Zbtb38 (NM_001012471) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1310136
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207423 representing NM_001012471 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGTCATGTCCCTCTCCAGGGACCTCAAGGACGACTTTCACAGTGACACGGTGCTCTCCATCCTAA ATGAGCAGCGCATTCGGGGCATCTTATGTGATGTCACCATCATTGTGGAGGACACCAAGTTTAAAGCCCA CAGCAATGTCCTGGCCGCCTCAAGCCTTTATTTCAAAAACATCTTTTGGAGCCATACGATCTGTATTTCC AGTCATGTCCTAGAGCTGGATGATCTGAAAGCCGAAGTGTTTACAGAGATTCTTAATTACATCTACAGCT CCACCGTGGTGGTCAGGAGACAGGAAACCGTCACTGACCTTGCAGCTGCAGGGAAAAGGCTGGGAATATC ATTCTTAGAGGACCTTAGTGACCGCAACTTCTCAAATTCCCCAGGTCCTTACGTAGTTTGCATCACTGAA AAGGGCGTGGTTAAAGAAGAAAAAAATGAAAAAAGGCACGAAGAGCCAGCTGTCACTAATGGGCCCAGGA TCACAAACGCATTTTCCATCATTGAGACGGAAAATAGCAATAGCATGTTTTCTCCCCTGGACTTGAGGGC AAGCTTCAAAAAAGTGTCAGATGCCATGAGAACCACTAGCCTTTGCCAGGAGAGAGCCAGTGTCTGCCCC GAGGCAGAGCCTGTCCGCACGCTTGCCGAACACTCATATGCCGTTTCTTCCATCACTGAGGCCTACAGGA GTCAGCCTCTGAGGGAACATGACAGCAGCAGTTCATCTGGTAAAACGGGAAAAGAAAATGGCGAAGCTCT CACCACAAAAGCAAAGCCATGCCGAAAGCCAAAGACCCAAACCCAGGATTCTGACTCAACCACAGAGAAC ATGCCACTCCCTCTAGTAACCTGCCCAGAGGTGAATCAAGAAAGAAGCCCACAGCCAGCTCCAATTCTGT CACACTCAGAACCTCCCAGCAGTGAAGGAGATGTCCATTTTCCCAGAGAAGATGAAAACCAGCCCTCTGA GACTCCTGGTCCCCCAGCAGCAGAGGTCCCGCCCCTCGTTTATAATTGTAGCTGCTGCTCCAAATCCTTC GACAGCAGCACACTGCTCGGGGCCCACATGCAGCTGCACAAGCCGACGCAGGACCCCTTTGTGTGCAAGT ACTGCAACAAGCAGTTCACCACCCTCAACAGGCTGGACCGGCACGAGCAGATCTGCATGAGGTCGAGCCA CATGCCCATGCCAGGAGGAAACCAACCCTTCTTAGAAAACTACCCCACCATTGGACAAGATGGAGGTTCA TTCACAAGCCCAGATTCATTGGTGCCAGAAAGTAGGATTGGTGAACTATCCAGCGCTGGGAGTGCCTTGT CAGACGCGGACCACATGGTGAAATTTGTGAATGGGCAGATGCTTTACAGCTGCGTTGTGTGCAAACGTAG CTATGTGACTTTGTCCAGCCTCCGGAGGCACGCAAATGTCCACTCGTGGAGAAGAACGTACCCTTGTCAT TACTGCAACAAGGTCTTCGCACTGGCTGAGTACAGGACACGACATGAGATCTGGCACACTGGAGAGAGGC GGTACCAGTGCATTTTCTGTCTTGAAACGTTTATGACCTACTACATACTGAAAAACCATCAGAAGTCTTT TCATGCCATAGATCACAGACTCTCCATCAGTAAAAAAACAGCAAATGGAGGCTTGAAGCCCACCGTCTAC CCATACAAACTTTACAGGCTCTTGCCTATGAGATGCAAGCGGGCACCTTATAAGAGCTACCGGAATTCTT CCTATGAAAGTGCTCAAGGAAACCGTCAAAGGAATGAATCCCCACCTGACACCTTCGTCATTCCGAATCT GCCAAGCTCTGAAATGCCCACACTGAACTTCCAAGATGGCAGAAACTCGTTGACCAGCAGCCCTGCCATC CCAGTGGAAACACCTTCATGGCAGGGTACACCCACTTCTGCCAAAGTAAAAAATGCAGAAGGCCTCAAAT GGAGGAAGCAGGCACTGAAAACCGATCTCGTCGATTCGGCTGAGGTGTCGATTTCTTCCATTGGAAACTC TGTCAGCACCACCCTGCAGGCAGAGCCCGTGTGTGTTTCGAGTGGGGAGCACTCGGCCTCCGTGATCAGC TACAGCGGCTTGGTGCCCTCTGTCATTGTGCACAGCAGCCAGTTCTCGTCGGTGATCAAGCACAGCAATG