Tcf20 (NM_001130574) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	AR1; SPBP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209738 representing NM_001130574 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTCGTTTCGGGAGCAAAGCAGTTACCACGGAAACCAGCAGAGCTACCCACAGGAGGTGCACAGCT CATCCCGAATAGAAGAGTTCAGCCCTCGTCAGGCCCAGATGTTCCAGAATTTTGGGGGAGCAGGTGGTGG TAGCAGTGGCACTGGCAGCAGCAGTAGTGGACGGCGAGGAACAGCTGCGACAGCGGCAGCAATGGCTAGC GAGACCTCTGGCCATCAAGGCTATCAGGGTTTCAGGAAAGAAGCTGGAGATTTTTACTACATGGCAGGCA ACAAAGATACCGTGGCAGCAGGAACTCCACAGCCTCCTCAGCGAAGGCCTTCTGGGCCTGTGCAGAGCTA TGGACCTCCCCAGGGGAGCAGCTTTGGCAATCAGTATGCAAGCGAGGGTCATGTGAGCCAATTTCAAGCA CAGCACTCTGCCCTTGGTGGCGTGTCTCATTATCAGCAGGATTACACAGGGCCTTTCTCTCCTGGGAGTG CTCAGTATCAACAGCAGGCCTCTAGCCAACAACAGCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCA ACAGCAACAGCAGCAACAGCAACAGCAGCAAGTACAGCAGTTGAGACAACAGCTTTACCAATCACATCAG CCTCTGCCACAAGCCACTGGACAGCCAGCCTCTGGCTCATCCCATCTACAACCAATGCAGCGGCCCTCAG CTCTGCCATCTTCTGCTGGTTATCAGTTAAGAGTGGGTCAGTTTGGGCAGCACTACCAGTCTTCTGCTTC CTCTTCTTCCTCCTCCTTTCCTTCACCACAGCGTTTCAGTCAGTCTGGACAGAGCTATGATGGCAGTTAC AGCGTGAATGCTGGATCTCAATATGAAGGGCATAATGTGGGTTCTAATGCACAGGCTTATGGGACACAGT CAAATTATAGCTATCAACCTCAGTCTATGAAAAATTTTGAACAGGCAAAGATTCCACCAGGATCCCAGCA GGGGCAGCAGCAACCACAGCAGCAGCCACAACCTCAGCAGCAGCAGCAGCAGCAGCCGCCGCAGCAGCAA CAGCAGCAGCAACATCCCCCCCAGCATGTGATGCAGTACACAAATGCTGCCACCAAGCTGCCTCTGCAAA GCCAGGTGGGGCAGTACAACCAGCCTGAGGTTCCTGTAAGGTCCCCTATGCAGTTTCACCAGAACTTCAG CCCTATTTCTAACCCTTCTCCAGCTGCTTCTGTGGTTCAGTCTCCAAGCTGTAGCTCTACCCCTTCTCCT CTCATGCAGAGTGGTGAGAATCTCCAGTGTGGGCAAGGCAATGTATCAATGAGTTCCCGAAACCGAATTT TACAGTTAATGCCTCAACTCAGTCCAACTCCATCAATGATGCCCAGTCCTAATTCTCATGCTGCAGGATT CAAAGGGTTTGGATTAGAAGGGGTGCCGGAAAAGCGGCTGACTGATCCTGGGTTGAGTAGCTTGAGTGCT CTGAGTACTCAAGTGGCCAATCTTCCTAATACTGTCCAGCATATGTTACTTTCTGATGCCTTGACACCTC AGAAGAAGACTTCTAAGAGGCCCTCATCATCATCTAAGAAAGCAGATAGCTGTACAAACTCGGAAGGCTC CTCACAGCCTGAAGAACAACTAAAGTCCCCCATGGCAGAGTCATTGGATGGAGGTTGCTCAAGTAGTTCA GAAGATCAAGGTGAGAGAGTGAGGCAACTAAGTGGCCAGAGCACTAGCTCTGACACCACCTATAAGTGTG GAGCTTCAGAGAAAGCTGGCTCCTCACCTACGCAAGGTGCTCAGAATGAGGCCCCTAGGCTCAGTACCAG TCCTGCAACTAGGGAAGAAGCTGCCTCTCCAGGTGCTAAGGACACATCACTGTCATCTGAGGGGAACACA