGGTTTCCAGCCATGACCAGGAGATGGAC AAGCTGAAAGAACAGTACGACGCTGAGCTGCAGGCCCTCAGGGAGAGTGTGGAGGAAGCCACCAAGAATG TTGAGGTCCTGGCCAGCAGGAGCCACTCCTCAGAGCAGACCCAAGCAGAGGCTGACCTGCGTGAGAAAGC CCTGAAGGAGGAGAATGAGAAGCTGCAGGGAAGGATAGTGGAGCTGGAGCGCAGGGCGGCCCAGCTGCAG AGGCAGATGGAGGATGTGAAGGGTGACGAGGCCCAAGCAAAGGAGACACTCCGGAAGTGTGAGAGTGAAG TACAGCAGCTTGAGGAAGCCCTTGTGTGTGCCAGAAACGAAGAAAAGGAAGCCGCATGTGCCAGAACGGC TGTGGAGAGGGAGCTGGAGCAAGCTCAGAGAGAGCTGAGCCAGATCTCCCAAGAGCAGAAGGAACTCCTA GAGAAACTCCAGGAGGAGGTGGAGCAGAAGGAGCAGCTGAGGAAGCTGAAGAACGAGATGGAGAGTGAGC GATGGCACCTGGACAAGACCATCGAGAAGCTGCAGAAGGAGATGGCCGACATTGCTGAGGCGTCCCGCAC TTCCTCTTTGGAGCTGCAAAAGCAGCTGGATGAGTATAAGGAGAAGAATCGCCGGGAGCTTGCGGAAATG CAGACACAGCTGAAGGAGAAAAGCCTGGAGGTGGAAAAGGCCAGACTGGCAGCCTCAAAGATGCAGGATG AGTTGCGCCTCAAGGAGGAAGAGTTGCGGGACTACCAACGAGCTGAGGAGGAAGCGCTCACGAAACGGCA GCTTCTGGAGCAGTCACTGAAGGATCTGGAGTACCAGCTGGAGGCCAAGAGTCACCTCAAGGATGACCGA AGCCGACTGGTCAAGCAGATGGAGGACAAGGTGTCCCAATTGGAGATTGAGTTGGAAGAAGAAAGAACGA ACGCTGACTTGCTGTCTGAGAGGATCACTTGGAGCAGGGAACAGATGGAGCAGATGAGGAGTGAGTTACT TCAAGAGAAAGCTGCCAAGCAAGACTTAGAGTGTGACAAGATTTCCCTAGAGAGACAGAACAAAGACTTA AAGAGCCGGATTACCCACCTGGAAGGTTCCTACAGGTCCAGTAAGGAGGGCCTGGTGGTGCAAATGGAGG CCAGGATTGCAGAGCTGGAGGACCGGCTGGAGAACGAAGAGAGGGACCGGGCCAACCTGCAGCTCAACAA CCGAAGGCTGGAGCGGAAGGTGAAGGAGTTGGTGATGCAGGTGGATGATGAGCATCTGTCACTGACTGAC CAGAAGGACCAGCTGAGCTTGCGCCTGAAAGCCATGAAGCGCCAGGTGGAGGAGGCTGAAGAGGAGATCG ACAGACTGGAAAGTTCTAAGAAGAAGCTGCAGAGGGAGCTGGAGGAGCAGATAGGAGTGAACGAGCAGCT GCAGGGGCAGCTCAACTCTATGAAGAAGGGCTTAAGGCTTAAGACACTGTCTAGTAAAGTACTGGATGAC TCAGACGATGATGACCTCAGCAGTGATGCGGGAAGCCTCTATGAAGCCCCACTGAGCTATCCCTTTCCCA AAGACAGCACTATCACCAGCCAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108164
Insert Size	3879 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108164.1, NP_001101634.1
RefSeq Size	6480 bp
RefSeq ORF	3879 bp
Locus ID	315795

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