Lamc3 (NM_001107830) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210588 representing NM_001107830 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGTGTCCGGGATCCTGTCCCGCCTGGCAACGGTGGTATGTGTGGCGTTGGTGATGCTGAAGGCGC ACTGCGCGACGGGTGCGGGCATGGGCTCTTGCTACGACGGTGCGGGACGCGCACAGCGCTGTCTGCCTGA GTTCGAGAACGCGGCGTTCGGCCGACGCGCCGAGGCCTCGCACACGTGCGGACAGCCCCCAGAGGACTTC TGTCCGCACGTGGGGGCACCAGGAGCTGGGCCACAGTGCCAGCGCTGTGATGATGCTGACCCCCGGCGAC GCCACGACGCCTCCTACCTCACAGACTTCCACAGCCCTGATGACAGCACCTGGTGGCAAAGTCCGTCCAT GGCCTTCGGGGTGCAGTATCCCACTTCGGTTAACCTGACCCTGCATCTAGGGAAGGCCTATGAGATCACC TATGTGAGGCTAAAGTTCCACACCAGCCGCCCCGAGAGCTTTGCCATCTACAAGCGCACGCACACGGGTG GCCCCTGGGAGCCCTACCAATACTACAGTGCCTCCTGCCAGAAAACCTATGGCCATCCTGAGGGCCACTA CCTGCGACCTGGTGAAGATGAGAGGGTGGCTTTCTGCACCTCTGAGTTCAGCGACATCTCCCCCCTGAAT GGGGGCAACGTGGCCTTCTCCACTCTGGAGGGCCGTCCCAGTGCCTACAACTTTGAGGAGAGCCCTGTGC TGCAGGAGTGGGTTACCAGCACCGATCTCCTGATCTCTCTGGATCGTCTCAACACGTTTGGGGATGACAT CTTCAAGGACCCCAGAGTGCTCCAGTCTTACTACTACGCTGTGTCTGACTTCTCTGTGGGTGGCAGGTGC AAATGCAATGGTCACGCCAGTGCGTGCGGGCCCAACGAGGCTGGTCAGCTGGCTTGTCACTGCCAGCACA ACACCACGGGAGTGGACTGTGAGCGTTGTCTACCCTTCTTCCAGGACCGTCCGTGGGCCCGCGGCACCGC AGAGGATGCCAACGAGTGTCTGCCCTGCAACTGTAGTGGGCACTCTGAGGAGTGCACGTTTGACAGGGAG CTCTATCGGAGCACGGGCCATGGTGGGCACTGCCAGCGCTGCCGAGACCACACAGCTGGGCCACATTGTG AGCATTGTGAGAAGAACTATTACAGATGGGACCCGAAGACACCATGCCAACCCTGTGACTGCCACCCAGC AGGCTCCCTGAGTCTCCAGTGTGACAACTCGGGCACCTGTCCCTGCAAGCTGACGGTGACTGGCTGGAAG TGTGATCGCTGCCTGCCTGGATTCCACTCACTCAGTGAGGGCGGCTGCAGACCCTGTACCTGCAATGTCG CTGGCAGCTTGGGCACCTGCGATCCCCGAAGTGGAAACTGCCCCTGCAAAGAGAATGTGGAAGGCGGTCT GTGTGACAGATGCCGCCCTGGGACTTTTAACCTGCAGCCCCATAACCCAGCCGGCTGCAGTAGCTGCTTC TGTTACGGCCACTCCAAGGTGTGTGCCCCTGCTTCAGGGTTCCAGGAACACCACATCCGCTCAGACTTCC GCCATGGAGCTGATGGCTGGCAGGCCAGAAGCACAGAGGTGTCAGAGCGTCCTCTGCAATGGAGCCAGAG CGGGATCCTCCTGGGCCTGCGAGGAGGGGAGGAACTCTCAGCCCCAGAGAAGTTCCTGGGAGACCAGAGA CTCAGCTATGGACAGCCACTCATACTGACCCTCCAAGTACCCCCCGGGGGCTCCCCACCTCCTATGCAGC TGAGACTGGAGGGGGCAGGTTTATCTCTGGCTCTGAAACCCTCCAATCTACCCAGCTCTCAGGACACCAG GCAGCCAGGACGAGTTCAGCTCCAGTTCCTCCTGCAGGAGACTTCTGAAGAGGCAGAGCCTCCACTGCCC GCCTTCCACTTCCAGCGCCTGCTTTCCAATCTGACCACTCTGAGCATCTGGACCAGTGGCCAAGGCCTGG GCCACTCTGGCCACGTGCTCTTGTGTGAAGTCCATCTCACATCGGCCCGGCCTCAGCGTGGGCTTGCCCC ACCAGCCTCTTGGGTGGAGACCTGCTTATGTCCCCAGGGATACACGGGCCAGTTCTGTGAATTCTGTGCT CTGGGATACAAAAGAGAAATACCTCATGGGGGTCCCTATACCAACTGCATCCCCTGCACCTGCAACCAGC ATGGAACCTGTGACCCCAACACAGGGATCTGCCTGTGTGGCCACCACACGGAGGGTCCATCCTGTGAGCG ATGCATGCCAGGTTTCTACGGTAACGCCTTCTCAGGCCACGCTGATGATTGCCAGCCCTGTCCATGCCCT GGCCAATCAGCCTGCACGACCATCCCAGAGAGTAGAGATGTGGTGTGCACACACTGCCCCCCTGGTCAGA GAGGACGACGCTGTGAGAGCTGTGAAGATGGCTTCTTCGGAGATCCCCTAGGACTCTCTGGAGCTCCCCA