Ints2 (NM_001100630) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1305547
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217541 representing NM_001100630 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCCTGAGTGCACAAGCCTTCAGTTTGTCAGCCCTTGTGCTTTTGAAGCCATGCAGAAGGTGGACG TCGTTCGCCTGGCATCTTTAAGTGATCCAGAACTAAGACTTCTCTTGCCCTGCTTGGTGAGGATGGCTCT TTGTGCTCCTGCCGACCAGAGCCAAAGCTGGGCTCAGGATAAAAAACTTATACTTCGCCTTCTGTCTGGA GTGGAAGCTGTCAACTCCATTGTTGCACTGCTGTCTGTGGACTTCCACGCCCTGGAACAAGATGCCAGCA AAGAACAACAGCTCAGGCATAAACTTGGAGGAGGCAGTGGAGAGAGCATCCTGGTATCACAGCTTCAGCA TGGACTGACTCTAGAATTTGAACACAGTGATTCCCCTCGTCGATTGCGGCTTGTGCTGAGTGAATTGTTG GCAATTATGAACAAGGTGTCGGAGTGCAGTGGGGAGTTTTTCTTCAAGTCTTCTGAGCTCTTTGAGAGCG CCGTGTACTTGGAGGAGGCTGCGGATGTCCTTTGTATCTTACAAGCAGAGCTCCCTTCCTTGCTTCCTAT AGTGGATGTGGCTGAAGCCTTGCTACATGTAAGAAATGGTGCCTGGTTCTTGTGTCTCTTGGTAGCCAAC GTTCCTGATAGTTTTAATGAAGTTTGTAGGGGCCTGATCAAAAATGGAGAACGGCAGGATGAAGAAAGCC TTGGAGGGAGGCGCAGGACAGACGCCTTACGCTTCTTATGCAGAATGAATCCTTCTCAGGCCCTGAAGGT CCGGGGCATGGTGGTGGAAGAATGTCACTTGCCAGGCCTTGGGGTAGCTTTGACATTGGATCATACCAAA ACTGAAGCTTGTGAAGATGGAGTGAGTGACTTGGTTTGCTTTGTCAGTGGCTTGCTTCTTGGAACAAATG CAAAAGTCAGGACTTGGTTTGGAACCTTCATTCGAAATGGACAACAGAGAAGAAGGGAGAACAGCGGCTC GGTGCTTTGGCAGATGAGAAGGCAGCTTCTTCTGGAGTTGATGGGCATTCTCCCCACAGTGAGGAGCACC CGTATTGTGGAGGAAGCCGATGTGGAGATGGAGCCTACTGTGTCGGTGTACTCGGGGCTGAAAGAAGAGC ATGTCGTCAAAGCCAGCGCACTCCTACGTCTATACTGTGCCTTGATGGGGATTGCTGGACTCAAGCCAAC TGAAGAGGAAGCCGAGCAGCTGCTGCAGCTGATGACCAGCCGGCCTCCCGCCACGCCCGCCGGGGTTCGC TTCGTTTCGCTGTCCTTCTGTATGCTGCTGGCCTTCTCCACACTTGTCAGCACGCCGGAGCAGGAGCAGC TGATGGTCGTGTGGCTCAGCTGGATGATCAAAGAAGAAGCCTACTTCGAGAGTACATCAGGTGTCTCGGC ATCTTTTGGGGAGATGTTGTTATTGGTGGCCATGTATTTCCACAGCAACCAGCTTAGTGCTATCATTGAC TTAGTCTGCTCCACTTTGGGGATGAAGATCGTGATTAAGCCAAGCTCCTTGAGCAGGATGAAGACCATCT TCACACAGGAGATCTTTACAGAGCAGGTTGTCACAGCCCATGCAGTGCGAGTTCCTGTCACCAGCAACCT GAGTGCCAATATTACTGGGTTTTTGCCCATTCATTGTATTTACCAACTTCTCAGGAGTCGATCGTTTACC AAGCACAAAGTGTCAATAAAAGACTGGATTTATAGACAGCTGTGTGAAACTTCCACTCCACTGCACCCGC AGCTGCTTCCTCTGATTGATGTGTACATAAACTCTATACTTACTCCTGCATCTAAATCTAACCCTGAAGC