Lrrc4b (NM_001271081) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Lrig4; NGL-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217130 representing NM_001271081 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGGCCCACATCCAAGGCTCCCCGTGCCCCTTGCTGCCGCCTGGTAGGATGTCCTGGCCCCAAG GGGCTCTCCTGCTCCTGTGGCTCTTCTCCCCACCCCTGAGGGCCGGTGGGGGCGGTGTGGCCGTGACTTC AGCCGCCGGAGGAGGCTCCCCGCCAGCCACCTCCTGCCCAGCAGCCTGCTCCTGCAGCAACCAGGCCAGC CGGGTGATCTGCACGCGGAGGGAGCTGGCCGAGGTGCCAGCCAGTATCCCCGTCAATACCCGCTACCTGA ACTTGCAAGAGAACAGCATTCAGGTGATCCGCACTGACACATTCAAGCACCTCCGGCACCTGGAGATCCT GCAGCTGAGCAAGAATCTGGTGCGCAAGATCGAAGTGGGGGCGTTCAATGGGCTTCCCAGTCTCAACACA CTGGAACTCTTCGACAACCGGCTGACCACAGTGCCCACACAGGCCTTTGAGTACTTGTCCAAGCTCCGGG AGCTGTGGCTGCGCAACAACCCCATCGAAAGCATCCCATCCTATGCCTTCAACCGTGTGCCCTCATTGCG CCGCCTGGACCTGGGCGAGCTGAAGCGTCTGGAGTACATATCGGAGGCGGCCTTCGAGGGGCTGGTGAAC CTGCGCTACCTCAACCTGGGCATGTGCAACCTAAAGGACATCCCCAACCTCACAGCACTTGTGCGCCTGG AAGAGCTGGAGCTGTCGGGGAACCGGCTGGACCTAATTCGCCCCGGCTCCTTCCAGGGCCTCACCAGCCT GCGCAAGCTGTGGCTGATGCATGCCCAAGTGGCCACCATCGAGCGAAACGCCTTCGACGACCTCAAGTCG CTGGAGGAGCTGAACCTGTCCCACAACAACCTGATGTCGCTGCCGCACGACCTTTTCACACCCCTTCACC GCCTGGAGCGTGTCCACCTGAACCACAACCCCTGGCACTGCAACTGTGATGTGCTCTGGCTCAGCTGGTG GCTGAAGGAGACAGTGCCCAGCAATACCACATGCTGCGCACGCTGCCACGCGCCCGCCGGCCTCAAGGGC CGCTACATCGGTGAGCTGGACCAGTCGCACTTCACCTGCTATGCCCCAGTCATCGTGGAGCCACCCACAG ACCTCAATGTCACCGAGGGCATGGCGGCCGAGCTCAAGTGCCGCACAGGTACATCCATGACCTCGGTCAA CTGGCTGACACCTAACGGCACGCTCATGACACACGGTTCCTATCGTGTGCGCATTTCGGTACTGCACGAT GGCACACTCAATTTCACTAATGTCACCGTGCAGGACACGGGCCAGTACACGTGCATGGTGACAAACTCTG CTGGCAACACCACTGCCTCGGCCACGCTTAACGTGTCGGCCGTGGACCCTGTGGCTGCCGGAGGGCCCGG TGGCGGGGGCCCAGGGGGTGGGGGCGGTGCAGGGGGTGCGGGCGGCTACACCTACTTCACCACGGTGACT GTGGAGACGCTGGAGACACAGCCGGGGGAGGAGGCCCAGCAGCCCCGAGGTACTGAGAAGGAGCCCCCGG GGCCCACGACTGATGGCGCGTGGGGTGGCGGCCGGCCTGACGCTGCAGCCCCGGCCTCAGCGTCCACCAC GGCACCAGCCCCGCGCTCCTCGCGGCCCACAGAGAAGGCTTTCACGGTGCCCATCACGGACGTGACGGAG AACGCACTTAAGGACCTAGACGATGTCATGAAGACCACCAAAATCATCATCGGCTGCTTCGTGGCCATCA CCTTTATGGCCGCCGTGATGCTGGTGGCCTTCTACAAGCTGCGCAAGCAGCACCAGCTGCACAAGCACCA TGGGCCCACGCGCACTGTGGAGATCATCAACGTGGAGGACGAGCTGCCCGCCGCCTCCGCTGTGTCGGTG GCTGCGGCCGCTGCGGTGGCTGGAGGCGCGGGCGTGGGTGGGGACAGCCACCTGGCTCTTCCGGCGCTGG AGCGAGACCACCTCAACCACCACCACTACGTGGCGGCTGCCTTCAAGGCGCACTACGGCGGCAATCCAGG AGGCGGGTGCGGGGCCAAGGGCCCCGGCCTCAACTCCATCCACGAACCTCTGCTCTTCAAGAGCGGCTCC AAGGAGAACGTGCAAGAGACACAAATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271081
Insert Size	2130 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271081.1, NP_001258010.1
RefSeq Size	2744 bp
RefSeq ORF	2130 bp
Locus ID	308571
UniProt ID	P0CC10
Gene Summary	Synaptic adhesion protein. Regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and PTPRF regulates the formation of excitatory synapses in a bidirectional manner.[UniProtKB/Swiss-Prot Function]

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