Eif5b (NM_001110141) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207603 representing NM_001110141 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAAGAAACAGAAAAACAAGAGCGAAGACAGTACCAAGGATGATACTGACTTGGGCGCCTTGGCTG CGGAAATAGAAGGAGCTGGTGCTGCCAAGGAGCAGGAGCCTCAGAAGGGAAAGGGGAAGAAGAAAAAGGA GAAAAAGAAGCAAGACTTTGATGAGAACGACATTCTGAGAGAGCTGGAGGAGTTGTCTCTGGAAGCCCAA GGCATCGGAGCTGACAGAGATGCCGCTACAGTCAAGCCTACAGAAAACAATGAAGAAGAATCTGCCTCAA AACAAGATAAAAAGAAGAAAGGACAAAAAGGCAAGAAAACAAGTTTTGATGAGAATGATAGTGAAGAATT GGAAGATAAAGATTCAAAATCAAAAAAGCCTGCCAGACCTAACTCAGAGGTGCTCCTCTCTGGGAGTGAG GATGCAGATGATCCTAATAAACTTTCTAAAAAAGGTAAAAAAGCCCAGAAATCAACTAAAAAACGGGATG GGTCGGAAGAGGATGAAGACAATAGCAAAAGAAGTAAAGAACGTTCGAGAGTAAACTCCTCAGGTGAAAG TGGTGGTGAGTCTGATGAATTTTTGCAGTCCAGAAAAGGACAGAAAAAAAATCAGAAGAACAAGTCAGTT CCTACCATAGACAGTGGGAATGAGGACGATGACTCTTCCTTCAAAATTAAGACGGTGGCCCAGAAGAAGG CAGAAAAGAAAGAGCGTGAGAGGAAAAAGAGAGAAGAGGAGAAAGCAAAGTTGCGAAAGGTGAAAGAGAA AGAAGAGCTAGAGAAGGGCAGGAAGGAGCAGAGTAAGCAGAGGGAGCCTCAGAAGAGACCGGACGAGGAG GTGTTGGTGCTCAGAGGGACTCCTGATGCAGGTGCTGCCTCAGAAGAGAAAGGAGACATTGCTGCAACTC TGGAAGATGACAATGAAGGAGACAAAAAGAAAAAAGATAAGAAGAAAAAGAAAACAGAAAAAGATGATAA AGAAAAAGAGAAGAAGAAAGGCCCAAGCAAATCCACTGTTAAAGCTATCCAGGAAGCTTTGGCTAAGCTC AGAGAGGAGGAGGAGAGACAGAAGAGAGAGGAGGAGGAAAGGATTAAGCGGCTTGAAGAACTAGAAGCCA AACGAAAAGAAGAGGAACGATTAGAACAAGAAAAAAGAGAAAGGAAAAAGCAAAAAGAAAAAGAAAGAAA AGAACGGTTAAAAAAAGAAGGAAAACTGTTAACTAAGTCCCAGAGAGAAGCCAGAGCCAGAGCTGAAGTG ACCCTGAGACACCTCCAAGCGCAGGGTGTTGAAGTGCCATCAAAAGACTCGTTGCCAAAGAAGAGGCCAG TGTATGAAGATAAGAAGAAGAAGAAAACACCACAGCAGCTGGAGAGTAAAGAAGCACTTGAGACTGTGGA AGTCAGTGCCCCTGTGGAAGTTGTGGACCAAGGAGTACCAGAAAAGGAGGAGACACCGCCTTCTGTTGAC GCAGAGGAAGACGAAGAAACTGAAGATGCTGGGCTCGATGACTGGGAAGCTATGGCCAGTGATGAGGAGA GAGAAAAAGAAGGAAATATGATTCACATAGAAGTAGAAGAAAACCCTGAGGAAGAGGAGGAGGAAGAAGA GGATGAAGATGAAGAAGACAGTGAAGATGAGGAAGACGAAGGGGACAGTGAGGGCAGCGATGGGGACGAG GAGGACTACAAGCTATCGGACGAGAAGGACTTGGGGAAAGCTGGAGACACGAAGCCCAACAAAGACGCTA GCTCAGACTCCGAGTATGACTCTGATGACGATCGGACTAAAGAAGAGCGGGCGTACGACAAAGCAAAACG GAGAATTGAGAAACGACGACTTGAACATGGTAAAAATGTAAATACAGAAAAGCTAAGAGCCCCTATCATC TGTGTGCTTGGACATGTAGACACAGGGAAGACAAAAATTCTAGATAAGCTCCGCCACACTCATGTACAAG