CCATTGCCTGCCTGGCCAACAGCAACCACCAGTCCCCTTCACAGCCTGTGGCGAGTCCGTCCCTGATCAA GGATAGCAAGCCTGAGGCAGATAAAGCCAGTAAACTCGCAAGCAGACCCAAAAACTCTAAGGAGAAAAAG AAAACCGTTCCATGTAACAGGGGAGAAATAACAGAGGAGGCAAAATATGTTGCTGATCTCGGAGGATCTT CGGGCAAAACCACAAATGTCGTCGAAGAAACCAGTAAGATTGAAACTTATATAGCAAAACCCGCTCTGCC CGGCACCTCCACAAATAGCAATGTTGCACCCCTTTGCCAAATAACAGTGAAAATTGGGAACGAAGCCATC GTGAAAAGGCATATCCTTGGGTCTAAGCTGTTTTACAAAAGAGGGAGAAAACCCAAGTATCAAATGCAGG AAGAGACATTGCCTCGGGAGAGTGACCCCGAAACCCGTGGAGACAGCCCTCTTGGGCTCTGCCAAGCTGA CTGTGTGGAGATGAGTGAAGCATTTGATGAAGTGAGTGACCAGGACTCCACCGATAAGCCGTGGCGCCCA TACTACAACTACAAGCCCAAGAAGAAATCCAAACAGTTAAGAAAAATAAGGAAAGTCAAGTGGAGGAAGG AACGAGGGAGCAGAAGCCCCGTGGGTCGGCGCAGGTATCCAGCTGAGCTGGATCGCGCAGAGATGGGTCG GCGCAGGTACCCAGCTGAACTAGATCGCTGCGCAGAGGTGAAATTGCCCCCAGATAAGGCCTCTGAAGAA GAGGAGAATAAAGAGATGCCCAAGCTGCAGTGCGAGCTCTGTGACGGTGACAAAGCAGCAGGGGCGGGAG CCGAAGGCAAGCCCCACCAGCATCTCACTCTGAAGCCTTACATCTGCGAGCTCTGTGCGAAGCAGTTCCA GAGCTCCTCCACGCTCAAGATGCACATGAGATGCCACACTGGCGAGAAGCCCTACCAGTGCAAGACCTGT GGGCGGCGCTTCTCAGTGCAGGGCAACCTACAGAAGCACGAGCGCATTCACCTGGGCGTGAAGGAGTTCA TCTGTCAGTATTGTAACAAGGCTTTCACGCTGAACGAGACGCTCAAGATCCACGAAAGAATCCACACAGG GGAGAAGCGTTACCACTGTCAGTTCTGCTTTCAGGGTTTCTTATATCTCTCCACAAAAAGGAACCACGAG CGGAGGCATGTCCGGGAGCATGACGGGAAAGGCTTTGCTTGCTTCCAGTGCCCCAAAATATGCAAAACAG CTGCTGCCCTCAGAATGCACCAAAAGAAGCACTTATTCAAGACCTTGACTAAGCAAGAGGAAACAGATGA CCTTTGCCACGAAAACTCCGATCTCTTGGAGAGTCAACCTTGCACTGATTCGGAAGACAGCGACCAAAAG GATGACATAAAAAAACCTTTGCTGAAAATGTCCTTTGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001012471
Insert Size	3612 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001012471.1, NP_001012489.1
RefSeq Size	3901 bp
RefSeq ORF	3612 bp
Locus ID	315936
UniProt ID	Q5EXX3
Gene Summary	Transcriptional regulator with bimodal DNA-binding specificity. Binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' but can also bind to E-box elements (5'-CACGTG-3'). Can also bind specifically to a single methyl-CpG pair. Represses transcription in a methyl-CpG-dependent manner. Plays an important role in regulating DNA-replication and common fragile sites (CFS) stability in a RBBP6- and MCM10-dependent manner; represses expression of MCM10 which plays an important role in DNA-replication (By similarity). Acts as a transcriptional activator. May be involved in the differentiation and/or survival of late postmitotic neurons (PubMed:15713629).[UniProtKB/Swiss-Prot Function]

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