L1cam (NM_017345) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hsas; Hyd; N-CAM L1; NCAML1; NgCAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN200937 representing NM_017345 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCATGATGCTGTGGTACGTGTTGCCTCTTCTCCTCTGCAGCCCCTGCCTGCTCATACAGATTCCTG ATGAATATAAAGGACACCATGTACTGGAGCCACCTGTCATCACAGAACAGTCTCCACGGCGCCTGGTTGT CTTCCCAACAGATGACATAAGCCTCAAATGTGAAGCCAGAGGCAGACCCCAAGTGGAGTTCCGCTGGACG AAAGATGGCATCCACTTCAAACCTAAGGAAGAATTGGGTGTAGTGGTACACGAGGCACCCTATTCTGGCT CCTTCACCATCGAAGGCAACAACAGCTTTGCCCAGAGGTTTCAGGGCATCTATCGCTGCTATGCCAGCAA TAATCTAGGAACTGCCATGTCGCATGAGATCCAGCTCGTGGCTGAGGGTGCCCCCAAATGGCCGAAGGAG ACTGTAAAACCCGTGGAAGTGGAGGAAGGAGAATCAGTAGTTCTACCTTGCAATCCTCCACCCAGTGCAG CCCCACTTAGGATCTACTGGATGAACAGCAAGATTTTGCACATCAAACAAGATGAGCGGGTGTCCATGGG CCAGAACGGAGACCTATATTTTGCCAATGTGCTTACCTCAGACAATCATTCAGACTACATCTGCAATGCC CACTTCCCTGGCACCCGGACCATCATTCAAAAGGAACCTATTGACCTCCGGGTCAAGCCCACCAACAGCA TGATTGACCGGAAGCCACGCCTGCTCTTCCCCACAAACTCCAGCAGTCACCTCGTGGCCTTGCAGGGCCA GTCATTAATCCTGGAGTGCATTGCTGAGGGATTCCCTACACCCACCATCAAGTGGCTGCACCCCAGTGAC CCTATGCCAACAGACCGTGTTATCTACCAGAACCATAACAAGACACTGCAGCTCCTCAATGTGGGCGAGG AAGATGATGGCGAGTATACCTGCCTTGCTGAGAACTCACTGGGCAGTGCTCGGCATGCCTACTATGTCAC TGTGGAAGCTGCCCCATACTGGCTGCAGAAGCCCCAGAGTCATTTGTATGGGCCAGGAGAGACTGCCCGC CTAGACTGCCAAGTCCAGGGCAGGCCCCAACCAGAGGTCACCTGGAGAATCAACGGAATGTCTATAGAGA AGGTGAACAAGGACCAGAAGTACCGGATTGAGCAGGGGTCTTTGATCCTGAGTAATGTGCAACCAAGTGA CACAATGGTGACCCAGTGTGAAGCTCGCAACCAGCATGGGCTCCTACTAGCCAATGCCTATATCTATGTT GTCCAGCTGCCAGCCAGGATCCTAACAAAAGACAATCAGACATACATGGCAGTAGAGGGCAGTACTGCTT ACTTGCTGTGCAAAGCCTTTGGAGCTCCTGTTCCCAGTGTCCAGTGGCTGGATGAGGAAGGAACCACAGT GCTTCAGGATGAAAGATTTTTCCCCTATGCCAATGGACACCTGGGCATCAGAGATCTCCAGGCCAATGAC ACTGGACGCTATTTCTGCCAGGCTGCCAATGACCAGAACAATGTGACCATTTTGGCTAACCTACAGGTTA AAGAAGCAACCCAGATCACACAAGGACCCCGGAGCACAATTGAGAAGAAAGGTGCAAGGGTGACATTCAC GTGCCAGGCCTCCTTTGACCCCTCTTTACAAGCCAGCATCACTTGGCGTGGAGATGGGAGAGACCTCCAG GAACGTGGAGACAGTGACAAGTATTTCATAGAAGATGGGCAACTTGTCATCAAGAGCCTGGACTACAGTG ACCAGGGAGACTACAGTTGTGTGGCCAGCACTGAACTGGATGAGGTGGAGAGCAGGGCACAACTCTTAGT GGTGGGAAGCCCTGGGCCAGTGCCTCACCTGGAGCTGTCCGACCGCCACTTGCTGAAGCAGAGCCAGGTG CACTTGTCTTGGAGCCCTGCTGAAGACCACAACTCTCCCATTGAGAAATATGACATTGAATTTGAGGACA AGGAAATGGCTCCTGAGAAATGGTTCAGTCTAGGCAAGGTGCCAGGAAATCAGACCTCTACTACCCTCAA