ATGGTGAAGCAGGTGGCATCACACAGCAGATTGGTGCCACAAATGTTCCTCTCGAAGCTATTAATGAACA AACTAAAATGATTAAAAATTTTGACAGAGAGAATGTCCGCATTCCAGGGATGCTGATTATTGACACTCCT GGACATGAGTCTTTCAGTAACCTGAGAAACAGAGGAAGCTCTCTCTGTGATATTGCCATTTTAGTTGTTG ACATTATGCATGGTTTAGAGCCCCAGACAATTGAATCAATCAACATTCTCAAATCCAAAAAATGTCCCTT TATTGTTGCACTTAATAAGATTGACAGGTTATATGATTGGAAGAAGAGTCCTGATTCGGATGTGGCTGTT ACTTTAAAAAAGCAGAAGAAGAATACAAAGGATGAGTTTGAGGAGCGTGCAAAGGCCATTATTGTAGAAT TTGCACAACAGGGTTTAAATGCTGCTTTGTTTTATGAGAATAAAGATCCCCGCACTTTTGTGTCCCTGGT ACCGACCTCTGCACACACTGGTGATGGCATGGGAAGCCTCATCTACCTTCTTGTGGAATTAACTCAAACT ATGTTGAGCAAGAGGCTTGCTCACTGTGAGGAGCTGAGAGCACAAGTCATGGAGGTTAAAGCTCTCCCAG GAATGGGCACCACAATAGATGTCATATTGATTAATGGACGTTTGAAGGAAGGGGACACAATCATTGTTCC TGGAGTAGAAGGGCCCATTGTCACTCAGATTCGAGGCCTCCTTTTACCTCCTCCTATGAAGGAATTACGC GTGAAGAACCAATATGAAAAGCATAAAGAAGTGGAAGCAGCCCAGGGAGTGAAGATTCTTGGAAAAGACC TTGAGAAAACGCTGGCTGGTTTACCTCTCCTAGTGGCCTATAAGGACGACGAAATCCCAGTTCTCAAAGA TGAATTGATCCATGAGTTAAAGCAGACGCTGAATGCTATCAAATTGGAAGAAAAAGGAGTCTATGTTCAG GCATCTACACTGGGCTCTTTGGAAGCTCTTCTTGAATTTCTGAAAACATCAGAGGTGCCATATGCGGGAA TTAACATTGGCCCAGTCCACAAGAAGGATGTTATGAAGGCTTCGGTGATGCTGGAACATGATCCGCAGTA TGCAGTAATTTTGGCCTTTGATGTAAGAATCGAACGGGATGCGCAAGAGATGGCTGATAGTTTGGGAGTT AGAATCTTTAGTGCAGAAATTATTTATCATTTATTTGATGCCTTTACAAAATACAGACAAGACTACAAGA AACAAAAACAAGAAGAATTTAAGCACATAGCAGTATTTCCCTGCAAGATGAAGATTCTCCCTCAGTACAT TTTCAATTCCCGAGATCCAATAGTAATAGGGGTCACTGTGGAGGCTGGTCAAGTGAAACAAGGAACACCT ATGTGTGTTCCAAGCAAAAATTTTGTTGACATTGGGATAGTAACAAGCATTGAAATAAACCACAAGCAGG TGGATGTTGCAAAAAAAGGACAAGAGGTCTGTGTCAAAATAGAACCTATCCCTGGGGAATCTCCAAAAAT GTTTGGGAGACACTTCGAAGCTACAGACATTCTTGTCAGCAAGATCAGCCGGCAGTCCATCGATGCACTC AAAGACTGGTTCAGAGACGAAATGCAGAAGAGTGACTGGCAGCTTATTGTGGAGCTGAAGAAAGTATTTG AAATCATCTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001110141
Insert Size	3651 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001110141.1, NP_001103611.1
RefSeq Size	4118 bp
RefSeq ORF	3651 bp
Locus ID	308306
UniProt ID	B2GUV7
Gene Summary	translation initiation factor; binds annexin V in a Ca2+ - and phospholipid-dependent manner [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.