Mei1 (NM_001130555) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208863 representing NM_001130555 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTGCTGACCATTCTGCGTTCGGGAGAGATGAAGAAGCCGTGCTGCTCCTGGAGAGGACTCATC ACCGGCACGATCCGCGCTGGTTGCTGCCGGTGTCCCCTCACGTCTGCCTGGCCTGCGCATTGGAGCTGCT GCCGGAGCCCGGCGTGTCGCTGGTGCGCAAGAAGCACGTGCTGTTCTGCTTCCAAGATGCCCTGGTGAGG CACACCTCCTTGGTCACCCAGCTGGTAGCTCAGGACCCCAGAGTCTGCATCCATTTTGTACGCGTGCTTT TCGGACTGTTAAGCAGTGTGGAAGACGGGAGTGTGGCAGACCTCTGTATTGAAGTCCTTATCCAGCTCAC TACTCAGCCCAATATGGAGCAGACCATCCAGTGCCTACTGAATGAGTGTCACAGAGAGCTGTGTAACCTG CCCTCCATGGGTGGCAGCCTGGCCACCACGACTCTTCTTGGCAAGCTGGTGAATGCCATTCCTGATTTGG CAGAAGATCTTGTCATGGAGTACGGCAACTTGATGGAGCATCTGTTGAGAGGCTTGGTTTACCCCAATGA GGGGGTGCAGGCTTCCATCTGTTACCTCTACGGGAAGCTCTACTCCTCGCCCACGGCTGCTGAGATGCTT TCCGGCCATTTCCGGGAGAAGCTGTGTGCCCTCTTCCTTTCCACTCTGGACAACGCCCAGACAAGGGACC TACAGATCAACTGCTTGGGTTTGCTAAGGCAGCTACTGAAGTACGATCTCTTTGTGTCCGTGATCATGAG CAAGTCTGTGCCGGTGGAGGGTGCCGAGAGTGTTGAGCAGCCATCGAGAGAGACCTCACTACCATTGGTG CTCAAAAAGTTTCTCCTGTCCAGAGATGAGGTCCTGCAGGTGGCCAGCTCCCACTGTATAACTGCAGTGC TGGTCCATTCCCCTGCAAAGCATGCTGTGGCCTTCATTTATGCTGACATCCCAGAGTTCCTCTTTGAGCA TCTTTCATCTTCCAGCGAAATCCTTGTCTGGTCCAGCTACAACTGCCTGATACTCCTGGCAGAAGAGCCA CTCTTCTTTTCAAAGTGTCACACGGTGTATGGGATCGAGGCCGTGGTCAGGAGCCTTCAGGGAAGCCTGC AGATGACCAACACAGAACTGCACAAGCAGGGCCTGCTGCTCTTCGCAGAAATCCTGACTCGGCAGCCTGA GGAGATCAGGCTGTTCACAAGCTCCGCCATGTGCAGAGATGCCAGCCGTGCCCTGCAGGAGGCCGTGAGC AGCCCTGTGTTAGCGGTGGCTGCTGAAGCACTGAGGGCCATTTCAGCTTTTCTGAGGAAGGACCACCAGA GCTCTCTGCCCGTGCAATACAGAGCCCTTCGTGCCTTGCTTGAAGCCATGCTGAGCCGATGTATGGAATT TTCCCAGACCCCTCTGAACAGGAGGTCCCTGGGCCACGCTTGCAGTAGAAACTTAGAGAAGGCCACTCTT CGAAAGGGGAAGTTCCTTCTGAGCACCCTGGAGGGGTTTAGAAACGCCTGCAGGTTGGCTGTGGAATTCC AAGGTGAGCCTTCTGCCCAGGAGAATCCCTTCACAGCTCCCAGCGCAGAGAAGGAAGACACCTTGGAGGC CTTCTCAGAGTTTCTTCTTAGTGCCTGTGACTCCCTGTGTATCCCCATGATGATGAGGTACTCGGAGGAG ACCACGCATCCAAACCTGATGGAGGTTTTCCTGTCAATTCTACACAGCCTCTTTGTCATCGTTCCCCACA TGAAGGTGAAGTTCTCCAGGAAGCTTGCTGATGCATCGTTCATACGACTGACCCTGGAGCTGAAGGCCAG GTTCTGCAGTGGCCAGAGTCACTCGTCCCTAAATCAGGTGTGCTCCAGCTTCCTCTACTACATGTGCCTC AATCTCCTCTTTGCTCCAGAGAAGACGGAATCACTTTCCCAAGAAGAACTCCCTGCAGTGTCTGAGTTCC