Impact (NM_001012235) Rat Untagged Clone
CAT＃: RN207359
Impact (untagged ORF) - Rat imprinted and ancient (Impact), (10 ug)
CNY 3990.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207359 representing NM_001012235
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGTGGAATCAGGGAGCAACCAGAGGCAGAATGAAGAAATCGAGGCAATGGCAGCCATTTATG GCGAGGAGTGGTGTGTCATTGATGAGGTTGCCAAAATATTTTGTATTAGAATCACTGACGACATGGATGA CCCCAAATGGACACTTTGTTTACAGGTGATGTTACCAAGTGAGTACCCAGGTACAGCGCCACCTGTTTAT CAGCTGAATGCTCCTTGGCTGAAAGGGCAAGAACGCGCAGAATTAGCCAAGAGCCTTGAGGAGATCTATA TGAAGAACATTGGTGAAAGTATTCTTTACCAGTGGGTGGAGAAAATAAGAGATGCCCTGATACAGAAATC TCAGATCACAGAGCCAGGCCCAGATGAGAAGAAGAAAACTGAAGAGGAAGAAGTTGAGGGTGAAGATGAC CCCATTCTAGAACACCCACCAGAAAATCCAGTTAAAACATGGGACCTCAAAATCAGTGAAAGTGCACCAG AAGCGGAAGAATTGCCTCCAATTGCTCATGGTGCTCCTATCACAGACCGAAGGAGCACTTTCCAGGCACA TTTGGCCCCTGTCGTCTGCATTGACCAGGTGAAGAAGGTTCTTGCCAAGTTGTATGAGAATAAGAAGATC GCCAGTGCCACCCACAACATCTACGCCTACAGGATATACTGTGAGGATAAGCAGACCTTCTTGCAGGACT CCGAAGATGATGGAGAAACAGCCGCGGGCGGCCGCCTTCTTCACCTTATGGAGATTTTGAATGTGAAGAA TGTCATGGTGGTGGTATCCCGCTGGTATGGAGGGATCCTGTTGGGACCTGATCGTTTCAAACACATCAAC AACTGTGCCAGGAACATCCTGGTGGAGAAGAACTTCACAAACTCCCCTGAGGAGTCAGCCAAGAGCTTTG GAAAGAAGAAAGTGAAAAAGGACAAGAAGAAGGGTGACCATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001012235 |
| Insert Size | 954 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001012235.1, NP_001012235.1 |
| RefSeq Size | 3386 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 497198 |
| UniProt ID | Q5GFD9 |
| Gene Summary | Translational regulator that ensures constant high levels of translation upon a variety of stress conditions, such as amino acid starvation, UV-C irradiation, proteasome inhibitor treatment and glucose deprivation. Plays a role as a negative regulator of the EIF2AK4/GCN2 kinase activity; impairs GCN1-mediated EIF2AK4/GCN2 activation, and hence EIF2AK4/GCN2-mediated eIF-2-alpha phosphorylation and subsequent down-regulation of protein synthesis. May be required to regulate translation in specific neuronal cells under amino acid starvation conditions by preventing GCN2 activation and therefore ATF4 synthesis. Through its inhibitory action on EIF2AK4/GCN2, plays a role in differentiation of neuronal cells by stimulating neurite outgrowth.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207359 | Impact (Myc-DDK-tagged ORF) - Rat imprinted and ancient (Impact), (10 ug) |
CNY 3990.00 |
|
| RR207359L3 | Lenti ORF clone of Impact (Myc-DDK-tagged ORF) - Rat imprinted and ancient (Impact), (10 ug) |
CNY 6080.00 |
|
| RR207359L4 | Lenti ORF clone of Impact (mGFP-tagged ORF) - Rat imprinted and ancient (Impact), (10 ug) |
CNY 6650.00 |
