Kntc1 (NM_001107140) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202247 representing NM_001107140 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAACAATATCGAGCTGCTAACCAGCGATGACACGGGAAGTGGATTCCTCAATGTTGGCTCACGAA AAGAAAGCGTGACTGCACTGTACCAGGTGGACTTGCTTGGGAAGATCTCCTCTGAGAAGACCTCACTGAG TCCCAAGATCCAAGCATGCAGCTTAAGCGATGGGTTCATAATCGTGGCAGACCAGTCAGTGATACTGCTT GACAGTACATGCAGATCCCTTCAGCTGTTTCTTATATTCGACACTGACGTGGACGTGGTTGGCCTGTGTC CAGAAGGAAAGTTTCTCTTTGTGGGGGAGAGAAGCGGCAACTTTCATCTTATCTATGTAACATCGAAGCG AACTCTGTTCACCAAGGCCTTTGTGGAGAAAGCCGAGGATGAGCGTACTTACCGGAGTCTCGTTATTGAG AAAGATGGTTCAAATGAAGGTACCTATTATATGTTGCTTCTGACGAACAACGGATTTTTTTACATTACAA ACCTCCAGCTTTCACAAATTGAACAAGCAATTGAGAACACAGACTTGGATTCAGCCAAGAAGGGAACTGG TAGCTGTGCGTTCTCAAAATGGGAACCAGATTCTACCAAGAAAGACATGTCTCTTAAGAACTTTGTGGAC ACGGACATTATTAGAGGTGCAAAGTCATTTCAGCTGATCGACAATCTGCTTTTTGTGCTTGATACTGATA ATGTGCTGAGCTTGTGGGATGCTTATACTCTCACCCCGGTGTGGAACTGGCCTTCTCTTCCCATAGAGCA GTTTCTCCTCACGACAGAAGCGGACTCCCCCTCTTCAGTTACCTGCCTATGTGAAGACTCTGTGTCTAAC TTAGAACTTAGGTACCTCACAGAGGTGTTACCAGAAAACAGACTGAGCCGGCTACTTCACAAGCACAGAT TTGCCGATGCCGAGAGCTTTGCCATTCAGTTTGGACTGGACGTTGAGCTTGTTTATAAGGTCAAATCAAA TGATATATTGGAGAAGCTGGCTTTGATCTCAGACAAGAGCGAGCAGTCCGAATGGCAGCAACTTGTAGAT GAAGCCAAAGAAAACCTATGCAAAATCCAGGACGATGACTTCGTGGTGAATTTCTGCCTCAAGGCCCAAT GGGTCACCTATGAAACCACCCAGGAAATGCTCAGTTATGCCAAAACCAGGGTGCTAAAAGCTCATGCGAA GTTAACTACATTTTATGGAGCATTTGGACCAGAGAAATTCAGTGGTAGCGCTTGGGTTGAATTCCTGAAT AATGAAGATGACCTCAAGGATATTTTTGTGCAGCTAAGTGAGGGAAACTTCGATTGTGCTCAGTATCTTT GGCTTAGACATCGGGCTGACTTTGAAAGCAAGTTCGACGTGAAAATGCTGGAGAATTTGCTGAACTCAAT ATCTACACAAATCCCCGTGGAAAAACTCTGTTCCTGGTTTAAAAACGACGTGATTCCCTTTGTGAGGAGG ATTGTGCCCGAAGGACAAAACATTCTTGCAAAATGGCTGGAACAAGCCTCGAGGAACCTAGAGCTAACGG ATAAGGCAAACTGGCCAGAAAATGGACTTCAACTGGCAGAGGTCTTCTTCACTGCAGAAAAAACTGACAA ATTTGGATTCTCGTCCTCTTGGCACTGGATCTCCTTGGATTATCAAAACACGGAGGAAGTCCAACAGTTG CGGACACTGGTGAGCAAGTTGCAGGAGCTGATCATCCTGCATAGGAAATACAACTGCAGGCTGGCCCTCT CTGAGTTTGAGAAGGAAAATGCCACCACTGTGGTGTTCCGCATGTTCGACAGGGTCTCGGCCCCAGAGCT GATCCCTTCTGTCTTAGAGAAGTTCGTGAGAGTTTACATTCGGGAACAGAATTTGCAAGAGGAGGAACTT CTTTTGCTCTATATAGAGGACTTACTAAAGAGGTGCAGCTCTAAGTCAATGACACTGTTCGACACCGCGT GGGAAGCGAAGGCCATGGCCGTGATAAGATGCTTGTCTGACACAGATCTCATCTTCGACGCTGTGCTGAA