Phrf1 (NM_139093) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210668 representing NM_139093 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGGCTGAAGAGGAAGACCCGACCTTTTGTGAAGTGTGTGGCAGGAGTGATCGTGAGGACCGGCT CCTACTTTGTGACGGCTGTGATGCTGGATGCAGCTCCTGTGAGTGATGAGGAGGTCTCCTTGCTACTGGC TGATGTAGTGCCAACCACCAGCAGACTTCGGCCTCGAGTAGGCAGGACCCGGGCCATCGCTAGGACACGG CAGAGTGAGAGAGTGAGGGCAACTGTGAACCGGAACCGGATCTCTTCTGCCAGAAGGGTCCAGCATGTGC CACGGTACCTCATGTCTTCTCTGCTGGATGAAACTATTGAGGCTGTCGCCACTGGTCTGAGCACAGCTGT ATACCAGCGTCCCTTGACACCTCGTGTTCCTGCAAAACGGAAAAGGAAGGCAGGGAGACGAAAGAAAGTA CTGGGAAGGAAGAAAACCCGGTCCAGGTCATCTGTGAAAAGTAAGAGTGGGGGTACAAGAGCCAAGAAAC GGCAGCACCGTGTGAGGAGGACGAAAGGGAGGAAGCTGAAGAACGAAGTCACAGCTCGTTCTCGCATTGC ACGAACACTAGGCCTCCGCAGGCCTGTCCGTGGCACCAGCATGCCATCAGTATACAAGCCTGTGGACCCC TCTCTTGGGCTGATGAGGGCAGATATTGGAGCAGCTTCTCTGTCACTGTTTGGAGATCCCTATGCACTAG ACCCTTTTGACAGCAATGGAGAACAGTCTGCAGATCCGCCTTCCCCTCTGAGTGCCAAGAGGAGAGTGCT GTCCCGCTCAGCCCTGCAGTCTCACCAGCCTGTGGCCAGACCTGTCGCCATGGGACTCGCTAGGAGGCAG CTCCCTGCTGTGGCCCCAGAGCCCAGTGTGGAGGAGGCCCCCGTCCCTGACCTGCTGGGGAGCATCCTGT GTGGCCAGAGCCTCCTGATGATGAGCAGTGCTGATGTTGTCATACACCGGGATGGCTCTCTCAGCGCCAA GAGGGCAGCTCCTGTTTCTCTTCAGCGAAATTCTGTGACTCAATCCAGAGAAGAGTCCAGGCTCAGAGAC AACCTGCAGCCTGGGGCACTACCTTCAGAGAGCGTATCCGGTGGACTCATAGGAGACAGACGACCAAACT CAGGGCTGAGCTGTGGGGACAGAACAGCTCTGCGCTGTCTCCCTGCCCAAATAGTGCAGACACCTGTGAG GTCAGATTCATCAGTTTCCCCTCGTTCAGGTCTGTCTGGGAACCTTTCAGATGAGAGCAGACCTAAATGG AAACACAGTAACAGCCCCCGACTCAATGGCTCCAATGTACGGGTTGGTTCTGCTTCAACTAAGACCATGA CTCATTCTAACTTTCCATCTAAAAATATAGCTCCTGGGCATCCTCAGAAAACAGATCCCAGGAGACCTGA TTTCTCCAAGCTCCCCAGGATACCAAAGATCCGCAGGGATGGCAGTAATAGCACACAGGACCAGGCCCCA GCCAGCGGTCAGACTGTCGAGCTCCCCAGCGCATGCATCAGCCGCCTGACTGGCAGGGAAGGCCCTGGGC AGCCAGGACGAGGCCGGGCTGACAGTGAGCCCAGCAGCAGGGGTCCCCAGGAGACTGGCTCACACACAAG TGGTTCTCGACCTCCTGCGCCCAGCTCCCATGGCAACCTAGCTCCTCTAGGGCCCTCAAGAGGAAAGGGC ATTGGGTCTAGCTTTGAAAGCTTCAGGATCAACATCCCTGGGAACACTGCGCACTGCAGCCCGCTGTCCA GCCCTGGATTCTGTAACACATTCCGGCCAGTAGACAGCAAGGTACAGAGGAAGGAGAACCCTTCACCCCT CTTCTCCATCAAGAAGCCGAAACAACTCAAAAGTGAAATCTATGACCCCTTTGATCCCACTGGCTCAGAC TCTAGCCCTCCTAGCAGCAGCCCAGAGAGTCTTGGTCCAGACCTTCTGCCCTCTGAGATCACAAGAACTA TCTCCATCAATAGCCCAAAGGCCCCAGCCTTCCAGACTGTGCGCTGTGTTACCTCCTACAGAGTAGAAAG CGTCTTTGGGACTGAGATGGACCCTGATCCCCAGCCCCCTGGGGAGCCTGTCTCTGGCATGCTGGAGTTA CTTGGCAAGGGACCTGCTGAGGGAGCCTCTGATTTGGAGCAAGAAGGACTAGGTGAAATAGAGCCTACAG AAATTCAGGGCTCCTCAGCCCGAGCACAGAGGCCATCCCCACCAGACCCTTGGGATGATGAGGATGGAGT GTCCTGTACGCCCTTCTTTGGCTCTGAGGAGCGGACAGTGACATGTGTGACTGTCGAGGAGCCAAGTGTT CCAAGCCCAGATGCTCCACAGATAACCACCCACAGAATTGTGGAGTTCAGAGCCTCATCCCGTTCCCGCT