AAAGTCAACGAGAAGACAGTTGGGGTGATTGTCTCCCGAGAAGCCATGACAGGCCGGGTAGAAAAATCTG GTGGACAAGATAAAAGCTCCCAGGAGGATGATCCTGCTGCCAGTCAGAGACCACCTAGCAATAGTGGTGT AAAGGAAATGAGCCACACATCACTTCCACAGTCAGATCCTCTAGGAGGCGGGAGCAAGGGAAACAAGAAT GGTGATAATAATAGCTCTAACCACAATGGAGAAGGGAATGGCCAGAGTAGCCACTCTGCAGTAGGCCCAA GTTTCACAGGCAGGACTGAGCCTAGCAAGTCTCCTGGAAGTTTGCGCTATAGTTACAAAGAGAGTTTTGG GTCAGCTGTACCACGAAATGTCAGTGGTTTTCCTCAGTATCCTTCTGGACAAGAAAAGGGGGATTTTGGC AGTCATGGGGAGCGAAAAGGTAGAAATGAGAAGTTCCCAAGTCTCCTACAGGAAGTGCTTCAGGGTTACC ACCACCATCCTGACAGAAGGTATCCTAGAAGTGCTCAGGAACATCAAGGGATGGCTAGTGGCCTGGAAGG AACTGCAAGGCCCAACATCTTAGTCAGTCAAACCAATGAATTAACCAGCAGGGGCCTTCTGAACAAGAGT ATTGGATCCTTGTTGGAAAATCCCCACTGGGGACCATGGGAAAGGAAGTCAAGCAGCACAGCTCCTGAAA TGAAGCAGATCAATTTGTCTGACTATCCCATTCCCAGAAAGTTTGAGATAGAACCTTCATCATCATCCCA TGAGCCTGGGGCCTCCCTTTCTGAAAGGAGGTCAGTAATCTGTGATATTTCCCCACTAAGACAGATTGTC AGGGACCCAGGGGCTCACTCTCTGGGACACATGGGTGCTGATGCCAGAATTGGAAGGAATGAACGTCTCA ACCCAAGTTTAAGTCAGTCAGTCATTCTTCCTGGTGGATTGGTGTCCATGGAAACAAAGTTGAAATCCCA GAGTGGGCAGATAAAAGAGGAAGACTTTGAACAATCCAAATCTCAAGCTAGTTTCAACAAGAAATCTGGA GACCACTGCCACCCTACCAGCATCAAGCATGAGACTTACCGTGGCAATGCCAGTCCTGGAGCAGCAGCCC ATGATTCCATTTCAGACTATGGCCCACAAGATAGCAGGTCCACACCAATGCGGCGGGTCCCTGGTAGAGT TGGTAGCCGGGAGACTATGAGAGGTCGTTCCTCTTCTCAGTACCATGACTTTGCAGAAAAATTGAAGATG TCTCCAGGCAGGAGCAGAGGCCCAGGGGGAGACCCTCATCACATGAACCCACATATGACTTTTTCAGAGA GGGCCAATAGGAGTTCTTTACATGCTCCTTTTTCTCCCAACTCAGAAAGCCTGGCCTCTGCTTACCACAC AAATACCAGGGCTCATGCTTATGGGGACCCTAATACTGGTTTGAATTCCCAGCTTCATTATAAGAGACAG ATGTACCAGCAGCAACAAGAGGAGTATAAAGATTGGGCCAGCAGTTCTGCTCAGGGAGTGATTGCTGCTG CACAACATAGGCAGGAAGGGCCACGGAAGAGCCCACGGCAGCAGCAGTTTCTTGACAGAGTACGGAGCCC CCTGAAAAATGACAAAGATGGTATGATGTATGGCCCACCAGTAGGTACATACCATGACCCAAGCACTCAG GAAGCTGGGCGCTGTCTCATGTCTAGTGATGGCATGCCTGCCAAAAGCATGGAATTGAAGCACAGCTCTC AGAAGTTACAAGAGTCTTGTTGGGATCTTTCTCGGCAGACTTCTCCAGCCAAAAGCAGTGGACCGCCAGG AATGTCTAATCAAAAACGGTATGGGCCACCCCATGAGCCGGATGGACATGGACTAGCTGAGTCTACACAG TCATCCAAACCTAGTAATGTAATGTTAAGGCTTCCGGGTCAAGAGGATCATTCTTCTCAAAATCCCTTAA TCATGCGGAGGCGGGTTCGTTCTTTTATCTCCCCTATTCCCAGTAAGAGACAGTCACAAGATGTAAAAAA CAGCAACACTGATGATAAAGGGCGCCTCCTCCACCCATCAAAGGAAGGTGCCGATAAAGTGTACAATTCC TACAGCCATCTTTCTCACAGTCAGGATATCAAGTCTATCCCTAAGAGAGATGCCTCCAAGGACCTTCCAA