Otog (NM_001106262) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215301 representing NM_001106262 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCCGAGCGCCCAGACTCTGCTTGCTTCTCTCTTGCTTTTGTCTACTTAGGGTTGAGCCTGCTG CGGAGTTGGAGTTCAAGCGCACGGTGCAGCACCAGGCCTCAGCACTTACATGGAAAGCCGGTGAGGAGCT GCAACAGGAGCCTGCTCCTGAGCCCAGCCACAGCTACCAGATGCCCTTTGCAGTAGAAGATGTCACTACA GCTATGGAAGGCCAGGATGCTGAAGCCTTCGATGCACCGGCCATGTCTTCATGGGAAAGGCGGCTCCACC GGGCCAAGTGTGCGCCGTCTTACTTGTTCTCCTGCTTCAATGGGGGTGAGTGTGTGCACCCAGCTCTCTG TGACTGCAGACGCTTCAACGCCACTGGACCCCGTTGCCAGTTGGTGTATAATGCTGGTCCTGAGAGGGAC AGCATCTGCCGGGCCTGGGGCCAGCACCATGTGGAGACGTTTGACGGCCTGTACTACTACTTTTCTGGAA AGGGAAGCTACATGCTGGTGGGACACCATGAACCTGAGGGTCAGAGCTTTTCCATCCAGGTGCACAATGA CCCTCAGTGTGGCCCCGCCCACTACACCTGCCCAAGGTCTGTCAGTCTCTTCCTCTCGGGTGAACGTGAG ATCTGCCTGGCCAAGGAGGTCACCCACGGAGGTATAAGGATCCAGCTGCCACAGGTGGTGGCGGGTGTGC AGCTGCAGCAGCTGGCTGGTTATGTCATTGCGCGGTACCCATCAGCCTTCACGCTGGCCTGGGATGGTGC CTCAGCTGTCTACATCAAGATGAGCCCCGAGTTTGTGGGCTGGACCCATGGGCTGTGTGGGAACAACAAT GCCGACCCTCAGGATGACCTGGTGACCAGTTATGGGAAGCTGACTGATGACATAGGGGAGTTTGTGCACA GCTGGCAGGAGCAGGTCCCAAACAGCCCTCCAGTACCTGTGATGTCTTCCCTGCCTCGCCCACCATGCCT GCAGCAGAGTCCAGCGTCCATGCAGGGTGTGTATGAGCGCTGTGAAGTACTGTTGAGGTCTCCCTTCGAT GCCTGCCATGCCTATGTCAGCCCTCTGCCCTTCACAGCCAGCTGTACCAGTGACCTCTGCCAATCAGGAG GTGATGAAGCCACTTGGTGCCGAGCACTGACGGAATATGCCCGGGCCTGTGCACAGGCGGGGCGCCCTCT GCAGGGCTGGAGGACCCAACTCTCACAATGCACTGTGCGCTGCAAAGAGAAGATCTTCATCTACAACGAG TGTATTGCTTGCTGTCCAGCTTCCTGCCAATCCCATGCTTCATGTGTGGACAGCGAGATCGCCTGTGTGG ATGGCTGCTACTGCCCCAATGGTCTCATCTTTGAGGATGGGGTCTGCATGTCACCAGCCGAGTGTCCCTG TGAGTTCCATGGGACCCTGCACCCGCCTGGCTCAGTGGTGACAGAGGACTGCAATACCTGTACGTGCACT GCAGGGAAGTGGGTGTGCAGCTCTTCTGTCTGTCCAGCTGAATGCTCTGTGACTGGCGACATCCACTTCA CTACCTTTGATGGACGCCGGTACACATTCCCTGCCACGTGTCAGTACATCCTAGCCAAGAGCCGCTCCTC AGGCACATTTACCGTGACGTTGCAGAACGCCCCGTGTGGCCTGAACCAGGATGGGGCCTGTGTCCAGTCA