Ppip5k1 (NM_001080783) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Hisppd2a; RGD1311552
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN204552 representing NM_001080783 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGTCATTGACGGCCAATGAGGACGAGGACGAGAGCGCTACGGCCCACTTCTTTCTTGGAGCTGGAG ATGAGGGGTTGGGCACCTGTGGAATAGGCATGAGGACAGGAGAGAGTGACAGTGAACTCCTTGAGGACGA GGAGGATGAGGTGCCCCCAGAACCTCAAATTATTGTTGGCATCTGTGCCATGACCAAGAAATCCAAGTCC AAGCCAATGACCCAAATCCTAGAGCGGCTCTGCAGATTTGACTATCTGACCGTTGTCATCCTGGGAGAAG ATGTGATCCTAAATGAGCCCGTGGAGAACTGGCCACCCTGCCACTGTCTCATTTCTTTCCACTCCAAAGG CTTTCCTCTCGACAAAGCTGTTGCTTATTCCAAGCTTCGAAACCCCTTCCTTATCAATGATCTGACCATG CAGTATTACATCCAAGACAGGAGGGAGGTGTACCGGATCCTACAGGAGGAGGGTATTGATCTGCCACGAT ATGCTGTGCTCAACCGTGACCCTGCCTGTCCTGAGGAGTGCAACCTGATAGAAGGTGAAGACCAGGTGGA GGTTAATGGAGCTGTCTTTCCCAAGCCCTTCGTGGAGAAGCCAGTGAGTGCAGAGGACCACAATGTTTAC ATCTACTACCCTAGCTCTGCTGGAGGAGGAAGCCAGCGCCTCTTCCGTAAGATCGGCAGCCGGAGCAGTG TGTACTCTCCTGAGAGCAGTGTCCGGAAGACAGGATCGTACATCTATGAGGAGTTCATGCCAACGGATGG CACAGACGTTAAGGTGTACACAGTGGGGCCAGATTATGCCCATGCTGAAGCTAGAAAGTCTCCAGCTTTG GATGGGAAAGTTGAACGAGACAGTGAGGGGAAAGAGGTTCGATATCCAGTCATGTTGACTGCCATGGAAA AGCTGGTGGCCAGGAAGGTCTGCGTAGCTTTTAAGCAAACAGTTTGTGGATTTGACCTTCTTCGTGCCAA TGGTCACTCTTTTGTGTGTGATGTCAATGGCTTTAGCTTTGTCAAGAACTCAATGAAGTACTATGATGAC TGTGCCAAGATTCTGGGGAACACCATTATGCGGGAGCTTGCCCCACAGTTCCAGATACCTTGGTCCATCC CCACAGAAGCTGAGGACATTCCCATTGTCCCTACCACATCTGGCACTATGATGGAACTCCGTTGTGTCAT CGCAATCATCCGTCACGGGGACCGAACCCCAAAGCAGAAGATGAAGATGGAGGTGACGCACCCAAGGTTT TTTGCTCTATTTGAAAAACATGGTGGATATAAGACAGGAAAATTAAAACTTAAGCGGCCAGAGCAGCTCC AGGAGGTGCTGGACATTACGAGGCTGCTGTTGGCTGAGCTGGAGAAGGAACCAGGAGCTGAGATTGAGGA GAAGACTGGGAAGCTCGAGCAGCTGAAGTCTGTGCTGGAGATGTATGGTCACTTCTCAGGTATTAATCGG AAGGTGCAGTTGACCTACTACCCCCACGGAGTGAAAGCGTCGAGCGAGGGCCAAGATCTGCAGAGAGAGC CACCAGCGCCATCTCTCCTGCTGGTGCTCAAGTGGGGTGGAGAGCTGACACCTGATGGCCGCGTTCAGGC AGAGGAGCTGGGGCGGGCTTTCCGTTGCATGTATCCTGGAGGCCAGGGTGACTATGCTGGCTTCCCTGGC TGTGGGCTGCTTCGTCTCCATAGCACTTTCCGTCATGACCTCAAGATCTACGCATCTGACGAGGGCCGCG TCCAGATGACTGCTGCTGCGTTTGCAAAGGGCCTCTTAGCTCTAGAAGGGGAGCTTACACCCATTTTGGT ACAAATGGTGAAGAGCGCCAACATGAATGGGCTGCTGGACAGCGACAGTGACTCCCTGAGCAGCTGCCAG CACCGTGTGAAGGCACGACTGCATCACATCTTGCAGCAGGATGCACCCTTTGGCCCCGAGGACTATGACC AGCTGGCTCCGACTGGAAGTACTTCTCTGCTCAACTCCATGTCTGTAATCCAGAATCCTGTGAAAGTCTG TGATCAGGTATTTGCCCTGATTGAAAACCTTACCCACCAAATTCGGGAACGGATGCAGGACCCCAGCTCT GTAGACCTGCAGCTCTACCACAGTGAAACACTAGAGCTAATGCTACAGCGTTGGAGCAAACTAGAGCGTG