Sh3tc1 (NM_001107226) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200825 representing NM_001107226 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCTTCAGGGAGGAGGAGGCGACTTCTGAGGATCTGTCCACCATGGGGCAGGGCCCTGAGGAGC CTCCAGGAGGCTGTAGCCTGGAAGACAAGATCCAGAGTATATCCATGGGCTTACCTACTAGTCAGGAGAG AACAGGGCCTGAGGAGGCTGAGGCAACAACAGTCAGAGGTGACGTTGCTCCTCCCTGTGGCATGGTTGGG CAAGCTGCTGAACCCCCTTCCAATCAAATGGCAGTGTATCCGACAGACCTCACCCTGCAATTGTTGGCTG TGAGGCGGAAGAGTGGACTGCGGAGCCCCAGCCTACAGCAGACCCTGAGAAGCCGACTCCGCCTGCTGGA GAATGACAGCCGGGAAGTGGCCCGAGTACTTGGAGAACTGTCAGCCAGGCTGCTGTCCATCCACAGTGAC CAGGACCGGATCGTGGTGACGTTTAAGACTTTTGAAGAAATCTGGAAGTTTTCCACCTATCATGCTCTTG GCTTCACACATCACTGCCTGGAGAACCTGCTGGTGGACCAGACCTTCTGGCTGCTTTCCCCCAGTGAGGA GGAGGAGACTGCCATCCTCATATATGTAAACAAGGATGCCTTGAAGCAGACACACAAGAGCCTTCTCGCA CAAGAAGGGTCCTTCTTCGTTCTGTGTCCCGACCACTGTGTGAGGAGGACAAAGGAACCCCAAGGTGCAG GGAAGGGCCCCCAGACCCTCAGACGGGCTTCCGGGGTCACTCTGGGAGTACCAGTTGTGGAAACAGTCTC CTCATCCCCCACACCCAGCATGTCCTCCAAAGCAGAAGCAGTAGAGGCTGAGCCAGAGCCCTTGACTCCA TTTCACCAGTGGACTCTCAGGGTCCCATGGGATCCCATCGATGAATCCATGAGTGGACCTGGGACGCCTG ATGCCCAGTTCATGAGGTTTGGCCTGGCCTCTGCAATAGCTGACTTCCAGGGCTCAGGCCCGGAAGAGAT GTCCTTCAGCGTTGGTGATGTCATCGAGATCGTCGGTGCCCAGGTGCCTGGCCTGCCCTGGTGTTTGGGC CAGCACATGGCCTCTGGCCAGGTGGGCTTTGTGAGGACTGGCCTTGTCCGCATGCAGGGCCCGGCATCTG ACCTGGAAAGTTCAATTTTCCTCAGCGAGGAAGAAAGGTCATTCTTCAGAAGTGAGGCTCACTTCTCAGA CGAGGATGCCCGGCGGCTCCTGTGCAGGACATCTGGCACAGATGTGTGTACCACATGCAGCCTGGACTGG TTAGAAGAAGCTGAGGGAGAACAGCTGGAGCAACAAGAAATGTCTCTGCCTCACCTGAACCCAGAGCCAC ATGAGAGTCTGCACATGGTAAAGAACGTTTTAGAGAAGTGCAAGGCCTGCCCTGACCACCCTGAAGACCC TGTATCCTGGAGCCTTGGAGCGGTGTCCAGCAGAGTGAGTTCACCGGACCTTGAGGAGCCCCCCTTCTGC CTGGATCCTGACGATGACTGGACAGACCCTGAGCCTCCGGACTCACTGCTGCAGGTCCTGAGTGCCCCTG GTTATAGGGCCCAGTTCCGGAGCCTGTATGATATCTCACTTCCCTGCCTGAGCACCGCACTCTATAGCTT TGATGATGAAGAAGAGTTGGCTGAACGCTTGGCACAGGCCCGAGGAGTTGCCAAGAAAGTCGATTTGGGC ATGGCCCTGGCAAGGCTCTGCCTCCTGCTGGGGCGGCTGTGTGCCCGAAAGCTCAAACTATCCCAGGCCC GGGTGTACTTTGAAGAGGCCCTGGGGGCTCTGGAGGGAAGTTTTGGGGACCTCTCCCTGGTGGCGGCCGT GTATAGCAGCTTGGCTACTGTGTACCTGAAGCAGAAGAACGGGGAGAAGTGTGTGCAGCTGGCACCCAAA GCCGCAGCGCTGCTCCTGGGAACGCCCGGCCACTCCTGCAGCACAGATGCCGAGCTCCTGAAGTACGCAC TGCGCAGGGTCGTCTGTGGCCTCAGCCCACAGGCCGAGGCCCGGGCCTGCTTCCTGCTGGCCCGGCACTA CACCCACCTCAAACAGCCTGAGGAGGCACTGCCATACTTGGAGAGACTGCTTCGCCTCAACAGGGATGTG