GTGTCGGTGATTCTGCACCAGGACCCTCGGAGACAGGTGACCCTGACCCAGGCAGGAGATGTCCTTCTGT TTGACCAGTACAAGATCACCCCACCCTACTCAGATGACGCTTTTGAGATCCAGAAGCTGTCCTCTGTGTT CCTGAGGGTGAGGACCAATGTGGGTGTGCGGATCCTCTATGACCGGGAAGGGTTGCGACTCTACCTGCAA GTGGACCAGCGATGGGTGGAGGACACCGTGGGCCTCTGTGGCACCTTCAACGGCAACACACAGGACGACT TCCTGTCTCCAGTGGGGGTCCCTGAGAGCACCCCACAACTGTTTGGCAATTCTTGGAAAACCCTGTCTGC CTGTGTCCCGCTGGCCCCTGGCTCCTTGCTGGACCCCTGTGATGTGCATCTGCAAGCTGCATCGTATGCG CTGCAGGCCTGCAGCGTGCTCACAGGGGAGCTCTTTGCACCCTGCTCTGCATATCTGAGCCCGATTCCCT ACTTCGAGCAATGCCGTAGGGATGCCTGCCGCTGTGGGCAGCCCTGCCTGTGTGCCACACTGGCCCACTA CGCACGGCTATGCCGGCGCCATGGGCTCCCGGTTGACTTCCGTGCCCGTCTGCCAGCCTGTGCGCTGTCC TGTGAGGCCACTAAGGAGTACAGCCCTTGTGTGGCCCTGTGCACACAAACCTGCCAGGACCTGGCCAGCC CTGATGTCTGCGGGGCTAACGGTGGTGGCAACTTCAGCAGGGATGAGTGTGTGGAGGGTTGCACCTGCCC ACCGGACACATTCCTGGACACACAGACTGACCTCTGTGTCCCCAGGAATCAATGTTCTTGCCATTTCCAA GGAGTGGACTATCCCCCTGGGGACAGTGACATCCCTTCTCTGGGCCACTGCCACTGCAAAGATGGCGTCA TGAGCTGTGACAGCCGAGCCCCAGCTGCTACCTGTCCAGCAGGTCAGGTGTTCGTGAACTGCAGTGAGCT GCACCCAGACCCCGAGCTCAGCAGGGAGAGGACGTGTGAGCAGCAGCTGCTGAACCTGAGCCTGCCAGCA CGTGGTCCCTGCCTGTCGGGTTGTGCCTGTCCCCTGGGCCTGCTCAGACATGGGGATTCCTGCTTCCCAC CAGAGGAGTGTCCATGTACCTGGAAGGGGAAGGAGTATTTCCCTGGGGACCAGGTAGTCTCTTCCTGCCA TACCTGTGTGTGCCAGCGGGGCTCGTTCCAGTGCACCCTGCACCCCTGTGCCTCCACCTGCACTGCCTAC GGGGACCGACACTACCGTACATTTGACGGGCTTCCGTTTGACTTTGTAGGGGCGTGCAAAGTGCACTTGG TCAAGAGTACATCGGAGCTCAGCTTCTCTGTTATGGTGGAAGATGTGAACTGCTACAGCGCAGGTGTCAT CTGCAGGAAGTCCATCTCCATCAACGTTGGCAGCTCACTCATCATCTTCGATGACGACTCAGGGGATCCC AGCCCTGAGAGCTTCCTGGATGAGAAGCAGGCTGTCCACATCTGGAGAGCAGGCTTCTTCACGTTGGTGC ACTTCCCACGGGAACACATCACCCTCCTGTGGGACCAGAGAACCACTGTGCATGTCCAGGCGGGTCCTCA GTGGCAGGGTCAGTTGGTGGGCCTCTGTGGGAACTTTGACTTGAAAACCGTCAATGAGATGCGGACCCCA GAGAACCTGGAGTTAACAAACCCCCAGGAGTTTGGCAGCAGTTGGGCTGCGGTGGAGTGCCCAGACACTC CTGATCCCAGGGACACTTGTGTCCTGAATCCTCTCCGAGAACCATTTGCCAGGAAGGAGTGTGGTATTCT