Klhl20 (NM_001107192) Rat Untagged Clone
CAT＃: RN208727
Klhl20 (untagged ORF) - Rat kelch-like 20 (Drosophila) (Klhl20), (10 ug)
CNY 7790.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | RGD1309490 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN208727 representing NM_001107192
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGAAAGCCAATGCGCAGGTGTACCAACATTCGACCGGGAGAGACTGGAATGGATGTAACAAGCC GCTGTACCCTTGGAGACCCCAACAAACTCCCAGAAGGGGTTCCCCAACCTGCTCGCATGCCCTATATCTC AGACAAGCACCCTCGGCAAACCTTGGAAGTGATCAACCTCCTGAGAAAGCACCGAGAGCTATGTGATGTG GTGCTAGTTGTGGGCGCTAAGAAGATATATGCCCACCGAGTCATTCTGTCAGCCTGCAGCCCCTACTTCC GAGCAATGTTTACAGGAGAGCTAGCAGAGAGCCGGCAGACAGAAGTCGTGATCCGAGACATAGATGAGAG GGCTATGGAGCTACTGATTGACTTTGCATATACCTCCCAGATAACTGTGGAAGAGGGCAATGTTCAGACC CTCTTGCCTGCCGCTTGCCTCCTCCAGCTGGCTGAAATACAAGAAGCCTGCTGTGAATTCTTAAAGAGAC AATTAGATCCTTCTAATTGTCTGGGCATTCGGGCTTTCGCTGACACACATTCTTGCCGCGAGTTGCTAAG GATTGCAGACAAGTTCACCCAACATAACTTTCAAGAGGTAATGGAGAGTGAAGAATTCATGTTACTTCCA GCCAACCAACTCATCGATATAATATCTAGTGATGAGCTCAATGTTCGGAGTGAAGAACAAGTGTTTAATG CAGTGATGGCCTGGGTCAAATACAGTATTCAGGAAAGACGTCCTCAGTTACCCCAGGTGCTGCAGCACGT CCGCCTGCCTTTGCTTAGTCCCAAGTTCCTGGTGGGCACCGTAGGCTCTGACCCCCTCATTAAAAGTGAT GAAGAATGCAGAGACTTGGTAGATGAGGCTAAAAACTACCTCCTGTTGCCTCAAGAGCGACCTCTAATGC AAGGGCCTCGGACAAGGCCACGGAAACCCATCCGATGCGGAGAAGTGCTCTTTGCAGTTGGTGGTTGGTG CAGTGGAGATGCCATTTCCAGTGTTGAACGCTATGATCCCCAGACCAATGAGTGGCGAATGGTGGCTTCA ATGAGTAAAAGGCGATGTGGAGTCGGGGTCAGTGTTCTTGATGACCTGTTATATGCAGTAGGTGGCCATG ATGGATCCTCATATCTCAATAGTGTTGAAAGATATGACCCCAAAACAAACCAGTGGAGCAGTGATGTGGC CCCTACAAGCACCTGTAGGACAAGCGTTGGTGTGGCAGTCCTCGGAGGTTTTCTCTATGCTGTGGGTGGA CAGGACGGTGTCTCTTGCCTCAATATAGTTGAGAGGTATGATCCAAAGGAGAACAAGTGGACTCGGGTGG CTTCGATGAGCACTCGAAGGCTGGGCGTTGCTGTGGCTGTTTTGGGAGGGTTCCTGTATGCTGTAGGTGG CTCTGATGGGACATCCCCACTCAACACAGTGGAGCGCTACAATCCTCAGGAAAACAGGTGGCATACCATA GCGCCTATGGGGACCCGAAGGAAACACCTAGGTTGTGCAGTGTACCAGGACATGATCTATGCTGTGGGAG GGAGAGACGACACTACAGAGCTTAGCAGTGCTGAGAGATACAACCCCAGAACTAACCAGTGGTCTCCCGT GGTGGCCATGACATCCCGCAGGAGTGGAGTTGGCCTAGCCGTGGTCAATGGACAGCTCATGGCAGTTGGG GGTTTTGATGGTACAACATACTTGAAGACCATTGAAGTTTTTGACCCAGATGCCAATACATGGAGGTTGT ATGGAGGGATGAATTACCGTCGGCTAGGAGGTGGTGTAGGAGTTATTAAAATGACACATTGTGAATCCCA TATATGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001107192 |
| Insert Size | 1830 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001107192.1, NP_001100662.1 |
| RefSeq Size | 3134 bp |
| RefSeq ORF | 1830 bp |
| Locus ID | 304920 |
| UniProt ID | D3Z8N4 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity).[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR208727 | Klhl20 (Myc-DDK-tagged ORF) - Rat kelch-like 20 (Drosophila) (Klhl20), (10 ug) |
CNY 7790.00 |
|
| RR208727L3 | Lenti ORF clone of Klhl20 (Myc-DDK-tagged ORF) - Rat kelch-like 20 (Drosophila) (Klhl20), (10 ug) |
CNY 9690.00 |
|
| RR208727L4 | Lenti ORF clone of Klhl20 (mGFP-tagged ORF) - Rat kelch-like 20 (Drosophila) (Klhl20), (10 ug) |
CNY 10550.00 |