GGGACCCCGCAGGCCTCGTGGCCTGAAGACTGTTACCTGCTTCTGGCGGACATCTATGGCCGGAAGTGCC TGCCGCACCTGGCATTGAGCTGCCTCAAGGTGTCCTCACTGTGGACAAGATGCTCTCTGGCTGGCTCCTT GAGGAGTGTGGACCTGGTACTCCAGAATGCTCCTGGGCCAAACAGCCAAAGGAGCACAGGTCATAGCCTT CCCTCCCAGATTGCTCACTACCTCAGACAGGCATTGGCCTCTCTGCCTCCCGGCACGGGACAGACACTGC GAGGGCCACTCTATGCCAGCCTGGCTCAATTGTACAGTTACCACCAGCAGTACGGCCAGGCCATAGCCTT CATGTCTCAGGCTGCAGAAGCTGACACTGCAGCTGGGGTCCACCCTGTCGTGGACCGCTTGGTGGCGCTC GCCTGGCTGCACCTGCTTTGTGGGAAAAGCTTAGTAGCCATGGACATCCTGAAGTGCGTCTCAGACGCGG CCGTGGCCAATAAGGACCAGGAATGTGTTATGATGAACATGGTGGCCATGGCCCTGAAGAGGATGGGCAG GACCCGGCAGGCTGCTGAAGGCTACTTCCGAGCTCTGCACATGGCCTACAGCCAGGGCCACCTGCAGAGC CAGGCAGTGGTCCTAGCTAACTTTGGGGCCCTGTGCCTGCAAGCTGGCGCACGCAGCTTGGCCCAGCACT ACCTGCGGGAGGCCGTGGGGCTCTTCTCCCAGCTGCCCAGTGGCGTGTGTGGCCGTGACTTCACCCAGGT GCTCCTGTGGTTGGGCCAACTGTGTACGCGCCGGACCCTCCCCCAGCAGGCCAAGTGCTACTATGAGTGG GCCTTTCTGGTGGCTGTGGAGACGGACCATCTGGAGAGTCAGTTACAGGCGGTGCAAAAGCTGTGCCACT TCTACAGTAAGGTCATGCCCAACGAGGTGCGATGTGTCATCTACCATGAGTTCCAACTGGCTCTGGCACG CAAGACCGCTGACAAGGTGCTGGAGGGGCAGCTCCTGGAGGCCATCAGTCAGCTCTACCTGTCGCTGGGC ACTGAGCGGGCCTACAAGTCGGCTCTGGATTACACTAAACGGAGCCTGGGGATTTTCATCGACCTGCAGC AGAAGGAGAAGGAGGCACGCGCCTGGCTGCAGGCAGGGAAGATCTACTATATCCTCCGTCAGAATGAGCT GGTGGACTTGTACATCCAGGTGGCACAGAATGCAGCCCTGTATACCGGGGACCCTAAGCTGGGGCTGGAA CTGTTTGAGGCAGCTGGGGACATCTTCTTCAATGGGACCTGGGAGCGGGAGAAAGCTGTGTCCTTCTACC GGGACCGGGCACTGCCCCTGGCTGTGACTGTAGGAGACCAAGAAGTGGAGCTGCGCCTGTGCAACAAGCT GGTGGCCCTGCTGTCTGCACTGGAGGCACCCCAGGAGGGCCTAGAGTTCGCCCATGAGGCCCTAGCACTC AGCATCACCCTAGGTGACCGGCTGAATGAGCGAGTGGCCTACCACAGGCTAGCCACCCTGCACCACCGGC TGGGCCACGGGGAACTGGCCGAGCACTTCTTCCTGAAGGCTCTGTCGCTCTGCACCTCGCCCCTGGAATT TGATGAGGAGACCCTGTACTATGTGAAGGTCTACCTGGTACTGGGTGACATCATCTTCTATGAGCTGAAG GACCCCTTCGATGCAGCTGGGTATTACCAGCTGGCTCTGGCAGCAGCCGTGGACCTGGGCCACAAGAAGG CTCAGTTGAAGATCTATACACGCCTGGCTACCATCTACCACAACTTCCTCATGGACCGAGAAATGTCCCT CTTCTTCTACCAAAAAGCAAGGACCTTTGCCTCAGAACTGAATCTCCGCAGGACCAACCTGGTACCCCAG CACTTCTGTAGCCGGGCACCCTGGTTGGCCCCTGGCTACCCATCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107226
Insert Size	4038 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107226.1, NP_001100696.1
RefSeq Size	4220 bp
RefSeq ORF	4038 bp
Locus ID	305441

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