GCTCAGTGAGGTGTTTGAGACCTGCCACCCTGTGGTTGATGTCACTTGGTTTTACTCAAACTGCCTGACG GACACATGTGGCTGCAGCCGGGGTGGTGACTGTGAGTGCTTCTGTGCTAGTGTGGCTGCCTACGCCCATC AGTGCTGCCAACACGGGGTGGTAATTGACTGGCGGACACCCCGCATCTGCCCTTATGACTGTGACTTCTT CAACAAAGCGCTAGGTAAGGGTCCCTACCAGCTGTCCAGTGTGATGGCTGGCAGTGCTCTTGTGGCCATG AGGGCAGTAGATGGAGACATAGCCCTTGTGAGAGCAGAGGACCTGGTGCCAGGGGACATTTCAAGCTTCC TGCTGACAGCTGCCCTGTCCAAGGCCAAGGCGCATGACCCTGATGTGGTGTCCCTGGAGGCGGCTGACAG ACCCAACTTCTACCTGCATGTCACAGCCAATGGCTCTATAAGGCTGGCAAAATGGCGGCGCGATGAAGCC TTCCATCAGCATGCCTCCTTCTCCCTGCACCGGGGGACATGGCAGGCTGGCCTGGTGGCTCTGGAGTCCC TGGCAAAGCCTGGCTCCTTTCTCCAATCATCAGGCCTTAAGCTGGCTCTGCGGACATATGAACACACAGA GGCATTTCGTGGTGGTGCACTCTTCCGCCTTCTGGATGCCAAGCCTTCGGGGGCTGCCTACCCCATCTGT GAGTGGCACTATGATGCCTGCGCTAGCCCCTGTTTCCAAACCTGCCGGGACCCGCAGGCAGCCAGCTGCC AGGATGTGCCCAGGGTAGAAGGCTGTGTCCCTGTGTGTCCCACTCCTAAGGTCCTGGATGAAGTCACACA GAGATGTGTCTACCTGGAAGACTGTGTGGAGCCAGCCCTGCGGGGTCCCAAGGAGACCCTTGGTAATGAG ACTCTGGCACCTGGTCAAGTGCTTCCCACCACCAGTGACAAGCAGCAGCTTCCACAGGAGCCCCCTGGAG CCTCCACCCACATCCCTGTCCTTGATCCACTAGCCCCACTCACCTCAGCCCCAAATCCACCAATGGCTGC CACTGAGGGGCCAGTGGCATCTCCAGGATCCACCCAACCCACGCTGCAGACATCACTGGGGCTTACAACA TCTAACCTTCCTGCTGGCCACACAGAGGCCACAGCCAGAGAGGAAGGGGCTGCCAGCCTCCTAGCTACTT CTCCTCCCCCAGGGTCCTCCTCATCCCTCCCCAGTTCTCTGCAGACACCAACATCTGGCATTTTATCAAG TACAACAGAGACAACCAAAGTGACCTTAACCTTTGCAGGGAGTCCCAACACCACCGTCACTTCCCGGTCA CCCCCCGTACCTCGCTTCCCACTCATGACCAAAGCAGTGACAGTCCCAAGTCATGACTCCTTTCCTGTTA AAACCACACCCCTGCGGCCCTCTTGGAGCCTCTCTTCCAGACCTACGACTTCCCCTGGAGTGACGTCCTG GCCCCCCACCTCCCTGGGATCCCACCTGTCAACTGTAGTAACTAATGTGACAAACAAGACAGAGACATCC CTGTCTGTCCTGGCCCAGAGCACTAAAAGTTCCAGCCAGCCCCTGAGCACAGCATACAGGGCTCCTGCCA GTCCTCTTGTCACCAAGGGCTTAGAGGGAGCGTCAGCCACAGAGAAGGGAGACGCTGGGCACAGTCAGCT TACAGAGTTGCCAGTGTCCCCGCCCCCAAGCTCAGCCCCCATTGACCTGCCTCACCCAGCCCAACATACC ACCACAGCCCCTGGGTCTCCAGCTCTGTCCCCAGGGATCCTGGGTGCTGGCAGCCCATCCACAGGTGCTC