CACAAACCAGCCGGTCACTGAGCAGGAGATACTCAACATCTTCCAGGAAGTCATCGGGGGTGACAGCGTC CGCCTTAACCAGCGCTTCAGCATCACAGCGCAGCTTCTGGTGCTCTATTATATCCTGTCCTACGAGGAAG CTCTCCTGGCAAACACAAAGACGTTAGCATCCATGCAGAGGAAGCCCAAGTCGTATTCTTCTTCTCTAAT GGATCAGATTCCTATCAAGTTTCTCATTCGGCAGGCTCAAGGACTACAGCAGGAGTTGGGAGGGCTGCAT TCAGCTTTACTCCGTCTTCTAGCAACTAATTACCCCCATTTGTGTATCGTGGATGACTGGATCTGTGAGG AGGAGATCACAGGGACGGATGCTCTGTTAAGGCGAATGCTCCTGACTAGTAATGCCAAAACCCACTCTCC GAAACAGCTGCAGGAAGCATTTTCAGCTGTCCCTGTAAGTCACACACAAGTGATGCAGATTATGGAGCAC TTGACTCTACTTTCTGCCAGTGAGCTTATACCATATGCAGAAGTTTTAACGTCCAATATGAATCAGCTTC TGAATTCTGGTGTTCCACGGAGAATTCTTCAAACTGTCAACAAGTTATGGATGGTTCTTAATACTGTGAT GCCAAGAAGGCTGTGGGTAATGACGGTTAATGCACTCCAACCTTCCATAAAGTTTATACGACAACAGAAA TATACCCAGAATGATTTGATGATAGATCCTCTCATTGTCTTGAGGTGTGACCGAAGGGTCCACAGGTGTC CTCCACTAATGGATGTTACTTTACACATGCTGAATGGGTATCTCCTTGCCTCTAAAGCTTACCTTAGTGC CCATCTGAAGGAAACAGCAGAGCAGGATAGACCTTCCCCGAATAATACAGTAGGCTTGGTTGGACAGACT GATGCCCCAGAAGTGACCAGAGAAGAACTGAAAAATGCATTACTGGCTGCTCAGGACAGTGCGGCTGTCC AGATTCTCTTAGAGATTTGTTTGCCTACTGAAGAGGAAAAGGCAAAGGCTGCCAATTCAGACATCTCCCT CAGGAACACTCAGGGTGTCATTACCATTGGCACCCCAAATAAAGACACAGAAGAAGGAGAAGACAGTTTG CTTTGCAACCTTCGAGAAGTTCAGTGCCTTATCTGTTGCCTGTTGCACCAAATGTACATTGCTGATCCCA ACATTGCTAAACTTGTTCACTTTCAGGGCTATCCATGTGAACTTCTGCCTCTAACAGTCGCAGGGATCCC ATCTATGCACATCTGTTTGGACTTCATACCGGAGCTTATTGCCCAGCCGGAACTTGAAAAACAGATATTT GCTATACAGCTGCTTTCTCACTTGTGTATCCAGTATGCATTACCGAAGTCACTAAGTGTGGCTCGCTTAG CTGTCAATGTCATGGGGACTTTGCTAACAGTTCTAACACAAGCTAAGCGGCATTCTTTTTTCATGCCGAC TCTTCCAAGTTTGGTCTCCTTTTGCCGAGCATTTCCTCCACTGTATGAGGATGTTATGTCTTTGCTCGTT CAAATAGGACAAGTGTGTGCCTCTGATGTTGCCACTCAGACAAGAGACATTGATCCGATCATTACCCGTC TTCAACAAATAAAGGAGAAACCTAGCGGATGGTCTCAAATTTATAAAGATGCATCTTACAAACATGGATC CAGGGACACTGGAAGCATGGATCCTGATGTCCAGCTCTGTCATTGTATTGAAAGCACAGTAATTGAAATA ATAAACATGAGTGTTAGTGGGATTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100630
Insert Size	3597 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100630.2, NP_001094100.2
RefSeq Size	5915 bp
RefSeq ORF	3597 bp
Locus ID	360589

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.