Dnmt1 (NM_053354) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200671 representing NM_053354 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGCACGAACAGCTCCAGCCCGAGTGCCTGCACTTGCCTCCCCGGCAGGCTCGCTCCCCGACCATG TCCGCAGGCGGCTCAAAGACTTGGAAAGAGATGGCTTAACAGAAAAGGAGTGTGTGAAAGAGAAATTGAA CTTACTGCATGAATTCCTGCAAACAGAAATAAAAAGCCAGCTATGCGACTTGGAAACCAAACTACATAAA GAGGAATTGTCTGAGGAAGGCTACCTGGCTAAAGTCAAGACCCTTCTAAATAAGGATTTGTGCCTGGAGA ACGGAACACTCTCTCTCACTCAGAAAGCCAACGGTTGTCCTGCCAACGGGAGTCGGCCAACCTGGAAAGC AGAAATGGCAGACTCAAACCGATCCCCAAGATCCAGGCCCAAGCCTCGGGGACCCAGGAGGAGCAAGTCG GACAGTGAGACCATGATTGAAGCTTCATCTAGTTCGGTGGCTACGAGGAGAACCACCAGGCAGACCACCA TCACGTCTCACTTCAAGGGTCCCGCTAAACGGAAACCCAAGGAAGACTCTGAAAAGGGAAACGCAAATGA ATCTGCTGCAGAGGAGAGAGACCAGGATAAGAAACGCAGAGTCGCAGGCACAGAGAGTCGTGCCAGTCGT GCCGGAGAGTCTGTGGAGAAACCTGAACGAGTAAGGCCAGGAACCCAGCTGTGTCAGGAAGAGCAAGGTG AACAGGAAGATGACAGGAGACCTCGACGTCAAACCAGAGAGCTAGCATCAAGGCGGAAATCAAGGGAGGA CCCAGACAGAGAAGCAAGGCCAGGAACTCACTTGGATGTGGACGATGACGATGAAAAGGACAAAAGAAGT TCCAGGCCCAGAAGCCAGCCCAGAGATCTAGCCACCAAACGGAGACCCAAGGAAGAAGTAGAGCAGATCA CTCCAGAGCCTCCCGAGGGCAAAGATGAGGACGAAAGGGAGGAGAAGAGACGAAAAACGACACGTAAAAA GCCGGAACCACTCAGCATTCCCGTACAGAGCAGAGTGGAGAGAAAAGCCTCTCAGGGCAAAGCAAGTGCA ATCCCAAAGCTCAACCCGCCACAGTGCCCTGAGTGTGGCCAGTACCTAGATGACCCTGACCTGAAGTACC AGCAGCACCCTGTGGATGCTGTGGATGAACCCCAGATGCTGACCAATGAGGCACTGTCCGTCTTTGACTC CAACTCGTCTTGGTTTGAGACCTATGATTCCTCTCCCATGCATAAGTTCACTTTCTTCAGTGTGTATTGC AGTCGCGGTCACTTGTGTCCTGTCGACACTGGTCTCATTGAGAAGAACGTTGAACTCTACTTTTCTGGTG TTGCCAAAGCAATTCATGAAGAAAATCCGTCTGTGGAAGGTGGTGTTAATGGCAAGAACCTGGGGCCAAT CAATCAGTGGTGGATCAGTGGCTTTGACGGTGGCGAGAAGGCGCTCATTGGCTTTTCTACCGCATTTGCT GAATACTTTCTGATGGAGCCCAGCCCAGAGTATGCACCAATATTTGGGCTGATGCAGGAGAAAATTTACA TCAGCAAGATTGTGGTCGAGTTCTTGCAGAGCAATCCTGACGCTGTCTATGAAGACTTGATCAATAAGAT CGAGACCACTGTTCCTCCTTCTGCCATCAATGTGAACCGGTTCACCGAGGACTCCCTCCTACGCCATGCC CAGTTTGTGGTGAGCCAGGTAGAAAGTTACGACGACGCTAAGGACGATGATGAGACGCCCATCTTCTTGT CTCCCTGTATGAGGTCCCTGATCCATTTGGCTGGTGTCTCGCTGGGACAGAGGCGAGCAACAAGGCGCAC GGTCATCAATTCTGCCAAAGTGAAGCGCAAGGGGCCCACAAAAGCCACCACCACCAAGCTGGTCTATCAG ATCTTCGACACTTTCTTCTCGGAGCAGATCGAGAAGGATGACAAGGAGGACAAAGAGAACACCATGAAGC GCCGCCGGTGTGGTGTCTGTGAGGTCTGTCAACAGCCTGAGTGTGGGAAGTGCAAGGCGTGCAAAGACAT GGTTAAGTTTGGTGGCACCGGACGGAGTAAACAGGCTTGCCTCAAGAGGAGGTGTCCTAACTTGGCCGTA AAGGAGGCAGATGAAGACGAAGAAGCTGATGATGATATACCAGAGTTGCCGTCACCCAAAAAGTTGCACC AAGGGAAGAAGAAGAAACAGAATAAGGATCGCATCTCCTGGCTCGGGGAGCCTGTGAAGATCGAAGAGAA CAGAACTTACTATTGGAAGGTGAGCATCGACGAAGAGACTCTGGAGGTGGGCGACTGCGTCTCGGTCATT CCGGATGATCCCTCCAAACCACTGTATCTAGCCAGGGTCACAGCACTGTGGGAAGACAAGAACGGGCAGA TGTTCCATGCACACTGGTTCTGCGCGGGGACAGACACGGTCCTCGGAGCCACCTCTGACCCTCTGGAGCT GTTCCTTGTAGGCGAGTGTGAAAACATGCAACTTTCCTACATCCACAGCAAGGTCAAGGTCATCTACAGA GGGCCTTCCCCAAACTGGGCCATGGAGGGAGGCATGGACCCTGAGGCCATGTTGCCGGGGGCTGAGGATG