ATGGACTGGGGACCACAGCTTTAGCTTCTCTAGAGACAACTCGGCCACCCCACCTCCTCTCTGGCCTGCC TCCTGACACCAGCCTGCCCTTGGCTAAGGTGGGCACATCTGCCCCAGTGGCCACACCTGCCTCCAAAGGT TACGTTCCCACCCCTCCCCCTCAGCACTGGGCTACAACTTCAGCCACAGCCATGACTGTGACCCCACTTA CTCAGACGACTCTCAGTCTGACAGCGCCACTCATGTCTGCTGTGGAGGAACAGGCACACTCACCTAGCCC CACAGCTCCCCACATAACAGCTATGACTCCAAGCTTTGGCGCCTCAGCAGGGACTGCTGGGGTGCCCCCC ATGGTGTCTGTTGCCCCGCAGAAGAGCACCACCCAGAGAGCAGCCATTCTCTCTAAGAAGGTGTCTCCAC CCACCCTCATCTCTGACTCTGTCCAGGGTGGGTTCACAGAACTCACGCCCACAGTGAGCCACACCGTTGC TCCCTTGGTGACTGAGGCTGAAGGCCCCCAGGCTGGCACAGTACCATTGGTGCCCACAACCCACTCCCTA AGCCGGGTGTCAGCCAGGACTGCCTCCCGGGAGGGCCCACTCGTTCTGCTGCCTCAGCTGGCAGAGGCCC ACGGGACCCCAGCAGGGCTCCAGCCCCAAGAGGATCTAGTGCGTCAGGCCACCACGGAGCAGACAGGGCG CTCAGCTCCAGCCCAGAGCATTGCAGAGGGATCGATGGAGGCCGAAGGGAACACTTCTGCCATCTGTGTG CCCATCGCCGAACAAGACTGCGTCCGTCACATATGCCTAGAAGGCCAGCTAATCCGTGTGAACCAGACCC AGCACTGTCCTCAGGGTGCCGTACGCCCTCGCTGTGGGGTCCTGGGCCTCGCTGTCCGGGTGGGCGGAGA TCACTGCTGCCCACTCTGGGAATGTGCCTGCAGATGCTCAATCTTCCCAGACCTGAGCTTTGTGACCTTT GATGGCAGCCATGCCGCTCTGTTCAAGGAGGCCATCTATATCCTCAGCCAGAGCCCAGACGAAATCATCT CTGTGCATGCCCTGGATTGCAAGAGTGCCAACCTGGGACACCTGAACTGGCCCCCATTCTGTCTGGTGAT TCTGAATGTGACTCACCTAACCCACCACATCAGCATCGACCGCTTCAACAGGAAGGTGACCGTGGACTCT CAAGTTGTGTGGCCCCCCATGAGTCGATATGGCTTCCGAATCGAGGATACGGGACACATGTATATTGTCC AGACACCCTCACACATCCAGATCCAGTGGCTTCACAGCTCAGGACTCATGATCCTGGAAGCCAGCAAAGC CAGCAAGACCCAGGGCCACGGCCTGTGTGGTATCTGTGATGGAGACACAGCCAATGACCTCACCCTGAAG GATGGCTCTGTGCTGGGCGAGGCAGAGGACCCTGCTCTCTTTCTGGATAGCTGGCAGGTGCCCAGCTCCC TGACCTCAGAGGGTCAGACCCGCTTCCGCCCAGACAGTTGCTCTACAGCGGACTGCTCGCCCTGCCTTCG CATGGTGTCCAACCGCACCTTCAGTGCCTGCCACAGCTTTGTCTCCCCAGAGTCATTCTGTGAGCTGTGG ATCCGGGACACCAAGTACGTGCAGCAGCCCTGTGTGGCCCTCACTGTGTATGTGGCCATGTGTCACAAAT TCCACGTGTGCATTGAGTGGCGGGGCTCCGACTACTGCCCCTTCCTGTGCTCCAGTGACTCTACCTACCA GGCCTGTGTGGCAGCGTGTGAACCCCCTGAAACGTGCCAGGATGGGGTACTGGGCCCACTGGACCCAGAG