Col27a1 (NM_198747) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN203343 representing NM_198747 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTGGCGCGGGCGACCGCGGGGCTGGGGCCGTGCTGTCCGCCTGCTCCGGCGCTCCTGGGCGCAG GGCTGCGCTGGGGGGGATTTCTCTTCGCCTGGATCTTGGTCTCATTTTCCTGTCATCTGGCCTCCACCCA AGGAGCTCCTGAAGATGTGGATGTCCTCCAGCGGCTGGGCCTCAGCTGGACGAAAGCTGGGGGTGGCCGG AGCCCCGCACCCCCTGGTGTCATTCCTTTCCCATCTGGCTTCATCTTCACACAGCGGGCCAAGCTGCAGG CCCCTACTACCAATGTCCTTCCCACCACCCTGGGGCGGGAGCTGGCATTGGTGTTGAGCCTCTGTTCGCA CCGTGTGAACCATGCCTTCCTCTTCGCCATCCGCAGCAGGAAACACAGGCTGCAGCTGGGTCTGCAGTTC CTCCCCGGCAGGACTCTCGTCCACCTGGGACCTCGGCAATCTGTGGCCTTCGACTTGGATGTGCATGATG GGCGCTGGCACCACCTGGCCCTAGAGTTGCGTGGTCGCACGGTCACATTGGTGACAGCCTGTGGGCAGCA CAGGGTACCTGTTCCGCTGCCTTCCCGTAGGGACTCCATGCTTGACCCCCAGGGCTCCTTTCTCTTGGGG AAGATGAACCCCCGAGCTGTCCAGTTTGAAGGTGCACTCTGTCAGTTCAGCATCCATCCCGTGGCGCAGG TCGCTCACAATTACTGTGCCCACCTGAGAGAACGGTGCCGACAGGTGGACACGTATGGTCCCCAGGTGGG AGCCCTCTTTCCCTGGGACTCTGGCCCAGCCTTTGCTTTGCATCCTGAACCAGCCTTGCTTGGTCTGGGG AACCTGACCAGAAACCCAGCAACCCTAGGGTCCAGGCCTATAAGCAGGGGACTCATGGTGACTATGGCTC CTGCCGTGCCCACCAAGCCCCTAAGGATGGTCCACCAAGATGTCTCCAAACTCGGCTCTTCTCAGACCCC ATTGGTCCCTGCCAAACAGTCAGCCAGAAAAACACCTTCCCCCTTCCCTTCAGCTGCTCTGGCTAATTCC ACCAGGGTTTTTCACTCAGCTCCTGCCCAGCCACGACAAATCACAGCTACTAGCCCCACCAAACGTCCCC CGACCAAGCCTTCTGTTTCTTCCCTTTCAGTTACCCCCATGAAAAGTCCCCAGGCAATCCAGAAAGCAGG TACCCCTTCATTCTCAAGGCCCATTCCAACCACCCAGAAGCCAACACCCCTCACCTCCCACCCATCACCC TCCAAAGTCTCTAGTGCCACTGTGAGGCCTGTCCAGAAGACTTTCATGACACCCCAACCTCCAACCCTGA GTCCCCAAGCCCTGCACCCTATTACTGGCTTACCTAAGAAGTTCACTATCCCCACAGTAGCAAAACCTCA GTCCAAGATGACCAGTTGGGCCAGCAAGCCAGTCTTGGCCCGCACCAATGTCCCTAAGGCTCTTGAGCAA ACTGTTGTGGCCCAGTCCTCTGTTTCCTATCTGGGCTCTCAGACACTGGCTACAGCCCTCCCTCCTCTGG GTGTTGGAAATTCTAGAATGATGCCTTCCACACGTGACTCTACTTCAACTCCTGCTGGAAGCAAGAAAAT CACAGGATTGGAAGCTTCCAAGAAAACCAGACATAAGAGCAGCCCCCGGAAGCCCATACCTCTCAGCTCT GGGAAGACAGCCAGGGATGCTTCACCAAGAGACCTGACAACCAAGCCTAGCCAGCTGTCCACTCCAGCCC TGGTCCTTGCCCCAGCCCACCTCCTGTCCTCCAGCCCCCAGCCCACCAGCAGCAGCTTTTCTTTCTTCCA CCTGCCGGAACCTACACCTTTCCTGATGCTGATGGGGCCTCCTGGCTCCAAGGGAGACTGTGGCTTGCCG