GCCCTGCCGCCGATGCCAGTGCAGCAGGAACGTGGATCTCAATGCTGTGGGCAACTGCGATCCTCATTCT GGCCGCTGTCTGCGCTGTCTGCACAACACAACAGGGGCCCACTGCGAGCACTGTCAGGAGGGTTTCTATG GGAGTGCCTTGGCCACGAGGCCTGCGGACAAATGTGCTCCCTGCAGCTGTGACCCGAGGGGCTCAAGCAG TCAGAAGACCTGCAACCCAGCGACTGGCCAGTGTGCCTGCCTGCCTTACGTCACCGGGAGGGATTGCAGC CGCTGCAGCCCTGGCTTCTACGACCTCCAGCCTGGGAGGGGCTGCCAGAGCTGCAAATGTCACCCAGTGG GCTCCTTGGAGAACAAGTGCCACCCCAAGACTGGCCAGTGCCCCTGTCGACCCGGTGTCACCGGCCAAGC CTGTGACAGATGCCAGCTGGGTTTCTTTGGCTTCTCCATCAAGGGCTGCCGAGACTGTAGGTGTTCCCCA CTGGGTGCTGCCTCACCCCAGTGCCATGAGAACAGCACGTGTGTGTGCCGGCCTGGCTTCGTGGGCTATA AATGTGACCGCTGCCAGGACAATTTCTTCCTCGTGGATGGTGACACAGGCTGCCAAGAGTGCCCCACCTG CTATGCTCTAGTGAAGGAGGAGGCAGCCAAGCTGAAGACCAGGCTGATGCTGATGGAGGGGTGGCTTCAG GGGTCCGAGTGTGGCAGCCCCTGGGGACCACTAGACATTCTGCAGGGAGAAGCCCCTCGGGGGGATGTCT ACCAAGGTCACCACCTATTTCAAGAGGCCCGGGGGACCTTCCTGGAGCAGATGGTGGGCCTGGAGGAGTC TGTGAAGGCCACTTGGGAGCAGCTGCAGGTGCTGAGAGGGAACGCACACTGTGCCCAGGCTGGAGCTCAG AAGACCTGCAACCAGCTGGCAGAGCTGGGGGAGACGCTGCGTTCCTCAGAGGAGGAGGTCCTGCGTGCAG CCTCAGCTCTCTCATTTCTGGCAAATCTTCAGGAAGGATCCAGCGCACCCACCAATTGGGGTCGCCTGGC ATCAGAGGCCTACATCCTCACCAGGAGCCACAGGGACACAGCCACCAAGATCGAAGCTACCGTGGAAAGG GCCCTGCTCGCCTCCAACACCAGCTATGAGCTCCTGTGGAAGCTGTTGAAAGGCAGTGTGGCCTCAGAGG CCCAGCAGGAACTGGAGGAGAGGTACCAGGAGGTGCAGGCAGCTCAGACTGCCCTGGGCATAGCTGTGGC AGAGGCGCTGCCCAAAGCTGAAAAGGCACTAGCCACGGTGAAGCAAGTCGTTGGTGCCGCAGCCCCACGT CTGGGCTCGATGGTCACCCCTGAAGCAATGGACTCCCAAGCTAGATACCTAGGCCGGAGAGTGAAAGCCC TGGAGCAGAAGCTGCAGCAGAACCAGGCAGGCCAAGCGGTGGGAGCCCTGCAGGTAGAGGCTGGGAGAGC CTTGAGGAAGGTGGAGCCCTTTCTGCAGCTACGAAACAAGACCACAGCTGCCCTGACACAGGTTTCCTCA GCTGTCCAAGCTGCTAAGGTGACCGTCATAGGAGCGAAGACCCTGCTAGCTGACCTAGAGGGAATGAAGC TAAGGTTCCCTCTACCCAAGGAGCAGGCAGCGCTGAAGAGGAAAGCAGGCAGCATCAGGACCAGGCTCTT GAAGGACACGAAGAGAAAGACCAAGCAGGCAGAGAGGATGCTGGGAAATGCTGCCTCGCTCTCCTCCAAC TCCCAGAAGAGAAGCAAAGAAGCAGAACTGATGTCCAAGGAGAATGCCAAGCTTGCCAGAGCTTTCCTGA GGGAAGGGAAGCAGAACTACCGTCATGCCAGCCGACTCGCCAGCCAGACCCAGGCCACACTCCGCCAGGC CTCTCGCCTGGTGCTGACCTCAGAAGCACGGAAACAGGAGCTGGAAGAAGCTAAACAGGTGGCCTCTGGG CTGAGCACTGTGGAGCGCCAGATCCGAGAGTCTCGGATCTCCTTGGAGAAGGACACCAAGGTCCTGTCAG AGCTGCTCGCGAAACTGGGGTCCCTGGGTACCCACCAAGCCCCTGCTCAGACCCTGAACGAGACCCAGCA GGCACTAGAAAGCTTGAGGCTGCAGCTGGATTCACATGGATCCTTGCATCACAAACTAAGGCAGCTGGAG GAAGAGTCTGCTCGACAGGAGCTGCAGATCCAGAGCTTTGAGAACGACCTCGCTGAGATCCGCGCTGACA AGCACAACTTGGAGACCATTCTAAGCAGTCTACCAGAGAACTGTGCCAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107830
Insert Size	4743 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107830.1, NP_001101300.1
RefSeq Size	6512 bp
RefSeq ORF	4743 bp
Locus ID	311862

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