GATAATGTACAAGGCCGTGGTTCCCTGGAGTGCCGCTGTGGAGCAGCTGGTGAGACAGCATCTGGAAATG GACCACCCCAAAGTCAAGTTATTACAGGAAAGCTACAGACTCATGGAGATGAAGAAACTCTTGCGAGGCT ATGGGATAAGAGAGGTGAATCTCTTGAACAAGGAAATAATGAGAGTGGTCCGCTACATTCTCAAGCAAGA CATCCCGTCGTCTTTAGAAGATGCTCTGAAGGTGGCCCAAGGGTACAGGCTGTCCGACGACGAAATCTAC AGTCTAAGGGTCATTGATCTGATTGACCGAGATCAGGGGGAAGATTGTCTCCTGCTCCTGAGGTCTCTGC CTCCCAGTGAAGCTGAGAAAACCGCAGAAAGAGTCATCATCTGGGCTCGACTGGCACTGCAAGAAGAGCC TGACGGTTCTGAAGAGGACAAGGCCTGGAGAATATCTGTGGCGAAGACATCGATGGATATTCTCAAAATC CTGTGTGATATTCAGAAAGACAATCTGCAGAAGAAGGATGACGCTGAAGAATTCCTGAAGCGATTTAAAA TGGTTGCTAGTTTACAGGAGAACTTCGAGGTCTTCCTTCCATTTGAGGATTACAGCGACACTGGCTTGGT AGCTGGCCTTCGAGAACAGTACATTAAGGCACAGGAGAATGCACAGGCTGAGCACAAATTCAGGGGACCC CCAGGGCCCACCCCGGCCATGGGGACAAACGCGAGCGTCAAGTCAAAGCTGCACAGACAGGCCCTGGTCC TGCAGGTGTCTACACAGGAACTGGAGGCAGAGATGGCTCTGAGAGCCCTAAAGGACGGAAAGGTGGCAGT AGCCCTGAGCAAATGCAGGGATTTACTTAAGCATTACTGCAATGCCGACACAGGGCGATTGCTGTTCGTG GTGTGTCAGAAGCTCTGTCACATGCTGGCTGACGACGTCCCAATGGTAGCCCCAGTAGGACTCAACCTTC CTTCTGAGATCCATGATCTCACATGCCACGCTGTCACCATCTGCAGTCCAGATTTCCTGCTGGATGTTCT AGAACTGAGTAAATACACTTTGACCGCTGTGGAGCTTTGCAGACAGTGCCAGATGGATGACTGTGGGATT CTGATGAAGGCTGCCGTCGGGACACACAAGGATCCCTATGAAGAGTGGTCCTACAGTGACTTCTTCAGTG AAGACGGAATTGTTCTTGAGTCACAGGTGGTGCTGCCTGTCATCTATGAACTGATCTCATCAGTGGTGCC CCTGGCAGAAGGCAAAAGACGCCCTTTGGATTCTATAAGCTTGCCATACTGTTCCACCAGTGAAGGAGAA AACCTTATTCTCCCCCTTGTCAGTTCCATCTCTGCTCTGCTCCGGAACCTTCAGGAATCCAGTCAGTGGG AACTGGCGCTCAGGTTCGTGGTGGCCTCCTTTGGTACCTGCCTGCAGCACTCTGTGTCTAACGTCATGAG CATGAGTTTAAGTGAGAAGCTACTTGGCAAGAGCACACTGGCGAGCTCAAGGCAGATCATTATGGAACTG AAAGAGAAGTCCATCGCATTCATCAGAGGAAGCGCCACGACACTCCTGCATAAAGTGTTTAACTGTCGTC TGGTGGATCTGGACCTCGCCTTGGCTTACTGCACTCTCTTACCGCAAAAGGATGTGTTTGAAAACCTCTG GAAGTTCATAGATAAAGCGTGGCAGAATTACGACAAAATCCTGGCATTATCTCTGGTAGGCTCTCGGCTG GCAAATCTCTACCAGGATGTTGAAACAGGACTTCGGTTCCATGAACTCAGTATCGATGCCAAGTGGGGAA TTCGCCTTGGTAAACTTGGTATTTCCTTTCAGCCGGCTTTCAGACAAAATTTCCTCAGCAAGAAAGACCT TATTAAAGCTCTCGTGAATAACATAGATATGGACACAAGCCTCATTCTGGAGTATTGCAGCACTTTTCAG CTGGACTCGGACGCCGCCCTTCGGCTGTTCATTGAGACCCTGCTCCGTAACACTACCAGCCAGAGCCAGG GAGGGGATGCCCCGGAGCCCACACAGCATCAGCATTCCAAACTCCTGCAGAAAGCCACAGAGATGGTCCC CTTGCTGAAGAGCACAAAGGACCTGGTGATCAGTCTTACCGAAATCCTTTATAAGGCTCTGAATCTTCTG