GGCCCTCCTGGGCTTCCAGGATTACCTGGATCTCCGGGTCCACGAGGTCCTCGGGGTCCTCCTGGGCCAT TTGGGAACCCAGGTCTACCTGGACCTCCTGGAGCCAAAGGACAGAAAGGGGACCCGGGACTCTCACCAGG ACAGGCTCACGATGGGGCAAAGGGCAACATGGGCTTGCCTGGGCTCGCCGGAAATCCAGGACCCATGGGA CGGAAGGGACACAAGGGCCACCCTGGAGCGGCAGGGCACCCTGGAGAACAGGGGCAGCCAGGACCTGAGG GCAGTCCAGGGGCCAAAGGTTACCCTGGCAGGCAGGGGTTCCCTGGACCTGTAGGAGACCCTGGCCCCAA AGGCAGCAGGGGTTACATTGGGCTTCCGGGGCTCTTTGGCCTGCCAGGGTCGGATGGAGAGCGGGGTCTC CCCGGCATTCCTGGCAAGAGGGGCGAGATGGGTAGGCCGGGCTTCCCTGGAGACTTTGGGGAAAGAGGTC CCCCTGGACTTGATGGGAACCCTGGGGAAATTGGCCTGCCAGGACCACCAGGAGTGCTCGGGCTCCTTGG TGACATGGGAGCATTAGGTCCAGTTGGCTACCCGGGACCAAAAGGCATGAAGGGACTGATGGGCGGCGTG GGGGAGCCCGGACTGAAAGGTGATAAGGGTGAACAAGGGGTGCCAGGTGTATCGGGAGATCCTGGCTTCC AAGGAGACAAGGGGAGTCATGGCTTGCCAGGGTTCCCAGGTGCCCGGGGGAAGCCAGGGCCTATGGGCAA AGCTGGGGACAAAGGCTCACTTGGGCTTCCTGGACCTCCTGGTCCTGAGGGTTTCCCAGGAGACATTGGC CCCCCAGGGGACAATGGCCCTGAAGGCATGAAGGGTAAGCCTGGAGCCCGAGGCCTACCTGGACCTCCTG GGCAGCTGGGGCCTGAGGGAGATGAAGGACCCATGGGGCCACCAGGAGTCCCAGGCTTGGAGGGTCAACC TGGAAGGAAGGGGTTCCCGGGGAGGCCTGGCCTGGACGGCTCGAAGGGGGAACCAGGGGATCCTGGACGG CCAGGGCCTGTGGGTGAACAGGGGCTTATGGGATTTGTTGGTCTGGTTGGAGAGCCTGGAATTGTTGGGG AGAAGGGAGACCGTGGAGTGATGGGACCCCCAGGGGCACCTGGACCCAAGGGGTCGATGGGCCATCCTGG AACACCTGGTGGTGTTGGGGACCCTGGAGAGCCTGGACCCTGGGGCCCTCCAGGATCTCGAGGACTGCCA GGCATGAGGGGAGCCAAGGGGCACCGGGGCCCTCGAGGACCTGATGGACCAGCTGGGGAGCAGGGGTCCA AGGGCCTGAAGGGTCGTGTAGGGCCTCGGGGCAGACCTGGCCAGCCAGGACAGCAGGGTGCAGCTGGCGA GCGAGGCCACTCAGGTGCAAAAGGCTTCCTTGGCATTCCTGGTCCCTCAGGCCCTCCAGGTGCCAAGGGC CTCCCAGGAGAACCGGGCTCTCAGGGACCTCAGGGGCCAGTTGGTCCTCCAGGAGAGATGGGACCCAAGG GGCCGCCTGGTGCAGTGGGAGAACCTGGCCTTCCTGGGGACTCCGGTATGAAGGGTGACCTTGGACCTCT GGGCCCTCCTGGGGAACAAGGGCTCATTGGGCAACGGGGAGAGCCAGGCCTCGAGGGAGACCTTGGCCCA GTGGGGCCAGATGGGCTGAAGGGGGACAGGGGTGACCCAGGTCCTGATGGTGAACATGGTGAAAAGGGTC AGGAAGGACTGAAAGGAGAGGAGGGGCTCCCTGGGCCCCCAGGCATCACTGGTGTCCGGGGTCGTGAAGG GAAGCCTGGCAGTCAGGGTGAGAAGGGCCAGAGGGGAGCTAAGGGTGCCAAGGGCTACCAAGGACAGCTG GGAGAGATGGGCATCCCTGGAGACCCTGGCCCCCCTGGCACCCCAGGTCCTAAAGGGTCCCGGGGCACCC TAGGACCAATGGGTGCTCCAGGACGGATGGGAGCCCAAGGAGAACCAGGATTGGCTGGTTATAATGGGCA