Nup205 (NM_001108620) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205477 representing NM_001108620 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGTTGGCGGTAAATTCGGCTGCCAGCCTATGGGGACCCTACAAGGACATCTGGCAGACAG TCGGAAATGCTCTTTGGAGAAGACAGCCTGAAGCTGTCCACCTCCTTGACATGATTTTGAAGAAACACAA ACCCGACTTCATTTCACTATTTAAAAACCCACCAAAAAATGTTCAACAGCATGAAAAGATTCAGAAAGCC AGTTCGGAGGGTGTCGCCATTCAGGGGCAACAAGGAACCAGACTTCTCCCTGAGCAACTCATTAAAGAAG CATTTATCATCAGTGACCTTTTTGACATTGGGGAATTGTCAGCTGTTGAGCTTCTCCTTGCTGGAGAACA TCAGCAACCACATTTCCCTGGCCTCACCAGAGGATTGGTAGCTGTCCTTTTATACTGGGATGGAAAGCGG TGCATTGCAAACTCTCTCAAAACCCTAATACAGTCTAGACGAGGGAAGACATGGACATTAGAACTCAGTC CAGAGCTGGTTTCCATGACAACACGCTTTACAGATGAGCTGATGGAACAAGGATTGACTTACAAAGTCCT TACTTTGGTGTCACAGATTGATGTGAATAATGAATTTGAGAAACTACAGAGAGACAGAGGTTTGGGCAGT GAGAAACACCGCAAAGAGGTTTCTGATCTCATCAAGGAGTGCAGGCAGTCTCTGGCTGAAAGTCTTTTTG CTTGGGCTTGCCAGTCACCGTTAGCTAAAGATGACACCCTCCTGCTTATTGGACATTTAGAAAGAGTGAC AGTCGAGGCCAACGGCTCCCTGGATGCAGTAAATCTGGCTCTCCTCATGGCTCTTCTGTACTGTTTTGAT ACCAGCTTTATAGAACAAAGCACAGAGGAACGAGATGATATGATTCACCACCTTCCCCTGTTGACAGAGA GACAGTACATTGCCACAATTCACTCTCGCCTCCAGGACTCCCAGCCTTGGAAGCTACCTGGGCTGCAGGC CACTGTTAGACTTGCCTGGGCACTGGCACTGAGGGGGATATCCCAGCTACCTGATGTGACAGCCCTGGCT GAGTTCACGGAAGCAGATGAAGCGATTGCAGAACTCGCCATTGCCGATAATGTCTTCCTGTTCCTCAGCG AGTCTGTCGTGCTGGCTGAAAACTTCTATCAGGAAGAGTTTTATATTCGCAGAATCCATAATCTTATTAC AGACTTCCTTGCATTTATGCCAATGAAAGTGAAGCAGCTGAAGAATCGGGCAGATGAAGATGCTCGAATG ATTCACATGAGTATACAGATGGGTAACGAACCCCCCATCTCACTTAGAAGGGACCTGGAACACTTGATGC TCCTGATTGGTGAACTATATAAAAAAAACCCTTTTCATTTGGAGCTTGCTCTGGAGTACTGGTGTCCCTC AGAGCCTCTGCAGACTCCCACGGTCATGGGCTCTTACCTGGGAGTAGCTCATCAGCGCCCTCCTCAGCGC CAGGTTGTCTTGTCAAAGTTTGTTAGACAAATGGGTGATCTGTTGCCGCCAACTATTTACATTCCTTATT TGAAGATGCTCCAGGGCTTGGCCAATGGGCCTCAGTGTGCCCACTACTGTTTTAGCCTGCTCAAAGTCAA CGGTAGTAGTCATGTTGAAAACATTCAGGGAGCAGGTGGCAGTCCTGTTTCCTGGGAGCATTTCTTCCAC TCCTTATTGCTCTATCATGAGCATCTCCGAAAGGACCTGCCAAGTGCAGATAGTGTCCAGTACCGGCACC TTCCTTCACGCGGCATCACCCAGAAGGAGCAAGATGGGCTGATCGCTTTTCTCCAGCTGACATCTACTAT CATTACTTGGAGTGAAAATGCTCGCCTGGCGCTCTGTGAACATCCCCAGTGGACTCCCGTTGTGGTGATT TTGGGGCTCCTGCAGTGCAGTATTCCTCCAGCTCTCAAAGCTGAGCTACTGAAGACACTTGCAGCTTTTG GGAAGTCTCCTGAAATTGCCGCTTCTCTCTGGCAGTCATTGGAGTACACGCAGATACTGCAGACTGTCAG