ACTTTCGACAAAAGAGCGGGCGCTATGATATCAGTAAGATCCCCGACATCTATGACTGTGTCAAGTATGA TGTACAACACAATGGGAGTCTGGGACTTCAAGGCACAGCCGAGTTGCTACGTCTTTCAAAAGCTCTGGCC GATGTGGTCATTCCCCAGGAATACGGCATCAGTAGAGAAGAGAAAGTGGAAATCGCTGTGGGCTTCTGCC TTCCACTGCTGCGGAAAATCCTACTTGACCTGCAGAGAACTCACGAGGATGAGTCTGTCAACAAGCTGCA CCCCCTGTACTCGCGTGGAGTGCTCTCTCCAGGCCGCCATGTTCGAACTCGGCTCTATTTCACCAGTGAG AGCCATGTTCACTCCCTACTCAGTGTCTTCCGTTATGGGGGACTTCTCGATGAGACCAAGGATGCACAGT GGCAGCGAGCTTTGGCGTATCTTAGTGCCATCTCAGAGCTCAACTACATGACCCAGATTGTTATCATGCT TTATGAGGACAACACACGGGACCCCTTGTCAGAGGAGCGGTTCCATGTGGAGCTACATTTCAGCCCTGGT GTGAAAGGAGTTGAGGAAGGCAGTGCCCCAGCCGGCTGTGGATTCCGTCCAGCCTCTTCTGAGAACGAGG AGATGAAAACAGATCCAGGCAGCATAGAGAACCTATGCCCAGCAAAGCCGTCAGATGAGCCAGACCGAGC CCTGCAGACCTCACCCCAGCCTGTTGAGGGCACTGGCCTCCCGAGGAGATCACCCCTCATTCGTAATCGA AAAGCTGGCTCCATGGAGGTGCTGTCTGAAACTTCGTCTTCAAGACCTGGTGGCTATCGGCTGTTTTCAT CTTCACGGCCACCAACAGAGATGAAACAGAGCGGCCTAGGCTCACAGTGCACAGGGCTGTTCAGCACCAC AGTGCTGGGCGGCTCCTCCAGTGCTCCGAATCTTCAGGACTACGCCCGCACCCATGGCAAAAAGCTCCCG CCTGCCGGTCTAAAGCACCGAGATGAGCTCTTGTTTGTCCCGGCCGTAAAACGATTTTCTGTGTCGTTTG CAAAGCATCCGACTAACGGATTTGAAGGGTGCTCCATGGTACCTACCATCTACCCCCTGGAAACACTGCA CAATGCCCTTTCCTTGCGCCAAGTGAGTGAATTCTTGACTAAAGTCTGCCAGCGCCACACTGATGCCCAC GCACAGGCATCTGCAGCCCTCTTTGACTCTATGCACAACCACCAGGCCTCAGATAACCCGTTTTCCCCAC CCCGCACTCTTCATTCGCCACCCCTGCAACTTCGACATCGCTCTGAAAAGCCACCTTGGTACAGCAGTGG GCCTTCCAGCACTGTGTCCAGTGCTGGCCCTTCCTCTCCTACTACGGTGGATGGAAACTCACATTTTGGC TTCAGTGATCAGTCCTCTGTAAATACACAAATGATTGAAGAAAAGCAAGGCCTTGGGTTGCTCCAGGAGA CTCCTGGAGATGGAACACCAGAGTTCCACATAGAACTTGCTGAGTCAACCCAGTCCCCTCAAGAGCCACC TGTGGAAATCAGCCCTCCAGGAAGTCAGGATGATACTGAGGTCAACCAAACATGCCAGGAGGTCCCAGAC ACCATCCAGCCATGCCATGACATCCTTGAGGAGATTGGCCAGCCTAACCAGGAGGTCCCTGACATCAGCC AGCTTTTGCTGAAGAACCATGATACTGCTACCAACACGTGTCAACCATGCCAGGCCAGCCAGCTGTCTAA GAAAGTTTATGAGGAAATTTGTCAGCTTTGTCAAGACAATCCTGAGGAGAGTAACCAGCTATGCCAGGAA GTCTCAGTAGAGCTGGGCAGGATGGTCCACAGATTCCCTGTAAGCATTGGTAGCACCACCCAGGAAACCC TTATGGAAATTGGCAGGCCAACCCAAGAGATCCCCGAAGAACCATGCCAGGAGTTCTCTGAGAAGGTTGG CATGCTGACTCAGAAAGCCTCTGCCATCAGTGAGTTATCTCAAGACATCCTTGAGACTGACAATCCTTCC CAAGAGCTCTCTGAGGAGACTGATCTACAGGCTCAGGAAGTCTCTGAGGAGATTGATCAGGAGCCCGAGG TGGTCCCTGAGGTGGTTGATGAGCTGTCTAATGAAGATATCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001080783
Insert Size	4317 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080783.1, NP_001074252.1
RefSeq Size	5485 bp
RefSeq ORF	4317 bp
Locus ID	311355

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