Urb2 (NM_001135708) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1307980
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN215340 representing NM_001135708 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGTTTATTCTGGTATTTCCTTTAAACTTAAAAGCAAGACAACTTCCTGGGAAGATAAACTAA AATTAGCTCACTTTGCTTGGATTTCTCATCAGTGCTTTCTACCAAATAAAGAACAAGTTTTACTTGATTG GGCAAGACAGTCATTGGTTGCATTTTATAAGAAGAAGCTTGAACTGCAGGAAGATATTGTTGAAAGGCTC TGGGTCTATGTAGATAACATTCTACATAGCAGGAGATTACAGAATCTCCTCAAGAATGGAAAGACTATAA ATCTTCAGATTTCCCTTGTCAAGATCATAAATGAGAAAATCGAAGAGTTCTCACTTTCGGGATCCCAGAG GAGTATCTGCGCCATCCTGAGTTGCTGCCAGGGCATCCTCTCAGCACCTGCCCTTGCTGTCATCTACACA GCCAAACCAGAGCTTATTGTGGCTTTGCTGAGCCAGCTCTGCTGGTCAGCCTGCAGACAACCAGAAGGAG CCATGACAGCCAAGCTATTTGAGGTCATTCATCTGGCTCTTGACCATTACCTCAAACTTCAGCAGCAACA AGCCAACCCTAGGCGTGTCTTTGGAGATATGACTGGCCACCTCTTCCAGCCCTGCCTAGTCCTGAGACAC TTGCTTTTGGGGGGCACATGGACACAGGCTAGTCAGGGTCAGCTATGGCAGGTACTGAGCCGAGATATCA GGAGTAAGATTGATGCTGTTCTTCGAGGTGGCGTTTTTCGATATGATTTACTCTCATCCTATAAAGAGGA GCTCCTGGAACAGCAACAAGAGAACGTAAAGATGGGAGTCCTGAAGAATCTCCTCACTCCGATGGACACT GTGATTACGAGACTGGTGGAGCCTGACTATGTCAAATCAGACCTGCATGCTTTGGTTGTGGCCAGCTCAG TATCCTTGTTGTACAGACTCTTTCTGGACTCATACCTTAAGGAAGAAAACCAGTTTCTCTGCTTTCAGGT TCTCCCCAGGTTGTTTGGCTGCTTGCAAATTTCACACCTGCAGGAGGGACAGATGGAAGCCCTGTCCCTC TCAGATTGGACCACAGAGCTTCTGGCTGTAGAGCAACTACTAAACTTAGTGGCCACCAGTAACATCTACA ATGTAGCTGCTGACAGGATTCGGCATGGGGAGACACAGTTCCACTTCTACCGCCGTGTGGCTGAGCTGCT GATCAACCATTCACAAGCATCTGTGCCAGCTTGGTTTCGCTGCCTCAAGATTCTGATGTCTCTAAATCAT TTGATTTTAGAGCCAGACCTGGATGACCTGTTGTCTTCGGCTTGGATAGATGCAGAAGTAACAGAATTTC GAGCCAAAAAAGCCCAGGAGGTACTTATTAACACTGTCTTCCAGACGTATGCCAAGCTCCGACAGATGCC ACAGTTGTTTCAGGAGCTTTTAGAGGTGATCTGCCGTCCAGCTGCTGAGGCACTTCGACAGCCTTTGCTT GCCTCAGGCCTCTCTATGGCTCTCTGTGCTTGTTTTTTGGAGCTGCCACTGAGTCAGATCCTGGATACAT GGTCCCTTGTACTGGATAAGTTCCAGTCTTTAGTCATGCCCTGTTTGCAGAGTGATACTGATATGGCTTT TAAGGCAATGTCACTTAGCTCTCTGCTACACTGCATCATGTTCAACATGCAGAGTCTGGACAATAACATG CCTCTGCCCATCATCAGACGGACACAGTGCATGATGGAGAGGATGCTGAGAGAGCTTGTGAAGCCCCTTT TGGGCCTTCTCCTGGACCTCTGGAGCCCAGAGCCTGAGCCGTGGCAGCAGAAGGTGAGTGACTCTGCACT CTTACTCTCTTACACCTGGGCTCAGGTGGACACTACCCTGAGTTTGCACTGCAGCCAGTATTATTCTCTG GCAGTCTCCCTAGCCAGGGCTGCACTGGACAGCTCAAACCTCCCTTTGTTGCTACCAGGTGTGGAAACAG AGCTTTGGAAGAAGGTGGAAAAGTGTATAGCCCAGTCCAGGTCTCTCAGTAGGTACTGCTTAGAACAGCT GTACCTTCAGAAGGTGAAAAGGACTTTAATTCGGACTAGTTCCCAGTCCAAAGAAGCCCTTCAAACCTTG AGGTTTGACACTGCCCACATTCTTGATTCTAGCAGAGACTGTTTAAGTCAGAAGACAGTAACTGCCTGGG ATAGGCAGGTGTCGACGATGAATGAGTCCTTGTATCCTGTAGCACACTGGCACTTGATTGTATCAAACCT TACAGTTTTAATACCATATCTTTGTTTGAATGATGTGAGATATGTGGCCACTGTCTTGTTAAGAACTTTA