Nlrp9 (NM_001169149) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Nalp9a; Nalp9b; Nlrp9b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217406 representing NM_001169149 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACACGTCTGGATATGGCTTGCTGAAGCATCTGCGAAAACTGAGTGACGGGGAGTTCTGGAGCT TTAAAGAGCTTCTCAGGAAAGAGCCAGAGAAATTTAAGCTCAAACCAATATCCTGGATGAAAATTGAGAA TGCTTCCAAAGAAGAACTGGTAATGCTATTGAACACACATTATCCAAAACAGGCATGGGATATGGCGTTG AGCCTGTTTCTACAGGTCAATCGGGAAGACCTCTCAATCATGGCTCAGAAAAAGAGGAGATATAAACAAA CTAAATATAAAAAGTTTATGAAGACTACATTTCAAAGCATATGGACATTGGAGAGCAACATTTGTATACC TGATAGAAGCTACCATTTAATTGTAGAACACCAGTACAGAAAACTGCAGAACATATTTGATTCTGAATCA GAGCCAGTCACTGCAGTTGTAACAGGTCCAACAGGAGAAGGAAAAACCGTTTTCCTGAGAAAAGCAATGT TGGATTGGGCATCAGGAATTTTATGGCAGAACCGGTTTCAATATGTTTTCTTTTTCTCTGTCCTCTCACT TAACAATACCACAGAGTTAAGCTTAGCTGAGCTTATCTCAAGTAAGTTACCTGAGTCTTCAGAGACACTG AATGACATTTTATCTGATCCAAAGAAAATCTTGTTTATCCTGGATGGATTTGATTACCTGAAATTTGACT TGGAACTCAGGACAAACTTGTGTAATGACTGGAGGAAGATATTGCCAACACAAATCATCTTGAGTAGTTT ACTACAGAAAATAATGCTCCCAGAATCCTCTTTGCTTCTTGAATTAGGACATATAAGTTTACCAAAAATC TTCCCTTTGCTGCAGTATCCAAGGGACATAACTATCCAAGGATTCTCTGAGCGGTGTCTAAAGACCTATT TTATATCCTTCTTCAATACTGAAAAAGGCATAGAAGTCTTTGAGAATTTGAAGTCTAATCAAATGCTCAA ATTATGCAGCAATCCTTATCTATGCTGGATGTTCTGTAGTTGTTTAAAGTGGCAGTTTGACAGGGAAGAG GAAGCGTACTTTCAGGCTAAAACAGATTCGGTCTTCTTCACAAGCTTTATGGTTAGTGCATTCAAATCAG CATATGCCAGTAATCCACCTAAACAAAACAGAGCCCAATTAAAGAGCCTGTGTACCTTGGCTGTAGAAGG AATGTGGAAACAGTTGTTTGTTTTTGACTCTGAAGATCTCAGGAGGAATGGTATATCTGAATCAGATAAG GCAGTGTGGCTGAGAATGAAATTTCTCCAGAATCATGATAACCACATTGTGTTTTATCATCCTACACTAC AGTTATACTTTGCTTCCATGTTCTATTTCCTCAAACAAGACAAAGACACGCATGTCCCAGTCATTGGAAG CATACCTCAACTCCTAAGAAAAATTTATGCTCGTGACCACACACAATGGCTCCAGATAGGGATTTTTTTG TTTGGACTCGCCACTGAGCAAGTTGCCTCCTTGCTCAAACCATACTTTGGCTTTATACAGCACAGAGATG TAAGACAGGAAGTCATGAGATACCTTAAGAGTCTCAGTCAACGAGAGTGCTGTGAGAAATTGGAAAGACC CCAGAATTTGTTTGCCTGTCTACGTGACAACAAGGAGGAAAAATTTGTAAGAGAAGTGGTAGATCTATTT GAAGAAATTACTGTTGATATTACTAATTCACATGTATTGTCAATAATTGCAAACCATCTTCAGAAATCCT CCAAATTAAAGAAACTTCACCTGCATATACAAAAGAGAGTTTTTTTAGAAATTCATGACCCAGAATATAG TGACTCAGAAACCTTTACACAGGACAAAAAAAATGCAGCTGAATATTGGAAGAAGTTATGTCATATTTTT GTCAACTTACATGTGTTGGATCTGGACAGCTGTAATTTCAACAAACAGGTCATAGAGGAACTCTGTAATG TGCTGTCTCCATCACCTAAGATACCTCTGATGGCTTTTAAGCTCGAAAGCTTGTTGTGTTCATTCATGAC TAACTTTGGAGATGGTTCACTATTTCACACCCTTCTTCAGCTACCTCACCTGAAATACCTAAACCTGTAT GGCACCAACCTCTCCAACAGTAGGATTGAGAATCTGTGCTCTGCTCTGAGACGCTCAACATGCAAAGTAG AGGAACTACTGTTGGGTAAATGTGACATATCAAGTGAGGCATGTGGCATAATTGCTACTTCCCTTATGAA CTCTAAAGTGAAACATCTCTCATTGGTTGAAAATCCCTTGAAGAACAAAGGAGTGATGTCACTGTGTGAA ATGCTGAAGGATCCAAGCTGTGTCCTTGAGACACTGATGTTGTCTTACTGCTGTCTCACATTCATTGCCT GTGGCCATCTCTATGAAGCCCTTGTGAGCAATAAACACCTCTCCCTGCTTGATCTGGGGTCAAATTTCCT GGAAGATACTGGAGTGAACCTTCTGTGTGAAGCTTTGAAAGACCCAAACTGCATCCTCAAGGAGTTATGG TTGTCAGGTTGCTTTTTAACTTCAGAATGCTGTGAGGAGATCTCTGCTGTCCTGACTTGCAATAATAATC TAAAGACCCTGAAACTGGGCAACAATGACATAGAAGACACTGGTGTCAAACATTTATGTGAAGCCCTGAG TCATCCTAACTGTAAATTAGAATGTCTTGGGTTAGACCTGTGCAAGTTCACAAGTGACTGTTGTGAAGAC CTGGCCTCAGCTCTCACCACCTGCAAAACACTGAATAGCTTAAATCTGGACTGGAAAACCTTGGAACACT CTGGAGTCGTGGCTCTCTGTGAGGCTTTGAATCACAAGAAATGTAACTTGAAGATGCTTGGACTGGACAA ATCTGCATTTTCTGTGGAATCGCAGACATTACTGCAAGCTGTGGAAAAGAAAAACAATAATCTGAGTATT TTACATTATCCCTGGGTTGAAGAGGAACGCAAGAAGAGGGGTGTTCGTCTGGTCTGGAACAGCAAGAATT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001169149
Insert Size	3012 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001169149.1, NP_001162620.1
RefSeq Size	3012 bp
RefSeq ORF	3012 bp
Locus ID	292577

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