Washc1 (NM_001127390) Rat Untagged Clone
CAT＃: RN201376
Wash (untagged ORF) - Rat WAS protein family homolog 2 (Wash2), (10 ug)
CNY 6180.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | ORF19; Wash; Wash1; Wash2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN201376 representing NM_001127390
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCGGTGAAGACCCAGCACTCCTTGGCAGGCCAGGTTTATGCGGTGCCCCTCATCCAGCCAGACC TGCGTCGAGAGGAGGCAATCCAGCAAGTGGCAGACGCCCTGCAGTACTTACAGAACATCTCCGGAGACAT CTTCAGCAGGATCTCCCAGCGAGTAGAGCTGAGCCGACGCCAGCTGCAGGCCATTGGGGAGAGGGTCTCC TTGGCCCAGGCTAAGATAGAGAAGATCAAGGGCAGTAAGAAGGCCATCAAGGTCTTTTCCAGTGCCAAGT ACCCAGCCCCGGAGCACCTGCAGGAGTATAACTCCGTCTTTACTGGAGCCCTGGACCCTGGTCTGCAGAG AAGACCCCGGTACAGGATCCAGAGCAAGCACCGCCCACTGGATGAGCGGGCTCTGCAGGAGAAGCTGAAA TACTTTCCCGTGTGTGTGAGCACCAAGTCGGAGCCTGAGGATGAGGCTGAGGAGGGACTTGGGGGTCTTC CCAGCAACATTAGCTCCATCAGCTCCCTGCTGCTCTTCAACACCACAGAAAACCTGTATAAGAAGTATGT TTTCCTGGACCCTCTGGCTGGTGCAGTAACGAAAACACACACCATGCTGGGCACAGAGGAGGAGAAGTTG TTCGATGCTCCTCTGTCCATCAGCAAGAGAGAGCAGCTGGAGCAGCCAGCTCCAGAAAACTACTTCTATG TGCCAGGCCTGGGCCAGGTTCCTGAGATCGATGTGCCATCCTACCTACCCGACTTGCCTGGCGTCGCAGA CGACCTGATGTACAGTGCGGATTTGGGTCCCGGTATTGCCCCGTCCGCTCCTGGTGCTATTCCAGAGCTG CCTGCCTTCCATACGGAGGTGGCGGAACCTTTCCAGCCAGAACGGGAGGATGGAGCGCTGTTGGCACCAC CACCGCCTCCTCCTCCTCCTCCACCGCCACCTCCCCCAGCTCCCACAGCGGTGGTCAGTGCGCCCCAACC ACCGATGTCCCCTGATGTGGTAACCGTGACTGGCAAGGTTGCCAGGGAAGAGGACTCTGGCAGCGGTGAG GCCCACTCAGCCTCTGTTCAGGGAGCCCCTAAGGAGGTGGTTGACCCCTCCAGCGGCCGGGCCACTCTGC TTGAGTCCATCCGCCAAGCCGGGGGCATTGGCAAGGCCAAGCTGCGCAGTGTCAAGGAACGCAAGCTTGA GAAGAAGAAACAGAAGGAGCAGGAGCAAGTGAGAGCTACCAGCCAAGGCGGGGACTTGATGTCAGATCTC TTCAACAAACTCGTCATGAGGCGCAAAGGTATCTCTGGAAAAGGACCTGGCACTGGCACCAGTGAGGGGC CTGGTGGAGCCTTCAGTCGAATGTCAGACTCCATTCCACCTCTGCCGCCCCCACAGCAGCCAGCAGGAGA CGAGGACGAGGATGACTGGGAGTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001127390 |
| Insert Size | 1428 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001127390.1, NP_001120862.1 |
| RefSeq Size | 1885 bp |
| RefSeq ORF | 1428 bp |
| Locus ID | 367328 |
| UniProt ID | B2RYF7 |
| Gene Summary | Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR201376 | Wash (Myc-DDK-tagged ORF) - Rat WAS protein family homolog 2 (Wash2), (10 ug) |
CNY 6180.00 |
|
| RR201376L3 | Lenti ORF clone of Wash (Myc-DDK-tagged ORF) - Rat WAS protein family homolog 2 (Wash2), (10 ug) |
CNY 7980.00 |
|
| RR201376L4 | Lenti ORF clone of Wash (mGFP-tagged ORF) - Rat WAS protein family homolog 2 (Wash2), (10 ug) |
CNY 8840.00 |
