Rab3gap2 (NM_001040154) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1311518
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203231 representing NM_001040154 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCTCCATCGTCCAGTTCTGCTCCTTTCAGGACCTGCAGTCCGCGCGCGACTTCCTATTCCCTC AGCTGCGGGAGGAGACCCCCGGAGCCCTGAGAAGGGACCCCAGTAAAACATCAAACTGGGAAGATGATAG TTGGGGAGCATGGGAAGAAACAGAGCCCCAAGAACCTGAAGAAGAAGGAAATACTTCTAAAACACAAAAA CATTCCTGGCTTCAAGAATGTGTTTTATCCCTCTCTCCAACCAGTGACCTTATGGTCATAGCCCGGGAGC AGAAGGCCGCATTTCTAGTGCCAAAATGGAAGCACAGTGATAAAGGGAAGGAGGAAATGCAGTTCGCTGT CGGTTGGAGCGGCTCCGTAAGTGCAGAGGAAGGGGAGTATGTAACCAGTGCTCTGTGCATCCCGCTGGCA AGTCAGAAGAGGAGTTCCACTGGGCGCCCTGACTGGACCTGCATTGTAGTGGGCTTTACGTCTGGTTATG TGCGGTTCTACACTGAGAGTGGAGTGCTTTTGCTTGCACAACTATTGAATGAGGACAGAGTGCTTCAGCT TAAATGTAGGACCTATGAAATCCCAAGACACCCTGGAGTGACTGAGCAGAATGAAGAGCTGAGCATCTTA TATCCGGCTGCCATTGTGACCATTGACGGATTTAGTCTTTTTCAGTCTCTTCGAGCTTGTCGAAACCAGG TAGCAAAAGCTGCAGCATCAGGCAATGAGAACATACAACCACCACCGTTAGCTTATAAGAAATGGGGTCT ACAAGATATTGACACCATTATCGATCATGCTAGTATTGGCATTATGACTCTGTCGCCGTTTGATCAAATG AAGACTGCTTCCAATATAGGTGGATTTAATGCAGCAATTAAAAACAGTCCGCCCGCCATGTCCCAGTACA TCACTGTGGGCTCCAGTCCGTTCACTGGCTTCTTCTATGCTCTAGAGGGAAGCACTCAGCCTCTGCTGTC TCACGTTGCACTTGCTGTTGCAAGTAAGCTGACCTCCGCCCTCTTCAGTGCCGCTAGTGGTTGGCTTGGT TGGAAAAGCAAGCACGAAGAAGAAACTGTCCAGAAGCAGAAGCCCAAGATGGAGCCAGCCACCCCGCTGG CTGTGAGATTTGGACTTCCGGATTCTAGACGCCACGGCGAAAGCATATGCCTGTCTCCTTGTAACACTCT GGCCGCAGTCACAGATGACTTTGGCAGAGTTATCTTGTTGGATGTAGCCAGAGGAATTGCAATACGGATG TGGAAAGGGTATCGGGATGCACAAATTGGATGGATTCAGATTGTAGAGGACCTCCATGAACGAGTACCAG AAAAGGGGGACTTCTCCCCCTTTGGAAATACTCAAGGGCCCAGTCGAGTAGCTCAGTTCCTTGTGATCTA TGCACCACGAAGGGGAATTCTGGAAGTGTGGAGCACACAGCAAGGACCTAGAGTGGGAGCTTTCAACGTG GGCAAACACTGCAGGCTGCTGTATCCTGGCTACAAAATCATGGGCTTAAACAATGTGACAAGTCAGAGTT GGCAACCTCAGACTTACCAGATCTGCCTTGTTGACCCTGTGTCTGCAAGTGTGAAGGTGGTGAACGTGCC TTTCCACTTAGCACTGAGTGACAAGAAGAGTGAAAGAGCCAAGGACCTGCACTTAGTGAAGAAGCTGGCC GCCCTGCTGAGAGCAAAGTCCCCTAGGCCGGATTCATTTGAAGCGGAAATAAAGGAGTTGATTCTTGACA TTAAGTATCCTGCAACCAAGAAACAAGCTCTGGAAAGCATTTTGGCCAGTGACCGTGTGTCATTTTCCTG CCTTAGAAATGTTACTCAGACTTCAATGGACACTTTAAAAAACCAAGAGCTTGAGTCTGTCGATGAAGGA CTGTTACAGTTTTGTGCCAGTAAATTGAAATTACTACATCTTTATGAGTCTGTCAGTCAGTTAAATACTC TTGACTTTCACTCAGATACACCGTTCTCTGATAATGATTTGGCTGTGTTGCTAAGGCTGGATGATAAAGA ACTCCTGAAGCTCCGGGCCTTATTAGAGAAATACAAACAAGAAAACACCAAGGCCACTGTGCGGTTCTCT GAGGATGCAGATGGAGTGCTGCCTGTTAAGACCTTCCTTGAATATCTAGACTATGAGAAGGATGCACTGA GCATAAGGAAGACCAGTGAAGAGGAATGTGTGGCCCTAGGCAGTTTCTTCTTTTGGAAGTGTTTGCATGG AGAAAGCTCCACTGAAGACATGTGCCACACTCTGGAGTCTGCTGGACTTAGCCCACAGCAGTTACTGTCT CTGCTCCTAAGTGTTTGGCTTTCGAAGGAAAAAGATATTTTGGACAAACCACAATCTGTCTGCTGTCTTC