GCTGTCCCCCTATGTCCACTACACCTTTCGGGTCACTGCCATTAACAAATATGGTCCTGGAGAACCCAGC CCTGTCTCTGAGACTGTAGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAGAGGGGAAG GAAATGAGACCAACAATATGGTCATCACATGGAAGCCCCTTCGGTGGATGGATTGGAATGCCCCCCAGAT TCAGTACCGTGTACAGTGGCGACCACTGGGCAAACAAGAGACCTGGAAGGAACAGACCGTGAGCGACCCC TTCCTGGTGGTGTCTAACACTTCCACATTTGTGCCTTATGAGATCAAAGTCCAGGCAGTGAACAACCAGG GGAAGGGCCCTGAGCCCCAGGTCACCATTGGCTATTCAGGGGAAGACTACCCCCAGGTGAGCCCTGAGCT TGAAGACATCACAATCTTCAACTCAAGCACTGTGCTGGTCAGGTGGAGGCCTGTGGACTTGGCCCAGGTT AAGGGCCACCTCAGGGGATACAATGTAACGTACTGGTGGAAGGGCAGTCAGAGAAAGCACAGCAAGAGGC ATGTCCACAAAAGTCACATGGTGGTACCTGCGAACACCACCAGTGCCATCCTCAGTGGTTTGCGTCCTTA CAGCTCTTATCATGTAGAGGTACAGGCCTTTAATGGGCGGGGCTTAGGGCCTGCAAGTGAATGGACCTTC AGCACCCCAGAGGGAGTGCCTGGCCACCCTGAGGCATTACATCTGGAGTGCCAGTCGGACACTAGCCTGC TACTGCACTGGCAGCCACCACTCAGCCACAATGGAGTGCTCACTGGCTACCTGCTCTCTTACCATCCCTT GGATGGGGAAAGCAAAGAGCAGTTGTTCTTCAACCTTTCGGACCCAGAGCTCCGGACTCATAATCTCACC AACCTCAACCCTGATCTACAGTACCGCTTCCAGCTTCAGGCCACCACCCATCAGGGTCCTGGTGAGGCCA TTGTGCGTGAAGGAGGCACTATGGCCCTATTTGGCAAGCCAGATTTTGGCAACATTTCAGTCACAGCAGG TGAAAACTACAGTGTGGTCTCCTGGGTCCCTCGGGAGGGCCAGTGCAATTTCAGGTTCCACATCCTGTTC AAAGCCTTGCCAGAAGGGAAAGTGAGCCCTGATCACCAGCCTCAGCCTCAATATGTGAGCTACAATCAGA GCTCCTACACACAGTGGGACCTACAGCCTGACACCAAATATGAGATCCACCTGATGAGGGAGAAGGTCCT CTTGCACCATCTGGCTGTGAAGACTAATGGCACTGGCCCCGTGCGAGTTTCTACTACAGGTAGCTTTGCC TCCGAGGGCTGGTTCATCGCCTTTGTCAGTGCTATCATTCTCTTGCTCCTCATCCTGCTCATCCTCTGCT TCATCAAACGCAGCAAGGGCGGCAAATATTCAGTGAAGGACAAGGAGGACACTCAGGTAGATTCCGAGGC CCGGCCCATGAAAGACGAGACCTTCGGCGAGTACAGTGACAATGAAGAGAAGGCCTTCGGCAGCAGCCAG CCATCTCTCAATGGAGACATCAAACCCCTAGGCAGTGATGACAGTCTGGCTGATTATGGGGGCAGTGTGG ATGTCCAGTTCAATGAGGATGGCTCTTTCATCGGCCAATACAGTGGCAAAAAAGAGAAGGAGGCAGCGGG AGGCAATGACAGCTCAGGGGCTACCTCTCCTATCAATCCTGCAGTAGCCCTAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017345
Insert Size	3768 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017345.1, NP_059041.1
RefSeq Size	5093 bp
RefSeq ORF	3768 bp
Locus ID	50687
UniProt ID	Q05695
Gene Summary	member of the Ig superfamily of cell adhesion molecules; involved in neuronal cell development; neural cell adhesion molecule expressed in brain; isoform L1cs is a likely alternative splice variant expressed in the peripheral nervous system [RGD, Oct 2007]

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