GGAAGACCTACTTCTACCAGTTCTGGTACAGCCAGGACTACGCAAGGTTTGAGTCCCCACCCAAGACCCA GCCGACGGAGGACAATAAGCACAAGTTCTGCCTATCTTGTATCCGGCTGGCTGAGCTGAGACAAAAAGAA ATGCCCAAAGTCCTGGAACAACTTGAGGAGGTGGACGGCCGGGTCTACTGCAGTTCTATCACCAAGAACG GTGTTGTCTACCGACTGGGTGACAGTGTGTACCTGCCTCCTGAGGCCTTTACTTTCAACATCAAAATGGC CAGCCCCATGAAACGCTCTAAGAGGGACCCTGTGAATGAGAACCTGTACCCCGAGCATTACCGCAAGTAT TCCGACTACATCAAAGGAAGCAACCTGGACGCCCCTGAACCCTATCGGATTGGTCGGATAAAAGAGATCC ACTGTGGCAAGAAGAAAGGTGGCAAGGTCAATGAGGCAGACATCAAGATCAGGCTCTACAAGTTCTATAG GCCTGAGAACACCCACAAGTCCATCCAGGCAACCTACCACGCCGACATCAACCTGCTCTACTGGAGCGAT GAGGAAGCCGTGGTGGACTTCAGCGACGTGCAAGGCCGCTGCACCGTGGAGTACGGAGAGGACCTGCTTG AGAGCATCCAGGATTACTCACAGGGCGGCCCCGACCGCTTCTACTTTCTCGAGGCCTACAATTCAAAGAC TAAAAGCTTTGAAGACCCTCCGAACCATGCCCGCAGCCCTGGGAACAAAGGCAAAGGAAAGGGGAAAGGG AAAGGGAAGGGAAAACCTCAGGTATCAGAGCCCAAAGAGCCCGAGGCAGCCATCAAACTGCCTAAGCTGC GGACCCTGGATGTGTTTTCTGGCTGTGGAGGATTAACAGAAGGATTCCACCAAGCAGGCATCTCGGAGAC GCTGTGGGCCATCGAGATGTGGGAACCAGCAGCCCAGGCATTTCGGCTGAACAACCCTGGCACCACGGTG TTCACAGAGGACTGCAACGTGCTTCTCAAGCTGGTCATGGCTGGTGAGGTGACCAACTCTCTGGGCCAGA GGCTGCCACAGAAGGGCGATGTGGAGATGCTGTGTGGTGGGCCGCCCTGCCAGGGCTTCAGTGGCATGAA CCGTTTCAACTCTCGTACTTACTCCAAGTTCAAAAACTCCCTCGTGGTCTCCTTCCTCAGCTACTGTGAC TACTACCGGCCTCGGTTCTTCCTCTTGGAGAATGTCAGGAACTTCGTGTCCTTCAGGCGCTCCATGGTGC TGAAGCTCACACTGCGCTGCCTGGTCCGCATGGGCTACCAGTGCACCTTCGGTGTGCTCCAGGCTGGACA GTATGGTGTGGCCCAGACACGAAGGAGGGCCATCATCTTGGCTGCAGCCCCTGGAGAGAAGCTGCCTCTG TTCCCAGAACCTCTACACGTGTTTGCCCCCCGTGCCTGCCAGCTGAGTGTTGTGGTGGACGACAAGAAGT TCGTTAGCAACATTACAAGGCTAAGCTCGGGTCCCTTCCGAACCATCACGGTGCGAGATACCATGTCTGA CCTCCCTGAGATCCAGAATGGAGCCTCAGCCCCCGAGATCTCCTACAATGGAGAGCCACAGTCCTGGTTC CAGAGGCAGCTGCGAGGGTCGCACTACCAGCCCATCCTCAGGGACCATATCTGCAAGGACATGAGCGCGC TGGTGGCTGCCCGTATGCGGCACATCCCGCTGTCCCCAGGATCGGATTGGCGTGACCTGCCCAACATACA AGTGCGGCTGAGAGATGGCGTCATAACCAATAAACTTCGCTACACTTTCCACGACACGAAGAACGGCTGC AGCAGCACCGGGGCCCTGCGTGGGGTCTGCTCCTGTGCGGAAGGCAAGACCTGCGACCCTGCTTCCAGAC AATTCAACACGCTCATCCCCTGGTGCCTGCCGCACACTGGGAACCGGCACAACCACTGGGCTGGCCTCTA CGGGCGTCTGGAGTGGGATGGCTTCTTCAGTACCACCGTCACCAACCCTGAGCCCATGGGCAAGCAAGGC CGGGTGCTCCATCCCGAGCAGCACCGGGTCGTGAGTGTGCGGGAATGTGCTCGCTCCCAAGGCTTTCCAG ATACCTACCGGTTATTCGGCAACATCCTGGACAGACACCGGCAGGTGGGGAACGCTGTGCCACCACCACT GGCCAAAGCCATCGGCCTGGAGATTAAGCTCTGTCTGCTGGCCAGCGCTCAGGAGAGTGCCTCAGCTGCA GTTAAAGGAAAGGAGGAGACTACTACCGAGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053354
Insert Size	4866 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053354.3, NP_445806.3
RefSeq Size	5917 bp
RefSeq ORF	4866 bp
Locus ID	84350
Gene Summary	methylates genomic DNA; involved in processes of gene inactivation, chromatin organization, X chromosome inactivation and genomic imprinting [RGD, Feb 2006]

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