CAGTGCCAAGTGCTGGGTGAAGGCTGTGTGTGCTCCGAGGGCACCATTCTGCACCGACGCCACTCCGCAC TCTGCATCCCGGAGGACAAGTGCGCCTGCACTGACAGCACAGGGGTACCACGTGCTCTGGGGGAGACCTG GAACAGCTCCCTCAGTGGCTGCTGTCAGCAGCAGTGCCAGGCCCCAGACACCATCACACCCATGGACCCG GACTGCCCTGGTCCCCGACCTGAGAGCTGCCCACGCTTTGGAGAAGTGATCCTGTTACAGCCCACAGAGG ACCCCTGCTGCCTGGGGAGTGTCTGTGTGTGTAACCAGACCCTGTGCGAGGGCCTCGCTCCTACCTGCCG CCCAGGCCACAGACTCATCCCCCACTTCCAGGAAGACTCTTGTTGTCCCAGCTACAGCTGCGAGTGTGAC CCTGGTCTGTGTGAGGCTGAGCAGGTCCCCACCTGTCGCCAGGACCAGGTCTTGATCGAGGGTCGCCTAG GAGACTCCTGCTGTCCCTCCTACTTCTGTGGCTGTGGTGAGTGTCCAGATGCCATGCCTGAGTGTCAGGA AGGAGAGGTGCTCACTGTGCACAGGAATAGCACGGAGCTCTGCTGCCCTCTGTATCAGTGCGTGTGTGAG AGCTTCCGCTGCCCACAGATACAGTGTGGCATGGGCACTTCCCTGGTGGAGGTGTGGAGCCCAGATCGCT GCTGCCCCTACAAGTCCTGTGAGTGTGACTGTGACACTATTCCTGTGCCCAGATGCCATCTGTGGGAAAA GTCGCAGCTGGATGAGGAGTTCATGCACAGCGTGGAGAACGTGTGCGGCTGTGCCAAGTACGAGTGTGTG AAAGCCCCCGTGTGCCTGAGTCGTGAACTGGGTGTGATGCAGCCAGGCCAGACTGTGGTAGAGCTCTCGG CGGATGGCGTGTGCCATACGTCCCGATGCACAGATGTGCTGGACCCTCTCACCAACTTCTACCAGATCAA CATCACCTCCGTCCTTTGTGACATGCACTGTGAGGCGAACCAGGAGTATGACCACCCACGGGACCTGGCG GCCTGCTGCGGCTCCTGCAGGAACGTGTCCTGTCTCTTCACCTTCCCCAATGGCACCACTTCTCTGTTCT TGCCTGGTGCATCCTGGATTGCAGATTGTGCCCGCCATCACTGTGGCAGCACTCCCCTGGGTGCTGTCCT GGTTCGCTCGCCCATCAGCTGCCCTCCGCTCAATGAAACTGAGTGTGCCAAGGTCGGCGGCTCTGTGGTA CCATCTTTGGAAGGATGCTGCCGGACGTGTAAGGAGGATGGGCGATCTTGCAAGAAGGTGACCATCCGCA TGACCATTCGCAAGAATGATTGCAGGAGTAACACCCCTGTGAACCTGGTGTCTTGTGATGGAAGGTGCCC TTCTGCCAGCATCTACAACCACAACATCAACACCTATGCCCGCTTCTGCAAGTGCTGCCGTGAGGTGGGA CTGCAGCGCCGCTCTGTGCAGCTCTTCTGTGCTACGAATGCTACCTGGGTGCCCTACACAGTACAGGAGC CTACGGACTGTGCCTGCCAATGGTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106262
Insert Size	8709 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106262.1, NP_001099732.1
RefSeq Size	10037 bp
RefSeq ORF	8709 bp
Locus ID	292918

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.