GGTTCCAAGCCAGAGGCAAGCTATTGGTATTGAGGTTGAACTCAATGAAATAGAGTCCCGGTGTGAAGAG TATCCCCTGACTAGGGCCTTTTGCCAGTTGATCAGTACCCTGGTGGAGAGCTCATTCCCTTCTAATCTGG GTGCTGGCCTGCGCCCCCCTGGCTTTGACCCTTATTTGCAGTTCCTGAGAGATTCTGTGTTTCTCCGATT CCGTACAAGAGCTTACCGGAGAGCCGCTGAAAAGTGGGAAGTCGCTGAGGTTGTCCTGGAAGTGTTTTAT AAGCTGTTGAGAGATTATGAGCCCCAGCTTGAAGATTTTGTAGATCAGTTTGTGGAGCTACAAGGAGAAG AGATCATAGCCTATAAGCCACCTGGGTTTAGTCTGATGTACCATCTTCTGAACGAGTCACCGATGTTGGA ACTTGCCCTCAGCTTACTGGAAGAAGGAGTTAAGCAGCTTGATACCTATGCTCCTTTCCCTGGAAAGAAA CACCTGGAAAAAGCAGTTCAGCATTGCCTCGCACTCCTCAACCTCACTCTGCAAAAGGAGAACCTTTTCA TGGACCTTCTGAGAGAGAGTCAGCTGGCTCTCATCGTCTCGCCTCTGGAACAGCTCTTGCAGGGAATTAA CCCTAGGACTAAGAAGGCAGACAATGTGGTTAACATCGCCAGGTATCTGTATCATGGAAACAGTAATCCT GAATTGGCATTTGAAAGTGCAAAGATCCTCTGCTGTATCTCTTGCAATTCCAACATTCAGATCAAAATGG TTGGCGATTTCACACATGACCAGAATGTGAGCCAGAAGCTGATGGCTGGATTTGTAGAGTGCTTGGATAG TGAAGACACAGAAGAATTTGTACGTGTAGAAGAGGGCTCAGAATTAGAAAAGAAATTAGCTGCAATCCGT CACGAAACGAGAATCCACATCTTGAATCTTCTCATCACATCTCTAGAACGCAGTCCACCCAATCTCGCCC TCTACCTGTTGGGCTTTGAATTGAAGAAACCCATCAGTACCACGAATCTGCAAGACCCAGGAGTACTAGG CTGCCCTCGGACGTGCCTTCATGCCATCCTGAACATTCTGGAGAAAGGCACCGACGGGAGGAGCGGCCCT GTGGCCGTGCAGGCGTGTCCACAGCTGGCCGAGCTGTGCTACCAGGTGATATATCAGTTATGTGCATGCT CAGATACATCTGGTCCTACTATGAGGTACTTGAGAACCAGCCAGGATTTCTTATTTTCTCAGTTGCAACA TCTACCTTTCTCGAACAAAGAGTATGAAATCTCTATGCTGAATCAAATGTCATGGCTTATGAAGACTGCC TCAATAGAGCTAAGAGTGACGTCTCTGAATCGCCAGCGCTCACACACACAGAGGCTTCTGCATCTCTTAC TGGATGACCTGCCGGTGAAGCCGTACTCTGATGGTGAAGGAGGAATGGAAGATGAAAATAGATCTGTCTC TGGGTTCCTTCATTTTGACACTGCCACAAAAGTGCGTCGAAAAATTCTAAGTATTCTTGATTCGATTGAC TTCAGTCAGGAGATTCCTGAGCCTTTGCAGCTGGATTTTTTTGACCGTGCCCAGATTGAGCAAGTCATTG CCAATTGTGAGCACAAGAATTTACAGGGGCAGACGGTCTGCAATGTCAAGCTTCTTCATAGGGTTCTCGT AGCTGAAGTCAATGCTCTTCAGGGTATGGCAGCAATAGGCCAGAGGCCGCTGCTGATGGAGGAAATCAGC ACTATTCTTCAGTATGTCGTAGGAAGAAACAAGTTGCTGCAGTGTCTTCATGCAAAGCGGCATGCACTGG AATCCTGGAGGCAGCTGGTAGAAATTATCCTGACAGCTTGTCCTCAGGAGCTCATTCAGGCAGAAGATCG GCAGCTGATTATTCGTGACATTTTACAAGATGTGCATGATAAGGTGCTGGATGATGAAGCCGCACAGGAG CTAATGCCAGTGGTTGCCGGTGCTGTGTTCACCCTGACTGCTCACCTAAGCCAGGCTGTGCGCACGGAAC AGAAGCAGCCATTGGTCTCAGGACCTGCAGAGGCCCATTATGCTTTCATGCTTGATAGTTCTTTCACCTC