TACAACATGGACTGCCCCACATAAGCAGCAGGACCCCAGAAAGTCTGGCCTTCCTGTCAGATCGGCAGCA TGTGGAGGTGGCCGCGCGCCAGAGACAGTACTGCATCCTGCTCCTCTTCTACCTTGCTCACATCCATGAT GACAGGTTTGTCCCTGATGCAGAGCTCTGTGTGGCTGTGCAGAGCTTCCTTTTGTCTCTGCAGGACCAGG GTGAGTGCCCTCCACCAGTGGTCTGCAAAGCTGCCATCTATCTGTTGGCGGTGTGCCAAGACAAGGACTG TGCACTGGGTGAGGCCGTGGTCAGTGCAATCAGAAAATGCCTAGAGGGCACCTCAGACCTGCGCCTGGTC TACACTCACCACCCGCTCCTGCTCAGGCTCTTCCTGGTGTGCCCAGGGCTGATGGGTAGGTTTGGGCACC GCGTCCTGGAGCTTTGGTTCTCTTGGGAAGAGAGCGGCTATGAGAACCTGGATGATGACTCGTCTCCGGG GCGAACCGTCTTTCCCGCCAACTTCGCAGCCCTGTTCCAGATGCTCAGGAGCATGCCCAGCGTCCTGCTC ATTCTGCTGGACCTCGTCTATTCCAGTCCTGTGGACACAGCCCACAAGGTTCTGATTGTCCTGAGGGCCT TCCTTCGGGAGAATGAAGACATTGAAGTGGGCGGTCTCATCCGAGGCCACTTCCTGCTCATCCTGCAGCG CCTCTTGGTGGAGTACGGAGCCTCCCCCTCAGGAGCCTCTGGGAACCTGCCGCTGCTGCTGAACCTCCTC TCCTCGCTGCAGCTGAGGACTGAGTCGGAACAGGAACTGGACAGCATGGCCATGAAGCTGCTTCACCAAG TGAGCAAGCTGTGTGGGAAGTGCAGCCCTGCAGATGTGGACATCCTGCAGCCCTCCTTCAACTTCTTGTA CTGGAGTCTTCATCGGACCACACCCAGTAGTCAGAAAAGAGCTGCTGCTGTCCTGCTGAGCAACACAGCC TTGATTGAGCTTCTGGAGAAGATGCTGGCCCTCACCTGGACAGAGGCAGACTCCTCTCCCAGGACGGCAC TGCTCTGCTCTGCCTGGCTGCTCACTGCCTCTTTCTCCGCCCAGCAGAACAGTGGCAATTTGCAGGTCCA TCAGACATTATCTGTGGAACTGAACCAAGTATTGAAGGCTCTCAGCTTTCCAAAAAAGTCGGCATTGCTG TCAGCTGCCATCCTACGATTCCTGCGGACGGCCTTGCAACAAAGCTTTTCCTCTGCCTTTGTGGCCTGGG TACCCTCAGGGGGTCAACCACTGTCAGCTCCTGAAGATGATGTCCTAGCTCCACTGGGAACATCACAAGT GCTATCCCTGTTGATTGGGCTCCAGAACCTCCTGGTACAGAAAGACCCTCTGTTATCTCAAGCTTGTATT GGCTGTCTGGAAGCCTTGCTCGATTACCTGCACGCCAGGAGCCCAGACGTCGCACTCCATGTGGCCTCCC AGCCCTGGAATCGGTTTTTGCTGTTCACCCTCTTGGATTCTGGAGAGAATGCCTTCCTCAGACCTGAAAT ACTGAGACTTATGACCTTGTTCGTGCAGTACCGGAGCAGTGGCATCCTCTCTCGTGAAGAGGTGGCTCTC ATTTTGCAAGGTGCAGCTTTGGCTGACCTGTCAACTCTCTCAAAAGATACACTCCAGGCTCTGCATGGCT TCCTCCTGCAGGTACAGCGCATCGGTCTCCTAAATGACAACAACATGACCCAGACCTTGCAAGCCTCCTT GGAGGGCCTTTGCTCCCGCACCTTCCCAGCCCAGCCAGTCTTTCAAGACATGCTTTGCCTTGGAGGTGTG TCTGTTTCCCTGTCTCACATCAGAGACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001130555
Insert Size	3810 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130555.1, NP_001124027.1
RefSeq Size	4018 bp
RefSeq ORF	3810 bp
Locus ID	315162

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