Ddx21 (NM_001037201) Rat Untagged Clone
CAT＃: RN204500
Ddx21 (untagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (Ddx21), (10 ug)
CNY 10070.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ddx21a; Ddx21b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN204500 representing NM_001037201
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGGAAAACTCCGCAGTGCGTCCAAGTCGGAGTCAGAAGGGACCGAGGAGAGTATGGAGACGCTGC AGAAGCCAAGCGAGAAGAAAACTAGAAAAGAGAAGCCAAAATCTAAGACTGACGAGGCAACAGAAGGAGT GGAAGAGGCCGCTTCCTCCAAAGTGAAAGCAGTTAAAAAGAAAGGGCCTTCTGAGGATGATGTGGGTCCC CCTAAATCCAAGAAGGCCAAGAAGCAGGAGGAGGAGCCTCAAGATGACCCTGCTTCTAAAAGCAAAACCT CCAAGAAGAAAAAGGAGCCCCTAGAGAAGAAAGCACCCTCTGCTAAAACCAAAGAAATGAAAGCCGAGGA GCCTTCTGAGGAAGAGGCAGACGCTCCTAAGCCCAAAAAGACAAAGAAAGGGAAGGAAGCAAACGGAGAT GTGGGAGAGAAGAGCCCCGGGCTGAAGAATGGGCTCTCCCATCCCAAGCCAGACTCCAGCTCCACCCAAG CTCCCGGAGAGGAAAGCGAGACAGAAAAGGAAATACCCGTGGAGCAGAAAGAAGGAGCCTTCTCTAATTT TCCCATATCAGAAGAGACTGTCAAACTTCTCAAAGCTCGTGGGGTGAACTTCCTCTTCCCTATACAAGCC AAGACATTCCACCATGTGTACAGTGGGAAGGACTTAATCGCCCAGGCACGCACAGGAACCGGGAAGACCT TCTCCTTTGCCATCCCTTTGATTGAGAAACTTCAAGGTGGGCTGCAAGAGAGGAAGAGAGGCCGAGCCCC TCAGGTGCTAGTCCTTGCACCTACAAGAGAATTAGCAAATCAAGTGAGCAAAGACTTCAGTGACATCACC AAAAAGCTCTCAGTAGCCTGTTTTTATGGTGGAACTCCCTATGGTGGTCAAATCGAACGAATGCGGAGTG GGATTGACATCCTGGTCGGGACCCCAGGTCGTATTAAAGACCACTTGCAGAATGGCAAGCTAGACCTCAC CAAACTTAAACATGTTGTCCTGGATGAAGTTGATCAGATGTTGGATATGGGCTTTGCTGATCAAGTGGAA GAAATTTTATGTGTGGCATACAAGAAAGATTCTGAAGACAACCCCCAAACATTGCTTTTCTCTGCAACTT GCCCTCATTGGGTATTTAATGTTGCTAAGAAATACATGAAATCTACATACGAACAGGTGGACCTGATTGG TAAAAAGACTCAGAAAGCAGCCATAACTGTGGAGCACCTGGCGATTAAGTGTCACTGGACAGAGAGGGCA GCGGTGATTGGGGATGTGATCCGAGTGTACAGTGGTCACCAAGGGCGTACAATCATCTTCTGCGAAACCA AGAAGGATGCACAGGAGCTGTCGCAGAACACGTGCATAAAGCAGGATGCCCAGTCCTTACATGGCGACAT TCCACAGAAGCAAAGGGAAATCACCCTGAAAGGTTTCCGAAATGGCAATTTTGGAGTTTTGGTGGCAACC AATGTTGCTGCCCGTGGGTTAGACATCCCTGAGGTTGACCTGGTTGTTCAGAGCTGTCCACCAAAGGATG TGGAGTCTTACATTCATCGTTCGGGGCGGACAGGCAGAGCTGGAAGAACAGGGGTTTGCATCTGCTTTTA TCAGCACAAAGAAGAGTACCAGTTAGCACAAGTGGAACAGAAAGCGGGAATTAAATTTAAACGGATAGGT GTTCCTTCTGCAACAGAAATAATAAAAGCCTCCAGCAAAGACGCAATCAGGCTCTTGGATTCTGTGCCTC CCACTGCTATTGGCCATTTCAAGCAGTCGGCTGAGAAGCTGATCGAGGAGAAGGGAGCTGTAGAGGCCCT GGCCGCAGCTCTGGCCCACATCTCGGGGGCCACATCGGTGGACCAGCGCTCCCTAATCAACTCACAAGCG GGCTTTGTGACCATGATCCTGCGGTGTTCTGTTGAGATGCCCAACATTAGTTATGCCTGGAAAGAACTTA AGGAGCAGCTGGGTGAGAGCATCGATGCCAAAGTGAAGGGGATGGTCTTCCTCAAAGGAAAACTGGGAGT TTGTTTTGATGTCCGCACTGAAGCAGTCACAGAAATAAAGGAGAAGTGGCACGATTCGAGACGCTGGCAG CTCACTGTGGCCACCGAGCAGCCGGAGCTGGAAGGACCCCCAGAAGGATACCGAGGCGGCAGGGGCCAGC GGGATGGCAGCCGTGGCTCTTTCAGAGGACAGCGGGGTGGAAGCAGGAACTTCCGGGGACAGGGACAGCG AGGAGGAAGTAGAAACTTCAGAGGACAGCGACCAGGAGGTGGCAACAAAAGTAACAGGTCCCCAAACAAA GGCCAGAAGCGGAGTTTTAGTAAAGCATTTGGTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001037201 |
| Insert Size | 2349 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001037201.1, NP_001032278.1 |
| RefSeq Size | 4413 bp |
| RefSeq ORF | 2349 bp |
| Locus ID | 317399 |
| UniProt ID | Q3B8Q1 |
| Gene Summary | RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes. Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77'. Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase). Involved in rRNA processing. May bind to specific miRNA hairpins (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR204500 | Ddx21 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (Ddx21), (10 ug) |
CNY 10070.00 |
|
| RR204500L3 | Lenti ORF clone of Ddx21 (Myc-DDK-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (Ddx21), (10 ug) |
CNY 11880.00 |
|
| RR204500L4 | Lenti ORF clone of Ddx21 (mGFP-tagged ORF) - Rat DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 (Ddx21), (10 ug) |
CNY 13020.00 |
