Tbc1d9 (NM_001134539) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1308221
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209535 representing NM_001134539 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGTGAATCCCGAGGAGGTGCTGCTGGCCAACGCGCTGTGGATCACTGAGAGAGCCAACCCCTACT TCATTCTGCAACGAAGGAAGGGCCACGGGGGCGACGGAGGCGGCGGCGGCGGATTGGCGGGCCTCCTCGT GGGTACCCTTGACGTAGTATTGGACTCCAGTGCCCGTGTCGCACCGTACCGAATCCTCTATCAGACTCCG GACTCTCTAGTCTATTGGACCATCGCCTGTGGTGGTTCCAGGAAGGAGGTTACCGAGCACTGGGAATGGC TCGAGCAGAATCTACTGCAGACGCTCTCCATCTTTGAGAACGAGAATGATGTCACCACATTTGTAAGAGG AAAAATACAGGGCATCATTGCAGAATACAACAAGATCAACGATGTAAAAGAAGATGAGGACACAGAGAAG TTTAAAGAGGCCATCGTGAAGTTCCATAGACTGTTTGGGATGCCCGAGGAAGAGAAACTCGTCAACTATT ACTCCTGTAGCTACTGGAAGGGGAAGGTCCCCCGGCAGGGCTGGATGTACTTAAGCATCAACCACCTGTG TTTTTCCTCCTTCCTGATGGGGAGGGAAGCCAAGCTGGTGATCCGGTGGGTGGACATCACTCAGCTGGAG AAGAACGCCACGTTGCTTCTGCCGGATATGATCAAAGTGAGCACACGGTCCAGTGAGCATTTCTTCTCTG TGTTCCTCAACATCAATGAGACATTCAAGCTGATGGAACAGCTTGCCAACATAGCCATGAGGCAGCTCTT GGACAATGAAGGATTTGAACAAGACCGGTCCCTGCCAAAACTCAAGAAGAAATCACCCAAGAAAGTGTCT GCTCTGAAACGTGATCTGGATGCCAGGGCAAAGAGTGAGCGATACCGCGCCCTTTTTCGGCTGCCCAAAG ATGAAAAGCTGGACGGGCACACTGACTGCACCCTCTGGACCCCGTTTAACAAGATGCACATTTTGGGTCA GATGTTTGTCTCCACAAATTATATCTGCTTTACCAGCAAGGAGGAAAACCTCTGCAGCCTCATAATTCCA CTCCGGGAAGTAACCATCGTGGAGAAGGCTGACAGCTGCAGTGTGCTCCCCAGCCCTCTGTCCATCAGCA CCAGAAACAGGATGACCTTCTTGTTCGCCAACTTGAAAGACAGAGACTTTCTGGTGCAAAGGATCTCAGA TTTCTTGCAGCAAACGACTTCTAGAATCTACTCGGAGTTCTCAGGAAGCTGCAACAGCTCAGATGATGAG GTGTATTCTCGGCCCAGCAGCCTTGTTTCCTCCAGTCCCCAGAGAAGCACCAGCTCAGACGCTGATGGAG AGCGCCCCTTTAACCTTAACGGCAACAGCGTCCCCACAGCCACACAAACCCTCATGACCATGTACCGGCG GCGATCTCCTGAAGAATTCAACCCAAAATTGGCCAAAGAGTTTTTGAAAGAACAAGCCTGGAAGATTCAC TTTGCTGAGTATGGGCAGGGGATCTGCATGTACCGCACGGAGAAGACCCGCGAGCTGGTGCTAAAGGGCA TCCCAGAGCGCATGCGCGGGGAGCTCTGGCTGCTGCTTTCAGGGGCCATCAATGAAAAGGCCACACACCC CGGGTACTATGAGGACCTGGTGGAGAAGTCTATGGGAAAGTACAATCTTGCCACAGAGGAGATTGAGAGA GACTTACACCGTTCCCTTCCAGAGCACCCAGCTTTCCAGAACGAAATGGGCATCGCGGCTCTGAGGAGAG TCTTAACGGCTTATGCTTTTCGAAATCCCAACATAGGGTATTGCCAGGCCATGAACATTGTCACTTCAGT GCTGCTGCTTTATGCCAAAGAGGAGGAGGCCTTCTGGCTGCTGGTGGCCTTGTGTGAGCGCATGCTTCCG