CCAACAAGTAAAGCCCAGGGAAGCTTGGCACCTGGTGAGTCATATGTCACACTTGAGAAAATATCCACAG CCCTCCTTCACAGCCCTCTCTTTCCAGAGATGCAGTCCCTCTATTCTGCCTTCCTGACTTGCATCATTGC AAAATGTTCTAACATTCTGTGCTCTGGTGCGCATAATGATCTGAGTCTTAGTCAGCAGCTCCCCTGGCTT TTTGGAAAGGACTACCACACACTTGTGGCTCACTGGGAAACCAGATTGGCCAAAGTTGGATCTGAAGGTG TAGAACCAAGAGGAGAAATTGCCCAGAATTTTCTATCCATGGTCAAGAGTGGTTTTCCAATCAAGCTGCA TGAAGATCAACTGAAAGACCTCCTGGAGCTCTTCGATGTCATCTCTGCCCTCCACCTGGACAGCCTCTGG CCATCTTACCACGTGCATTTGTTTTTCCTGCTGTTCTCCATGGCCGTCTCCACATTAGGGCATTGCTCTT GCCCCCTAGCCCTCCAGTTCTTGGTGAAGTGCTACCAGCTCCTCAGTGGCCTGCAGAGAGGAAGGAACGC CCGCTCCGTATTCAGGGTCATGTACGTTAGTGACATCTTTGAGGTGGTGCTGACTTCACTGTTGCAAGCC AGTGCAGAGTTTCAAGTTAGGGAGGATGACCCTGCTTGGCTGCAGCTTCTTCAAGTGTCAGGGGTGTTCT TGGAACAGCTCATGCAAATGCTTATCCAAGCAAAGCTGAGCTTGGTGCTCAATTTTGGGAAAATCACTGC CTTTCTCTCCAAGTACAGTAAGGGAGCTTCCAGCAAAGAATTGAATATCCAGAACCTTCGGAGCAGGCAA CTGCTTCTGGTGGCTCTAACCAAATTGTGTCAAAGTTTGGGGCCTTGTGTTAAGGAGCGGAGGCAGCTAT TGGAGGCCCCAGCAGCACTCCCTGAGCTGCTGCAGCAGGCCATGATGCAGATGGGGGCCATGCTGCAGCT CTGCTTAGTGCCTGGGACTACAGGCTGCCGCCGCCGCCTGCCTTCAGTTCTCCTCTCAGCTGTCCCTACA CTTTTAGAAGTAGACCTGAGCCAGCACCTCAGGGAAGGACAGCCCAAAATTGCTCAAGTGGTGGATACCG ACAGAGCACTGCTTTCTCATGTGACACTTTACCAGGATGTCTACACTCAGTTGCTGGAGGAGTTGCCAGC CCTGTCGGGGAATACTCAGTCTTTCCAGGCGGCGTTGCGATTTCTAACCTTGTTCCTTTTGGCCCCAGAG TTCCATTCCAAGGAGAGCTCTGTTTTTGCTTCCATATTTTATTCTGTGCAGAAAGTTCTTACAGGTCCAT GCATACCTGCTCCAGTCACTCAGGATACTGAGCCTCATCTGGGAGCCCTGCTCACCCAAATGTTTAAGGC TGAGACAACAGAGCACTTTGGGATGGTACTGCAGTCTATTCTTCAGGGGCTGGATGTCACTCAGGCATGG AGGTCGGATCTACAGGTTGTTTTGTGTGCCATTAGACTACTCAACCTGCTGCTCAAATGTCCACTCAGTG GAGAAAAGGCGAGTTTGCTATGGCGTTCTTGCCCCCAGATAGTGACGGCTCTGATGCTGCAACACCGAGA GGCCTGCCAGGAGCAGCCTGTGGCCCTAGTAGTGGTTGAGCCTATTCTTGAAGTGTTGGCTGTTCTGCTT CGGAAAGGGGAGGAGAGCATCAGCAACCCTCACCATGTCAACCTGGCCTTTAACATCTTGCTCACAGTCC CTCTTGAGCACCTGAAGCCACGGGAGTTTGGCAGTGTCTTCCTGAAGACGCACAATGTGCTCTTCTCCAT CTTGCAGTGCCACTCTAAGGTGATGCTGAAGGCCATTCCGTCTTTCTTGAACTCCTTTAATAGACTGTTG CTTTCAGTGCTGCATGAAGGACGGCAGAAAGACCAAGGGAGCATGGATGACCTGCCAGTGGTCCTTGAAT GCGCACGCCTGGTGGAGAGGATGTATAGCCACATTGCTACTCGAGCTGAGGAGTTCACCACGTTCTCCCC ATTCCTGGTGGCCCAGTATGTGACAGAGGTACAGAAGGTAACCTTGTACCCACCTGTGAAGAACCTACTT CAGGAAGGAATTTATCTCATCTTGGATCTCTGCATGGAGCGGGATATCCAGTTTCTACGGGCTTCACTAC AGTCTGGAGCCCGAGATGTCTTTAAGGACCTGCACAGTGACTACCTCAAGTACCACAAGGCCAAGCATGA GGGAGAGAAGAGATATACGGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135708
Insert Size	4575 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135708.1, NP_001129180.1
RefSeq Size	5805 bp
RefSeq ORF	4575 bp
Locus ID	292087

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