TAAAGGCATCACGGGACCTCTCGGGCCTCCCGGGCCCAAAGGCGAGAAGGGGGAACAGGGCGAGGATGGC AAGACTGAGGGGGCCCCCGGGCCACCAGGAGAGCGGGGTCCTGTGGGTGATCGAGGAGACCGTGGGGAGC CAGGCGACCCTGGATACCCTGGTCAAGAGGGTGTTCAAGGCCTCCGTGGAGAACCAGGCCAGCAGGGCCA GCCCGGGCATCCAGGACCCCGGGGACGCCCAGGACCCAAAGGATCAAAAGGCGAAGAGGGCCCAAAGGGA AAGCCAGGCAAGGCGGGGGCATCAGGCCGGAGGGGGACCCAGGGGCTTCAAGGGCTGCCAGGGCCGAGAG GTGTAGTAGGGAGACAGGGCCCTGAGGGCATGGCTGGACAAGATGGGAATCCTGGCAGGGACGGTCGGCC AGGATATCAGGGAGAGCAGGGAAATGATGGGGACCCTGGCCCCGTGGGCCCTGCTGGGAGAAGAGGGAAT CCAGGTGTGGCTGGCTTGCCTGGAGCACAGGGGCCTCCAGGATTCAAGGGTGAAAGTGGGTTACCTGGGC AACTGGGTCCCCCTGGAAAACGAGGGACAGAAGGTGGAACGGGGCTTCCTGGGAACCAGGGGGAGCCAGG ATCCAAAGGCCAGCCGGGTGACTCTGGCGAGATGGGCTTCCCAGGAGTGGCTGGCCTCTTTGGACCCAAG GGTCCCCCTGGAGACATTGGCTTCAAAGGCATCCAAGGCCCTCGGGGTCCTCCTGGCTTGATGGGAAAGG AAGGTATCATTGGGCCCCCCGGAATGCTGGGACCTTCTGGACTCCCGGGTCCCAAAGGTGACAGAGGCAG CCGAGGGGACTGGGGACTGCAAGGCCCAAGGGGTCCTCCTGGTCCGAGGGGGCGGCCAGGTCCACCGGGC CCTCCTTGGCATCCTGTCCAGTTTCAACAAGATGACCTTGAGGCAGCTTTCCAAACGTGGATGGATGCTC ACGGAGCAGTCAGATTGGAGCAGGGGTACAGCTATCCGGACCAGCTGATGCTAGACCAGGGAGGGGAGAT CTTCAAAACCTTACACTACCTCAGCAACCTCATCCAGAGCATTAAGACGCCCTTGGGCACCAAGGAGAAC CCAGCCCGGGTCTGCCGGGACCTCATGGACTGCGAACAGAAGATGGCGGATGGTATCTACTGGGTGGACC CCAACCTCGGCTGCTCCTCTGATACCATCGAAGTCTCCTGCAACTTTACACATGGTGGGCAGACTTGCCT GAAGCCTATCACGGCCTCCAAGGCAGAGTTTGCTGTTAGTCGGGTCCAGATGAATTTCTTGCACCTGCTC AGCTCTGAGGGGACACAGCACATCACAATCCACTGCCTGAACATGACGGTGTGGCAGGAGGGACCTGCAC GCCCCTCTGCCAGGCAAGCTGTGCGCTTCCGTGCCTGGAACGGACAGGTCTTTGAAGCTGGGGGTCAATT CAGGCCAGAGGTGTCTATGGATGGCTGCAAGGTCCATGATGGCCGCTGGCATCAGACACTATTCACCTTC CGGACCCAGGACCCCCAGCAGCTGCCCATTGTCAGTGTGGACAACCTTCCACCTGTCTCGTCAGGGAAGC AGTACCGCCTGGAAGTTGGACCTGCATGCTTCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_198747
Insert Size	5568 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_198747.1, NP_942042.1
RefSeq Size	5568 bp
RefSeq ORF	5568 bp
Locus ID	298101
UniProt ID	Q80ZF0
Gene Summary	mouse homolog is a fibrillar collagen gene; may be a component of the extracellular matrices of cartilage [RGD, Feb 2006]

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