GATTACTACAACACCAGGGTTGTAGGTGCCCTGGTGGACCAGGGAGTCTTCGAGGAGCTGGCTCGAGACT ATGTCCCCCAGCTGTATGACTGCATGCAGGACCTGGGGGTGATCTCTACCATCTCCCTGTCATGGTTCCT CACGCTCTTTCTCAGTGTGATGCCATTTGAGAGTGCCGTGGTGGTCGTGGACTGTTTCTTCTATGAAGGA ATCAAAGTGATATTCCAGCTGGCCCTCGCTGTACTGGATGCCAATGTGGATAAACTGTTAGGCTGCAAGG ACGATGGGGAGGCTATGACTGTTCTGGGAAGGTACTTAGACAGCGTGACCAACAAAGACAGCACTCTGCC ACCCATCCCTCATCTCCACTCCCTGCTCAGTGACGACGTGGGGCCCTACCCTGCGGTGGACATCTTCAGA CTCATAGGAACATCCTACGAGAAATTTGGAACTATCCGGGCAGATTTGATTGAGCAAATGAGATTTAAGC AGAGGCTGAAAGTGATCCAAACCCTGGAGGACACCACCAAACGCAATGTGGCTGAACATCTGACCAGCTG CTACTGGGGTGGGAGCAGCAATGCCCCGGACCGGCACGACCCCAGCCTGCCTTACCTGGAGCAGTACCGC ATCGACTTCGAGCAGTTCAAGGGCATGTTTGTGCTCCTCTTTCCCTGGGCGTGTGGCACTCATTCTGATG TGCTGGCCTCCCGCTTGTTCCAGCTACTGGACGAGAATGGGGACTCCTTGATTAACTTCCGAGAGTTCGT CTCTGGGCTGAGTGCTGCGTGCCATGGGGACCTCACAGAGAAACTCAAACTCCTGTACAAAATGCACGTC TTGCCTGAGCCATCCTGCGATCAAGACGAGCCCGACTCTGCCTTTGAAGCGACCCAGTACTTCTTTGAAG ATATCACCCCAGAATGCACACATGTTGTTGGCTTGGACAGCAGAGGCAAGCAGAGTGCTGATGAGGGTTT CGTCACCGTGAGTCTGAAGCAGGACAGAGGGAAAAGGGCAAATTCTCAAGAGAACCGCCACTATCTAAAA CTCTGGACTGCAGAAAATAAGTCAAAGTCAAAAAATGCCAAAGATTTGCCCAAGTTGAATCAGGGGCAGT TTATCGAGCTCTGTAAGACCATGTACAACATGTTCAGTGAAGACCCCAATGAACAGGAGCTGTACCACGC CACGGCTGCGGTGACCAGCCTCCTACTGGAGATCGGAGAGGTCGGCAAGTTCTTCATTACTCAGCCCGCC AAGGAAGATGCGGTTCCTGGACCACCCTGCAGCCAGGCCATCCCCGGCATGCTCTTCCCCAAGAAGGGGT CCAGCCAGTCCTATGTGGTGGAATCCACAGACCCACTCCCAGCTAGCCTGGCGGGTGACAGGGAGGAGCA CTCCCTGGGAGGACAGATGGAGGACATTAAACTAGAGGACTCCTCACCCAGGGACAACGGGGCCTGCTCC TCCATGTTGATCTCCGATGACGACACCAAGGACGACAGCTCCATGTCATCCTACTCGGTGCTGAGTGCCG GCTCCCACGAGGAGGACAAACTGCACTGTGAGGACATTGGGGAGGACACTGTCCTGGTGCGAAGCAGTCA GGGGAGAGCTGCCCTGCCCAGGAGCAGCAGCCTGGACCGGGACTGGGCCATTACCTTCGAGCAGTTCCTA GCCTCGCTCCTGACGGAGCCTGCTCTCGTGAGGTACTTTGACAAGCCGGTGTGCATGATGACCAGGGTCA CCAGTGCGAAGAATATCCGTATGATGGGCAAGCCCCTCACCTCAGCCAGTGACTACGACATCTCTGCCCT GTCAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134539
Insert Size	3720 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134539.1, NP_001128011.1
RefSeq Size	5730 bp
RefSeq ORF	3720 bp
Locus ID	304645

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