Npat (NM_001108147) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN202957 representing NM_001108147 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGTTACCCTCGGATGTAGCCAGGCTGGTATTGGGTTACTTACAACAAGAAAACCTCACATCTACTT GCCAGACTTTTATTTTGGAAAGTTCAAATTTAAAAGAATATGCAGAACACTGTACTGATGAAGGTTTTAT TCCAGCCTGCTTACTGTCCTTATTTGGAAAAAACTTGACAACAATTTTAAATGAGTATGTAGCTATGAAA GCAAAAGAAACATCAAATGATGTCCCAACAATAATGTCATCTCTGTGGAAGAAGCTGGACCACACGCTCT CTCAGATCAGGAGCATGCAGAGTTCTCCAGGGTTTGCTGCCCATCAGAGAGCTCGAACAAGAAATGGAAT TGCAGAAATCAAGCGGCAGCGATGGCTGGCATCTCAAGCAGCTCCCGTTAGCTCAGAGTTGCTGGTTTTA CCTTACGCTTCAGGACAGTTCACCACTTCTCCTTTGGCAGCCACACAAACTATCAAACCAACTGGACAGA TTTCAACTCCAGTGAGGTCAAATATTGTGGTGGTCAACCATTCACAGACACAAAATACTGTAACTAATAC AGCTGGAGAATCTTTGAATATCATTCCTGGTCCTCAGGAAAGGAAAACTCAAACCAGTTTAATGTCTCCT GGTAGACGTAAAAGTGAATCACAAAAGAAGAGTCTCCCGTCATCTGGAGCACATTCATCAAGGAATCTTC AGGATCCAAATGCATTTGCAGTAGAAAAACAAATGGTCATTGAAAATGCAAGAGAGAAAATATTAAGCAA CAAATCTCTTCAAGAAAAACTAGCAGAAAACATAAATAAGTTTTTGACTAGTGATAGTAATATTGCTCAA GTACCTAAACAGACAGAAAGCAGCCTGACAGAGCCAGAAACGTCAATTGATGAACTCCTAGGACTTCCGA GTGAAATCCACATGTCTGAAGAAGCAATACAAGATATACTGGAGCAGACAGAATCAGACCCAGCCTTTCA GGCACTCTTTGATCTATTTGACTATGGTAAAACAAAGAACAATAAAAACATATCACAAAGTGTCTCTAGT CAATCAGTGGAGACTAATCCTAATATAGTCATACCTGAAGAAACTAATCTAACAATTAAAAGTTCTTTTG AAACAGAAGAATCAGATGGGCAATCTGGTCAGCCTCCATTTTGTACATCCTATCAAAACGAAGATGTAGT GTTAAATGATCTGAAGAGTGGCAACAACCATGACGTTCTTCCACAGGAGTCCCAGGGAAACTTCTCCCAA ATAAGCGCTAACATTCAAAAAACAGCTGTACCTGCTGAACAGAAGTGTGCCCTTGACATTACCTTTGAGT CTGTGTCTAACTTAAGTGATTTTAACCAAAGAGGAAGCGCTACTGAATGTGATGAACATTGTTCTGAGTT ATACACCAGTCAGATGCCCACTGACGCTGGAGCGGCTGTGGGTGAAGAGAGTTCTCTGTCTCCAGATTCA CTGAACCAGTCTCAGTATCAGCCTGATCAGCCCCGCGTGCCAGTCACTTCATTTGTATCTCTTGGTGGTG AGACCAATGACAAAAACTTAATTCTCTCGGGGAAAAATTCCCAGCTTTTGTCCCAAAGTACTCCACTAAC TGGAAAGCCATCTAAAAGTCAATTTTGTGAAAATTCTAATAATATAATAAAAGTAAAAACTAATTCTCAT GCTTCTGAGTCACCAGATTCTAGTGACACTCACAGCAGTAAAGATGAAACTAACAGTAAATCACCAGTTG CTGCCCAGACACAACCAGATTGCCAAGATAATTCTCCACTGCAAAGCAGAACATCTCCTGTGATTATTGA AGGTTTAGGTGTAAATGTGACTGAAAAAATAGAAATTAGTATTGAAGAAACATCCCCTTCAGATAAACAG CTACCTAATGATTCAGCTTCTGTTGACTTAAATCCTATGGGAAGCGAGTCAGAGCCACTTCAGTCTGAGA ACACTCAAGAGCCTCCTTCTGTGAAAGATGGAGATACTATTTTTCTCTCTTTGAGTGGACATAACGGCTG TGAAGAAGTTGCACTGGTTCCTGGAGAAGGTAATCCTGTAAAAAATAATCATTCACTTTCTTCAGAATCT GGTGGTTCGGTGGGAGTCTCTGCTGAGACCCAAAATACTGATGGCAAAGCTAGCGACCCAGCAGAGGTAG