ATACCATGCTGTCCCTGCTGAGCAAGATGAAAGTGGCCATCGATGAGACATGGGATTCTCAGTCTGTGTC CCCGTGGTGGCAGCAGATGCGCATGGCCTGCATTCAGTCAGAGAACAACGGAGCTGCCCTGCTGTCTGCA CACGTTGGGCATTCTGTTGCTGCGCAGATGTCCAGCAGTGCCACAGATAAGAAATTTTCCCAGATGGTTT TGGATGCTGATGCAGAAGCCCTCACAGACTCCTGGGAAGCCCTTTCTTTGGACACCGAGTACTGGAAGCT CCTGCTGAGACAGCTGGAGGACTGCCTCATACTCCAGACTCTGCTGCATAGCAGAGCAAGCCCTCCAGCT GCCAAAGCGTCATCTCCACAGACAGAGCCACTTCCTAGGCTTTCTGTTAAGAAACTGTTAGAAGGGGGGA AAGGTGGTATTGCAGACAGCGTGGCCAAGTGGATATTTAAACAGGACCTCAGCCCTGAATTGTTGAAATG TGCCAACAGAGAAAAAGACGTAGAGAATCCAGACGAGCCCAGAGAAGGTATTGCCCGAAGTCCCCCTGAG GTGTCAGAGGTGGAGACAGACCTAGGAGCCGTACCAGACTTGCTCCGCCTAGCCTACGAGCAGTTCCCTT GTAGCCTTGAGCTTGACGTGCTGCATGCGCACTGCTGCTGGGAATATGTGGTTCAGTGGAACAAAGACCC AGAGGAAGCTCGCTTTTTGGTTAGATCCATAGAGCACCTGAGGCACATTCTTAATCCGCATGTTCAAAAT GGCATCTCGTTGATGATGTGGAACACATTCCTGGTGAAAAGGTTTTCTGCTGCTACCTACTTAATGGATA AGGTTGGGAAATCTCCAAAGGATAGGTTGTGTCGAAGGGACGTAGGGATGAGCGACACGGCACTGACCTC CTTCCTCGGCTCCTGCCTGGACCTTCTTCAGACGTCATTGGAGGCTGACATTAGCAGGGATGAAGTCCAG GTGCCAGTCCTGGACACTGAGGATGCCTGGCTGTCGGTGGAAGGCCCCACCTCTATAGTGGAACTGGCGC TGGAGCAGAAGCCTATCCACTACCCGCTGGTAGAGCATCATTCCATCCTGTGCTCCATCCTGTACGCCGC CATGAGCTTTTCTCTGAAGAGTGTGAAGCCTCTTGCCCTTTTTGACAGCAAGGGAAAGAATGCATTTTTT AAGGATTTAACTTCAATTCAGTTATTACCTAGTGGGGAAATGGATCCAAACTTTATTTCTGTAAGACAAC AGTTCTTACTGAAAGTTGTCAGTGCAGCCGTTCAGGCCCAGCACTCCAAGGACAGAGACCCCTCTGCACG AGCAGCAGACACTCACGGCCAAGACCTCAACTGGACAGCTCTGGCTGTGGACTTAGCCCACCACCTTCAA GTGAGCGAAGACGTCATTCGACGGCATTATGTGGGAGAGTTGTACAGCTATGGAGCTGACCTCTTAGGAG AGGAGGCCATTCTTCAAGTTCAGGACAAAGAGGTTCTGGCGTCTCAGCTGCTAGTGTTGACGGGACAAAG GCTGGCTCATGCACTTTTCCACACCCAGACGAAAGAAGGCATGGAGCTGCTGGCCAGACTCCCGCCCACA CTCTGCACCTGGCTGAAGGCAATGAACCCCCAGGATCTTCAGAACACTGGTGTACCTGTGGCAGCAACAG CCAAACTCGTGCATAAAGTGATGGAGCTCCTGCCAGAGAAGCACGGGCAGTACAGCCTCGCCTTACACCT CATCGAGGCTGTGGAAGCCATGGCTACCTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001040154
Insert Size	4164 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001040154.2, NP_001035244.2
RefSeq Size	4304 bp
RefSeq ORF	4164 bp
Locus ID	289350
UniProt ID	Q5U1Z0
Gene Summary	Regulatory subunit of a GTPase activating protein that has specificity for Rab3 subfamily (RAB3A, RAB3B, RAB3C and RAB3D). Rab3 proteins are involved in regulated exocytosis of neurotransmitters and hormones. Rab3 GTPase-activating complex specifically converts active Rab3-GTP to the inactive form Rab3-GDP. Required for normal eye and brain development. May participate in neurodevelopmental processes such as proliferation, migration and differentiation before synapse formation, and non-synaptic vesicular release of neurotransmitters.[UniProtKB/Swiss-Prot Function]

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