Zbed4 (NM_001134800) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209328 representing NM_001134800 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATAAGCAGGAAGTTTGTCCTGAGGGGGACAGGGATTTTGTTTCTGACAAAGTAAATTTCAAAA TAGAGGAGGAAGATGAAGATCAAATTCCCTGTCACAGTTTGCAACGCCGAATGGACTTTAAAAGTGAGTC AGAGGATATGAGACAGACAGACAGTGGCGATGAACAGGCAGAAATCAGGGCAGCAAGCTGTGCCTGTCAG CCCTCTGGGAAATTCTTACCTGCCGAGAGTGAGGATGACTATGGGTCACTCTTCTCCCAGTATAGTAGCA CACTGTACAACGTAGCCATGGAAGCCGTGACCCAGAGCCTTCTTTCCAGTCGACACATAAGCTCCAGGAA GAAGTCTCCGGCTTGGAAGCATTTCTTTATTTCCCCTCGAGATAGCACTAAAGCAATATGCACATACTGT ATGAAAGAGTTCAGTAGGGGCAAAAATGAAAAAGACTTGAGTACAAGTTGCCTCATGAGACACGTGAGGC GGGCACACCCCACCAAGCTCATTCAAGAAAATGGAAGTCTATCAGCAGGGTCCTCCTTTTCCTCTCCCTC TCTTCTGCTTCCACCACAGCCTGCAGATGTGGGAGACCTCAGCACTATGCTCTCACCTGTCAGACTTGTC CAGAAAATGGCTGCCAAGATCCCATCCCCTGACCAAATAATGGAAGAGTCTGTGACTGTAGTCTCTTCTG AAGAGGTGTCCTCTGATGTACCTATCACTGAAAAGTATAACAGAGAGGAGGCTTTGGCAGGCTCCTCCCC CAACCTCTCCATGCTCCATTATGATGACACTTCAGAAACCATAGCAGACAGAAACCTTTCCCTTCCCAAG AGCACATCTGGGTCCAGGAGGAGGTCAGCTGTCTGGAAGCACTTCTACTTGTCACCCCTTGATAGCTCCA AGGCTGTCTGTGTCCACTGTATGAATGAGTTCAGTAGGGGGAAGAACGGGAAGGACCTGGGCACCAGCTG CCTCATCAGGCACATGTGGAGAGCACATCGGTCCATTGTACTGCAGGAGAACGGGGGCAGCACAGGCATC CCTCCACTGTACCCTATGCCCCCGACCCTGCTGCCTGCCCTGCTGCCCCCAGAAGGGGATGTCAACTCTG CATCATTGTCTCCAGGCAAAGACGTGAAAGAGCCCCCTTCTGCCTCCTCATCCCCAGACAGGCTTCCCGA GGACTTGTCATCACACACCAATCCTGGGGATGTGTCCCGGGAAGATGTGTCCATGTTGTCATCCTCTGAT GACTTTGGGGAGGCCTCTGTAGTGTCCTCTCCAGAGAAGCGGCCAGCAGACACAGTAAATCCAAGGTTTG AATCAGGCACGGTCTTCCAGCAAAACAAGAAGGTGATGAGAAGACTGAAGTCAGAAGTCTGGCATCACTT TTCCCTGGCCCCCATGGACAGTCTCAAAGCAGTGTGCCGGTACTGCAGCTGCGTTATTAGTCGGGGGAAG AAGGGGGATGTTGGCACCAGTTGTTTGATGCGACACCTGTACAGACGCCACCCTGAGGTTGTTGGGAACC AGAAGGACTTTCTGGGTACAAGTTTGGCAAACTCTCCATACGCCACCTTAGCTTCTGCAGAAAGCTCCTC CAAGTTAACTGACTTGCCAGCAGCGATCAGAAAAAACCATCAAGGTATGTTTCCTACTAATAGCAAGAAG ACCTCAAAGCTGTGGAATCACTTCTCTATCTGTTCGGCAGACTCAACAAAGGTGGTGTGCCTGCACTGTG GCCGGACCATCAGCAGGGGCAAGAAGCCCACCAACCTGGGCACCAGCTGTCTCCTGAGACACCTGCAGCG