CCACCTCCAGCTCCCGAAGCAGGAAGAAAACAAAGAAGAAGAAGAAGGTTGCCAGAGAGCACCAGAGGAC ACGATCCAGCACTCGCTCAGGCTCCAGGGACAGGACTTCACGCTCAGTATCTCCATTTACCGAGGAACAC ACCAAGAGACACAGAGCCAAGACTAAGAGCCGGAGGTCTTCCAGTGACCGTGCCAGCAGCCAGGACAGAG CAAAGAGGAGGAAGGACAGAGACAGGGAGCATAGACGGGGTCCCTGGGGTCATGGCAGATGCTGGAGGAA GTCCAGGTCCCGCTCAGGGAGTCCTGGCAGTTCCTCCTGTGAGCGCCATGAGAGTAGGAGACGGAAGAGG CGGCATTCAGGGTCCAGATCTCGTGGCAGGGATGGCTCACCACACAGCAGCCTGGAGAGGGACCGGAGAC ATAAACATCGGGAGAGGAGCCGAGAGCGGATGGACAAGAAGGAGAGTATGACTCGGTCCCGGGAGAGAAG GAGGTGGCGGTCTCGGTCACCCAGTGTGGAGCATAGGACACGAAGGCCACATTCTCGTGAGAAGCATCCC CATTCCCCAGAGAAGAAGGGGGCTGTGAGGGAGGTTTCCCCAGCCCCTGCTCCACAGGGGGAGCCACGGC AGGATGGAGACCACTCTACCAAGCCTCCAGTCTCAGAAGTAAGTGTCTTGCCAGAGGTAGTAAGTGTCCT GCCAGAGGTGGTTGTGGCTGACCTGAACCCTCCAGAAGTCCCTCCTGTCCTCGCAGAGTCAGTTTCATGT GTGCCTGAGGACCTTGATTATGGTGATTCTGTGGAGGCAGGCCACGTCTTTGAGGATTTTTCAAATGAAG CCATCTTCATCCAGCTCGATGATATGAGCTCACCTCCTTCCCCTGAGAGTACAGACTCCTCCCCTGAGCG AGACTTCCTACCAAACCCCATACTGCCCCCAGCCAGCCTTCCACAAAACAGTACTTTACCTGTCACCCAG AGGGAGGTGTTACCAATTCACAGTGAAGATATCTCAAAGCCTGCACCCCAGCCATTGGCCCCTTCAGACC AGTGCCTGCTCAGGCAGGACACTGTAGAGACCACTGCTACAACTCTGAGCACCCCAGGTGTGTTGCCCAT GGGAAAGGACAGTCCTTTGCTGAGTGGGAGAGGATGTGAAGTGGTCAGGCCCAAGGATGCTGTGGCCCCG GCCCCACTGCTGCGATCCAGAACCCTGGTGAAAAGAGTCACCTGGAACCTGCAGGAAGCAGAGGCCAGCA CCCCAGCCCTGGACAGAGATCCAAGGACACCACTTCAGAGGCCCCAGAGGCCCCAGGAAGGAGACTGGGA TGCAGAGGACAGGGCCCTCATAGGATTTCAGCAGGCACCATTCTCCGAGCTGCCCCCTCCCATCCACGTG CTCCAGGAGTCTGGGTTACCTGATGCAGATCCCTCTCAGCCCCCTGGTGTTCCCAGGGCAGAAGGGCCTC CAGCTGTTGGGACTCTGCACTCAGCAGGGGGTATTCTTGCCCAGGTTTACAGCCCCAACATGCCACCACC CCTGGCTCAGCCCTCAAGCATCCCGCCCTATGCACTGGTCAACCAGCCCTCAGTCCAGTTGATTCTGCAG GGGACCCTTCCCCTAGCAGGCTGTGGTGCAGCACAGAACCTGGCCCCAGTGCCCACTATGCCAGCTACAG CCTCAGAGCTAGCTGTTCCAACCACCAACAACTCAGAAGAAAGGACGGCTACTCCCAAAACAGCTGCTGA GAAGACCACAAAGGAGGAGTACATGAAGAAGCTGCACATGCAGGAACGGGCTGTGGAGGAAGTGAAGCTG GCCATTAAACCCTTCTACCAGAAGAGAGAGGTGACCAAGGAGGAGTACAAGGACATCCTGCGCAAAGCTG TGCAGAAGATCTGCCACAGCAAGAGTGGCGAAATCAACCCTGTAAAGGTGGCCAACCTGGTGAAGGCCTA TGTGGACAAATACAGGCATATGCGCAAGCACAAGAAGACTGAAGCTGGAGAGGAGCCACCCACTCAAGGT GCTGAGACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_139093
Insert Size	4422 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_139093.1, NP_620793.1
RefSeq Size	5221 bp
RefSeq ORF	4422 bp
Locus ID	245925
UniProt ID	Q63625
Gene Summary	binds the repetitive C-terminal domain (CTD) of RNA polymerase II; may act with other CTD binding proteins to link transcription and pre-mRNA processing [RGD, Feb 2006]

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