AGACACTTGAAGTCATACAGAAGAATGTCCCCACCAGTGGACCTCGAGTACCAGTACATGCTGGAGCACT TGATAACGCTGCCACCAGCCGCCCAGACTAGACTGCCCTTTCACCTAATATTATTTGGCACAGCACAGAA CTTCTGGAAAATTCTCTCCTCGGAGCTCAGCGAAGAGTCACTTCCAACCCTGCTCTTAATCTCTAAGCTA ATGAAGTTCTCCCTAGATACTCTCTATGTGTCTACCGCCAAACACGTTTTTGAAAAAAACCTGAAGCCAA AGCTCCTGAAGTCAGCACAAGCCAGGTCCTCCGTGATGAGCAAGGAAGTGGATAAGCTCGTGCAGACCCT GGAGTCATACCTCCTGTCCATCGTCAACCCCGAGTGGGCCGTCGCCATTGCCATCAGTCTCACCCAGGAG ATCCCAGAAGGCCCCTTCAAGATGACTAGTTTGAAATTCTGCCTGTATTTAGCTGAGAGATGGCTACAAA ACATCCCACCTCAGGACGAGACATGCGAAAAGGTCAAGGCCTTGCAGAAGAAGCTGTACCTCCAGTTTCG GCTCTCGGGAACGGAGGCCGTGCTCATAGCCCACAAGCTGAATGATCAGGAATATTTAAGAGTGGTAGGG AAGCCAGCGCACCTCATCGTGAGCCTCTATGAGCATCCCAGCATAAGCGAAAGACTTTGTACCACATCTG GCAAAGATTATCCCGATATTCACGCAGCAGCTAAAGAGATAGCCGAAGTCAATGAGATTAACTTGGAAAA AATATGGGACATGCTGTTGGAGAAATGGTTGTGTCCCTCAACGGCGCCCGGTGAGAAAGCATCGGAGTTC TTTGAGCTTGAAGAGGATGAAGTGCTACATAGAGTCGTGTATCTCCTCCAGGCTCGCCCGATCGATTATA GCTCCAGGATGCTGTTCGTATTCGCCACTTCAGCCACAAGTACACTGGGCATACGGCAGCTAACGTTTGC CCACAGAGCACGAGCTCTTCAGTGCCTCCTCTACTTGGCTGACAAGGAAACCATAGAATCTCTCTTCAAA AAGCCCACCGAAGAAATGAAATCCTATCTGAAATGTATAACTTTCCTGGCATCATTCGAGACATTGAATA TTCCCATCACATATGAATTATTCTGCAACAGTCCCAAAGAAGGCATGATTAAGGGCTTGTGGAAGAACCA CAGCCACGAACCTATGGCAGTACGGTTGGTGGCAGAGCTTTGCTTAGAATACAAAATCTATGACCTGCAG CTTTGGAATGGGCTCCTGCAGAAGCTTCTGGGCTTCAACATGATCCCTTACCTAAGGAAAGTCCTGTCCT GCATTTCTAGCGTCCATTCCTTATGGCAGGTTCCCTACTTCAGCAAAGCTTGGCAGCGTGTAATCCAGAT CCCTCTGCTCTCAGCTTCCTGTCCTTTAAGGCCCAGTCAGTTAGCAGACTGTTGTGAGAGTCTCGTCGCC ATCCTTGAATGTCCAGTTTCAGATGACCTCGACATGATGGGCGTCGCCAAGCAGTATGTCCAGTTAGAGC TTCCTGCTTTTGCACTGACCTGTCTGACGCTCATGCCCCACTCTGAAAAAAGACATCAGCAGATCAAGAA TTTCCTGAATTCATGTGATGCTCAGATCATTTTACAGCAATTGGAGGAGCATATGAACGCTGGCCAGTTA GCAGGGCTTTCACAGCAGATTAGAAGTCTGGTTCTGAATCATGTCGTAAATAAGAAGGAATTTGGGATTT TGGCAAAGACAAAGTATTTTCAGTTGTTGAAACGGCACGTGATCAACACCGACAACGTCACTGAGTTGGT AAACTATTTAGCAAATGACTTGAGTTTGGATGAAGCATCATCTCTGATAACTGAGTATTCAAAGCATTGT GGGAAGCCTGTGCCAGCAGACACAGCTCCCTCTGAGATCCTGAAGACATTCCTTGGATCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107140
Insert Size	6153 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107140.1, NP_001100610.1
RefSeq Size	6479 bp
RefSeq ORF	6153 bp
Locus ID	304477

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