GTTCCATGGCCATGTACTCAAGAATGATGTCTCAGAGGCCACTCTGCCCCATTCTCCAGGCATCCGGAGG CCTCTGGGCATGGAGCTCTCAGGGGCTTCCTCTTTGCGTGACTCCACAGAGAAGTTCTATGACTCTCATC CAGTTGCCAAAAAAATCACAAGTCTCATAGCTGAAATGATTGCACTTGACCTTCAGCCATACTCCCTTGT AGACAACGTTGGCTTTAACAGGCTGCTTGAATACTTGAAACCTCAGTACTCCTTACCCTCCCCTTCCTAC TTCTCCAGAACAGCCATCCCAGGTATGTATGACAATGTGAAACAGATAATTATGTCACATCTGAAGGAAG CTGAGAGCGGCGTGGTCCATTTCACCTCTGGAATCTGGATGAGTAGCCAGACTCGTGAATATCTGACCCT TACAGCTCACTGGGTCACCTTCGCATCCGCAGTCCGACCACATTGTGAGGACCACCATTGCTCAGCACTT TTAGATGTGTCACAGATCGACTGTGACTACAGTGGAAACAGCATCCAGAAGCAGCTGGAGTGTTGGTGGG AAGCCTGGGTGACTTCCATTGGTCTTCAGATTGGCATCACTGTCACTGACAACCCAAGCATAGGGAAGAT GCTGAGTGAGGGTGAACACTCAAGTGTGCAGTGCTTCAGTCACACAGTGAATCTGATTGTGAGCGAGGCC ATCAAGAGCCAGAGGATGGTGCAGAACTTACTGAGTATTGCCCGGAAGCTATGTGAGCGGGTCCATCGGT CACCCAGAGCAAGAGAGAAGTTAGCAGAACTGCAGAGAGAGTACGAGCTGCCCCAGCACCAGCTGATCCA GGATGTACCGTCCAAGTGGAGCACATCATTCCACATGCTTGAACGTCTAATTGAGCAGAAGAGGGCAGTC AACGAGGTATCCATTGAGTGCAACTTCAGAGAACTGATCAGCTGTGACCAGTGGGAGGTCATGCAGTCGG TGTGCCATGTGCTGAGACCATTCGATGCTGCAAGCCGAGAGATGAGTGCCCACATGTCTACACTGAGCCA AGTCATCCCCATGATCCACATCCTCAGCAGAAAAGTTGAGATGCTATTTGGGGAGACAATGGGCATTGAC ACCATGCTTAAGTCCCTGAAGGAAGCCATGGCGAGCCGCCTGTCTGCCACCCTCCATGACCCCAGGTACA TCTTTGCCACACTGCTGGACCCTCGCTACAAAGCTTCCCTGTTCACGGAGGAGGAGGCAGAGCAGTATAG GCAAGACTTAATCAGGGAGCTAGAAATACTGAATTCTACCTCAGAGGACACAGCCGCCTCCAATGGCTGT GACTCAGGGTCCCCACTTAAAGACACTGGCACAGAGGAGAGCCTGTGGTCACTCGCACCAATAAAGAGAG ATCAGAGAGAGAAGCTACCTGAAGACATGGTGCTTGCATATTTGGAGGAAGAGGTGCTGGAGCACAGCTG TGACCCACTCGCCTACTGGAACCTGAAGCGGTCATCCTGGCCTGGGCTGTCCACCTTAGCAGTGCGATTT CTGGGCTGTCCGCCAAGTACAGTCCCCTCAGAAAAGCTGTTCAGCACACCCATGGAGGCTGGCAGCTTTG GCCAGCCCAGGCTCATGATGGAGCATTTTGAAAAGCTCATCTTTTTAAAAGTGAATCTCCCCTTAATATG CTTTCAGTATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134800
Insert Size	3513 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134800.3, NP_001128272.1
RefSeq Size	5283 bp
RefSeq ORF	3513 bp
Locus ID	315211

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