AGCTCCTGCAGCAGAGAGCCAGCCGGTGGGCTTTGCTTCTATTGGAGATTCTTCACTTCATATCATACTG AAGAAACTGTTGGACTTCATTTTGAAGACAGGTGGTGGCTTCCAGCGTGTAAGGACTCACCTGTATGGCT CGCTGCTTTATTACTTACAGATTGCCCAGAGACCAGATGAGCCGGACACATTAGAAGCTGCTAAGAAAAC TATGTGGGAAAGGCTGACTGCCCCTGAAGATGTGTTTAGTAAATTACAGCGAGAAAACATGGCTATCATT GAGAGTTACGGCGCTGCGCTCATGGAGGTGGTCTGTCGAGATGCTTGCGACGGTCATGAGATTGGAAGGA TGCTGGCCCTGGCTCTCCTTGATCGGATTGTCTCTGTGGACAAGCAGCAGCAGTGGCTCTTGTACCTTTC TAACAGTGGCTATTTGAAGGTCCTTGTAGACAGCTTGCTGGAAGATGATCGCACTCTACAGAGCTTGCTG ACTCCACAGCCTCCTCTCTTAAAAGCCCTTTATACCTATGAGTCCAAGATGGCATTTCTCACAAGAGTGG CTAAGCTACAGCAAGGTTCATTAGAACTCCTAAGATCAGGGGTGATTGTGAGGCTGGCACAGTGCCAAGT CTATGATATGCGCCCAGAAGTGGACTCTCATAGCATGTTTGGCATGAGAGACCCACCAATATTCATCCCA ACGCCTGTGGACCGATACCGGCAGATTCTCCTTCCAGCCCTCCAGCTATGCCAGGTCATCCTCACATCCA GTATGTCCCAGCACTTGCAGGCAGCAGGGCAGGTGCTGCAGTTTCTCGTTTCACATTCTGATACCATCCA AGCGATTCTGCGCTGCCAGGATGTTAGTGCTGGCTCTTTGCAGGAATTAGCCCTGCTGACAGGAATCATA AGTAAAGCAGCGCTTCCTGGTATGTTAAATGAACTTGATGTAGATGTAAATGAAGGATCCTTAATGGAGC TCCAGGGACACATCGCAAGGTTTCAGCGCCAGTGCCTAGGCCTACTGAGTCGCTTCGGTGGCTCTGACAG ACTGCGGCAGTTTAAACTCCAAGAAGATAATGTGGAGGGAGACAGGTTGAGCAAGAAAGATGAAATTGAG CTGGCCATGCAGCAGATTTGTGCCAATGTAATGGAATATTGCCATTCGTTCATGCAGCAGAACTCCCCTA ACCTCCAGAATGCTGTGTGTCTCTTCACCCCTAGCCTTTCCGAGACGGTTAACAGAGATGGAGCACGGCA AGATACCCAGGTTCCAGTCGTTCCATACTGGCGCCTGCCTGGCTTGGGCATTATTATCTACCTGCTGAAA CAGAGTGCCAGCGATTTCTTTAGCTATTATGACAGTCACCGTCGCAGTGTCAACAAGCTGCAGAATGTAG AACAACTTCCTCCAGATGAGATAAAAGAGTTATGCCAGTCTGTGATGCCTGCTGGCATTGATAAAATCTC TACGGCGCAGAAGTGTGTCTTAGCAAGACGGCGTCTGGTGAAGCTGATCAACAACCGAGCCAAACTGCTG TCCCTCTGCTCTTATATCATCGAGACCTGCCTGTTTATTCTTTGGCGCCATCTGGAGTATTACTTGTTAC ATTGCACTCCAATGGATTCCCAAGATTCACTGTTTGCCTCTAGGACTTTACTTAAAAGCAGAAGACTACA GGAGCCCAATCTGGACTTCAGCAGAAGTGGACTGGCTCAAGTGAACCAGCACGATATAGACCAGCTGCAG TCTGATGCCATCAACGCTTTTGGAGAATCACTGCAAAAGAAACTGCTTGACATCGAAGGGTTGTATTCAA AAGTTCGATCCCGCTATAGCTTCATACAGGCTCTTGTCAGACGTATCCGTGGTCTGCTGAGGATATCAAG GAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001108620
Insert Size	6027 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001108620.1, NP_001102090.1
RefSeq Size	6230 bp
RefSeq ORF	6027 bp
Locus ID	362335

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