Wdyhv1 (NM_001025024) Rat Untagged Clone

CAT#: RN211157

Wdyhv1 (untagged ORF) - Rat similar to hypothetical protein FLJ10204 (RGD1311362), (10 ug)



  "NM_001025024" in other vectors (3)

CNY 3990.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1311362
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211157 representing NM_001025024
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGGGACAGCCGCGCCGCCACGGCCTCGCAGTACCAGCCGGCCTGCCCTACGCGGGACGCATGTG
TCTACAGCAGCTGCTATTGTGAGGAAAACATTTGGAAGCTCTGTGAATACATCAAAACACACAACCAGTA
TCTGTTGGAGGAGTGCTACGCTGTCTTCATATCGAATGAGAAGAAGATGGTACCTATTTGGAAACAGCAG
GCAAGACCTGAGAATGGACCTGTGATCTGGGATTATCATGTCGTTCTGCTTCATGTGTCAAGGGAAGGAC
AGAGCTTCATTTATGACCTTGATACTATTTTGCCATTTCCCTGCCCTTTCGACATTTACATAGAAGATGC
CCTTAAGTCTGATGATGACATTCATCCACAGTTTAGGAGGAAATTCAGAGTGGTTCGTGCTGACTCCTAC
TTGAAGAACTTTGCTTCTGACCGGTCTCACATGAAGGACTCGAGTGGGAACTGGAGAGAGCCCCCTCCAG
AGTACCCCTGCATTGAAACTGGAGACTCCAAAATGAACCTGAACGACTTCATCAGCATGGACCCTGCAGT
AGGATGGGGAGCTGTCTACACGCTGTCTGAGTTTGTACATCGGTTCAGCAGCAAAAACTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001025024
Insert Size 624 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001025024.1, NP_001020195.1
RefSeq Size 1442 bp
RefSeq ORF 624 bp
Locus ID 362914
UniProt ID Q5BJV9
Gene Summary Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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