ATGCGTCAAGCATTGTCTCTCTCAAAATCATCATTAGTGATGACCCATTTGTTTCCTCAGATGCAGAGCT TAACAGTGCTGTGTCTAGTATCAGTGGAGAAAACTTACCAACGATAATTCTGTCTTCTAAATCTCCTGCC AAAAATGCAGAATTCGTTACATGCCTATCTTCAGAAGAAACTGCAAGTGCTGTTGTATCTGTTGAAGTAG GTGATTCTGGCTCCATGGAGCAGAACCTTTTAGTGCTCAAACCTGAGGAACCCACGGTAAACAGCACTCA GAATGAAGATGGCATTGCTTTTTCAGCCAATGTTGCACCATGTGTTCCTAAGGACGGAGGATTCATACAA TTGATGCCTGCTACAAGCACGGCGTTTGGCAATTCAAGTAACATTCTGATAGCTACCTGTATGACTGACC CAACAGCCTTGGGACCAACTGTCAGTCAGTCTAATGTAGTAGTGTTGCCCGGGAGTTCTGCACCTTTGAC TGCACAGCCTCCTCCACAGCAGTTACAGACGCCACCTAAGTCTAACAGTGCATTTGCTGTCAGCCAAGCC GTGTCACCAAACTTTTCACAAGGATCTGCCATCATAATTGCTTCTCCAGTACAGCCTGTTCTTCAAGGAA TGGTGGGGATGATACCTGTGTCTGTAGTTGGACAGAATGGAAATACATTCTCCACTCCTCCTCAGCAGGT CCTTCATATGCCTTTAGCAGCACCTGTTTGTAATAGAAGTATCACTCAATTCCCCATTCCTCAAAAATCC CAGAAGGCTCAGGGACTCAGAAACAAGCCTATTACAGGAAAACAGGTAAATAATTTGACAAGCTTGTCCA GTCTTTCAGAAGCATGTCATACACAAAGAACTGAAGCTTCTGATAAGAATACAGCCACTGAACTTGGGAA AAAAATGGAAGACACTACAATTCCCCTATCAGCAGAGAGGGTAGCTCCTCCCAGCAGGCCATTTGAAAGT CATAGGCGGGTGCTTTGTTTTGATAGTACTGTTTCTTCTGTTGCAAATACACAGGGGCCACTATATAAAG TGACCTCCCAAAACAAAGAAAAGAAGGAAACATCTTTTCCTCGCCTTGACTCACCCATTCTGTCCTCTAC CTTAAAATCACCTACTAATAATGCCATCAAGAGAGAGAGGGAAAAAACTGTGCCCAAGATTTTATCTAAA TCAGAAACTGCCTCCAGTCGACACACCACTGTGAAAGAAGTCCAGTCTGAAAAGAAAGTTTCCCCCATAG AAACGACCCTTGAATCTTTCCATAAAGCAACAGCAAACAAGGAGAATGAACTGTGCAGTGATGGGGAAAG GCCGAAGAATCCAGACACCTCAAAACTGCCTGGCGGGCAGCAGAATGGGAGCTTGAAGAATGAAAAAGCC ATGGCCTCACTGCAGGAATTGACCAGAAAACAAGCCACACCCTCAAACAATAAAAATGCCACTACTGTAA GTGGGACTGTGAAGGATCAAAAACAAGAGCAGAGTAAACCTGCCGGCTCGTTAACTGGGCTAGAAACTCT GCAGGATGTTCCACTACACAACCCAGCAAATAGGACTGCTGATAGTGACTTGCCGGGACCCCGGACGACT GGCGCAGGAGCAGGAGAAAAGCATAAAGAAGAGCCTACTGACAGTATGAAGGCCCCTGCTAGTAGACGCT GTGGTGAGGAAGGCAGCGTGCCCAGAGCAATGATCCCAGCTGTCACTGCTGACTTGCCTGCCTGCAGCCC AGCCAGTGAAACGGGCAGCGAAAACAGCGTGAGCATGGCTGCCCACACGCTCATGATTCTCTCTAGAGCA GCAATTGCTAGGACTACAGCAACTCCTCTAAAAGATAACACGCAGCAATTTAGAACATCTTCAAGGAGCA CTACGAAGAAACGAAAAATTGAGGAATTAGATGAGTGTGAGAGGAACTCCCGCACTTCTAGTAAAAATCT TGCAAATTCATCAGTACCAATGAAAAAGAAGAAAATCAAGAAAAAGAAGCTACCTAGTTCATTTCCAGCA GGAATGGATGTGGACAAGTTCTTGCTATCACTGCATTATGATGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108147
Insert Size	4248 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108147.1, NP_001101617.1
RefSeq Size	6138 bp
RefSeq ORF	4248 bp
Locus ID	315666

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