ACCCAGACAATAGAAACTGTCCTGCTGTTACCCTGACAAGCCCTGCTAAGACCAAAATACTGCCCCCACG GAAGGGACGGGGATTAAAATTGGAAGCTATAGTTCAGAAGATCACATCCCCAAATATTAGGAGGAGTGCA TCTGCAAACAGTGCTGAGACTGGGGGAGACACAGTCACACTGGATGATATACTATCTCTAAAGAGTGGTC CTCCGGAAGGTGGGACTGTGGCTAATCAAGAGGCTGAGATGGAAAAGCGAAAGGGTGAGGTGGTATCTGA CCTAGTCAGTGCAACTAACCAGGAATCGAATGTTGAAAAACCTCTTCCAGGGCCTTCCGAAGAGTGGCGT GGCAGTGGGGATGACAAAGTCAAGACAGAGGCACATGTAGAAACAGCTTCTACTGGAAAAGAACCCTCTG GTACTATGACATCCACAGCTTCACAGAAGCCTGGTGGTAACCAAGGGAGACCAGATGGTTCCCTGGGTGG GGCAGCACCTCTAATCTTTCCTGACTCAAAGAATGTAGCTCCAGTGGGCATATTGACCCCTGAGGCAAAC CCCAAGACTGAAGAGAAGGAGAATGATACAGTCATGATTTCACCCAAGCAAGAAAGTTTCCCCCCAAAAG GGTATTTCCCATCAGGAAAGAAAAAGGGGAGACCAATTGGTAGTGTGAATAAACAAAAGAAACAACAGCA GCAACCACCTCCACCTCCTCAGCCCCCTCAGATGCCAGAAGGTTCTGCAGATGGAGAGCCAAAGCCAAAA AAGCAGAGGCAAAGGAGGGAGAGAAGGAAGCCTGGGGCCCAGCCAAGGAAGCGGAAAACCAAGCAAGCAG TTCCCATTGTAGAACCCCAAGAACCAGAGATCAAGCTAAAGTATGCTACCCAGCCACTAGATAAAACTGA TGCCAAGAACAAGTCATTTTTCCCTTATATCCATGTAGTAAATAAGTGTGAACTTGGAGCTGTTTGTACA ATCATCAATGCTGAAGAAGAAGAACAGACCAAATTGGTGAGGAGCCGGAAGGGTCAGAGATCTCTGACCC CTCCTCCCAGCAGCACAGAAAGCAAGGTGCTCCCAGCTTCATCCTTTATGCTGCAGGGGCCTGTGGTAAC AGAATCTTCTGTTATGGGGCACCTGGTTTGCTGTCTTTGTGGCAAGTGGGCCAGTTACCGTAACATGGGT GACCTCTTTGGACCCTTTTATCCCCAAGATTATGCAGCCACTCTTCCGAAGAATCCACCTCCTAAGAGGT CCACAGAAATGCAGAACAAGGTCAAGGTTCGGCACAAAAGCGCTTCTAATGGTTCTAAAACTGACACCGA GGAGGAGGAGGAGCAGCAGCAGCAGCAGAAGGAGCAGAGGAGCCTAGCTGCTCATCCTAGGTTCAAGCGG CGCCACCGCTCCGAAGACTGTGGTGGAGGTCCTCGGTCCCTGTCCAGGGGGCTCCCTTGTAAAAAAGCAG CCGCTGAGGGCAGCAGTGAAAAGACTGCTTCAGACACAAAGCCCTCTGTACCTACCACTTCAGAAGGCGG TCCTGAGCTGGAGTTACAAATCCCTGAACTACCTCTTGACAGCAACGAATTTTGGGTCCATGAGGGTTGT ATTCTCTGGGCCAATGGAATCTACCTGGTCTGTGGCAGGCTCTATGGCCTGCAGGAAGCGCTGGAAATCG CCAGAGAGATGAAATGCTCCCACTGCCAGGAAGCTGGCGCTACTCTGGGCTGCTACAACAAAGGCTGCTC CTTCCGATACCATTACCCGTGTGCCATTGACGCAGATTGTTTGCTGCATGAGGAGAATTTCTCGGTGAGG TGCCCCAAGCACAAGGTGAGACTGTGGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001130574
Insert Size	5913 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130574.1, NP_001124046.1
RefSeq Size	6502 bp
RefSeq ORF	5913 bp
Locus ID	366964

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